• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.522-530
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• 2018

Brachypodium distachyon has been developed as a monocot model plant for temperate grasses and bioenergy crops. Although B. distachyon research is moving forward rapidly, the study of photoresponses has not been explored. To extend our knowledge of responses to light in monocots, we performed photoresponse analysis of B. distachyon using two inbred lines, Bd21 and Bd21-3. In this study, we first compared growing phenotypes between the two lines and investigated coleoptile and primary leaf growths under dark, far-red, red, and white light conditions. The results showed that the growth of the two lines were similar until tillering stage, but other developmental stages from heading to senescence were much delayed in Bd21-3, which resulted in increased height and tiller numbers. Under different light conditions, primary leaf lengths were kept increasing during the growth period, whereas the coleoptile extension was inhibited 4 to 7 days after growth depending on the light conditions applied. These results suggest that the responses to light in B. distachyon can be examined by measuring coleoptile lengths approximately 7 days after seedling growth. Moreover, we selected light-responsive genes known in Arabidopsis thaliana, such as chlorophyll A/B binding protein (CAB), light-harvesting chlorophyll binding protein (Lhcb) and chalcone synthase (CHS), and confirmed their light-induced gene expression in B. distachyon. Therefore, the present study suggests that the inhibition of coleoptile growth can be used as the parameter to analyze photoresponses in the monocot model plant, and also provide the reference genes whose expression is induced by far-red and red light treatment.

• Journal of Microbiology and Biotechnology
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• v.27 no.9
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• pp.1670-1680
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• 2017

Lignocellulose, composed mostly of cellulose, hemicellulose, and lignin generated through secondary growth of woody plant, is considered as promising resources for biofuel. In order to use lignocellulose as a biofuel, biodegradation besides high-cost chemical treatments were applied, but knowledge on the decomposition of lignocellulose occurring in a natural environment is insufficient. We analyzed the 16S rRNA gene and metagenome to understand how the lignocellulose is decomposed naturally in decayed Torreya nucifera (L) of Bija forest (Bijarim) in Gotjawal, an ecologically distinct environment. A total of 464,360 reads were obtained from 16S rRNA gene sequencing, representing diverse phyla; Proteobacteria (51%), Bacteroidetes (11%) and Actinobacteria (10%). The metagenome analysis using single molecules real-time sequencing revealed that the assembled contigs determined originated from Proteobacteria (58%) and Actinobacteria (10.3%). Carbohydrate Active enZYmes (CAZy)- and Protein families (Pfam)-based analysis showed that Proteobacteria was involved in degrading whole lignocellulose, and Actinobacteria played a role only in a part of hemicellulose degradation. Combining these results, it suggested that Proteobacteria and Actinobacteria had selective biodegradation potential for different lignocellulose substrates. Thus, it is considered that understanding of the systemic microbial degradation pathways may be a useful strategy for recycle of lignocellulosic biomass, and the microbial enzymes in Bija forest can be useful natural resources in industrial processes.

• Mycobiology
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• v.47 no.1
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• pp.87-96
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• 2019

Fungi produce various secondary metabolites that have beneficial and harmful effects on other organisms. Those bioactive metabolites have been explored as potential medicinal and antimicrobial resources. However, the activities of the culture filtrate (CF) and metabolites of whiterot fungus (Schizophyllum commune) have been underexplored. In this study, we assayed the antimicrobial activities of CF obtained from white-rot fungus against various plant pathogens and evaluated its efficacy for controlling anthracnose and gray mold in pepper plants. The CF inhibited the mycelial growth of various fungal plant pathogens, but not of bacterial pathogens. Diluted concentrations of CF significantly suppressed the severity of anthracnose and gray mold in pepper fruits. Furthermore, the incidence of anthracnose in field conditions was reduced by treatment with a 12.5% dilution of CF. The active compound responsible for the antifungal and disease control activity was identified and verified as schizostatin. Our results indicate that the CF of white-rot fungus can be used as an eco-friendly natural product against fungal plant pathogens. Moreover, the compound, schizostatin could be used as a biochemical resource or precursor for development as a pesticide. To the best of our knowledge, this is the first report on the control of plant diseases using CF and active compound from white-rot fungus. We discussed the controversial antagonistic activity of schizostatin and believe that the CF of white-rot fungus or its active compound, schizostatin, could be used as a biochemical pesticide against fungal diseases such as anthracnose and gray mold in many vegetables.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.162-162
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• 2022

