• Title/Summary/Keyword: UV살균

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The Effect of Ultraviolet-C Radiation on Disinfection (Ultraviolet-C 조사의 살균 효과)

  • Choi, Houng-Sik;Choi, Kyu-Hwan;Park, So-Yeon
    • Physical Therapy Korea
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    • v.9 no.3
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    • pp.93-99
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    • 2002
  • Traditionally, ultraviolet (UV) has been used for treating the pressure sore and skin wound. The effects of UVA and UVB radiation on disinfection have been reported. The purpose of this study was to examine the effectiveness of UVC radiation on disinfection of Escherichia coli, Staphylococcus aureus, Salmonella typhimurium in vitro. Three bacterium were radiated by UVC (250 nm, 20 seconds) and incubated at $37^{\circ}C$ for 24 hours at the agar culture medium. Kill rates of all three bacterium were 99.9%. UVC radiated on three kinds of bacterium for 30 or 60 seconds. Kill rates were 99.9% both 30 and 60 seconds. This data suggests that UV light at 250 nm could be a useful method to minimize infection and shorten healing time in pressure sore and skin wound condition.

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Disinfection Characteristic of Sewage Wastewater Treatment Using Solar Light/TiO2 Film System (태양광/광촉매를 이용한 오폐수 살균특성)

  • Cho Il-Hyoung;Lee Nae-Hyun;An Sang-Woo;Kim Young-Kyu;Lee Seung-Mok
    • Journal of Environmental Science International
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    • v.15 no.7
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    • pp.677-688
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    • 2006
  • Currently, the application of $TiO_2$ photocatalyst has been focused on purification and treatment of wastewater. However, the use of conventional $TiO_2$ slurry photocatalyst results in disadvantage of stirring during the reaction and of separation after the reaction. And the usage of artificial UV lamp has made the cost of photocatalyst treatment system high. Consequently, we studied that solar light/$TiO_2$ film system was designed and developed in order to examine disinfection characteristics of sewage wastewater treatment. The optimum conditions for disinfection such as solar light intensity, characteristic of sewage wastewater, amounts of $TiO_2$ and comparison of solar ligth/$TiO_2$ systems with UV light/$TiO_2$ system was examined. The results are as follows: (1) photocatalytic disinfection process with solar light in the presence of $TiO_2$ film more effectively killed total coliform (TC) than solar light or $TiO_2$ film absorption only. (2) The survival ratio of TC and residual ratio of organic material (BOD, CODcr) decreased with remain resistant material. (3) The survival ratio of TC and residual ratio of organic material (BOD, CODcr) decreased with the increase of amounts of $TiO_2$. (4) TC survival ratio decreased linearly with increasing UV light intensity. (5) The disinfection effect of solar light/$TiO_2$ slurry system decreased more than UV light/$TiO_2$ film systems. (6) The disinfection reaction followed first-order kinetics. We suggest that solar light instead of using artificial UV light was conducted to investigate the applicability of alternative energy source in the disinfection of TC and the degradation of organic material.

Combined Effect of Cold Plasma and UV-C Against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on Fresh-cut Lettuce (양상추에 인위접종된 Escherichia coli O157:H7, Salmonella Typhimurium과 Listeria monocytogenes에 대한 저온 플라즈마와 UV-C의 살균 효과)

  • Seong, Ji-Yeong;Park, Mi-Jung;Kwon, Ki-Hyun;Oh, Se-Wook
    • Journal of Food Hygiene and Safety
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    • v.32 no.1
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    • pp.64-69
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    • 2017
  • This study was conducted to investigate the effect of cold plasma combined with UV-C irradiation against Escherichia coli O157:H7, Salmonella enterica serovar Typhimurium, and Listeria monocytogenes on lettuce. E. coli O157:H7, S. Typhimurium, and L. monocytogenes, corresponding to approximately 5.82, 5.09, 5.65 log CFU/g, were inoculated on lettuce, respectively. Then, the lettuce was treated with cold plasma, UV-C and combination (cold plasma + UV-C), respectively. The treated lettuce was stored for 9 days at $4^{\circ}C$ for microbiological analysis and sensory evaluation. Cold plasma reduced the populations of E. coli O157:H7, S. Typhimurium, and L. monocytogenes by 0.26, 0.65, and 0.93 log CFU/g, respectively. Each microorganism were reduced by 0.87, 0.88, and 1.14 log CFU/g after UV-C treatment. And, the combined treatment that was treated by cold plasma after UV-C treatment reduced the populations of inoculated microorganisms by 1.44, 2.70, 1.62 log CFU/g, respectively. The all treatment significantly (p < 0.05) reduced the populations of all inoculated bacteria compared to untreated lettuce. UV-C combined with cold plasma was the most effective for reducing the pathogenic bacteria on lettuce, by showing log-reductions of ${\geq}2.0\;log\;CFU/g$. All treatment was not significantly different until 6 day storage compared to control group in terms of appearance, texture and overall acceptability. Therefore, the combined treatment will be an effective intervention method to control the bacteria on lettuce.

