• Proceedings of the Korean Information Science Society Conference
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• 1998.10a
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• pp.74-76
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• 1998

본 논문에서는 유닉스 파일 시스템에서의 버퍼캐쉬 크기에 따라 사용자 데이터와 메타데이터의 버퍼 캐쉬 히트율을 분석하였다. 그리고 메타 데이터가 유닉스 운영체제 파일 시스템의 성능에 미치는 영향을 분석하고 이를 기반으로 버퍼 캐쉬의 동적 특성과 성능의 장애 요인들을 분석하였다. 유닉스 운영체제에서 사용되는 사용자 데이터와 메타데이터에 대한 버퍼 캐쉬의 동적인 동작을 분석하기 위하여 trace-driven방법을 이용하였으며 이를 위하여 시뮬레이터를 작성.사용하였다. 파일 시스템은 특정 유닉스 버전에 영향을 받지 않기 위해 USF[1]에 기초하였고, 작업부하(workload)로는 Sprite- trace 데이터 중 allspice 서버에서 추출한 데이터를 사용하였다.

• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.625-632
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• 2019

The unified saccharification and fermentation (USF) system was developed for direct production of ethanol from agarose. This system contains an enzymatic saccharification process that uses three types of agarases and a fermentation process by recombinant yeast. The $pGMF{\alpha}-HGN$ plasmid harboring AGAH71 and AGAG1 genes encoding ${\beta}-agarase$ and the NABH558 gene encoding neoagarobiose hydrolase was constructed and transformed into the Saccharomyces cerevisiae 2805 strain. Three secretory agarases were produced by introducing an S. cerevisiae signal sequence, and they efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexose. To directly produce ethanol from agarose, the S. cerevisiae $2805/pGMF{\alpha}-HGN$ strain was cultivated into YP-containing agarose medium at $40^{\circ}C$ for 48 h (for saccharification) and then $30^{\circ}C$ for 72 h (for fermentation). During the united cultivation process for 120 h, a maximum of 1.97 g/l ethanol from 10 g/l agarose was produced. This is the first report on a single process containing enzymatic saccharification and fermentation for direct production of ethanol without chemical liquefaction (pretreatment) of agarose.

• Journal of Information Processing Systems
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• v.15 no.4
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• pp.957-966
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• 2019

This paper studies a novel approach to natural gait cycles based gait recognition via kernel Fisher discriminant analysis (KFDA), which can effectively calculate the features from gait sequences and accelerate the recognition process. The proposed approach firstly extracts the gait silhouettes through moving object detection and segmentation from each gait videos. Secondly, gait energy images (GEIs) are calculated for each gait videos, and used as gait features. Thirdly, KFDA method is used to refine the extracted gait features, and low-dimensional feature vectors for each gait videos can be got. The last is the nearest neighbor classifier is applied to classify. The proposed method is evaluated on the CASIA and USF gait databases, and the results show that our proposed algorithm can get better recognition effect than other existing algorithms.

• The Journal of Information Systems
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• v.22 no.1
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• pp.201-223
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• 2013

모바일뱅킹은 빠르게 성장하고있으며 은행서비스의 많은 부분이 모바일뱅킹에서 제공되고 있다. 하지만, 모바일뱅킹을 사용자들이 수용하는데 개인의 혁신성이 어떠한 영향을 미치는지에 대한 연구는 많이 이루어지지 않고 있다. 불행히도 모바일뱅킹은 그 특성상 제한된 화면을 사용자들이 사용하기가 용이하지 않다. 본 연구는 개인혁신성(PI)의 관점에서 모바일뱅킹을 수용하는데 영향을 미치는 요인을 밝히고 자하였다. 특히 다른 정보시스템에서 많이 사용된 지각된 유용성(USF) 및 사용의 용이성(EOU) 변수뿐만 아니라 지각된 즐거움(ENJ)의 요인을 추가하여 모바일뱅킹 수용에서 영향요인을 분석하고자 하였다. 또한, 본 연구에서는 336명의 모바일뱅킹의 사용자들에게 설문을 실시하였으며, 응답자들을 두 그룹(개인 혁신성이 높은 그룹과 낮은 그룹)으로 분류하여 구조방정식모형에서 집단간차이를 분석하였다. 본 연구결과 개인 혁신성(PI) 모바일뱅킹 수용에 영향을 미치는 것으로 분석되었다. 세부적으로 분석해 보면 개인혁신성이 높은 그룹이 낮은 그룹 보다 더 많은 영향을 받는 것으로 분석되었다. 특히, 사용의도와 사용의 유용성 그리고 지각된 즐거움 사이의 경로계수 값의 크기가 개인혁신성이 높은 그룹이 낮은 그룹보다 높았다. 여기서 지각된 즐거움의 경우 개인 혁신성이 높은 그룹이 낮은 그룹 보다 더 많은 영향을 받는 것으로 분석되었다. 이러한 연구의 결과는 모바일뱅킹 서비스의 성장과 확대에 실무적으로 사용할 수 있을 것으로 분석된다.