Stormwater management is an essential component of land-use planning and development. Due to the additional challenges posed by climate change and urbanization, various stormwater management schemes have been developed to limit flood damages and ease water quality concerns. Nature-based solutions (NBS) are increasingly used as cost-effective measures to manage stormwater runoff from various land uses. Specifically, constructed wetlands were already considered as socially acceptable green stormwater infrastructures that are widely used in different countries. There is a large collection of published literature regarding the effectiveness or efficiency of constructed wetlands in treating stormwater runoff; however, metadata analyses using bibliographic information are very limited or seldomly explored. This study was conducted to determine the trends of publication regarding stormwater treatment wetlands using a bibliometric analysis approach. Moreover, the research productivity of various countries, authors, and institutions were also identified in the study. The Web of Science (WoS) database was utilized to retrieve bibliographic information. The keywords ("constructed wetland*" OR "treatment wetland*" OR "engineered wetland*" OR "artificial wetland*") AND ("stormwater*" or "storm water*") were used to retrieve pertinent information on stormwater treatment wetlands-related publication from 1990 up to 2021. The network map of keyword co-occurrence map was generated through the VOSviewer software and the contingency matrices were obtained using the Cortext platform (www.cortext.net). The results obtained from this inquiry revealed the areas of research that have been adequately explored by past studies. Furthermore, the extensive collection of published scientific literature enabled the identification of existing knowledge gaps in the field of stormwater treatment wetlands.

• Economic and Environmental Geology
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• v.40 no.6
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• pp.785-795
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• 2007

Recently, the knowledge and use of information production and service in integrated information has been changing from intra-organizational to inter-organizational and even international. In particular, since natural disasters are complexly related, it is increasingly important to use integrated information from related areas in the reduction and prevention of such events. In this study, we suggest strategies for the use of integrated information from natural disasters based on that expanding need. This study analyzes information user surveys, the interrelated information technologies, trends, and case studies to determine user access to the information, the status of the information used, and the supply and distribution of that information. From the survey results, we can draw conclusions about the effectiveness of the strategies of integrated information used in domestic natural disaster information.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2003.10a
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• pp.1-1
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• 2003

It is clear that computers will play a key role in the biology of the future. Even now, it is virtually impossible to keep track of the key proteins, their names and associated gene names, physical constants(e.g. binding constants, reaction constants, etc.), and hewn physical and genetic interactions without computational assistance. In this sense, computers act as an auxiliary brain, allowing one to keep track of thousands of complex molecules and their interactions. With the advent of gene expression array technology, many experiments are simply impossible without this computer assistance. In the future, as we seek to integrate the reductionist description of life provided by genomic sequencing into complex and sophisticated models of living systems, computers will play an increasingly important role in both analyzing data and generating experimentally testable hypotheses. The future of bioinformatics is thus being driven by potent technological and scientific forces. On the technological side, new experimental technologies such as microarrays, protein arrays, high-throughput expression and three-dimensional structure determination prove rapidly increasing amounts of detailed experimental information on a genomic scale. On the computational side, faster computers, ubiquitous computing systems, high-speed networks provide a powerful but rapidly changing environment of potentially immense power. The challenges we face are enormous: How do we create stable data resources when both the science and computational technology change rapidly? How do integrate and synthesize information from many disparate subdisciplines, each with their own vocabulary and viewpoint? How do we 'liberate' the scientific literature so that it can be incorporated into electronic resources? How do we take advantage of advances in computing and networking to build the international infrastructure needed to support a complete understanding of biological systems. The seeds to the solutions of these problems exist, at least partially, today. These solutions emphasize ubiquitous high-speed computation, database interoperation, federation, and integration, and the development of research networks that capture scientific knowledge rather than just the ABCs of genomic sequence. 1 will discuss a number of these solutions, with examples from existing resources, as well as area where solutions do not currently exist with a view to defining what bioinformatics and biology will look like in the future.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.2
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• pp.647-653
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• 2016

Background: Breast cancer is the most common type of cancer affecting Malaysian women. Recent statistics revealed that the cumulative probability of breast cancer and related deaths in Malaysia is higher than in most of the countries of Southeast Asia. Single nucleotide polymorphisms (SNPs) in CYP2E1 (rs6413432 and rs3813867), STK15 (rs2273535 and rs1047972) and XRCC1 (rs1799782 and rs25487) have been associated with breast cancer risk in a meta-analysis but any link in Southeast Asia, including Malaysia, remained to be determined. Hence, we investigated the relationship between these SNPs and breast cancer risk among Malaysian women in the present case-control study. Materials and Methods: Genomic DNA was isolated from peripheral blood of 71 breast cancer patients and 260 healthy controls and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: Our study showed that the c1/c2 genotype or subjects with at least one c2 allele in CYP2E1 rs3813867 SNP had significantly increased almost 1.8-fold higher breast cancer risk in Malaysian women overall. In addition, the variant Phe allele in STK15 rs2273535 SNP appeared to protect against breast cancer in Malaysian Chinese. No significance association was found between XRCC1 SNPs and breast cancer risk in the population. Conclusions: This study provides additional knowledge on CYP2E1, STK15 and XRCC1 SNP impact of risk of breast cancer, particularly in the Malaysian population. From our findings, we also recommend Malaysian women to perform breast cancer screening before 50 years of age.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.43-43
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• 2018

