• Quantitative Bio-Science
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• v.37 no.2
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• pp.103-111
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• 2018

This study was performed to investigate the cytoprotective effects of Stachys riederi var. japonica ethanol extract (SREE) to control oxidative stress induced by UVA-irradiation by examining antioxidant capacity and gene expression of cytochrome c using human dermal fibroblasts. The total polyphenolics and flavonoids in the SREE were 41.2 and 25.4 mg/g, respectively. At concentrations of 500 and $1000{\mu}g/mL$, the electron-donating ability of SREE was 48.6% and 82.0%, respectively, and the 2,2'-azino-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity was 62.3% and 78.8%, respectively. These findings showed that SREE has a fairly good antioxidant capacity. As determined by an MTT assay, the maximum permissible level for treating SREE to human dermal fibroblasts was shown to be over $200{\mu}g/mL$. SREE ($200{\mu}g/mL$) significantly decreased cytochrome c mRNA and protein expression by 31.1% (p<0.001) and 38.8% (p<0.01), respectively. These findings suggest that SREE may protect human skin cells against mitochondrial-dependent apoptosis. Therefore, SREE seems to be a natural antioxidant to protect cells against oxidative stress induced by UVA-irradiation.

• Journal of Photoscience
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• v.9 no.3
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• pp.49-50
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• 2002

N-Nitrosoproline(NPRO) is endogenously formed from proline and nitrite. NPRO has been reported to be nonmutagenic and noncarcinogenic. In this study, we have detected the direct mutagenicity of NPRO with UVA and UVB towards S. typhimurium. Formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a mutagenic lesion, was observed in calf thymus DNA treated with NPRO plus simulated sunlight. Furthermore, the treatment with NPRO and sunlight induced single strand breaks in the superhelical replicative form of phage M13mp2 DNA. An analysis using scavengers suggested that both reactive oxygen species and NO radical mediate the strand breaks. The formation of nitric oxide was observed in NPRO solution irradiated with UVA. The co-mutagenic and co-toxic actions of NPRO and sunlight merit attention as possible mechanisms increasing the carcinogenic risk from UVA irradiation.

• Toxicological Research
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• v.29 no.1
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• pp.35-42
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• 2013

Quantum dots (QDs) have received considerable attention due to their potential role in photosensitization during photodynamic therapy. Although QDS are attractive nanomaterials due to their novel and unique physicochemical properties, concerns about their toxicity remain. We suggest a combination strategy, CdSe/ZnS QDs together with curcumin, a natural yellow pigment from turmeric, to reduce QD-induced cytotoxicity. The aim of this study was to explore a potentially effective cancer treatment: co-exposure of HL-60 cells and human normal lymphocytes to CdSe/ZnS QDs and curcumin. Cell viability, apoptosis, reactive oxygen species (ROS) generation, and DNA damage induced by QDs and/or curcumin with or without ultraviolet A (UVA) irradiation were evaluated in both HL-60 cells and normal lymphocytes. In HL-60 cells, cell death, apoptosis, ROS generation, and single/double DNA strand breaks induced by QDs were enhanced by treatment with curcumin and UVA irradiation. The protective effects of curcumin on cell viability, apoptosis, and ROS generation were observed in normal lymphocytes, but not leukemia cells. These results demonstrated that treatment with QD combined with curcumin increased cell death in HL-60 cells, which was mediated by ROS generation. However, curcumin acted as an antioxidant in cultured human normal lymphocytes.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.5 s.142
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• pp.617-625
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• 2005

The purpose of this study was to develop functional and environment-friendly polyester fabric by irradiating Ultra-Violet, which was produced by the low-pressure mercury lamp. UV irradiation was conducted with various treatment times and distances. Also, pretreatments of solvents and photoinitiator were used to improve the surface modification effects. The effects of UV irradiation on the moisture regain were found to increase gradually with increase of UV treatment time and decrease of treatment distance. Compared the effects of UVC and UVA, UVC was more effective than UVA. Moisture regain and wicking of PET was increased after UV treatment in our experimental condition. And owing to increasing of moisture regain, static charge was decreased. Pretreatment of solvents such as methanol, ethyl ether and addition of photoinitiator such as Benzophenone accelerated surface modification. The moisture regain was increased but wicking was decreased with pretreatment and addition of photoinitiatior. Therefore it is considered as inappropriate f3r clothing because of wicking effects. Yellowness, tensile strength and elasticity slightly decreased after UV irradiation.

