• 미술이론과 현장
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• 제13호
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• pp.185-213
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• 2012

Charles Stankievech's 2011 installation LOVELAND includes a wall-sized screen depicting video footage of a barren arctic landscape in an enclosed room, painted and bathed in white light, that appears as an extension of the imaged environment. A melodic and industrial musical score emanates from multiple sound panels and as the music increases a cloud of purple smoke becomes visible on the horizon line in the distance and gradually advances toward the viewer until it completely fills the screen. The smoke then remains, rushing about madly and lapping at the border between the screen and the room before it suddenly subsides and the spectator is again left with the desolate landscape. The entire process takes a mere five minutes and then, fixed on an endless loop, begins again. This paper positions LOVELAND as an attempt to simulate a sublime experience of the end of the world through a transposition of the Arctic atmosphere into the gallery space. Encompassing a discussion of the historical and contemporary significance of the Arctic in popular culture, aesthetics and environmental politics, it is suggested that Stankievech employs an apocalyptic trope in reference to the unstable position of the North in the current political and ecological climate. Revisiting critiques of modernist exhibition practices and investigating the perceptual and temporal dimensions of the work, this analysis focuses primarily on the experience of the installation's spectator. Visually, aurally and phenomenologically immersed, the viewer is made subject to, and implicated in, the events unfolding on the screen and within the space. Due to the looping of the video footage, this paper argues that the apocalypse imaged in LOVELAND is presented as an endless event - incessantly enacted, yet infinitely deferred - and that the spectator is enveloped in an uncertain and unceasingly extended present moment.

• 대한한의학원전학회지
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• 제22권3호
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• pp.161-185
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• 2009

This article looks into Lee Gyujun(李圭晙)'s life course, Confucian thoughts, and philosophical ideas on the Medicine of Boosting Yang by translating selectively his "Seokgoksango(石谷散稿)". Translating his works is a pre-requisite in order to fully understand the significance of boosting-yang theory in the historical landscape of Korean medicine, not just to comprehend the Medicine of Boosting Yang. First, it is found through the translation that a previous version of "Seokgoksango" had been probably printed before its existing version 1981. Secondly, there are numerous resistants to the Japanese ruling of Korea among his friendship. Thirdly, his academical root came from the Gihohakpa(畿湖學派) - the Giho school of Korean Seongli Confucianism[朝鮮性理學]. Its genealogy in Gyeongsang(慶尙) province is as follows: Lee Yi(李珥), Song Siyeol(宋時烈), Hong Jikpil(洪直弼), Seo Changyu(徐贊奎) and Lee Gyujun(李圭晙), and last, he kept contact with the Toegyehakpa(退溪學派), the Toegye school of Korean Seongli Confucianism, even though his academic root was closely linked to the Giho school. If the entire parts of "Seokgoksango" is translated, it will serve as invaluable historical document to understand a medical unfolding around the 20th century in Korea.

• 한국자기공명학회논문지
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• 제19권3호
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• pp.99-105
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• 2015

Hsp33 is a prokaryotic molecular chaperon that exerts a holdase activity upon response to an oxidative stress at raised temperature. In particular, intramolecular disulfide bond formation between the four conserved cysteines that bind a zinc ion in reduced state is known to be critically associated with the redox sensing. Here we report the backbone NMR assignment results of the half-oxidized Hsp33, where only two of the four cysteines form an intramolecular disulfide bond. Almost all of the resolved peaks could be unambiguously assigned, although the total assignments extent reached just about 50%. Majority of the missing assignments could be attributed to a significant spectral collapse, largely due to the oxidation-induced unfolding of the C-terminal redox-switch domain. These results support two previous suggestions: conformational change in the first oxidation step is localized mainly in the C-terminal zinc-binding domain, and the half-oxidized form would be still inactive. However, some additional regions appeared to be potentially changed from the reduced state, which suggest that the half-oxidized conformation would be an intermediate state that is more labile to heat and/or further oxidation.

