• Journal of Institute of Control, Robotics and Systems
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• v.7 no.4
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• pp.344-350
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• 2001

In this paper, we are focused on the analysis of acyclic schedule for the determination of the opti-mal cycle time and minimization of work in process. Especially, this paper deals with the product ratio-driven cyclic scheduling problem in FMS with different products and ratio using the TPN(Time Petri Nets)un-folding.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.14 no.24
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• pp.179-185
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• 1991

This study aims to consider the necessity and meaning of the Company-Wide Creativity Development(CWCD) for the unfolding of the strategic management in the creation age. The brief summary of the meaning of the CWCD for the unfolding of the strategic management is as follows. (1) The activation of the information interchange through the consonance among the all department. (2) The Information interchange and cooperation between the worker and the manage. (3) The creativity development of worker.

• BMB Reports
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• v.39 no.1
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• pp.55-60
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• 2006

We describe a phage display strategy, based on the differential resistance of proteins to denaturant-induced unfolding, that can be used to select protein variants with improved conformational stability. To test the efficiency of this strategy, wild-type and two stable variants of ${\alpha}_1$-antitrypsin (${\alpha}_1AT$) were fused to the gene III protein of M13 phage. These phages were incubated in unfolding solution containing denaturant (urea or guanidinium chloride), and then subjected to an unfavorable refolding procedure (dialysis at $37^{\circ}C$). Once the ${\alpha}_1AT$ moiety of the fusion protein had unfolded in the unfolding solution, in which the denaturant concentration was higher than the unfolding transition midpoint ($C_m$) of the ${\alpha}_1AT$ variant, around 20% of the phage retained binding affinity to anti-${\alpha}_1AT$ antibody due to a low refolding efficiency. Moreover, this affinity reduced to less than 5% when 10 mg/mL skimmed milk (a misfolding-promoting additive) was included during the unfolding/refolding procedure. In contrast, most binding affinity (>95%) remained if the ${\alpha}_1AT$ variant was stable enough to resist unfolding. Because this selection procedure does not affect the infectivity of M13, the method is expected to be generally applicable to the high-throughput screening of stable protein variants, when activity-based screening is not possible.

• Journal of Korea Multimedia Society
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• v.9 no.9
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• pp.1160-1172
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• 2006

Unfolding is an efficient technique to detect colorectal disease since it represents entire shape of colon at a glance. However, the previous unfolding methods may miss the surface located behind the folds or high curvature areas. To solve the problem, we propose an adaptive unfolding method that controls the number of rays in accordance with the surface complexity. In the first step, we find sample points of longer and shorter distance between the sample points and the colon surface, and then determine them as based control points. Next, for reaching rays of sample points in the wrinkled colon surface, sample points are shifted toward the control points that have the long distance. Lastly, we perform a 2D scaling which solves local distortions of the unfolding surface and avoids missing surface. Consequently, our method could be visualized the hidden surface of colon organ in detail.

• BMB Reports
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• v.35 no.2
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• pp.143-154
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• 2002

The structural and functional aspects of ervatamin B were studied in solution. Ervatamin B belongs to the $\alpha+\beta$ class of proteins. The intrinsic fluorescence emission maximum of the enzyme was at 350 nm under neutral conditions, and at 355 nm under denaturing conditions. Between pH 1.0-2.5 the enzyme exists in a partially unfolded state with minimum or no tertiary structure, and no proteolytic activity. At still lower pH, the enzyme regains substantial secondary structure, which is predominantly $\beta$-sheet conformation and shows a strong binding to 8-anilino-1-napthalene-sulfonic acid (ANS). In the presence of salt, the enzyme attains a similar state directly from the native state. Under neutral conditions, the enzyme was stable in urea, while the guanidine hydrochloride (GuHCl) induced equilibrium unfolding was cooperative. The GuHCl induced unfolding transition curves at pH 3.0 and 4.0 were non-coincidental, indicating the presence of intermediates in the unfolding pathway. This was substantiated by strong ANS binding that was observed at low concentrations of GuHCl at both pH 3.0 and 4.0. The urea induced transition curves at pH 3.0 were, however, coincidental, but non-cooperative. This indicates that the different structural units of the enzyme unfold in steps through intermediates. This observation is further supported by two emission maxima in ANS binding assay during urea denaturation. Hence, denaturant induced equilibrium unfolding pathway of ervatamin B, which differs from the acid induced unfolding pathway, is not a simple two-state transition but involves intermediates which probably accumulate at different stages of protein folding and hence adds a new dimension to the unfolding pathway of plant proteases of the papain superfamily.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.3
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• pp.283-292
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• 2009