• Korean Journal of Veterinary Pathology
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• v.1 no.1
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• pp.40-45
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• 1997

The phototoxic potentials of LB20304a a new quinolone compound being developed by LG Chemical Ltd, and reference compounds (Ciprofloxacin; CPFX, Enoxacin; ENX and Lomefloxacin; LFLX) were compared in a murine model. in addition photostability of these compunds was studied after irradiation with long-wave UV light(UVA, 0, 0.3 1 or 3 Joule/$Cm^2$) When hairless mice(9 to 11 weeks old 19-26g) were orally administered with different dose levels of test compunds and exposed to UVA(40J/$cm^2$) inflammatory reactions were observed in a dose dependent manner. Among the compounds tested, LB20304a demonstrated the least phototoxic effects and showed no inflammatroy lesions at a dose level of 100mg.kg (Low dose). ENX and LFLX demonstrated much greater phototoxic reactions while CPFX showed similar or slightly greater phototoxic reactions compared to LB20304a. Similar to the in-vivo results the solutions of LB20304a and CPFX irradiated with UVA demonstrated reduced spectral changes compared to those of ENX and LFLX. In conclusion these data suggest that phototoxic potencies of the quinolones tested were; LFLX > ENX > CPFX $\geq$ LB20304a. No phototoxic dose of LB20304a in mice was 100 mg/kg.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.18 no.1
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• pp.64-80
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• 1992

Considerable interest has been generated in age-related non-enzymatic glycosylation and crosslinking of collagen in view of its extracellular nature, and its long biological half-life. The effects of UVA, which penetrates deep in dermis, and reactive oxygen species (ROS) on age- related changes of dermal collagen were studied. The amount of nonenzymatic glycosylation, fragmentation, and crosslinking of collagen were monitored from the mixtures of Type I collagen from calf skin and glucose, irradiated by UVA, with or without scavengers of ROS. At both high and low glucose dosages, non-enzymatic glycosylation was not affected by UVA irradiation. At high glucose dosage, however, glycosylation was reduced by the scavengers of superoxide radical and singlet oxygen, bolt not by hydroxyl radical scavengers. Fragmentation was increased by UVA and decreased by all ROS scavengers. Crosslinking was also enhanced by UVA, and effectively blocked crosslinking. Superoxide radical and singlet oxygen, which were produced by autoxidation of glucose independently to UVA, may encounter the initial phase of glycation. ROS generated from Amadory compounds by UVA enhanced fragmentation and crosslinking Hydroxyl radical was thought to be a major ROS affecting crosslinking. These results suggest that UVA and ROS are able to enhance age-related structural changes of collagen, as affecting many other tissue and cellular components.

• Journal of Photoscience
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• v.9 no.2
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• pp.485-487
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• 2002

Cells receive signals for survival as well as death, and the balance between the two ultimately determines the fate of the cells. UV-triggered apoptotic signaling has been well documented, whereas UV-induced survival effects have received little attention. We have reported previously that UVB irradiation prevented apoptosis, which was partly dependent on activation of the phosphatidylinositol 3-kinase (PI3-kinase)/ Akt pathway. In this study, anti-apoptotic effects of UV with different wavelength ranges, UVA, UVB and UVC, were examined. NIH3T3 cells showed apoptotic cell death by detachment from the extracellular matrix under serum-free conditions, which was prevented by all wavelengths. However, the effect of UVA was less than those of UVB and UVC. Reduction of mitochondrial transmembrane potential and activation of caspase-9 and -3 were suppressed by all three wavelengths of UV, showing wavelength-dependent effects as mentioned above. The PI3-kinase inhibitor wortmannin partially inhibittrl the UVB and UVC-induced suppression of apoptosis, but not the inhibitoty effect of UVA. The Akt phosphotylation by UVB and UVC was completely inhibittrl by addition of wortmannin, but that by UVA was not P38 MAP kinase inhibitor SB203580 partially inhibited the UVB and UVC-induced suppression of apoptosis and Akt phosphotylation, and completely inhibited UVA-induced those. These results suggested the existence of two different survival pathways leading to suppression of apoptosis, one for UVA that is independent of the PI3-kinase/Akt pathway and dependent on p38 MAP kinase, and the other for UVB and UVC that is dependent on both pathways.

