• Journal of Microbiology and Biotechnology
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• v.8 no.1
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• pp.42-48
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• 1998

In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GALl0, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.

• 한국정보디스플레이학회:학술대회논문집
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• 2004.08a
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• pp.1053-1056
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• 2004

Chunghwa Picture Tubes, LTD. (CPT) has developed a Novel TFT-LCD Driving Techniquel. This new technique is developed in combination with other state-of-the-art image processing solutions such as image compression / decompression, motion detection, and noise reduction. By applying the Novel Driving Technique to the high resolution TFT-LCD, it was found that the response time can be effectively reduced with a lower overall system cost by smaller frame memory requirement, lower EMI by less memory band-width. Likewise, higher display quality can also be achieved in that the unexpected noises generated by over-drive can be eliminated. The Novel TFT-LCD Driving Technique has been successfully implemented to the 30 inch WXGA (1280${\times}$768) resolution TFT LCD commercial TV module. It was found that the quality of moving picture was better improved compared with that of the conventional fast response driving method.

• Journal of Electrical Engineering and Technology
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• v.4 no.3
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• pp.346-352
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• 2009

The eventual goal of this paper is to help the generating companies and load-serving entities to choose appropriate relative levels of interconnected system versus bilateral trades while considering risk, and economic performance. In competitive power markets, electricity prices are determined by balance between demand and supply in electric power exchanges or bilateral contracts. The problem formulation is bilateral contract incorporated into Multi-area unit commitment with import/export and tie-line constraints. This proposed method considers maximizing own profit or minimize the operating cost among the generating companies in multi-area system. The feasibility of the proposed algorithm has been demonstrated using IEEE system with four areas and experimental results shows that proposed method is reliable, fast and computationally efficient

• Journal of the Korean Institute of Telematics and Electronics B
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• v.30B no.5
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• pp.43-51
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• 1993

A new Hangul code called Truecode is developed for accomodating to the future computing environments of graphical user interface and multimedia as well as for corresponding with the invention principle of Hangul. Truecode is not a forced two-byte code of syllable unit, as completion-type of combination-type, currently used, but a one byte code of phoneme unit, which can represent initial consonant, vowel, and final consonant each. It is quite different from three-byte code of syllable unit and also does not require the fill code used for three-byte code. We expect great contribution to the Hangul culture from Truecode's some important following features. It can express all the Korean characters we may imagine and does not cause any problem in communication. As well as we may use direct connection font, we can assign ont-to-one correspondence between Truecode and a keyboard with three sets. Truecode has a good advantage in developing application softwares of Hangul and it can nicely be applied to the fields of speech recognition and artificial intelligence using natural language.

• Carbon letters
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• v.5 no.1
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• pp.6-11
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• 2004

In spite of unparalleled combination of essential material properties for brake linings and clutch facings, replacement for asbestos is seriously called for since it is a health hazard. Once asbestos is replaced with other material then composition and properties of brake pad changes. In certain cases hardness of the material may be high enough to affect the rotor material. In this study, hardness of the brake pad has been controlled using suitable reinforcement materials like glass, carbon and Kevlar pulp. Brake pad formulations were made using CNSL (cashew net shell liquid) modified phenolic resin as a binder, graphite or cashew dust as a friction modifier and barium sulphate, talc and wollastonite as fillers. Influence of each component on the hardness value has been studied and a proper formulation has been arrived at to obtain hardness values around 35 on Scleroscopic scale. Friction and wear properties of the respective brake pad materials have been measured on a dynamometer and their performance was evaluated.

• Proceedings of the KIEE Conference
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• 2006.10d
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• pp.71-73
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• 2006

AC loss on variation of external magnetic field is a very important factor in development of power applications. In this paper, we measured and compared AC loss of hybrid-multi stacked wire made of the combination of 1G wires and 2G wires and uniform-multi-stacked wire made of one type of wires, 1G wires or 2G wires. Measurement was performed using by the linked-picked coil method. As results, as the number of wires increase, AC loss per unit volume of both stacked wires in low external magnetic field is reduced. Also AC loss of 2G slacked wire is higher than that of 1G wire. AC loss per unit length of 2G stacked wire is less than that of 1G stacked wire. And AC loss of hybrid-multi stacked wire made of the combination of 1G wires and 2G wires was between uniform-multi-stacked wire made of 1G wires and 2G wires.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.31 no.12
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• pp.1033-1041
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• 2007

This paper studied the characteristics of performance and temperature in a unit cell of a planar type SOFC under various conditions by employing computational fluid dynamics (CFD). In order to derive thermal stress distribution and performance characteristics, the 3-D model simulation for a single channel was performed in various conditions which include interconnect materials $(LaCrO_3/AISI430)$, gas flow direction (co-flow/counter-flow) and inlet temperature (923 K/1173 K). From these results of a single channel, the most effective conditions were applied to the unit stack with multi-channel and the temperature distribution is displayed. Considering both thermal stress and performance, the best combination is 923 K inlet temperature, counter-flow and interconnector of stainless steel. As the end results, flow, thermal and current density distributions were found in the model with multi-channel applied to the best combination and were concentrated in the middle of channels than in the edge.

