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Geographical origin discrimination of Korean variety, Angelica gigas Nakai by using TDU (Thermal Desorption Unit)-GC/MS (TDU-GC/MS를 이용한 한국품종 참당귀 국산 판별 기법)

  • Lee, Mi-Na;Kim, Yoon-Suk;Kim, Won-Il;Kim, Jeong-Gyu;Kwon, Oh-Kyung
    • Journal of Applied Biological Chemistry
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    • v.63 no.1
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    • pp.29-33
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    • 2020
  • The objective of this study was to develop a fast and accurate method of variety discrimination and geographical discrimination origin of Korean angelica (Korean variety, Angelica gigas Nakai) by using TDU-GC/MS. Two peaks of decursin and decursinol, which are coumarin derivatives were identified in the range of Total Ion Chromatogram (TIC) RT 26.9-27.2 of the Korean angelica by GC/MS analysis at the time of condensation in a refrigerated condensation system after thermal desorption of sample extracts. In case of Chinese angelica (Chinese variety), ligustilide peak was detected at the RT 17.2. In order to investigate the difference of volatile components according to the geographical origin of Korean variety, the mass spectra were measured by TDU-GC/MS at the range of m/z 40-400 amu. The TIC of domestic cultivation and Chinese cultivation of the Korean variety, Angelica gigas Nakai showed the same tendency as a whole. However, in partial scans of TIC, two peaks detected at 15.54 and 16.05 of RT showed different peak patterns between Korean angelica (Korean variety) cultivated in Korea and in China. The ratio of Peak A (RT 15.54) and B (RT 16.05) was 0.0-0.2 for domestic cultivation and 0.5-2.8 for Chinese cultivation, confirming the possibility of discriminating origin by comparing the TIC peak pattern of TDU-GC/MS.

Effects of Sewage Treatment on Characteristics of Sludge as a Composting Material (하수처리가 퇴비화를 위한 하수 슬러지 특성에 미치는 영향)

  • Kim, Jae-Koo;Kim, Jong-Soo
    • Applied Biological Chemistry
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    • v.41 no.2
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    • pp.181-186
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    • 1998
  • The effects of sewage treatment on characteristics of sludge as a composting material were investigated for a year during the initial operation at the full-scale Chunan sewage treatment plant. Due to the shortage of design capacity of belt press, a sludge dewatering unit, non-volatile solids were recirculating and concentrating in the treatment plant, resulting in an increase of MLSS and a decrease in F/M ratio at the activated sludge system. Special attention is required for long term operations since the increase of non-volatile solids in the plant would deteriorates the treatment efficiency. The sewage sludge of the Chunan sewage treatment plant showed 79.5% of water content, 11.6% of organic content, and C/N ratio of 6.1, and contained As 1.8 mg/kg, Cd 27 mg/kg, Hg <0.1 mg/kg, Pb 54 mg/kg, T-Cr 370 mg/kg, and Cu 1,100mg/kg of heavy metals. In order to be used as raw material for optimum composting, the sewage sludge requires bulking agents for moistrure/porosity control and a carbon source for adjusting C/N ratio. However, the sewage sludge is not adequate as a soil conditioner after composing due to a high content of heavy metals. If the sewage sludge has to he used as a soil conditioner after composting, it as required to identify and remove tire industrial wastewater portions in tire influent of the plant since heavy metals in the influent were mostly concentrated in dewatered sludge.

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Analytical Studies on the Rice Yield Component and Yield in South Region of Korea I. Variation in the Rice Yield Component and Yield under the Different Transplanting Dates. (남부지방에 있어서 수도수량구성요소 및 수량해석I. 재배시기에 따른 주요형질 및 수량의 변이)

