• Archives of Pharmacal Research
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• v.10 no.3
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• pp.165-168
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• 1987

The effect of scoparone on UDP glucuronyltransfearase in mouse hepatic microsomes was studied. After transment with scoparone, hepatic microsomal UDP glucuronyltransferase activity was increased with dose-dependent manner as compared to control. The V$_max$ value (control = 23.2 n moles/mg protein/min, scoparone = 31.2 n moles/ mg protein /min) without affecting the $K_m$ value (414 $\mu$M) for p-nitrophenol was increased by the scoparone treatment, and the pattern of kinetic studies for UDP-glucuronic acid was also similar to those of p-nitrophenol. Whereas, the hepatic microsomal UDP glucuronyl-transferase was not changed by the addition of scoparone in vitro. The results obtained suggest that the characteristics of increase in the enzyme activity may include induction of enzyme proteins.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11b
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• pp.1249-1252
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• 2002

클러스터와 같은 네트워크 컴퓨팅 환경에서는 신속하고 신뢰성이 보장되는 데이터 전송이 요구된다. 신뢰성 보장을 위해서 일반적으로 사용되는 전송 프로토콜은 TCP 이다. 그러나 클러스터의 하부 네트워크로서 많이 사용되는 Myrinet 은 cut-through 스위칭 방식을 기반으로 하기 때문에 네트워크 혼잡(congestion)이 발생하지 않는다. 따라서 TCP 의 혼잡 제어(congestion control) 등과 같은 루틴들은 Myrinet 상에서 불필요한 오버헤드를 발생시킨다. 본 논문은 Myrinet 네트워크에서 흐름 제어(flow control)만으로도 신뢰성을 보장할 수 있음을 보이고 TCP 보다 오버헤드가 적은 UDP에 흐름 제어를 구현한 RUM(Reliable UDP on Myrinet)을 제안한다. 성능 측정 결과, RUM 은 신뢰성을 보장함과 동시에 TCP 보다 최대 34% 더 높은 처리량(throughput)을 보이며, UDP 와 비슷한 낮은 단방향 지연시간(one-way latency)을 보장함을 알 수 있다.

• Journal of KIISE:Computing Practices and Letters
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• v.13 no.3
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• pp.153-164
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• 2007

The Access Grid (AG) provides collaboration environments over the IP multicast networks by enabling efficient exchange of multimedia contents among remote users; however, since lots of current networks are still multicast-disabled, it is not easy to deploy this multicast-based multi-party AG. For this problem, the AG provides multicast bridges as a solution by putting a relay server into the multicast networks. Multicast-disabled clients make UDP connections with this relay server and receive forwarded multicast traffics in unicast UDP packets. This solution is facing several limitations since it requires duplicate forwarding of the same packet for each unicast peer. Thus, in this paper, we propose an alternate solution for the multicast connectivity problem of the AG based on the UMTP (UDP multicast tunneling protocol). By taking advantage of flexibilities of UMTP, the proposed solution is designed to improve the efficiency of network and system utilization, to allow reuse of multicast-based AG applications without modification, and to partially address the NAT/firewall traversal issues. To verify the feasibility of proposed solution, we have implemented a prototype AG connectivity tool based on the UMTP, named as the AG Connector.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1197-1203
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• 2001

