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Strain Improvement of Aspergillus oryzae for Increasing Productivity of a Proteolytic Enzyme. (고활성 단백질분해효소 생산균주의 개발을 위한 Aspergillus oryzae의 원형질체 융합에 의한 변이)

  • 김두상;김형락;남택정;변재형
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.490-496
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    • 1998
  • Aspergillus oryzae producing high proteolytic enzyme was isolated from soybean koji and named tentatively A. oryzae O-1. A. oryzae U-1 was obtained by mutation of A. oryzae O-1 with ultraviolet (UV)-irradiation and produced 14 times higher pretense activity compared with A. oryzae O-1. A. oryzae E-1 was acquired by treatment of A. oryzae U-1 with 0.5 M ethylmethanesulfonate (EMS) for 6 min at 3$0^{\circ}C$ and produced 39 times higher proteolytic activity than A. oryzae O-1. With protoplast fusion between A. oryzae O-1 and A. oryzee E-1 in the presence of polyethylenegylcol (PEG)-CaCl$_2$, proteolytic activity was increased to 82 times compared to A. oryzae O-1, and the fusant was named A. oryzae PF. The activities of the cultures containing proteolytic enzymes produced by the strains were determined to be 0.23 U/$m\ell$ for A. oryzae O-1, 3.29 U/$m\ell$ for A. oryzae U-1, 8.91 U/$m\ell$ for A. oryzae E-1, and 19.0 U/$m\ell$ for A. oryzae PF.

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Fatigue Behavior of the U-Notched Specimens of Structural Carbin Steel under the two Level Stressing(I) (2段階 應力下에서 U - 노치 를 갖는 構造용 炭素鋼材 의 피勞擧動 I)

  • 송삼홍;원시태
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.6 no.2
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    • pp.107-112
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    • 1982
  • In order to analyze and investigate in the fatigue behavior of the notched specimens under the two level stressing, the U-notched specimens of structural carbon steel which is generally used is prepared. The obtained results are summarized as follows; (1) The fatigue limit of the U-notched specimens is lower than that of the unnotched. The fatifue notch factor (.betha.) of the U-notched specimens is 1.44 for mild steel and 1.52 for harden steel. The notch sensitivity (q) is 0.68 for mild steel and o.8 for harden steel. That is, these facts show that harden steel is more sensitive to the notch than mild steel. (2) The fatigue life time of the U-notched specimens under the overstressing is shorter than under the constant stressing, and the degree of fatigue life time decrease is different to each stress level; the degree for the high stress level is more than for the low stress level. (3) The fatigue life time of the U-notched specimes under the understressing is longer than under the constant stressing, and the degree of fatigue life time increase for the low stress level is more than for the high stress level.

Analysis of needs and providing coping strategies for efficient promotion of the u-City IT Infra Construction (u-City IT 인프라구축의 효율적인 추진을 위한 요구사항 분석 및 대응방안 연구)

  • Kim, Jeong-Uk
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.6
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    • pp.2276-2283
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    • 2010
  • This study investigated the needs of u-City related civil servants upon guideline which is constituted for efficient promotion of the u-City IT infra construction. It utilized explanatory design in mixed method as a research design. In the research result, respondents needed as follows : 1)law and institutions involved in construction processes, 2)information about engineering techniques with general terms in u-City constructions, 3)reference service models. It proposed implications of constructing the new guideline based upon research results.

POST-OPERATIVE SKELETAL STABILITY OF THE MAXILLA TREATED WITH LE FORT I AND U-SHAPED OSTEOTOMIES IN SIMULTANEOUS MAXILLOMANDIBULAR ORTHOGNATHIC SURGERY (양악 악교정 수술에서 르포트 I형과 U-자형 복합 골절단술 후 상악골의 안정성에 관한 임상적 연구)

  • Kim, Min-Keun;Park, Young-Wook
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.31 no.6
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    • pp.485-491
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    • 2009
  • Postoperative skeletal stability was evaluated in combination of Le Fort I and U-shaped osteotomies for superior repositioning of maxilla in bi-maxillary surgeries in 30 consecutive patients. The fifteen patients underwent Le Fort I osteotomy alone and the other fifteen patients underwent Le Fort I and U-shaped osteotomies. In all patients, the maxilla was first osteomized and fixed with absorbable plates system. A bilateral sagittal split ramus osteotomy (BSSRO) of the mandible was then carried out and fixation was performed using absorbable plates. Maxillo-mandibular fixation with rubber ring was used for two weeks post-operatively in all patients. Lateral cephalograms were obtained pre-operatively, 1 day post-operatively, 6 months after surgery. The changes in anterior nasal spine (ANS), point A, upper incisior (U1), and point of maxillary tuberosity (PMT) were examined. The maxillas in the fifteen patients of both examination group were repositioned nearly in their planned positions during surgery and no significant post-operative changes in the examined points of the maxilla were found. These results suggest that a combination of a Le Fort I and U-shaped osteotomy is a useful technique for reliable superior repositioning of the maxilla. The post-operative change in the maxilla using this combination osteotomy was comparatively stable.