Inhibition Effect of Bacillus subtilis on 365 nm UV-LED Irradiation According to Packaging Materials (포장재 조건에 따른 365 nm UV-LED 조사의 Bacillus subtilis 생육 억제 효과)

  • Lee, Da-Hye;Jeong, So-Mi;Xu, Xiaotong;Kim, Koth-Bong-Woo-Ri;Ahn, Dong-Hyun
    • Microbiology and Biotechnology Letters
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    • v.47 no.3
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    • pp.332-336
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    • 2019
  • The use of ultraviolet (UV) spectroscopy for foods is known to have a microbial inhibitory effect. UV-A having a longer wavelength than UV-C can be used for continuous or intermittent UV irradiation of food stored in containers or packages. Because UV-LED can be used effectively at a low price, this study reported the effect of UV-A 365 nm-LED on inhibiting Bacillus subtilis in accordance with the packaging conditions employed in daily use. The packaging materials were linear low-density polyethylene (LLD-PE), nylon/low density polyethylene (LDPE), polystyrene, and glass. When all packaging materials were treated with 365 nm UV-LED, B. subtilis was observed to remain inactive for 30-60 min. Further, compared with the control (-log 5), the survival rate of B. subtilis was -log 2.0-2.5 for nylon/LDPE and -log 2.58-3.61 for LLD-PE. These packaging materials showed an excellent inhibitory effect regardless of their thickness. Typically, a decrease in the viable cell count of more than 3 log indicates a 99.9% bactericidal effect. These results suggest that 365 nm UV-LED permeated the packaging material and inhibited bacterial growth.

Microbial Decontamination of Black Pepper Powder Using a Commercial-scale Intervention System Combining Ultraviolet-C and Plasma Treatments (Ultraviolet-C와 플라즈마를 병합 처리하는 양산형 살균 시스템을 이용한 후춧가루 미생물 저해)

  • Bang, In Hee;Lee, Seung Young;Han, Kyoon Sik;Min, Sea C.
    • Food Engineering Progress
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    • v.22 no.4
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    • pp.386-391
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    • 2018
  • Effects of a commercial scale intervention system combining ultraviolet (UV)-C and plasma treatments on the microbial decontamination of black pepper powder were investigated. The process parameters include treatment time, time for plasma accumulation before treatment, and water activity of black pepper powder. A significant reduction in the number of indigenous aerobic mesophilic bacteria in black pepper powder was observed after treatments lasted for ${\geq}20min$ (p<0.05) and the reduction was differed by powder manufacturer. The microbial reduction rates obtained by individual UV-C treatment, individual plasma treatment, and UV-C/plasma-combined treatment were 0.2, 0.5, and 1.0 log CFU/g, respectively, suggesting that the efficacy of the microbial inactivation was enhanced by treatment combination. Nonetheless, neither plasma accumulation time nor powder water activity affected the microbial inactivation efficacy of the combined treatment. The UV-C/plasma-combined treatment, however, decreased lightness of black pepper powder, and the decrease generally increased as operation time increased. The plasma accumulation time of 20 min resulted in significant reduction in both lightness and brown color. The results indicate that the commercial-scale intervention system combining treatments of UV-C and plasma has the potential to be applied in the food industry for decontaminating black pepper powder.

Design and Fabrication of a Ballast Water Treatment System Using UV Lamps (자외선램프를 이용한 선박평형수 처리시스템의 설계 및 제작)

  • Pyo, Tae-Sung;Cheon, Sang-Gyu;Park, Dae-Won;Choi, Sung-Kuk;Kim, Seong-Yeon;Kil, Gyung-Suk
    • Journal of Advanced Marine Engineering and Technology
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    • v.33 no.6
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    • pp.952-958
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    • 2009
  • The International Maritime Organization (IMO) has adopted the ballast water management convention at a diplomatic conference in early 2004 that all ships should be equipped with a treatment system from 2010 gradually. In this paper, the disinfection characteristic of ultra-violet (UV) rays was studied and a ballast water treatment system (BWTS) which can treat $50m^3$/h sea water was manufactured. The system consists of a disinfection chamber with six 3.5 kW UV lamps which are operated by magnetic ballasts, a programmable logic controller (PLC) and set of pipe lines. The biological disinfection efficacy of the prototype BWTS was evaluated following the IMO rules using zooplankton such as Artemia and Rotifer species for the size over $50{\mu}m$, and phytoplankton such as Tetraselmis and Thalassiosira species for the size between 10 to $50{\mu}m$. From the experimental results, the disinfection efficacy was 99.99 % that meets the IMO requirement. However, more studies on an energy saving system are needed because the consumption power of the prototype system is as high as over 21 kWh for $50m^3$/h.