This study was conducted to examine the effects of dietary xylooligosaccharide on UDP-glucuronyl transferase (UDP-GTase) activity and excretion of fecal sterols in rat fed high cholesterol diet. Sprague-Dawley male rats weighing 100$\pm$10 g were randomly divided into five groups, one with normal diet and four with high cholesterol diets containing 1% cholesterol (w/w). The high groups with cholesterol diet groups were classified into xylooligosaccharide free diet (C), 5% xylooligosaccharide diet (C5XO), 10% xylooligosaccharide diet (C10XO), and 15% xylooligosaccharide diet (C15XO) group according to the five groups of dietary xylooligosaccharide by weights. The experimental diets were fed ad libidum for 4 weeks. Fecal weights were increased 86% by xylooligosaccharide. Fecal total lipid contents including fecal neutral and acidic sterols in xylooligosaccharide groups were significantly higher than those of the normal and C groups, and especially that of C10XO group was the highest among all experimental groups. Activity of UDP-glucuronyl transferase (UDP-GTase) in liver in C group was 35% higher than that of normal group and the activities in C5XO, C10XO and C15XO groups were 15%, 41%, and 21% higher than in C group, respectively. Fecal bile acid excretions per day were increased 3.1, 3.6 and, 2.8 folds in C5XO, C10XO, and C15XO groups, respectively, compared with that of C group. Contents of neutral sterol, coprostanol, and coprostanone were higher in xylooligosaccharide groups than in C group. These results suggest that dietary xylooligosaccharide may act as potential substitute for a dietary fiber capable of improving a gastrointestinal function and lipid metabolism.

• Journal of KIISE:Information Networking
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• v.32 no.6
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• pp.732-744
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• 2005

Previous works for the AE(Assured Forwarding) service in the Diffserv network have no sufficient consideration on the fairness of bandwidth share based on the target rate and the effect or RTT and UDP. Also Previous works act like Best-effort service in the UPN(under-Provisioned Network) condition. In this paper, in order to solve these problems, we propose the PFDSA(Proportionally Fair Differentiated Service Architecture) composed of tmTRA3CM(tcp-microflow based Target rate and an Aware Three color Marking), um3CM(udp-microflow based Three color Marker), TRBD(Target Rate Based Dropper), and target rate adjusting function. In the results of comparing the performance among existing mechanisms and the PFDSA, the PFDSA was able to mitigate the RTT and UDP effect better than the former. The PFDSA was shown to provide good performance for transmission rates proportional to various target rates in the UPN condition.

• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1547-1552
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• 2009

Sphingomonas chungbukensis DJ77 has the ability to produce large quantities of an extracellular polysaccharide that can be used as a gelling agent in the food and pharmaceutical industries. We identified, cloned and expressed the UDP-glucose dehydrogenase gene of S. chungbukensis DJ77, and characterized the resulting protein. The purified UDP-glucose dehydrogenase (UGDH), which catalyzes the reversible conversion of UDP-glucose to UDPglucuronic acid, formed a homodimer and the mass of the monomer was estimated to be 46 kDa. Kinetic analysis at the optimal pH of 8.5 indicated that the $K_m\;and\;V_{max}$ for UDP-glucose were 0.18 mM and 1.59 mM/min/mg, respectively. Inhibition assays showed that UDP-glucuronic acid strongly inhibits UGDH. Site-directed mutagenesis was performed on Gly9, Gly12 Thr127, Cys264, and Lys267. Substitutions of Cys264 with Ala and of Lys267 with Asp resulted in complete loss of enzymatic activity, suggesting that Cys264 and Lys267 are essential for the catalytic activity of UGDH.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.10a
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• pp.576-578
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• 2016

Various smart home IoT devices and services while the Internet of things is the development has been provided. The core of the smart home IoT service is that user control the device via the Internet communication. Communication of IoT devices, because most with an IP address within the private network, there is a difficulty in the remote control to control access from outside the network. Any of the methods for remote control, to determine the IP address of each other, there is a UDP hole punching for communication. To ensure the data communication success rate closed to 100%, the UDP hole punching must undergo a process of three stages in some cases. In this paper, to provide a system for managing the remote control policy based on the CEP in order to omit the unnecessary steps on the remote control of IoT devices using UDP hole punching.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.23 no.3
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• pp.59-64
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• 2023

Fire and disaster broadcasting systems are divided into analog, digital, and network-based digital public address systems, and important specifications in network-based digital public address systems are low-latency audio, high sampling rate, and multi-channel input and output. In the past, it has been widely used to the AoE method for distinguishing based on the MAC address of the data link layer. However, this method has a problem of increasing complexity and cost. This proposal is an AoIP/UDP method, which allows communication to be easily distinguished by IP address without the need for a separate redundant network, so that the network can be freely used and configured, and cost can be reduced by reducing complexity. After implementing the AoIP/UDP method, the experimental results showed that the cost was improved with the equivalent performance with 2.66ms latency.

