• Title/Summary/Keyword: UBC

Search Result 154, Processing Time 0.033 seconds

Validation of Reference Genes for Quantifying Changes in Physiological Gene Expression in Apple Tree under Cold Stress and Virus Infection (저온과 바이러스 감염에 노출된 사과나무의 생리적 유전자 정량 측정용 유전자들의 발현 분석 및 검증)

  • Yoon, Ju-Yeon;Jeong, Jae-Hoon;Choi, Seung-Kook
    • Research in Plant Disease
    • /
    • v.26 no.3
    • /
    • pp.144-158
    • /
    • 2020
  • Quantitative reverse transcription PCR is used for gene expression analysis as the accurate and sensitive method. To analyze quantification of gene expression changes in apple plants, 10 housekeeping genes (ACT, CKL, EF-1α, GAPDH, MDH, PDI, THFs, UBC, UBC10, and WD40) were evaluated for their stability of expression during infection by Apple stem grooving virus (ASGV) or in cold-stress apple plant buds. Five reference-gene validation programs were used to establish the order of the most stable genes for ASGV as CKL>THFs>GAPDH>ACT, and the least stable genes WD40CKL>UBC10, and the least stable genes were ACT

A Study on the Application of UBC3D-PLM for Soil Liquefaction Analysis (액상화 해석을 위한 UBC3D-PLM의 적용성에 관한 연구)

  • Park, Eon-Sang;Kim, Byung-Il
    • Journal of the Korean Geosynthetics Society
    • /
    • v.21 no.1
    • /
    • pp.1-10
    • /
    • 2022
  • In this study, a model parameter evaluation method using relative density was proposed to utilize applicable UBC3D-PLM for liquefaction behavior. In addition, dynamic effective stress analysis, that is, liquefaction analysis, was performed on the case of the liquefaction occurrence region where acceleration and pore water pressure were measured, and compared with the actual measurement and the existing Finn analysis results. Through this study, it was found that the proposed method can easily evaluate the necessary parameters required by the related model and predict the pore water pressure behavior in the region where liquefaction occurs. In addition, in the case of the study area, both measurements and numerical analysis showed that liquefaction occurred when a certain amount of time elapsed after the earthquake acceleration reached the maximum value. In the case of UBC3D-PLM applied in this study, the excess pore water pressure behavior similar to the actual measurement was predicted, and the occurrence of liquefaction was evaluated in the same way as the actual measurement. In particular, although the excess pore water pressure in the sand layer was greater, the phenomenon in which liquefaction occurred in the silt layer was accurately realized. It is expected that the proposed model parameter evaluation method and finite element analysis applying UBC3D-PLM can be used to select the liquefaction reinforcement region in the future seismic design and reinforcement by evaluating the liquefaction occurrence region similarly to the real one.

Discrimination of Phlomidis Radix and Dipsaci Radix using the Random Amplified Polymorphic DNA Analysis (Random Amplified Polymorphic DNA 분석을 이용한 한속단과 천속단의 감별)

  • Lee, Mi-Young;Ryuk, Jin-Ah;Kim, Hong-Jun;Kim, Young-Hwa;Chae, Byoung-Chan;Ko, Byoung-Seob
    • Korean Journal of Oriental Medicine
    • /
    • v.13 no.1 s.19
    • /
    • pp.147-152
    • /
    • 2007
  • As a result to amplifying 12 samples of 'Sok-dan' through an random amplified polymorphic DNA (RAPD) method using eighteen DEC and URP primers, distinct band forms enabling discrimination of Phlomus umbrosa and Dipsacus asperoides were observable in the UBC 320 primer, UBC 367 primer, UBC 385 primer, UBC 414 primer, UBC 423 primer, URP 3 primer, URP 5 primer and URP 9 primer. The polymorph result amplified with a random primer was evaluated through Gelcompar II, showing a result dividable into two groups. The divided groups were the dried sample group of Dipsacus asperoides and the group of Phlomis umbrosa. In order to recognize the distinction between Dipsaci Radix types, the genetic variation of 'Sok-dan' produced domestically and imported was evaluated through RAPD, and the potential to distinguish these in forms of dried medicine was identified, presenting a method to authentification of Phlomis umbrosa and Dispacus asperoides.

