• Title/Summary/Keyword: Ulp2

Search Result 12, Processing Time 0.024 seconds

Yeast Small Ubiquitin-Like Modifier (SUMO) Protease Ulp2 is Involved in RNA Splicing

  • Jeong-Min Park;Seungji Choi;Dong Kyu Choi;Hyun-Shik Lee;Dong-Hyung Cho;Jungmin Choi;Hong-Yeoul Ryu
    • Development and Reproduction
    • /
    • v.28 no.2
    • /
    • pp.47-54
    • /
    • 2024
  • In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in Saccharomyces cerevisiae, the SUMO protease, Ulp2, is involved in splicing. In the ulp2Δ mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.

Removal of Uranium from U-bearing Lime-Precipitate using dissolution and precipitation methods (우라늄 함유 석회침전물의 용해 및 침전에 의한 U 제거)

  • Lee, Eil-Hee;Lee, Keun-Young;Chung, Dong-Yong;Kim, Kwang-Wook;Lee, Kune-Woo;Moon, Jei-Kwon
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
    • /
    • v.10 no.2
    • /
    • pp.77-85
    • /
    • 2012
  • This study was carried out to remove (/recover) the uranium from the Uranium-bearing Lime Precipitate (ULP). An oxidative dissolution of ULP with carbonate-acidified precipitation and a dissolution of ULP with nitric acid-hydrogen peroxide precipitation were discussed, respectively. In point of view the dissolution of uranium in ULP, nitric acid dissolution which could dissolved more than 98% of uranium was more effective than carbonate dissolution. However, in this case, uranium was dissolved together with a large amount of impurities such as Al, Ca, Fe, Mg, Si, etc. and some impurities were also co-precipitated with uranium during a hydrogen peroxide precipitation. On the other hand, in the case of carbonate dissolution-acidified precipitation, U was dissolved less than 90%. Therefore, it was less effective than nitric acid dissolution for the volume reduction of radioactive solid waste. However, it was very effective to recover the pure uranium, because impurities were hardly dissolved and hardly co-precipitated with uranium.

A Sub-${\mu}$W 22-kHz CMOS Oscillator for Ultra Low Power Radio (극저전력 무선통신을 위한 Sub-${\mu}$W 22-kHz CMOS 발진기)

  • Na, Young-Ho;Kim, Jong-Sik;Kim, Hyun;Shin, Hyun-Chol
    • Journal of the Institute of Electronics Engineers of Korea SD
    • /
    • v.47 no.12
    • /
    • pp.68-74
    • /
    • 2010
  • A sub-${\mu}$W CMOS Wien-Bridge oscillator for ultra low power (ULP) radio applications is presented. The Wien-Bridge oscillator is based on an non-inverting opamp amplifier with a closed-loop gain $1+R_2/R_1$ as a means of providing necessary loop gain. An additional RC network provides appropriate phase shift for satisfying the Barkhausen oscillation condition at the given frequency of 1/($2{\pi}RC$). In this design, we propose a novel loop gain control method based on a variable capacitor network instead of a rather conventional variable resistor network. Implemented in $0.18{\mu}m$ CMOS, the oscillator consumes only 560 nA at the oscillation frequency of 22 kHz.

Packet Loss Protection Method of Scalable Video considering Perceptual Saliency (시각 특성을 고려한 스케일러블 비디오의 패킷 손실 최적화 기법)

  • Lee, Hyunho;Lee, Kwanghyun;Lee, Sanghoon
    • Proceedings of the Korean Society of Broadcast Engineers Conference
    • /
    • 2011.07a
    • /
    • pp.563-564
    • /
    • 2011
  • 본 논문에서 우리는 unequal loss protection(ULP) 알고리즘을 기반으로 패킷이 손실될 수 있는 망 환경에서 인지적으로 재구성된 영상의 왜곡을 최소화하는 방법을 제안한다. 알고리즘에는 2가지의 주요 요인이 있다. 첫째, 인간 시각 체계의 균일하지 않은 분포의 함수로 압축된 영상에 가중치를 준다. 둘째, 패킷은 오류 전파가 일어나지 않도록 하면서 각각의 group of picture(GOP)내에서 시간적인 중요성이 할당된다. 프레임의 인지적인 중요성과 GOP의 계층적인 중요성을 동시에 고려하여, 제안하는 ULP알고리즘은 인간 시각적으로 가장 중요한 지역의 크기를 식별하여 효율적인 forword error correction(FEC) 알고리즘을 수행한다.

  • PDF

Hardware Design of Pipelined Special Function Arithmetic Unit for Mobile Graphics Application (모바일 그래픽 응용을 위한 파이프라인 구조 특수 목적 연산회로의 하드웨어 설계)

  • Choi, Byeong-Yoon
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.17 no.8
    • /
    • pp.1891-1898
    • /
    • 2013
  • To efficiently execute 3D graphic APIs, such as OpenGL and Direct3D, special purpose arithmetic unit(SFU) which supports floating-point sine, cosine, reciprocal, inverse square root, base-two exponential, and logarithmic operations is designed. The SFU uses second order minimax approximation method and lookup table method to satisfy both error less than 2 ulp(unit in the last place) and high speed operation. The designed circuit has about 2.3-ns delay time under 65nm CMOS standard cell library and consists of about 23,300 gates. Due to its maximum performance of 400 MFLOPS and high accuracy, it can be efficiently applicable to mobile 3D graphics application.