  • 김용재;김규진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.3
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    • pp.209-217
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    • 1984
  • This study was conducted to establish fundamental of rice cultivation system in the southern warm region of Korea by investigation of variation of yield component factor and yield under the different transplanting dates which was at interval of 15 days, from May 20 to July 5. In the variation of leaf age, the leaf of July 5 transplanting was less 1.1 leaves than that of May 20 transplanting in Seokwang. However in Dongjin, the leaf of May 20 transplanting was more 1.7 leaves than that of July 5. According to transplanting date delay, the shorten ratio of flag leaf was showed an increasing tendency. Period from transplanting to heading under the different transplanting in Seokwang, required about 75 days in May 20 transplanting and 68 days in June 5, 67 days in June 20, but 71 days in July 5, it was a cause of decrease in grain yield, because of increase in the period from transplanting to heading date. The maximum yield under the different transplanting gathered on May 20 transplanting in Seokwang variety, and on June 20 transplanting in Dongjin variety, and then, on the accumulated temperature 1017$\pm$24$^{\circ}C$ in Seokwang, 952$\pm$15$^{\circ}C$ in Dongjin, from 10 days before heading to 30 days after heading, it was appeared the high yield. It was showed linearly negative correlation between the No. of panicles per unit area and transplanting date (Seokwang; r=-0.6768$^{**}$, Dongjin: r=-0.5182$^{**}$). There were more differentiation of spikelets per panicle in the late transplanting in Seokwang, however in Dongjin, it was decreased in differentiation of spikelets per panicle in the early and the late transplanting.ing.

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Subjective Attitudes towards Terminal Patients of Nursing Students with Clinical Practice Experience: Application of Q Methodology (임상실습을 경험한 간호대학생의 말기환자에 대한 주관적 태도: Q방법론 적용)

  • Lee, Eun Ju;Hwang, Kyung-Hye;Cho, Ok-Hee
    • Journal of Hospice and Palliative Care
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    • v.22 no.4
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    • pp.156-165
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    • 2019
  • Purpose: This study was conducted to identify subjective attitudes towards terminal patients in nursing students who had clinical practice. The types of subjective attitude were classified by applying Q methodology. According to those types, basic reference data for the development of educational programs were provided. Methods: Thirty-four final Q samples were selected, and Q classification with a nine-point scale was performed with P samples of 43 nursing students. A key factor analysis was conducted with the collected data using the PC QUANAL program. Results: Nursing students' attitudes towards terminal patients were grouped into three types. The total variable was 49.96%. Students with Type 1 ("wish for life-sustaining medical treatment") thought that terminal patients accurately understood their medical condition and wanted to prolong their lives. Others with Type 2 ("need for service and support") believed that a multidisciplinary nursing system needs to be established to help terminal patients prepare for death. Students with Type 3 ("awareness and acceptance of death") thought that terminal patients wanted to die with dignity at a hospice unit. Conclusion: This study analyzed various types of attitude towards terminal patients, as perceived by nursing students with clinical training experience. Development of educational programs for each attitude type analyzed in this study could contribute to systematic training programs for nursing students caring for terminal patients.

A Study on the Correlation Results for Fringe Rotation and Delay Tracking of the VCS (VCS의 지연추적과 프린지 회전에 대한 상관결과 고찰)

  • Oh, Se-Jin;Yeom, Jae-Hwan;Roh, Duk-Gyoo;Oh, ChungSik;Jung, Jin-Seung;Chung, Dong-Kyu;Oyama, Tomoaki;Kawaguchi, Noriyuki;Kobayashi, Hideyuki;Kono, Yusuke;Ozeki, Kensuke;Onuki, Hirohumi
    • Journal of the Institute of Convergence Signal Processing
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    • v.13 no.4
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    • pp.220-232
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    • 2012
  • In this paper, we investigate the correlation result due to the problems of delay tracking and fringe rotation module in the VCS(VLBI Correlation Subsystem). The VCS, FX-type correlator, adopts the delay tracking and fringe rotation module in order to compensate the delay change and fringe phase of wave signal from the radio source by Doppler's effect. The phase of observed data is also compensated by means of delay tracking and fringe rotation in the correlator, but we confirmed that the phase is unstable by applying long integration period of AIPS(Astronomical Image Processing System) rather than correlator. And the delay value of observed data has the errors of several tens nanoseconds than normal case at the analysis of correlation result. In addition, we found that the phase of correlation results is not connected as the unit of FFT-segment because the initial fringe phase at the fringe rotation module is not correctly determined. In this paper, in order to solve these problems, the original direction of 90 degree phase jump is reversely modified when the bit-shift occurred at the delay tracking. And the initial fringe phase at the fringe rotation module is correctly modified by using the initial phase of observed data. In addition, the parameter calculation module was abnormally operated as designed in the fringe rotation. So, the logical program by the VCS is modified so as to calculate the parameters correctly. Through the experiments of correlation processing over the above problems, the modified proposal algorithm is adequately corrected to the data analysis results, so that the experimental results make it clear for us to operate the developed VCS hardware correlator normally.