  • PDF

Pathologic Fracture of Unicameral Bone Cyst (단순 골낭종에서 발생한 병적 골절)

  • Choo, Suk-Kyu;Lee, Hee-Du;Oh, Hyoung-Keun
    • The Journal of the Korean bone and joint tumor society
    • /
    • v.17 no.2
    • /
    • pp.58-64
    • /
    • 2011
  • Purpose: The purpose of this study is to observe unicameral bone cyst (UBC) outcome after the fracture has healed and if there is any identifiable prognostic factors. Materials and Methods: 13 UBC patients with pathologic fracture from 2001 to 2010 were reviewed. The mean follow up were 26 months (3-90 months). There were 11 male and 2 female patients and the mean age of the patients were 10.2 years old (6-16 years). 9 involved proximal humerusand 2 involved humerus shaft and 1 involved proximal femur and 1 involved proximal tibia. The treatment of UBC fracture was conservative cast application to heal the fracture initially, and 1 patient was treated with primary auto bone graft and open reduction with internal fixation. 5 patients were treated with steroid injection during follow up period and 2 patients with auto bone graft. We analyzed the change of UBC during pathologic fracture healing period and prognostic factor about age, the size of UBC, the involvement of physis. Results: The mean duration of the fracture healing was 8.2 months. Complete healing were occurred at 4 patients (31%). No statiscal difference was checked with age about UBC healing (p=0.42). But, more larger size about UBC and more closer to physis, the healing was difficult (p=0.05, p=0.03). Conclusion: While pathologic fracture of UBC was possibly healed, active treatment should be applied especially those cysts that involvescloser area of the physis or large size.

Importance of Dynamic Cue in Silhouette-Based Gait Recognition (실루엣 기반 걸음걸이 인식 방법에서 동적 단서의 중요성)

  • Park Hanhoon;Park Jong-Il
    • Journal of the Institute of Electronics Engineers of Korea SP
    • /
    • v.42 no.3 s.303
    • /
    • pp.23-30
    • /
    • 2005
  • As a human identification technique, gait recognition has recently gained significant attention. Silhouette-based gait recognition is one of the most popular methods. This paper aims to investigate features that determine the style of walking in silhouette-based gait recognition. Gait can be represented using two cues: static(shape) cue and dynamic(motion) cue. Most recently, research results have been reported in the literature that the characteristics of gait are mainly determined by static cue but not affected by dynamic cue. Unlike this, experimental results in this paper verifies that dynamic cue is as important as and in many cases more important than static cue. For experiments, we use two well-blown gait databases: UBC DB and Southampton Small DB. The images of UBC DB correspond to the 'ordinary' style of walking. The images of Southampton Small DB correspond to the 'disguised' (not ordinary by wearing special clothes or bags) style of walking. As results of experiments, the recognition rate was 100% by static cue and $95.2\%$ by dynamic cue for the images of UBC DB. For the images of Southampton Small DB, the recognition rate was $50.0\%$ by static cue and $55.8\%$ by dynamic cue. The risk against correct recognition was 0.91 by static cue and 0.97 by dynamic cue for the images of UBC DB. For the images of Southampton Small DB, the risk was 0.98 by static cue and 0.98 by dynamic cue. Consequently, the characteristics of ordinary gait are mainly determined by static cue but that of disguised gait by dynamic cue.

Association of A/T Rich Microsatellites with Responses to Artificial Selection for Larval Developmental Duration in the Silkworm Bombyx mori

  • Pradeep, Appukuttan Nair Retnabhavan;Awasthi, Arvind Kumar;Urs, Raje Siddaraje
    • Molecules and Cells
    • /
    • v.25 no.4
    • /
    • pp.467-478
    • /
    • 2008
  • Simple sequence repeats (SSRs) and interSSR (ISSR) marker systems were used in this study to reveal genetic changes induced by artificial selection for short/long larval duration in the tropical strain Nistari of the silkworm Bombyx mori. Artificial selection separated longer larval duration (LLD) ($29.428{\pm}0.723days$) and shorter larval duration (SLD) ($22.573{\pm}0.839days$) lines from a base, inbred population of Nistari (larval span of $23.143{\pm}0.35days$). SSR polymorphism was observed between the LLD and SLD lines at one microsatellite locus, Bmsat106 ($CA_7$) and at two loci of 1074 bp and 823 bp generated with the ISSR primer UBC873. Each of these loci was present only in the LLD line. The loci segregated in the third generation of selection and were fixed in opposite directions. In the $F_2$ generation of the $LLD{\times}SLD$ lines, the alleles of Bmsat106 and $UBC873_{1074bp}$ segregated in a 1:1 ratio and the loci were present only in the LLD individuals. $UBC873_{823bp}$ was homozygous. Single factor ANOVA showed a significant association between the segregating loci and longer larval duration. Together, the two alleles contributed to an 18% increase in larval duration. The nucleotide sequences of the $UBC873_{1074bp}$ and $UBC873_{823bp}$ loci had 67% A/T content and consisted of direct, reverse, complementary and palindromic repeats. The repeats appeared to be "nested" (59%) in larger repeats or as clustered elements adjacent to other repeats. Of 203 microsatellites identified, dinucleotides (67.8%) predominated and were rich in A/T and T/A motifs. The sequences of the $UBC873_{1074bp}$ and $UBC873_{823bp}$ loci showed similarity (E = 0.0) to contigs located in Scaffold 010774 and Scaffold 000139, respectively, of the B. mori genome. BLASTN analysis of the $UBC873_{1074bp}$ sequence showed significant homology of (nt.) 45-122 with upstream region of three exons from Bombyx. The complete sequence of this locus showed ~49% nucleotide conservation with transposon 412 of Drosophila melanogaster and the Ikirara insertions of Anopheles gambiae. The A + T richness and lack of coding potential of these small loci, and their absence in the SLD line, reflect the active process of genetic change associated with the switch to short larval duration as an adaptation to the tropics.