• BMB Reports
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• 제43권3호
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• pp.176-181
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• 2010

The primary sigma factor ($\sigma^{A}$) of Staphylococcus aureus, a potential drug target, was little investigated at the structural level. Using an N-terminal histidine-tagged $\sigma^{A}$ (His-$\sigma^{A}$), here we have demonstrated that it exits as a monomer in solution, possesses multiple domains, harbors primarily $\alpha$-helix and efficiently binds to a S. aureus promoter DNA in the presence of core RNA polymerase. While both N- and C-terminal ends of His-$\sigma^{A}$ are flexible in nature, two Trp residues in its DNA binding region are buried. Upon increasing the incubation temperature from 25$^{\circ}$ to 40$^{\circ}C$, $\sim$60% of the input His-$\sigma^{A}$ was cleaved by thermolysin. Aggregation of His-$\sigma^{A}$ was also initiated rapidly at 45$^{\circ}C$. From the equilibrium unfolding experiment, the Gibbs free energy of stabilization of His-$\sigma^{A}$ was estimated to be +0.70 kcal $mol^{-1}$. The data together suggest that primary sigma factor of S. aureus is an unstable protein. Core RNA polymerase however stabilized $\sigma^{A}$ appreciably.

• Korean Chemical Engineering Research
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• 제44권1호
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• pp.92-96
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• 2006

유기용매에서 효소를 이용하면 다양한 선택적 반응을 쉽게 수행할 수 있어 산업적 적용 가능성이 매우 높지만, 효소의 안정성 저하는 아직까지 큰 문제 중의 하나로 남아있다. 유기용매에서 효소 반응시 효소의 실활 원인과 효소의 안정화 방법 연구를 위하여 단백질의 folding에 관여하는 molecular chaperone의 일종인 heat-shock protein Hsp90을 이용하여, 대표적인 유기용매 반응시스템에서의 과산화효소 HRP 안정성 향상 연구를 수행하였다. 그 결과 Hsp90은 30％ DMSO, 30％ 및 50％ dioxane 완충용액에서 HRP의 실활 방지 효과를 보였고, 실활된 효소의 재생에도 탁월한 효과를 보였다. 그리고 형광분석과 CD(circular dichroism)에 의한 구조분석을 수행하여 Hsp90이 유기용매에 의해 unfolding되어 있는 효소를 다시 refolding하는 데 기여함을 알았다.

• 한국정밀공학회지
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• 제15권3호
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• pp.18-23
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• 1998

This paper describes a research work of developing a computer-aided design of blanking and piercing for irregular-shaped sheet metal products. An approach to the CAD system is based on the knowledge-based rules. Knowledge for the CAD system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in AutoLISP on the AutoCAD with a personal computer and is composed of four main modules, which are input and shape treatment, flat pattern layout, production feasibility check, and strip layout module. Based on knowledge-based rules, the system is designed by considering several factors, such as radius and angle of bend. material and thickness of product, complexities of blank geometry and punch profile, and availability of press. This system is capable of unfolding a formed sheet metal part to give flat pattern and automatically account for the adjustment of bend allowances to match tooling requirements by checking the dimensions and relationships of parts of the folded product. Also this system can carry out a process planning which is obtained from results of irregular shape of product that was successful in production feasibility check module according to flat pattern layout and generate strip layout drawing in graphic forms. The developed system provides its efficiency for flat pattern layout, and strip layout for the irregularly shaped sheet metal products.

• 한국정밀공학회지
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• 제15권6호
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• pp.22-32
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• 1998

This paper describes a research work of developing a computer-aided design of irregularly shaped-sheet metal product with bending and piercing operation for progressive working. An approach to the CAD system is based on the knowledge-based rules. Knowledge for the CAD system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in AutoLISP on the AutoCAD with a personal computer and is composed of five main modules, which are input and shape treatment, flat pattern-layout, production feasibility check, blank-layout, and strip-layout module. Based on knowledge-based rules, the system is designed by considering several factors, such as radius and angle of bend, material and thickness of product, complexities of blank geometry and punch profile, and availability of press. This system is capable of unfolding a formed sheet metal part to give flat pattern and automatically account for the adjustment of bend allowances to match tooling requirements by checking dimensions and the best utilization ratio of blank-layout within bending production feasibility area which is beyond ${\pm}30^{\circ}$ degrees intersecting angle between grain flow and bending edge line and which is suitable to progressive bending operation. Also the strip-layout drawing generated by a bending and a piercing operation according to punch profiles divided into automatically for external area of irregularly shaped-sheet metal product is displayed in graphic forms.