Novel Pass-transistor Logic based Ultralow Power Variation Resilient CMOS Full Adder

  • Guduri, Manisha;Islam, Aminul
    • JSTS:Journal of Semiconductor Technology and Science
    • /
    • v.17 no.2
    • /
    • pp.302-317
    • /
    • 2017
  • This paper proposes a new full adder design based on pass-transistor logic that offers ultra-low power dissipation and superior variability together with low transistor count. The pass-transistor logic allows device count reduction through direct logic realization, and thus leads to reduction in the node capacitances as well as short-circuit currents due to the absence of supply rails. Optimum transistor sizing alleviates the adverse effects of process variations on performance metrics. The design is subjected to a comparative analysis against existing designs based on Monte Carlo simulations in a SPICE environment, using the 22-nm CMOS Predictive Technology Model (PTM). The proposed ULP adder offers 38% improvement in power in comparison to the best performing conventional designs. The trade-off in delay to achieve this power saving is estimated through the power-delay product (PDP), which is found to be competitive to conventional values. It also offers upto 79% improvement in variability in comparison to conventional designs, and provides suitable scalability in supply voltage to meet future demands of energy-efficiency in portable applications.

Preventing Procurement Fraud in E-purchasing for Indonesian Local Governments

  • ZAHRA, Femilia;ABDULLAH, Muhammad Ikbal;KAHAR, Abdul;DIN, Muhammad;NURFALAH, Nurfalah
    • The Journal of Asian Finance, Economics and Business
    • /
    • v.8 no.2
    • /
    • pp.505-511
    • /
    • 2021
  • This study aims to investigate the effects of e-purchasing on increasing transparency, expanding market access, and the efficiency of the government procurement process. This study also investigates how transparency of the procurement process, improved market access and the efficiency of the procurement process in e-purchasing will decrease the level of fraudulent government procurement in Indonesia. The questionnaires were distributed to the Procurement Service Units (ULP) of Local Governments through email. There are currently 542 ULPs in Indonesia based on the data of the Government Procurement Policy Institute (LKPP). However, only 520 ULPs had their email addresses traced, 120 ULP returned the questionnaires and the response rate is lower than it was expected. The data research was analyzed by a structural equation model (SEM) by using WarpPLS 7.0. The results reveal that the e-purchasing effect on the transparency, the expansion of market access, and the efficiency of the government procurement process. The other findings show the negative effect between efficiency in government procurement and fraud in government procurement. The findings of this study suggest that efficiency in the process of government procurement will minimize fraud in government procurement. However, transparency in government procurement and the expansion of market access was not sufficient to minimize fraud in government procurement.

Construction of a Bile-responsive Expression System in Lactobacillus plantarum

  • Chae, Jong Pyo;Pajarillo, Edward Alain;Hwang, In-Chan;Kang, Dae-Kyung
    • Food Science of Animal Resources
    • /
    • v.39 no.1
    • /
    • pp.13-22
    • /
    • 2019
  • This study aimed to develop a bile-responsive expression system for lactobacilli. The promoters of four genes, encoding phosphoenolpyruvate-dependent sugar phosphotransferase (mannose-specific), L-lactate dehydrogenase (LDH), HPr kinase, and D-alanine-D-alanine ligase, respectively, which were highly expressed by bile addition in Lactobacillus johnsonii PF01, were chosen. Each promoter was amplified by polymerase chain reaction and fused upstream of the ${\beta}$-glucuronidase gene as a reporter, respectively. Then, these constructs were cloned into E. coli-Lactobacillus shuttle vector pULP2, which was generated by the fusion of pUC19 with the L. plantarum plasmid pLP27. Finally, the constructed vectors were introduced into L. plantarum for a promoter activity assay. The LDH promoter showed the highest activity and its activity increased 1.8-fold by bile addition. The constructed vector maintained in L. plantarum until 80 generations without selection pressure. A bile-responsive expression vector, $pULP3-P_{LDH}$, for Lactobacillus spp. can be an effective tool for the bile-inducible expression of bioactive proteins in intestine after intake in the form of fermented dairy foods.

Analysis of the Hardware Structures of the IoT Device Platforms for the Minimal Power Consumption (소비 전력 최소화를 위한 IoT 디바이스 플랫폼의 하드웨어 구조 분석)

  • Lee, Jin
    • Journal of Internet of Things and Convergence
    • /
    • v.6 no.2
    • /
    • pp.11-18
    • /
    • 2020
  • Since the end devices of the Internet of Things (IoT) are battery operated products, careful consideration for ultra-low power (ULP) is required. The Micro Controller Unit (MCU) industry has developed very effective functions to save energy, but developers have difficulty in selecting the MCU because various operating modes are applied to reduce energy consumption by manufacturers. Therefore, this paper introduces ULPMark benchmark, a standardized benchmark method that can compare MCUs of various vendors and feature sets, and provides hardware functions for ultra-low-power operation of the two platforms that received the high evaluation scores from ULPMark. In addition, we investigated and analyzed how developers can utilize the functions for ultra low power consumption through driver APIs and detailed register control.