• Korean Journal of Organic Agriculture
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• v.26 no.1
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• pp.57-72
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• 2018

Organic agriculture seeks sustainable agriculture. Organic agriculture is based on circulating agriculture of a family farm unit. However, as of the end of 2016, only 33 out of the total organic farming farms were implementing Crop-Livestock cycling organic farming. The reason seems to be a matter of income after all. The optimal size combination refers to the scale by which family farms can maintain their quality of life while engaging in farming activities. In other words. it is a farm scale that maintains optimal income through stable labor costs. In the meantime, there has been no previous study on the optimal economical combination of Crop-Livestock cycling farming. Choi (2016) analyzed whether the economies of scope (EOS) were realized in the combined production by using the management data of the farmers who practiced Crop-Livestock cycling organic farming for four years. As a result, it has been revealed that the EOS measurement value is 0 or more so the economies of scope are being realized. Therefore, the purpose of this empirical analysis is to identify farm incomes under this circumstance. It is assumed that the optimum production is achieved by balancing the total income curve and the total cost curve in the optimal scale production range. The results of the analysis are as follows. First, the income after the conversion to Crop-Livestock cycling farming was 44,789,280 won, the sum of the seedling-livestock sector, which was 17,873,120 won higher when the non-Crop-Livestock cycling farming was assumed. The same is true for 2014 and 2015. The reason for this is that pig droppings were composted from organic seedlings, and the cost of selling pork was 150,000 won/per pig more expensive even though the manufacturing cost of organic feeds was higher than the purchasing cost. Secondly, this study simulated the result that the economic index varies when the farm size combination is changed by the farm size of 100% standard (S100) as of 2014. S130 is the increase in size from 100% of 2014, whereas S30 is the result of 3ha crop and 66 livestock (pigs). As a result of this simulation, Crop-Livestock cycling farming income decreased more than non-Crop-Livestock cycling farming as the farm size decreased, whereas the income decreased as the farm size increased. When the size was reduced below S50, the income tended to decrease. In this situation, EOS changed in the same direction. The results showed that when the farming size was reorganized and reduced to 50% compared to 2014, the income and income difference was the highest. At the same time, economies of scope (EOS) were the highest at 0.12985. In other words, it was found that the income of farm houses in a family farm unit sector was the best in the combination of 1.5ha crop agriculture and 110 livestock (pigs).

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1179-1188
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• 2023

Escherichia coli, particularly multidrug-resistant (MDR) strains, is a serious cause of healthcare-associated infections. Development of novel antimicrobial agents or restoration of drug efficiency is required to treat MDR bacteria, and the use of natural products to solve this problem is promising. We investigated the antimicrobial activity of dried green coffee (DGC) beans, coffee pulp (CP), and arabica leaf (AL) crude extracts against 28 isolated MDR E. coli strains and restoration of ampicillin (AMP) efficiency with a combination test. DGC, CP, and AL extracts were effective against all 28 strains, with a minimum inhibitory concentration (MIC) of 12.5-50 mg/ml and minimum bactericidal concentration of 25-100 mg/ml. The CP-AMP combination was more effective than CP or AMP alone, with a fractional inhibitory concentration index value of 0.01. In the combination, the MIC of CP was 0.2 mg/ml (compared to 25 mg/ml of CP alone) and that of AMP was 0.1 mg/ml (compared to 50 mg/ml of AMP alone), or a 125-fold and 500-fold reduction, respectively, against 13-drug resistant MDR E. coli strains. Time-kill kinetics showed that the bactericidal effect of the CP-AMP combination occurred within 3 h through disruption of membrane permeability and biofilm eradication, as verified by scanning electron microscopy. This is the first report indicating that CP-AMP combination therapy could be employed to treat MDR E. coli by repurposing AMP.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.12
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• pp.899-912
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• 2009

With greater utilization of web application and growth of its market, the function of such application is being expanded With today's increasing demand for high quality software, we see a sharp rise in the interest for web application test. The current trend is that web applications are being developed based on a framework of developmental environment. Hence, as the scope of that framework expands, each module of the entire web application is being configured by the combination of heterogeneous files. The distinctive characteristics of a web application are based on the fact that the framework that is provided in partially completed form controls the structure of all objects of development. The present study defines the web business logic as a minimal unit which has meaning as a unit of application for the web application. In this paper, we define web business logic as a least meaningful execution unit of web applications, propose how to generate automatically the BizUnit test code for it, and analyze the effectiveness of testing of the web application via the BizUnit test codes.