• 한국작물학회지
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• 제34권1호
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• pp.98-105
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• 1989

소맥생육기간중의 GS활성도의 경시적 변이진상을 알고저 소맥품종 Kolibri의 시비수준별, 엽위별 활성도를 분석한 결과, 1. 각엽에 있어서 생체종 1g당 GS활성도는 엽분화 초기에 극히 낮으나 엽의 전개, 신장에 따라 수배까지 증가하며 엽의 노화 진전과 더불어 하락하였다. 2. 생체종 1g당 GS활성도는 엽의 분화초기를 제외하면 아래로부터 상위엽 차순으로 높았으며, 최고의 활성도는 지엽에서 나타났다. 3. 최상위 2개엽은 최하위엽(제 1-4엽)보다 활성도와 활성 peak가 전 처리구에서 모두 2-3배 높았다. 4. 생체종 1g당 GS활성도는 제 1-4엽에서 1회의 활성 peak를 보인 반면 제6-지엽에서는 2회의 peak를 나타냈다. 5. GS활성도는 출tnrlRK지 증가하여 첫 번째 활성 peak에 이르고 그 이후 개화후 7일의 유숙기까지 급락하였다가 다시 증가하여 개화후 22일인 호숙기에 두 번찌 peak에 이르는 V자형 함몰현상을 나타냈다.

• 생명과학회지
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• 제14권2호
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• pp.309-314
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• 2004

cAMP수용성 단백질(CRP)은 E. coli의 100가지 이상의 유전자 전자조절에 관계된다. CRP는 dimer로 존재하며 cAMP의 결합으로 활성인 형태로 전환된다. 이중체인 CRP 단백질의 열 안정성과 구조 전이의 연구에 효과적인 온도 기울기 전기영동장치를 이용하여 확인하였다. 본 연구에서 야생형과 S83C CRP 단백질의 melting temperature (Tm)는 산성인 완충용액[89.8 mM Glycine, 24mM Boric acid (pH 5.8)]에서 57$\pm$1(야생형 CRP)과 55$\pm$1$^{\circ}C$ (S83G CRP)였다. 그리고 온도에 따른 CRP 단백질의 구조변화도 protease digestion과 CD spectropolarimeter을 이용하여 확인하였다.

• Journal of Radiation Protection and Research
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• 제20권2호
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• pp.129-136
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• 1995

중저준위 에너지 범위에서 방사선관리용측정기기의 에너지의존성에 대한 연구를 위하여 ISO에서 는 ISO표준 X선장을 발간하였으며, 또한 ANSI에서도 개인방사선측정 기기의 성능시험을 위하여 ANSI X선장을 제시 하였다. 그러나 X선 스펙트럼의 측정은 많은 시간이 소요될 뿐만 아니라 특히 측정 X선 스펙트럼에 unfolding이 수행된다면 매우 어려운 작업이 된다. 따라서 이론적인 방법에 의해 스펙트럼을 계산 예측하고 그 평균에너지 및 선량환산인자를 계산하는 것은 X선 실험제작에 필요한 시간, 노력 및 비용을 최소화 할 수 있을 것이다. 따라서 본 연구에서는 ISO Narrow series 및 ANSI X선장에 대하여 이론적으로 스펙트럼을 계산하였으며, 그에 의한 평균에너지와 선량당량혼산인자를 계산하였다. 본 계산에 의한 평균에너지 및 선량환산인자는 측정스펙트럼에 의한 ISO 및 X선장의 값과 잘 일치하였다.