Mutation breeding by radiation is useful for improving various crop species. Up to now, a total of 170 soybean mutant varieties have been released in the world, which is the second most registered varieties after rice. Despite the economic importance of soybean, there have been no TRAP marker system studies on genetic relationships between/among mutant lines. To develop a strategy of Mutant Diversity Pool (MDP) conservation, a study on the genetic diversity of 210 soybean mutant lines (8 cultivars and 202 mutants) was performed through a TRAP analysis. Sixteen primer combinations amplified a total of 551 fragments. The highest (84.00%) and lowest (32.35%) polymorphism levels were obtained with primers MIR157B + Ga5 and B14G14B + Ga3, respectively. The mean PIC values 0.15 varied among the primer combination ranging from 0.07 in B14G14B + Sal2 to 0.23 in MIR157B + Sa4. Phylogenetic, principal component analysis (PCA) and structure analysis indicated that the 210 lines belong to four groups based on the 16 combination TRAP markers. AMOVA showed 21.0% and 79.0% variations among and within the population, respectively. Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. Our results suggest that the TRAP marker system may be useful for assessing the genetic diversity among soybean mutants and help to improve our knowledge of soybean mutation breeding.

• Journal of the Economic Geographical Society of Korea
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• v.16 no.1
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• pp.50-70
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• 2013

The creation of new economic paradigm shift in creative economy age have influence on the characteristics of social networks and space, it leads to the formation of new relationship in space depending on social network service development. In this paper, it gives a name to 'social network resource' the power affecting these features and to find the meaning of spatial changes in the economic geography perspectives. 'Social network resource' shows the characteristics of openness, sharing, participation and cooperation, with features of encompassing all the features of local and global characteristics in space. This features are related the meaning of 'trans-locality' and can be found in the case of 'WikiSeoul.com (http:/www.wikiseoul.com)', Seoul's social knowledge sharing web platform. In particular, physical resources, human resources, information resources, and the characteristics of the relationship as a resource features was found and these features appear in space is projected to the space of social relations, it reflects the characteristics of qualitative space regarding social network resource.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.15-15
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• 2018

민속식물과 그 이용에 관한 전통지식이 산업화, 도시화 및 노인 세대의 퇴장과 더불어 빠르게 사라지고 있다. 이에 본 연구는 2005년부터 2017년까지 1차(2005~2013), 2차(2014~2017)에 걸쳐 남한 전역 및 함경북도 접경지역을 대상으로 각 지역에서 전통적으로 이용해왔으며 전승되어 온 민속식물 관련 전통지식을 발굴 및 수집하여 DB를 구축하고 유전자원에 대한 주권확보 및 그 연구의 토대를 마련하고자 수행하였다. 조사 결과, 민속식물은 138과 557속 975종 7아종 113변종 19품종으로 총 1,114분류군으로 확인되어 1차 조사 대비 140분류군이 추가되었으며, 이는 한반도 전체 관속식물 4,881분류군의 약 22.8%에 해당한다. 용도별로는 총 115,236건의 전통지식 중 식용(67.2%)이 가장 많고 그 다음으로 약용(17.9%), 용재용(4.9%), 유지용(2.5%), 관상용(1.3%), 섬유용(1.2%), 사료용(0.7%), 염료용(0.7%), 향신료(0.5%), 연료용(0.4%), 천렵(0.4%), 살충제(0.2%), 향료(0.1%), 기타(1.9%) 등의 순으로 나타났다. 본 연구에서는 민속식물의 이용에 대한 중요도를 알아보기 위하여 특정식물 종의 이용을 언급한 정보보유자의 수(FC: Frequency of Citations), 이용범주의 수(NC: Number of use-Categories), 특정식물을 이용하는 수(UR: number of User Reports)를 활용하여 문화적 중요도(CI: Cultural Importance), 상대적 인용빈도(RFC: Relative Frequency of Citations), 상대적 중요도(RI: Relative Importance) 및 문화적 가치(CV: Cultural Value)를 산출하여 우리나라에서 중요한 민속식물을 정량적으로 확인, 비교하였고 각 분류군별 지수가 제시되었다. 정량적 분석 결과, 상대적 인용빈도(RFC)는 참취, 문화적 중요도(CI), 상대적 중요도(RI), 문화적 가치 지수(CV)는 모두 소나무가 가장 높은 민속식물로 확인되었다.