• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.402-406
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• 2005

Lipomyces starkeyi produces a novel glucanhydrolase containing endo-dextranase and ${\alpha}-amylase$ activities. A cDNA from L. starkeyi encoding a dextranase was isolated and characterized. The 2,052 kb cDNA fragment (lsd1) carrying dextranase gene showed one open reading frame (ORF) composed of 1,824 bp flanked by a 41 bp 5'-UTR and a 184 bp 3'-UTR including a poly(A) tail of 27 bp. The ORF encodes for a 608 amino acid with a predicted molecular mass of 67.6 kDa. There was 77% deduced amino acid sequence identity between the LSD1 dextranase and the dextranase from Penicillium minioluteum. The primary structure of the dextranase from L. starkeyi has distant similarity with enzymes belonging to glycosyl hydrolase family 49. The lsd1 protein was expressed in the Saccharmyces cerevisiae under control of GAL1 promoter and active dextranase was produced.

• Journal of The Korean Association For Science Education
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• v.17 no.2
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• pp.127-137
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• 1997

The purpose of this study was to assess elementary student's ability of measuring length, area, and volume and reading metric scales. The findings of this study were as follows. All students were successful in measuring without scale, regardless of grades. But they were less successful in measuring the task using standard and nonstandard scales. Especially, in measuring area, lower grade students were not able to perform the task using nonstandard instruments, but higher grade students were able to perform the task. Measuring area using grade paper was least successful for sixth graders. In measuring volume, students under fifth grade read the upper scale when using graduated cylinder. In measuring by direct comparison, students were more successful in measuring length than in measuring area and volume using given material. The use of given nonstandard material was successful for 4th, 5th, and 6th grade students. In measuring using metric scale, lower grade students did not pay attention to the zero of the scale, but just read the end point. Higher grade students considered both zero and end points.

• Journal of Microbiology and Biotechnology
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• v.10 no.6
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• pp.823-828
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• 2000

The complete nucleotide sequence of hepatitis B virus DNA isolated from Korean patient serum was determined and characterized, and its phylogenetic relation was then investigated. The viral genome was 3,215 base pairs long and included four well known open reading frames (i.e. surface antigens, core antigens, X protein and DNA polymerase). The sequence of the surface antigen showed that the HBV genome under investigation, designated HBV 315, was characteristic of subtype adr. A phylogenetic analysis using the total genome sequence revealed that HBV315 was grouped into genomic group C together with isolates from Japan, China, Thailand, Polynesia, and New Caledonia. The mean percent similarity between HBV315 and other HBV isolates in genomic group C was 97.25%, and that with other genomic groups ranged from 86.16% to 91.25%. The predicted amino acid sequences of HBV315 were compared with two closely related subtype adr isolates, M38636 and D12980. The results showed that the X gene product was identical in the three strains, while there were significant amino acid sequence differences between HBV315 and M38636 in the Pre-S1 and Pre-S2 regions.

• Journal of the Korean Society for Library and Information Science
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• v.4
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• pp.1-26
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• 1975

The present study is a literature survey aimed at . providing a means to improve the bibliographic apparatuses for Korean studies. The need for a study like this has proven urgent with the promotion of Korean studies both within and without in recent years. The study examines the social science literature on Korea produced in English between 1876 and 1945(A study is under way for literature since 1945 also). Selection of the titles for investigation was made on the basis of the relative importance of the works confirmed after reading book reviews and examining a variety of bibliographies. Each title selected was examined in terms of its subject matter, contribution to the discipline it belongs, references cited, relations with similar works in the field, and bibliographic features. Published books, dissertations both published or not published, and periodicals are included. Primarily a critical review it is also designed to provide background information on the present status of Korean studies and the historical development of Western studies on Korea. Special attenttion is given to the factors contributing to the foundations of the modern Western scholarchip on Korea in the late 19th century.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.4
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• pp.286-290
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• 2005

Ascorbate peroxidase (APX) plays a crucial role in the detoxification of hydrogen peroxide. APX activity is maintained significantly higher in the paraquat­treated leaves of the paraquat-tolerant Rehmannia glutinos. This study was conducted to understand structural and regulatory characteristics of APX gene in R. glutinosa. A putative APX cDNA clone (RgAPX1) was isolated from a leaf cDNA library using a partially sequenced expressed sequence tag clone. RgAPX1 is consisted of 1148 bp nucleotides and contains an open reading frame encoding a 250 amino acid-long polypeptide. Deduced RgAPX1 amino acid sequence shares higher sequence similarity to cytosolic APXs. RgAPX1. expression was higher in the leaf than in the flower and root. Southern blot result indicates the presence of one or two RgAPX1-related genes in R. glutinosa genome. RgAPX1 transcription was affected differentially by various stresses and phytohormone. The results indicate that RgAPXl is constitutively expressed in most tissues and its expression is modulated for the immediate and efficient detoxification of $H_2O_2$ under normal and stress conditions.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1327-1332
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• 2004

