• 대한한의학회지
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• 제28권4호
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• pp.158-167
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• 2007

Background & Objective : Uncaria rhynchophylla is traditional medicine herb used for enhancing body resistance against various diseases. The aim of this study was to identify if Uncaria rhynchophylla extracts induce osteogenic activity in human osteoblast-like SaOS-2 cells. Methods : The osteogenic activity of Uncaria rhynchophylla was evaluated on cell proliferation assay by WST-8, and osteoblast-specific genes, such as VEGF, type I collagen (Col I), osteocalcin (OCN), and osteopontin (OPN) by RT-PCR analysis and ELISA assay in osteoblasts-like SaOS-2 cells. Bone mineralization was stained with Alizalin red method. Results : Uncaria rhynchophylla had significantly increased cell proliferation at a dose dependent manner in human osteoblast-like SaOS-2 cells. Uncaria rhynchophylla markedly increased alkaline phosphatase (ALP), vascular endothelial growth factor (VEGF) mRNA expression at 7 days and dose dependently increased ALP activity and VEGF secretion in human osteoblast-like SaOS-2 cells. Also, Uncaria rhynchophylla time-dependently increased type I collagen (Col I), osteopontin (OPN), and osteocalcin (OCN) mRNA in SaOS-2 cells. Extracellular accumulation of proteins such as Col I and OCN was maximal increased by Uncaria rhynchophylla at 10 ${\mu}g/ml$. Also, Uncaria rhynchophylla significantly induced mineralization in the culture of SaOS-2 cells. Conclusion : This study showed that Uncaria rhynchophylla had enhanced proliferation, ALP activity, VEGF, bone matrix proteins such as OCN, OPN, and Col I, and mineralization in SaOS-2 cells. These results propose that Uncaria rhynchophylla can play an important role in osteoblastic bone formation, osteogenesis, and may possibly lead to the development of bone-forming drugs.

• Natural Product Sciences
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• 제11권3호
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• pp.145-149
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• 2005

A methanol soluble extract from the dried hooks and stems of Uncaria rhynchophylla showed a strong inhibitory activity against monoamine oxidase in mouse brain. Using a bioassay-guided purification of this extract, a known ${\beta}-carboline$ type alkaloid, harman (1), was obtained as an active constituent. In addition, five known indole alkaloids, isocorynoxeine (2), isorhynchophylline (3), corynoxeine (4), cadambine (5), and $3{\alpha}-dihydrocadambine$ (6), were isolated and found to be weakly active or inactive.

• Applied Biological Chemistry
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• 제43권1호
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• pp.78-80
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• 2000

• 생명과학회지
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• 제30권12호
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• pp.1033-1041
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• 2020

최근 모든 연령대의 사람들은 미용적인 이유로 더 밝은 피부를 원하고 있으며, 천연 제품은 화학적으로 합성된 화합물보다 더 많은 관심을 받고 있다. 조구등은 아시아에서 전통 한약재로 널리 사용되어 왔다. 새로운 피부 미백제를 찾기 위해 본 연구에서는 조구등의 항산화 활성과 잠재적인 tyrosinase 억제 작용을 확인하였다. 조구등을 70% 에탄올로 추출하여 항산화 활성을 분석하고 tyrosinase 활성과 melanin 합성에 미치는 영향을 평가했다. 총 mRNA 발현은 RT-PCR을 사용하였다. 그 결과 조구등 추출물은 B16F10 세포에서 뛰어난 항산화 능력과 상당한 수준의 폴리페놀 및 플라보노이드 화합물을 함유하였다. 또한, 세포 내 tyrosinase 활성을 억제하고 처리된 세포에서 melanin의 양을 감소시켰다. Tyrosinase의 mRNA 발현은 1 mg/ml 농도에서 현저히 감소하였다. 이와 같은 결과는 조구등이 미백 효과가 있는 화장품의 천연 소재로 사용될 수 있는 높은 잠재력을 가지고 있음을 시사한다.

• 대한한의학회지
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• 제20권3호
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• pp.66-76
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• 1999

The purpose of this study was to asses the etlect of Uncaria rhynchophylla (UR). one of the oriental herbs having a capability to block calcium channels, on affecting vestibular compensation (VC) in Sprague - Dawley rats. Animals were divided into a drug treatment group receiving, UR per oral for 10 days preceding unilateral labyrinthectomy (ULX) and a control group with saline ingestion, To evaluate behavioral changes. horizontal spontaneous nystagmus (SN) and roll head tilt (RHT) were recorded by a video camem with zoom lens in the course of vestibular compensation (VC). Immunohistochemical staining was performed by conventional ABC method to visualize cFos-like immunoreactive (cFLI) neurons in the medial vestibular nuclei (MVN) and cFLI cells were counted by image analyzer. Bodyweight was increased significantly, about 35g, by UR treatment for 10 clays before ULX. Compared with the control group, the drug group showed significant reduction of RHT 6 hrs after ULX as well as fast disappearance of SN at early stages of vestibular compensation. Also, recovery of the spatial and temporal cFLI expressions in the bilateral MVN was accelerated 24 hrs after ULX. These results suggest that Uncaria rhynchophyila has a beneficial effect to ameliorate vestibular compensation in unilateral labryinthectomized rats.