Considering an integral equation governing the motion of unfolding fin, an algebraic equation was acquired to get estimated minimum deployment energy required for the successful fin unfolding under the given wind condition. To complete the integration of moment, some approximations had to be introduced particularly to frictional moment and aerodynamic damping for which deployment angular speed of the unfolding fin was modelled as a function of deployment angle only with assumed profile using expected maximum angular speed. Technique for the estimation of the minimum required deployment energy was finalized by introducing the ideal deployment angular speed representing work done by the fin unfolding device alone during fin unfolding and was confirmed by comparing results from simulation with various aerodynamic conditions and profiles of the hinge torque.

• Nuclear Engineering and Technology
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• v.55 no.3
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• pp.1021-1030
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• 2023

We propose an overall procedure for measuring and unfolding fast neutron spectra using a trans-stilbene scintillation detector. Detector characterization was described, including the information on energy calibration, detector resolution, and nonproportionality response. The digital charge comparison method was used for the investigation of neutron-gamma Pulse Shape Discrimination (PSD). A pair of values of 600 ns pulse width and 24 ns delay time was found as the optimized conditions for PSD. A fitting technique was introduced to increase the trans-stilbene Proton Response Function (PRF) by 28% based on comparison of the simulated and experimental electron-equivalent distributions by the Cf-252 source. The detector response matrix was constructed by Monte-Carlo simulation and the spectrum unfolding was implemented using the iterative Bayesian method. The unfolding of simulated and measured spectra of Cf-252 and AmBe neutron sources indicates reliable, stable and no-bias results. The unfolding technique was also validated by the measured cosmic-ray induced neutron flux. Our approach is promising for fast neutron detection and spectroscopy.

• Korean Institute of Interior Design Journal
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• v.21 no.4
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• pp.68-76
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• 2012

Contemporary architecture has a tendency to concretely approach based on the concept of Deuleuze's 'fold' diversity problems creating continuously potential diversities within Post-structural paradigm. Contemporary architecture relates to human life through its relation with human being. The research investigates through case study the possible relation of the concept of Deulezue's 'fold', one of his concepts of 'unfolding' based on Monadology, with logic of creation that constructs a space in contemporary architectural space. Eight contemporary architectural spaces since 1990 with architectural embodiment of Deulezue's 'fold' concept have been selected as cases. Based on the fact that the potential possibility of a space can be embodied by the thinking of 'fold', the research draws four physical activities such as 'properties connection', 'beyond border', 'diversity of circulation' and 'potential opportunity', through which it studies on the principle of actualization of space creation. Consideration of metaphysical theory as a pre-modern and outdated one may lose a huge potentiality. Leibniz's metaphysics can be rediscovered as a model that provides diverse potentiality. The concept of 'fold' in Monadology needs be reconsidered because it possibly suggests a methodology that embodies through the actualization of space the meanings underlain in contemporary architectural space.

• BMB Reports
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• v.40 no.2
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• pp.205-211
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• 2007

The stability curve - a plot of the Gibbs free energy of unfolding versus temperature - is calculated for bovine erythrocyte carbonic anhydrase in 150 mM sodium phosphate (pH = 7.0) from a combination of reversible differential scanning calorimetry measurements and isothermal guanidine hydrochloride titrations. The enzyme possesses two stable folded conformers with the conformational transition occurring at ~30$^{\circ}C$. The methodology yields a stability curve for the complete unfolding of the enzyme below this temperature but only the partial unfolding, to the molten globule state, above it. The transition state thermodynamics for the low- to physiological-temperature conformational change are calculated from slow-scan-rate differential scanning calorimetry measurements where it is found that the free energy barrier for the conversion is 90 kJ/mole and the transition state possesses a substantial unfolding quality. The data therefore suggest that the x-ray structure may differ considerably from the physiological structure and that the two conformers are not readily interconverted.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.5
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• pp.420-427
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• 2008

For simulation of a fin unfolding motion for the various aerodynamic conditions, equations and moments applying to the unfolding fin were modelled. Aerodynamic roll moment consists of the static roll moment and the damping moment, which were obtained through wind tunnel tests and numerical analyses respectively. Panel method was used to compute the roll damping coefficient with deflected fin, whose angle was equivalent to angle of attack due to the deployment motion. Roll damping coefficient is a function of angle of attack, sideslip angle, and deployment angle but not of angular velocity of deployment. Simulation with aerodynamic damping model gave more similar deployment time compared to fin deployment test results.