• Animal Bioscience
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• v.36 no.10
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• pp.1604-1611
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• 2023

Objective: The aim of this study was to investigate the protective effect of wheat phytase as a structural decomposer of inflammatory nucleotides, extracellular adenosine triphosphate (ATP), and uridine diphosphate (UDP) on HT-29 cells. Methods: Phosphatase activities of wheat phytase against ATP and UDP was investigated in the presence or absence of inhibitors such as L-phenylalanine and L-homoarginine using a Pi Color Lock gold phosphate detection kit. Viability of HT-29 cells exposed to intact- or dephosphorylated-nucleotides was analyzed with an EZ-CYTOX kit. Secretion levels of pro-inflammatory cytokines (IL-6 and IL-8) in HT-29 cells exposed to substrate treated with or without wheat phytase were measured with enzyme-linked immunosorbent assay kits. Activation of caspase-3 in HT-29 cells treated with intact ATP or dephosphorylated-ATP was investigated using a colorimetric assay kit. Results: Wheat phytase dephosphorylated both nucleotides, ATP and UDP, in a dose-dependent manner. Regardless of the presence or absence of enzyme inhibitors (L-phenylalanine and L-homoarginine), wheat phytase dephosphorylated UDP. Only L-phenylalanine inhibited the dephosphorylation of ATP by wheat phytase. However, the level of inhibition was less than 10%. Wheat phytase significantly enhanced the viability of HT-29 cells against ATP- and UDP-induced cytotoxicity. Interleukin (IL)-8 released from HT-29 cells with nucleotides dephosphorylated by wheat phytase was higher than that released from HT-29 cells with intact nucleotides. Moreover, the release of IL-6 was strongly induced from HT-29 cells with UDP dephosphorylated by wheat phytase. HT-29 cells with ATP degraded by wheat phytase showed significantly (13%) lower activity of caspase-3 than HT-29 cells with intact ATP. Conclusion: Wheat phytase can be a candidate for veterinary medicine to prevent cell death in animals. In this context, wheat phytase beyond its nutritional aspects might be a novel and promising tool for promoting growth and function of intestinal epithelial cells under luminal ATP and UDP surge in the gut.

• Archives of Pharmacal Research
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• v.20 no.5
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• pp.459-464
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• 1997

The human liver cDNA clone UDPGTh2, encoding a liver UDP-glucuronosyltransferase (UDPGT) was isolated from a .gamma. gt 11 cDNA library by hybridization to mouse transferase cDNA clone, UDPGTm1. UDPGTh2 encoded a 529 amino acid protein with an amino terminus membrane-insertion signal peptide and a carboxyl terminus membrane-spanning region. There were three potential asparagine-linked glycosylation sites at residues 67, 68, and 315. In order to obtain UDPGTh2 protein encoded from cloned human liver UDP-glucuronosyltransferase cDNA, the clone was inserted into the pSVL vector (pUDPGTh2) and expressed in COS 1 cells. The presence of a transferase with Mr-52,000 in transfected cells cultured in the presence of $[^{35}S]$ methionine was shown by immunocomplexed products with goat antimouse transferase IgG and protein A-Sepharose and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. The expressed UDPGT was a glycoprotein as indicated by electrophoretic mobility shift in Mr-3,000-4,000 when expressed in the presence of tunicamycin. The extent of glycosylation was difficult to assess, although one could assume that glycosyl structures incorporated at the level of endoplasmic reticulum were always the core oligosaccharides. Thus, it is likely that at least two moieties inserted can account for the shift of Mr-3,000-4,000. This study demonstrates the cDNA and deduced amino acid sequence of human liver UDP-glucuronosyltransferase cDNA, UDPGTh2.