• 대한의생명과학회지
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• 제27권3호
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• pp.142-153
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• 2021

Liver fibrosis is a wound-healing response to chronic liver injury, which is caused by the continuous and excess deposition of extracellular matrix (ECM). The aim of this study is to investigate whether Uncaria rhynchophylla water extract (UR) can ameliorate thioacetamide (TAA)-induced liver fibrosis. The liver fibrosis model was induced on C57BL/6 mice by intraperitoneal injection with TAA three times a week for 8 weeks. UR (200 mg/kg) or silymarin (50 mg/kg) was administered orally daily for 8 weeks. Biochemical analyses including AST, ALT, MPO, and Ammonia levels were measured in serum. In the mice liver tissues, western blot and histological staining were analyzed. As a result, UR dramatically reduced the levels in serum AST, ALT, MPO, and Ammonia levels. UR treatment regulated NADPH oxidase factors expression, and antioxidant enzymes except for GPx-1/2 were significantly increased via Nrf2 activation. Furthermore, pro-inflammatory mediators, such as COX-2 and iNOS were markedly suppressed through the inhibition of NF-κB activation. Expressions of ECM-related protein including α-SMA and Collagen I were noticeably decreased. The additional histological evaluation confirmed that hepatocyte damage and collagenous fiber accumulation were attenuated. Taken together, these data suggest that UR possessed hepatoprotective effects in TAA-induced liver fibrosis via the NF-κB inactivation and Nrf2 activation. Therefore, UR may act as a potential therapeutic drug against liver fibrosis.

• 약학회지
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• 제44권1호
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• pp.22-28
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• 2000

The extracts of Euphoria longan, Ziryphus jujuba, Thuja orientalis, Polygala tenuifolia, Acorus gramineus, Cyperus rotundus, Poria cocos, Uncaria rhynchophylla, and Albizzia julibrissin, which have been used as sedative drugs in Korean folk medicine, were evaluated for their effects on neurotransmission and antioxidative system in vitro. Among the tested drugs, Acorus gramineus showed most inhibitory activities on monoamine oxidase, xanthine oxidase, aldehyde oxidase, and lipid peroxidation and Uncaria rhynchophylla also inhibited most effectively GABA transaminase and DPPH radical.

• Journal of the Korean Wood Science and Technology
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• 제38권4호
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• pp.292-297
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• 2010

일본산 산공재 중 구조적 특징이 목재흡음에 적합하다고 사료되는 카기카즈라목재의 흡음성능과 구조적 특징을 관찰하고자 목재횡단방향으로 원반형시험편을 채취하여 구조적 특징을 관찰하고, 전달함수법으로 흡음율을 측정하였다. 측정주파수범위에서 카기카즈라 횡단면의 흡음율은 0.30에서 0.50의 수치를 나타내었다. 비교수종인 낙엽송시험편보다 2~3배 높은 흡음율을 나타내었으며, 특히 2~4 KHz의 주파수영역에서는 50% 전후로 높은 흡음율을 나타내었다. 카기카즈라목재의 횡단면에서는 다수의 커다란 도관이 존재하고 이들 도관요소들은 단일천공판으로 연결되어 다공질형흡음에 유용한 연속된 모세관이 다량 존재하는 사실을 확인할 수 있었다.

• 생약학회지
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• 제28권4호
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• pp.167-173
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• 1997

We have tested 101 species traditional medicinal plants on Acetylcholinester ase inhibition. Among them. Caesalpina sappan. Cnidium offcinale, Coptis japonica, Ephedra sinica, Eribotrya japonica, Gardenia jasminoides, Liriope platyphylla, Lonicera japonica, Paeonia suffruticosa, Phyllostachys nigra, Polygonum multiflorum, Prunella vulgaris, Rheum undulatum, Salvia miltiorrhiza and Uncaria rhynchophylla showed strong inhibition potency over 88%. In addition, Aconitum carmichaeli, Caesalphina sappan, Cinnamomun japonica, Citrus unshiu, Hordeum vulgare, Glycine max and Rehmannia glutinosa showed relatively good inhibition potency over 80%.

• Journal of Microbiology and Biotechnology
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• 제12권4호
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• pp.664-668
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• 2002

Antioxidants either scavenge superoxide and free radicals or stimulate the detoxification mechanisms within cells, resulting in increased detoxification of free radicals formation. Hyperin, isolated from the stem of Uncaria rhynchophylla, prevented oxygen radical formation and inhibited lipid oxidation. The effective concentrations were 31.3 $\mu$M for a radical scavenging assay and 2.2 $\mu$M for a microsome assay. cDNA microarray analysis to determine which genes were modulated by hyperin found that 50 genes were upregulated and 37 genes were downregulated in SNU-668 human gastric cancer cells. Among these genes, thirteen genes that were significantly affected by hyperin were verified by RT-PCR for their effect of genetic reprogramming.