Experimental Study of the Heat Transfer Rate of the Plate Fin-Tube Condenser for a Household Refrigerator (냉장고용 판형 핀-관 응축기의 열전달 성능에 관한 실험적 연구)

  • Son, Young-Woo;Lee, Jang-Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.8
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    • pp.4802-4808
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    • 2014
  • A plate-fin tube type heat exchanger has a lighter weight, approximately 30%, than the conventional circular-fin type condenser of household refrigerator. Because the low weight means low cost, it can have significant effects on the growth of related businesses if similar performance can be guaranteed. To check the possibility of the use of such a plate fin-tube condenser, experimental evaluations were performed in this study. Four different condensers including a conventional circular fin-tube condenser were used for the test. A well designed refrigerant supply system was used to supply similar conditions with a refrigerator, and the heat transfer rate and pressure drops of air side were measured precisely. As a result, the plate fin-tube type condensers showed a lower heat transfer rate of more than 13% than the conventional circular fin-tube type condenser, but the air side pressure drop was reduced and the heat transfer per unit weight was increased. Therefore, it shows the possibility of the use of a plate fin-tube type condenser after optimizing the air flow path and increasing the air flow to make a similar heat transfer rate.

Modeling Nutrient Uptake of Cucumber Plant Based on Electric Conductivity and Nutrient Solution Uptake in Closed Perlite Culture (순환식 펄라이트재배에서 전기전도도와 양액흡수량을 이용한 오이 양분 흡수 모델링)

  • Hyung Jin Kim;Young Hoi Woo;Wan Soon Kim;Sam Jeung Cho;Yooun Il Nam
    • Journal of Bio-Environment Control
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    • v.10 no.3
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    • pp.133-140
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    • 2001
  • This study was conducted to develop a nutrient uptake model in cucumnber (Cucumis sativus L. cv. Eunsung Backdadagi) plants for prediction of the amount of nutrients in drainage solution in a closed perlite culture system. Electrical conductivity (EC) of the nutrient solution was adjusted to 1.5, 1.8, 2.1, 2.4, and 2.7 dS. $m^{-1}$ . The amount of nutrient solution absorbed in different EC treatments was not different until the mid stage of growth. However, after the mid growth stage, a high EC treatment resulted in less solution absorption. The absorption rates of K, N $O_3$$^{[-10]}$ -N, Mg, and P increased continuously for a whole growing period in all treatments, while those of Ca decreased slightly. For S, the decrease was significant after th mid stage of growth. although the amounts of absorbed inorganic ions in different EC treatments were not significantly different at the first stage of growth, they were significantly different after the mid stage of growth and decreased slightly at the end of growth stage. Models for predicting the amounts of each inorganic ion absorbed were developed by using EC and the amount of nutrient solution absorbed per unit radiation(mg.M $J^{-1}$), which proved to be practical with a positive correlation at 1 percent probability between the developed model and practical values..