Effect of Physical Control Technology on Aspergillus ochraceus Reduction (물리적 제어기술이 Aspergillus ochraceus 저감화에 미치는 영향)

  • Lee, Eun-Seon;Kim, Jong-Hui;Kim, Bu-Min;Oh, Mi-Hwa
    • Journal of Food Hygiene and Safety
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    • v.36 no.5
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    • pp.447-453
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    • 2021
  • In this study, the effectiveness of physical control technology, a combined light sterilization (LED, UV) and hot water treatment in reducing Aspergillus ochraceus for food production environment was investigated. In brief, 1 mL aliquot of A. ochraceus spore suspension (107-8 spore/mL) was inoculated onto stainless steel chips, which was then dried at 37℃, and each was subjected to different physical treatment. Treatments were performed for 0.5, 1, 2, 5, 8, and 11 hours to reduce the strains using a light-emitting diode, but no significant difference was confirmed among the treatments. However, a significant reduction was observed on the chips treated with UV-C exposure and hot water immersion. After being treated solely with 360 kJ/m2 of UV-C on stainless steel chip, the fungi were significantly reduced to 1.27 log CFU/cm2. Concerning the hot water treatment, the initial inoculum amount of 6.49 log CFU/cm2 was entirely killed by immersion in 83℃ water for 5 minutes. Maintaining a high temperature for 5 minutes at the site is difficult. Thus, considering economic feasibility and usability, we attempted to confirm the appropriate A. ochraceus reduction conditions by combining a relatively low temperature of 60℃ and UV rays. With the combined treatments, even in lukewarm water, A. ochraceus decreased significantly through the increases in the immersion time and the amount of UV-C irradiation, and the yield was below the detection limit. Based on these results, if work tools are immersed in 60℃ lukewarm water for 3 minutes and then placed in a UV sterilization device for more than 10 minutes, the possibility of A. ochraceus cross-contamination during work is expected to be reduced.

Development of Real-time Groundwater Quality Monitoring and Advanced Groundwater Purification Technology for Groundwater using Photoinduced Reactive Oxygen Species (지하수 수질 실시간 모니터링 및 광유도 활성산소를 이용한 고도수처리 기술)

  • Kang-Kyun Wang;Byung-Woo Kim
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.15-15
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    • 2023
  • 2020년 기준 국내 상수도 보급률은 99.1% 차지하고 있으며(환경부, 2019), 수도관리차원에서 수돗물은 먹는 물로 시판되어질 만큼 우수한 관리체계를 유지하고 있다. 그 반면에 지하수는 생활용수, 식품가공, 농·축산, 양어, 군부대를 비롯한 전국지역에서 연간 10억 8천만톤 용수를 소비하고 있음에도 (환겨례 신문, 2013; 환경부, 2019) 사용되는 지하수의 약 65%가 음용수 불가판정을 받았으며, 최근 지하수의 오염비율은 급격히 증가하는 추세이다. 특히, 지하수관정의 관리부주의에 의한 수질오염 및 수인성 다제내성균(슈퍼박테리아) 등에 의한 오염사례가 국내는 물론, 국제적으로 다수 보고되고 있는 실정이다 (환경부, 2013). 현재 지하수 수질관리는 공공기관 및 지자체 지정기관을 통해 진행되고 있으며, 검사기간은 수질채취로부터 통상 7~15일정도 소요되어 수질 관리 및 기준, 검사주기에 대한 애로가 많다. 현장 지하수관정에서 실시간 수질을 모니터링하고 이에 연동된 자동 수처리 시스템의 개발 및 도입은 나날이 심각해지는 환경오염 상황에서 선제적 예방과 해결방법으로 중요한 요소기술이다. 현재 지하수오염 및 부적합 음용의 수질처리는 화학약품, 필터여과, UV살균, O3 (플라즈마)을 이용하는 것이 대표적이나, 화학약품의 경우 2차 오염이나 식품 세척 및 가공에 있어 부적합성의 한계점이 있다. 필터여과의 대표적인 RO필터의 경우 약 50% 순손실이 발생하고, UV 살균의 경우 UV에 의한 사용관리자의 위험 및 장비의 광부식 문제, O3 의 경우 고압전류 사용에 따른 위험성 등의 한계점이 나타나고 있다. 지하수 수질정화를 위한 광유도 활성산소(1O2, ·O-2)는 광감응제에 가시광의 빛 조사를 통해 생성되는 활성산소로의 에너지 및 전자 전이가 동시 진행되어 단일항 산소(1O2)와 슈퍼옥사이드 이온(·O-2)을 생성하게 된다. 생성된 활성산소는 유해미생물 또는 유기화학물과 개열, 제거, 치환 반응 등을 통해 미생물사멸 및 유해화학물질들이 분해 가능하다. 이를 이용한 지하수 유해미생물 사멸기술, 장비, 실시간 지하수의 분석기술 및 정수처리, 지하수 물순환 시스템 개발뿐만 아니라 지하수 음용수 및 오염개선, 지하수 기저유출에 의한 오염원 저감으로부터 지류·지천, 하천 본류 수질개선 등의 대상지역에 활용 가능하다. 또한 광유도 활성산소는 기존 상수도 수처리에 있어 오존(O3) 처리와 이산화티탄을 이용한 AOP과정을 단일처리 공정으로, 기존 O3 의 특성상 확산 거리가 매우 길어 사람을 포함한 생체 내에 유입 시 다양한 부작용 발생과 O3 차폐시설 요구의 문제점 극복의 대안으로 환경 및 인체에 무해한 광유도 활성산소 시스템을 적극적으로 도입 및 적용해야 한다. 본 연구 목적은 정류상태 흡광분광기술을 이용한 실시간 수질 모니터링과 광유도 활성산소를 이용한 유해 미생물의 멸균효능 및 지하수 수질관리 기술로의 적용 가능성을 제시하고자 한다.