• BMB Reports
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• v.30 no.3
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• pp.205-210
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• 1997

p53 tumor suppressor plays an important role in the regulation of cellular proliferation. To identify proteins regulating the expression of p53 in rat liver, we analyzed p53 promoter by electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay. We found that a protein binds the sequence CACGTG, bHLH consensus sequence in rat p53 promoter. Southwestern blotting analysis with oligonucleotides containing this sequence shows that the molecular weight of the protein is 100 kDa. This size is not compatible with the bHLH family such as USF or c-Myc/Max which is known to regulate the expression of the human and mouse p53 gene. Therefore this 100 kDa protein may be a new protein regulating basal transcription of rat p53. We purified this 100 kDa protein through sequence-specific DNA affinity chromatogaphy.

• Fisheries and Aquatic Sciences
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• v.12 no.2
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• pp.90-97
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• 2009

The cytoskeletal $\beta$-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream $\beta$-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream $\beta$-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the $\beta$-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream $\beta$-actin gene was much more homologous with those of teleostean $\beta$-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream $\beta$-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.

• Proceedings of the Korea Society of Poultry Science Conference
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• 1999.11a
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• pp.34-50
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• 1999

A cDNA encoding chicken interferon-gamma (chIFN-${\gamma}$) was amplified from P34, a CD4$^{+}$ T-cell hybridoma by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pUC18. THe sequences of cloned PCR products were determined to confirm the correct cloning. Using this cDNA as probe, chicken genomic library from White Leghorn spleen was screened. Phage clones harboring chicken interferon-gamma (chIFN-${\gamma}$) were isolated and their genomic structure elucidated. The chIFN-${\gamma}$ contains 4 exons and 3 introns spanning over 14 kb, and follows the GT/AG rule for correct splicing at the exon/intron boundaries. The four exons encode 41, 26, 57 and 40 amino acids, respectively, suggesting that the overall structure of IFN-${\gamma}$ is evolutionairly conserved in mammalian and avian species. The 5’-untranslated region and signal sequences are located in exon 1. Several AT-rich sequences located in the fourth exon may indicate a role in mRNA turnover. The 5’-flanking region contains sequences homologous to the potential binding sites for the mammalian transcription factors, activator protein-1(AP-1) activator protein-2(AP-2) cAMP-response element binding protein(CREB), activating transcription factor(ATF), GATA-binding fator(GATA), upstream stimulating factor(USF), This suggests that the mechanisms underlying transcriptional regulation of chicken and mammalian IFN-${\gamma}$ genes may be similar.r.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.28 no.8
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• pp.106-112
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• 2014

This paper presents the analysis of connected operation of photo voltaic source and Li energy storage system. The micro-grid has been installed and operated for several years at the campus of USF and has been a role of test bed. Photo voltaic source has been strongly influenced by the location, weather and climate of a installed area and Li battery is connected directly to the photo voltaic source to compensate for the limitations. The Li battery is operated to supply power output to the grid by the charging or discharging mode based on the average power output of the PV source which is calculated from monitored data for several years. The load of the PV and Li battery system is operated as a severe loading condition and the operating characteristics of PV source and Li battery are analyzed in detail. In connected operations of PV and Li battery to power system, the PV and Li battery is operated to supply constant power during only day time or peak time to increase load shedding ratio and efficient usage of generation sources in power system.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.7
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• pp.4692-4697
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• 2014

A micro-grid including photovoltaic source and Li battery has been installed and operated for several years at the campus of USF and been used as a test bed. Photovoltaic power source has been strongly influenced by the location, weather and climate of the installed area. To compensate for the uncertainty of photovoltaic source's power output, a Li battery is connected directly to the photovoltaic source and supplies electric power to the grid. The Li battery is operated to supply power output to the grid according to the charging or discharging mode of the battery based on the average power output of the photovoltaic source, which is calculated from the monitored data for several years. The grid of the photovoltaic and Li battery system is operated as a severe loading condition and the operating characteristics of PV source and Li battery cells are analyzed in detail.

• Journal of The Korea Institute of Healthcare Architecture
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• v.26 no.2
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• pp.7-17
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• 2020

Purpose: The purpose of this study is to identify the operational status and current management of Sub-Health Centers in Limpio, Paraguay. Second, understanding the use of Sub-Health Centers and Perceptions of Sub-Health Centers in Limpio, Paraguay. Third, Providing policy implications for strengthening the Health Delivery System in Paraguay. Methods: The survey of the current status of sub-health centers in Limpio was conducted with observation and interview. Utilization of Sub-Health centers was analyzed in the 2018 Paraguay Community Health Survey. A face to face interview was conducted to complete a questionnaire and 831 samples were collected for the study. Results: In order to perform the normal function of the sub-health center, it will be necessary to allocate manpower that meets the standard. A common problem with sub-health centers in Limpio is that they have an environment vulnerable to rain. Currently, there are no health promotion and communicable disease management programs in sub-health center. Satisfaction of users about treatment, equipment, medicines and cleanleness of rooms. Implications: First, it is necessary to allocate human resources and organize spaces according to the standard. Second, there was a problem caused by moisture, and continuous maintenance and repair are required. Third, water and sewage related facilities must be safely improved to prevent contamination of groundwater. Forth, it is necessary to implement a program that fits the role of the sub-health center. Fifth, it is necessary to form a health delivery system considering the accessibility of residents. Finally, it is necessary to discuss the location of sub-health center considering travel time of Limpio residents.