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Cell Survival and Expression of Superoxide Dismutase and Catalase Genes in Saccharomyces cerevisiae Treated with N-acetyl-L-cysteine and Ionizing Radiation (Saccharomyces cerevisiae에서 이온화 방사선과 N-acetyl-L-cysteine 처리에 따른 세포 생존과 Superoxide Dismutase와 Catalase 유전자 발현)

  • Park, Ji-Young;Baek, Dong-Won;Nili, Mohammad;Kim, Jin-Kyu
    • Korean Journal of Environmental Biology
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    • v.29 no.1
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    • pp.61-67
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    • 2011
  • N-acetyl-L-cysteine (NAC) having a thiol, a precursor for glutathione (GSH), is known as one of the antioxidants. NAC used as a radioprotector against ionizing radiation (IR)-induced injury and damage. The aim of this study was to evaluate the radioprotective effects of NAC against IR-induced cell damage in Saccharomyces cerevisiae and the antioxidative effect of NAC on transcriptional level of yeast antioxidant enzyme genes such as superoxide dismutase (SOD) and catalase. In the present study, yeast cells were pretreated with various concentrations of NAC and/or irradiated with various doses of gamma rays. The cell viability was measured by counting the cell forming unit (CFU). The quantitative real-time PCR was performed for analysis of gene expression of SOD and catalase. The viability of irradiated cells was not improved by pretreatment with NAC. Ionizing radiation with 100 Gy highly induced the gene expression of antioxidant enzymes. In the irradiated group with NAC pretreatment, the gene expression of SOD and catalase was gradually reduced with the increased concentrations of NAC. These results indicate that NAC can act as a useful antioxidant to scavenge reactive oxygen species in vivo, but does not protect cells against IR-induced cell death in S. cerevisiae.

Characteristics of Marine Debris collected from the Coastline of Sandbar in the Nakdong River Estuary (낙동강 하구 사주 해안에서 수거한 쓰레기 종류 및 분포량 추정)

  • Lee, Y.B.;Park, S.;Ryu, C.R.;Kim, H.T.;Yoon, H.S.
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.10 no.3
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    • pp.148-154
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    • 2007
  • This study investigated the spatial distribution of the marine debris in Jinudo in the Nakdong river estuary. Types of marine debris in Jinudo are analyzed through sampling around some portion of its sandbar shoreline. The present study and its main results are summarized as follow: 1) Monitoring system, for evaluating the volume of marine debris, was established in the coastal region of $50\;m\;{\times}\;50\;m\;(=0.002\;km^2)$ over the southern part of Jinudo. All the marine debris of conceivable form are collected and their amount was also estimated. 2) During the $1^{st}$ collection in May 2007, the total weight of the marine debris was 1,110 kg in this site, which means the density of marine debris around the shoreline is $444\;ton/km^2$. After one month later, the collected marine debris was 23.75 kg and so we have the average density of $9.5\;ton/km^2$. From these results, it is suggested that the marine debris of 316.67kg was being accumulated per day over unit area($1\;km^2$). 3) The most frequent marine debris collected during monitoring campaigns was wooden material amounting to 85.86% of the whole collection; the rest were fishing gears such as nets and buoys(5.13%), household waste(2.34%), glass(0.94%) and metals(0.27%).

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Optimum N Topdressing for Tillering in Ridge Direct Seeding on Dry Paddy of Rice as an Irrigation Water-Saving Cultural System (벼 휴립건답직파 절수재배에 알맞은 질소 분얼비 시비량)

  • 최원영;김상수;송동석;최선영
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.3 no.2
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    • pp.75-80
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    • 2001
  • This study was conducted to identify the optimum proper nitrogen (N) application rate for tillering in ridge direct seeding on dry paddy of rice culture. During 1998, a series of experiments was carried out at paddy field (Chonbuk series) of the National Honam Agricultural Experiment Station, RDA using Dongjinbyeo. Leaf area index and top dry weight increased with higher N levels, especially in the 100 kg/ha seeding rate compared with the 60 kg/ha seeding rate. N use efficiency increased at increased by 50~75% of the N topdressing rate at tillering. Heading date, ripened grain rate, and 1,000-grain weight of brown rice did not vary among the rate of topdressing for tillering and seeding rates. The highest grain number per unit area was at the level of increased by 75% in N topdressing at tillering stage. Milled rice yield increased 5% at the 60 kg/ha seeding rate and increased 16~17% at the 100 kg/ha seeding rate at increased by 50~75% of topdressing N compared with 48 kg/ha topdressed N of 60 kg/ha seeding rate.

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