Primers for typing Listeria spp. using Random Amplified Polymorphic DNA (RAPD) ANalysis (Listeria spp.의 RAPD typing을 위한 Primer의 분리력 비교)

  • 임형근;홍종해;박경진;최원상
    • Journal of Food Hygiene and Safety
    • /
    • v.18 no.2
    • /
    • pp.67-72
    • /
    • 2003
  • Random amplified Polymorphic DNA (RAPD) analysis Is based on the amplification of random DNA segment using a single arbitrary primer. Polymorphic DNA patterns identified by this method can be used for typing Listeria monocytogenes. To select the primers for RAPD typing Listeria spp., the performance of 31 primers were compared by analyzing 13 Listeria spp. reference strains. Reproducible electrophoresis patterns were obtained. Among 31 primers, 6 primers (primer 6, HLWL74, UBC155, UBC127, Lis5, Lis11) showed better differentiation, when discrimination index, band clarity, band number, difficulty of band scoring were considered than the others. These primers will be useful far typing Listeria spp. in the future. Currently, we are under investigation for the RAPD typing of contaminated L. monocytogenes for the risk analysis of pork processing plant using these primers.

Necessity and adequacy of near-source factors for seismically isolated buildings

  • Saifullah, Muhammad Khalid;Alhan, Cenk
    • Earthquakes and Structures
    • /
    • v.12 no.1
    • /
    • pp.91-108
    • /
    • 2017
  • Superstructures and isolation systems of seismically isolated buildings located close to active faults may observe increased seismic demands resulting from long-period and high-amplitude velocity and displacement pulses existent in near-fault ground motions as their fundamental periods may be close to or coincident with these near-fault pulse periods. In order to take these effects into account, the 1997 Uniform Building Code (UBC97) has specified near-source factors that scale up the design spectrum depending on the closest distance to the fault, the soil type at the site, and the properties of the seismic source. Although UBC97 has been superseded by the 2015 International Building Code in the U.S.A., UBC97 near-source factors are still frequently referred in the design of seismically isolated buildings around the world. Therefore it is deemed necessary and thus set as the aim of this study to assess the necessity and the adequacy of near-source factors for seismically isolated buildings. Benchmark buildings of different heights with isolation systems of different properties are used in comparing seismic responses obtained via time history analyses using a large number of historical earthquakes with those obtained from spectral analyses using the amplified spectrums established through UBC97 near-source factors. Results show that near-source factors are necessary but inadequate for superstructure responses and somewhat unconservative for base displacement response.

The Development of High Performance Flame Stability(HPFS) Tangential Coal-Fired Burner (고효율 화염 안정형 접선식 석탄 버너 개발)

  • Kim, Hyeok-Pill;Kim, Sang-Hyeun;Kim, Hyuk-Je;Song, Si-Hong
    • Journal of the Korean Society of Combustion
    • /
    • v.11 no.2
    • /
    • pp.1-6
    • /
    • 2006
  • This report presents a study of the development of an advanced coal nozzle used in burners to reduce unburned carbon (UBC) in a tangential coal-fired boiler. To understand the mechanism of UBC reduction, experiments using conventional burners were carried out to evaluate the effects of air injection velocity, coal fineness and over fired air (OFA) on combustion efficiency. It was confirmed that ignition of pulverized coal particles close to the burner is helpful toward the complete burn of residual carbon in fly ash. These efforts indicated the additional results that UBC was strongly dependent on the primary air velocity and coal fineness; especially that UBC dramatically decreased when the weight fraction of pulverized coal under $75{\mu}m$ was over 85 %. New coal nozzles, modified from conventional nozzles, were prepared and tested to improve the combustion efficiency. Some of these nozzles offered relatively lower unburned carbon than those of conventional burners and are referred to as HPFS (High Performance Flame Stability) coal nozzles.

  • PDF