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Photocatalytic disinfection of indoor suspended microorganisms (Escherichia coli and Bacillus subtilis spore) with ultraviolet light (광촉매와 UVA에 의한 실내 부유 미생물(E. coli 및 Bacillus. subtilis sp.) 살균 제거 연구)

  • Yoon, Young H.;Nam, Sook-Hyun;Joo, Jin-Chul;Ahn, Ho-Sang
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.2
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    • pp.1204-1210
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    • 2014
  • New control methods are proposed for indoor air quality by removing fine airborne dust-particles. As suspended fine dust-particles contain inorganic dust as well as fine organic bacteria, studies for simultaneous control of these contaminants are required. In this study, photocatalytic disinfection of indoor suspended microorganisms such as E. coli and Bacillus subtilis is performed by three types of photocatalysts with UVA irradiation. The UVA irradiation strength was controlled to the minimum $3{\mu}W/cm^2$, and ZnO, $TiO_2$, and ZnO/Laponite ball were used as the catalysts. The results indicate that E. coli was removed over 80 % after about 2 hours of reaction with UVA and all three types of photocatalysts, whereas only with UVA, around 50 % E. coli removal was obtained. Among the catalysts, ZnO/Laponite composite ball was found to have similar sterilizing capacity to $TiO_2$. However, in case of B. subtilis, which has thick cell wall in its spore state, disinfection was not effective under the low UVA irradiation condition, even with the catalysts. Further studies need to figure out the optimal UVA irradiation ranges as well as photocatalysts doses to control airborne dust, to provide healthy clean air environment.

Development of an Ultra-Violet Lamp and a Ballast for Ship's Ballast Water Treatment (선박평형수 처리용 자외선 램프 및 안정기 개발)

  • Cheon, Sang-Gyu;Park, Dae-Won;Kil, Gyung-Suk
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.5
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    • pp.675-681
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    • 2011
  • In this paper, we dealt with the design and fabrication of a medium pressure ultra-violet (UV) lamp and a magnetic ballast which are main components for ballast water treatment systems (BWTS). To acquire an optimal discharge condition of UV lamp, electrical and optical characteristics depending on the argon gas volume and the amount of mercury were experimentally analyzed. Rated voltage, current and power consumption of a prototype lamp were 490 [V], 8.6 [A] and 4.0 [kW], respectively. UV intensity of the lamp was 15 [%] higher than that of an equivalent lamp which is used in a BWTS. The magnetic ballast was designed in a UI core type through theoretical analysis and simulation. The open voltage and the rated power consumption of the ballast were 920 [V] and 8.5 [kVA] respectively. The disinfection efficacy which is carried out in a BWTS equipped with the UV lamp and magnetic ballast was over 99.99 [%], and this satisfy the IMO regulations.