• Journal of Microbiology and Biotechnology
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• v.23 no.10
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• pp.1357-1364
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• 2013

Human skin is continuously exposed to ultraviolet (UV)-induced photoaging. UVA increases the activity of MMP-1 in dermal fibroblasts through mitogen-activated protein kinase (MAPK), p38, signaling. The irradiation of keratinocytes by UVA results in the secretion of the inflammatory cytokine, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and the stimulation of MMP-1 in normal human dermal fibroblasts (NHDFs). Lipoteichoic acid (LTA) is a component of the cell wall of gram-positive Lactobacillus spp. of bacteria. LTA is well known as an anti-inflammation molecule. LTA of the bacterium Lactobacillus plantarum has an anti-photoaging effect, but the potential anti-photoaging effect of the other bacteria has not been examined to date. The current study showed that L. sakei LTA (sLTA) has an immune modulating effect in human monocyte cells. Our object was whether inhibitory effects of sLTA on MMP-1 are caused from reducing the MAPK signal in NHDFs. It inhibits MMP-1 and MAPK signaling induced by UVA in NHDFs. We also confirmed effects of sLTA suppressing TNF-${\alpha}$ inducing MMP-1 in NHDFs.

• Journal of Preventive Medicine and Public Health
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• v.30 no.1 s.56
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• pp.145-155
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• 1997

Interview survey and dermatological examination have been performed to investigate the health problems of workers continuously exposed to coal-tar pitch. The phototoxicity of coal-tar pitch was confirmed by the photopatch tests for six healthy adults. The main results are followings; 1. There was no special history of allergic diseases in both the exposed and non-exposed group. 2. The frequency of the phototoxic dermatosis and the coal-tar acne in the exposed group was significantly greate.(p<0.05) than that of the control group. In the exposed group, the phototoxic dermatosis and the coal-tar acne were observed in nine workers(90%) and seven workers(70%), respectively. However, those, disease were not observed in the control group. 3. Five results(83%) were positive to the photopatch test for coal-ta. pitch 48 hours after UVA irradiation. But the lesion was subsiding 72 hours after UVA irradiation. 4. Malignant cancers were not reported among workers ever exposed to coal-tar pitch. In conclusion, it appears that workers exposed to coal-tar pitch have high risks of phototoxic dermatosis and coal-tar acne. A health policy should be provided to prevent phototoxic dermatosis among coal-tar pitch workers. More studies are required to determine malignancy.

• Microbiology and Biotechnology Letters
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• v.52 no.1
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• pp.65-75
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• 2024

The growing demand for functional cosmetics, driven by modern individuals' quest for healthy and aesthetically pleasing skin, is being challenged by reports of side effects and toxicity associated with these products. This underscores the importance of exploring natural plant-based materials for functional cosmetics. This research focuses on the assessment of antioxidant and whitening properties of mixed extracts from Ipomoea nil, Arctostaphylos uva-ursi, and Angelica gigas Nakai, all of which have proven pharmacological benefits. The study evaluated the extracts' total polyphenol and flavonoid contents, ABTS and DPPH radical scavenging activities, SOD-like activity, and xanthine oxidase inhibition to determine their antioxidant capabilities. Moreover, the whitening potential was investigated through tyrosinase activity and melanin production assays, alongside a cytotoxicity evaluation via a cell viability test. The findings revealed that the extracts, IAA-1 to IAA-4, demonstrated both antioxidative and whitening capabilities without exhibiting cytotoxic effects. Notably, the extract IAA-4, processed through ultrasonic and ultrahigh pressure extraction, exhibited superior effectiveness. These results indicate that the cavitation formed during ultrasonic irradiation effectively destroys the plant cell wall by creating high pressure, and as a result, it is judged that useful components are easily extracted. As a result of the study, it was confirmed that the IAA-4 extract could be applied as a material for functional cosmetics.