SHRIMP U-Pb Zircon Ages of the Haeinsa Granite from Central Part of the Yeongnam Massif (영남육괴 중부에 분포하는 해인사화강암의 SHRIMP U-Pb 저어콘 연대)

  • Kim, Sunwoong;Choi, Jeongyun;Kim, Jin-Seop
    • The Journal of the Petrological Society of Korea
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    • v.25 no.4
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    • pp.401-407
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    • 2016
  • The SHRIMP zircon U-Pb age dating was carried out for the age-unknown Haeinsa Granite located in the middle Yeongnam Massif. SHRIMP zircon U-Pb age determinations of 7 samples from the Haeinsa Granite in Geochang area show two age groups. Ages from 5 samples (M-3-1, H-1, 3, 5, 10) are $192.4{\pm}1.4{\sim}195.5{\pm}1.9Ma$, whereas ages from 2 samples (H-11 and 12) are $187.7{\pm}3.3Ma$ and $188.2{\pm}3.6Ma$, respectively.

A Study on the Ubiquitous Safety Monitoring through Utilizing BIM Technology (BIM기반의 U-안전모니터링시스템)

  • KWON, Chang-Hee
    • Journal of Digital Convergence
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    • v.14 no.6
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    • pp.61-67
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    • 2016
  • This study is the basic direction of the BIM bases ubiquitous safety monitoring. First, studied the background and the purpose of the study, and it's scope and methods. Second, described the concept of BIM and investigated how BIM bases proceed through actual condition of ubiquitous safety monitoring. Third, identified BIM bases ubiquitous safety monitoring which can be applied consistently, Forth, DCP bases ubiquitous safety monitoring which can be applied consistently, Also, Finally, suggested the conclusion and sat the future research by analyzing the study results. It is expected to be worked out a way as a new model ubiquitous safety monitoring which will be combined with a ubiquitous DCP.

The Secondary Succession and Species Diversity at the Burned Area of the Pine Forest (소나무 수림의 산화적지의 이차천이와 종다양성)

  • Kim, Weon
    • The Korean Journal of Ecology
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    • v.12 no.4
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    • pp.285-295
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    • 1989
  • This report is results of an investigation on the secondary succession and species diversity of the burned area. Fifty hectares of pine forest was burned by the crown fire of the spring (April 6) 1986. The results obtained from spring 1989 were summarized as follows: In the burned area , site and unburned area (U), the vascular plant fo 69 kinds, 49 kinds and 24 kinds were lsted respectively. The life form spectrum of burned area was H---e type, which is common type in most burned area and that unburned area, H---e type. The species diversity (H)and evenness index (e) of burned area (, site) were H=2.51, 1.65 and e=0.59, 0.44 and those of unburned area (U) were H=1.28 and e=0.40. The similarity index was the value of 0.594 in - and 0.236 in -U site. The similarity between and site was greater than between site and U site, and that between and U-site, The dominance index (c) of , and U-site was 0.16, 0.39 and 0.42 respectively. The succession degree (DS) of , and U-site was 244.0, 227.6 and 854.4 respectively. Soil pH, available phosphorous, and exchangeable potassium in the burned area were higher than those in the unburned area, and total nitrogen, organic carbon, and C/N ratio in the burned area were higher than those in the unburned area, and organic matter in the burned area was higher than that in the unburned area.

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Korea e-Government Portal for Next Generation u-City (차세대 u-City를 위한 정부 대표포털 구축전략)

  • Cho, Young-Im;Jeong, Hyeong-Chul;Oh, Kang-Tak;Kwak, Hee-Sub
    • Journal of the Korean Institute of Intelligent Systems
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    • v.18 no.6
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    • pp.808-813
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    • 2008
  • u-City is a city for users who are in everywhere as well as everytime. Korea e-government portal as a main e-government portal is a gateway for entering Korea e-government, but it has some problems. This portal has to provide a simple interface according to user's needs, but the current system is too complicated so that this system does not provide information retrieval function according to user's needs. So in this paper, we propose an effective strategy and functions of Korea e-government main portal for effective u-City.

uPetCare : Ubiquitous Pet-Care System using Web2.0 (uPetCare : 웹2.0을 이용한 유비쿼터스 펫 케어 시스템)

  • Park, Jun-Sung;Lee, Gwi-Ro;Cho, Jin-Sung
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.4
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    • pp.260-264
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    • 2009
  • There have been many studies on u-Healthcare system for human using sensor network systems. In this paper, we design and implement a healthcare system for pets called uPetCare(Ubiquitous Pet-Care System) that can manage the status of pet on the web. The main functions of this system are 1) gathering data using sensor network, 2) multi-hop communication in sensor network, 3) data compression and aggregation at sink node, 4) storing data in web server, 5) real-time data monitoring using AJAX, 6) activity recognition of pet.

Flow-induced vibrations of three circular cylinders in an equilateral triangular arrangement subjected to cross-flow

  • Chen, Weilin;Ji, Chunning;Alam, Md. Mahbub;Xu, Dong
    • Wind and Structures
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    • v.29 no.1
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    • pp.43-53
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    • 2019
  • Vortex-induced vibration of three circular cylinders (each of diameter D) in an equilateral triangular arrangement is investigated using the immersed boundary method. The cylinders, with one placed upstream and the other two side-by-side downstream, are free to vibrate in the cross-flow direction. The cylinder center-to-center spacing L is adopted as L/D = 2.0. Other parameters include the Reynolds number Re = 100, mass ratio $m^*=2.0$, reduced velocity $U_r=2{\sim}15$ and damping ratio ${\zeta}=0$. Cylinder vibration responses are dependent on $U_r$ and classified into five regimes, i.e. Regime I ($U_r{\leq}3.2$), Regime II ($3.2<U_r{\leq}5.0$), Regime III ($5.0<U_r{\leq}6.4$), Regime IV ($6.4<U_r{\leq}9.2$) and Regime V ($U_r>9.2$). Different facets of vibration amplitude, hydrodynamic forces, wake patterns and displacement spectra are extracted and presented in detail for each regime.