The hemA gene encoding 5-aminolevulinic acid synthase (ALAS) was cloned from Rhodobacter capsulatus, and its nucleotide sequence was determined. DNA sequencing data revealed one open reading frame coding for a protein with 401 amino acids that displayed high similarity to the amino acid sequences of other known ALASs. The hemA gene was then cloned and expressed under the control of constitutive promotor in E. coli. The recombinant E. coli strain was able to accumulate 5-aminolevulinic acid to 21 mM in the liquid medium supplemented with 45 mM glycine and 120 mM succinate. In addition, a marked effect of the pH of the culture medium on ALA production was observed, and the optimum pH for culture medium was determined to be 5.8-6.3.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1629-1635
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• 2016

A novel bacteriophage, PBES 02, infecting Cronobacter sakazakii was isolated and characterized. It has a spherical head of 90 nm in diameter and a tail of 130 nm in length, and belongs to Myoviridae as observed under a transmission electron microscope. The major virion protein appears to be 38 kilodaltons (kDa) in size. The latent period of PBES 02 is 30 min and the burst size is 250. Infectivity of the phage remained intact after exposure to temperatures ranging from 4℃ to 55℃ for 1 h. It was also stable after exposure to pHs ranging from 6 to 10 for 1 h. The phage effectively removed contaminating Cronobacter sakazakii from broth infant formula. PBES 02 has a double-stranded DNA genome of 149,732 bases. Its GC ratio is 50.7%. Sequence analysis revealed that PBES 02 has 299 open reading frames (ORFs) and 14 tRNA genes. Thirty-nine ORFs were annotated, including 24 related to replication and regulation functions, 10 related to structural proteins, and 5 related to DNA packaging. The genome of PBES 02 is closely related to that of two other C. sakazakii phages, CR3 and CR8. Comparison of DNA sequences of genes encoding the major capsid protein revealed a wide geographical distribution of related phages over Asia, Europe, and America.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.23
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• pp.223-247
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• 2004

United Nations Commission on International Trade Law(hereinafter"UNCITRAL"), the core legal body of the United Nations system in the field of international trade law, is currently in the process of preparing a draft instrument on the international carriage of goods. In order to facilitate and prompt for new draft instrument, Working Group Ⅲ was established under the auspicious of UNCITRAL in 2002. Working Group, which was composed of all member countries of UNCITRAL, considered the text of preliminary draft instrument on the carriage of goods by sea. According to the Working Group's report, this new convention deals with issues relating to the international ocean carriage of goods such as the scope of application, the period of responsibility of the carrier, liability of the carrier, obligations of the shipper and transport documents including electronic records. In the course of the second reading, however, there are lots of outstanding issues to deliberate and consider for formulating new version of the ocean cargo liability convention. One of the substantial issues is the snail's pace of progress in last sessions. Therefore legal adviser and industry representatives assume the next UNCITRAL meeting, in Vienna, Nov. 29-Dec. 10, would be more critical to complete the convention.

• Journal of Microbiology and Biotechnology
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• v.13 no.1
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• pp.139-145
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• 2003

The gene encoding Thermus caldophilus GK24 polyphosphate kinase (Tca PPK) was cloned and sequenced. The gene contains an open reading frame encoding 608 amino acids with a calculated molecular mass of 69,850 Da. The deduced amino acid sequence of Tca PPK showed a 40% homology to Escherichia coli PPK, and $39\%$ to Klebsiella aerogenes PPK. The Tca ppk gene was expressed under the control of the T7lac promoter on pET-22b(+) in E. coli and its enzyme was purified about 70-fold with $36\%$ yield, following heating and HiTrap chelating HP column chromatography. The native enzyme was found to have an approximate molecular mass of 580,000 Da and consisted of eight subunits. The optimum pH and temperature of the enzyme were 5.5 and $70^{\circ}C$, respectively. A divalent cation was required for the enzyme activity, with $Mg^2+$ being the most effective.

• Architectural research
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• v.20 no.2
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• pp.35-43
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• 2018

This article explores the complex modes of experiencing the modern city that are engaging and disengaging by nature, which thus negates any simple ways of understanding what it means by 'the urban' in a Manichean comparison. What follows is an in-depth case study of Gordon Matta-Clark's 1976 film titled City Slivers. Influenced by the countercultural practices prevalent in the 1960s and 1970s, Matta-Clark produced a number of works roughly grouped together under the rubric of "building cuts." Among many others, City Slivers is distinctive among Matta-Clark's extensive cutting projects, in the sense that he actively utilizes film as a primary expressive medium and poetically reassembles fragmentary images of cityscape in order to bring forth an alternative urban scenario where the tension between institution-bound urbanization and dispersed daily urban practices is highlighted. Instead of simply being critical against the changing urban conditions of Manhattan in the 1970s, Matta-Clark aims to actively grasp ambivalent instances of urban life that are at once attractive and alienating, thereby excavating the subconscious terrain of contemporary urbanism that is prevalent but often dismissed over glamorous urban projects.