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Effects of Feeding Different Protein Supplements on Digestibility, Nitrogen Balance and Calcium and Phosphorus Utilization in Sheep

  • Viswanathan, T.V.;Fontenot, J.P.
    • Asian-Australasian Journal of Animal Sciences
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    • 제22권5호
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    • pp.643-650
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    • 2009
  • Two metabolism trials were conducted with 24 wether lambs to investigate the effects of feeding crab meal and other protein supplements on N utilization, digestibility and Ca and P balance in sheep. The lambs (avg. BW, 25 kg) were randomly allotted to eight diets in each of two trials. The supplements were: i) none, negative control (NC); ii) soybean meal (SBM), control; iii) supplement based on industrial byproducts of both plant and animal origin (IPA); iv) experimental supplement based on byproducts of animal origin (ESA); v) hydrolyzed supplement No 4. (HESA); vi) commercial supplement based on animal protein (CS), $Pro-Lak^{(R)}$ vii) crab meal (CM); and viii) urea (U). The supplements supplied 33% of the total dietary N (CP, 9.8%; DM basis). Lambs fed the NC diet had lower (p<0.05) DM and OM digestibility. Lower (p<0.05) apparent absorption of N was recorded for the lambs fed the HESA and NC diets. Sheep fed CM had lower Ca absorption compared to SBM. Highest (p<0.05) P absorption was observed for lambs fed CS and CM and lowest for U and NC diets. Sheep fed CM had higher (p<0.05) total VFA concentration (65.7 ${\mu}mol/ml$), compared to those fed ESA, CS, and NC diets (47.3, 49.8, and 49.5 ${\mu}mol/ml$, respectively). Highest (p<0.05) ruminal $NH_3$ N (29.6 mg/dl) was observed in lambs fed the U diet, while those fed the NC diet had the lowest (p<0.05) average value (7.66 mg/dl). Lambs fed the U diet had the highest (p<0.05) blood urea N (10.67 mg/dl). The present study showed that N utilization of diets supplemented with experimental supplements based on feather meal and blood meal; commercial supplement based on animal protein, $Prolak^{(R)}$ supplement based on plant protein and blood meal; and crab meal are comparable with that of soybean meal.

SOME CLASSES OF MULTIVALENT FUNCTIONS WITH NEGATIVE COEFFICIENTS I

  • AUOF, M.K.;DARWISH, H.E.
    • Honam Mathematical Journal
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    • 제16권1호
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    • pp.119-135
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    • 1994
  • Let $Q_{n+p-1}(\alpha)$ denote the- dass of functions $$f(z)=z^{P}-\sum_{n=0}^\infty{a_{(p+k)}z^{p+k}$$ ($a_{p+k}{\geq}0$, $p{\in}N=\left{1,2,{\cdots}\right}$) which are analytic and p-valent in the unit disc $U=\left{z:{\mid}z:{\mid}<1\right}$ and satisfying $Re\left{\frac{D^{n+p-1}f(\approx))^{\prime}}{pz^{p-a}\right}>{\alpha},0{\leq}{\alpha}<1,n>-p,z{\in}U.$ In this paper we obtain sharp results concerning coefficient estimates, distortion theorem, closure theorems and radii of p-valent close-to- convexity, starlikeness and convexity for the class $Q_{n+p-1}$ ($\alpha$). We also obtain class preserving integral operators of the form $F(z)=\frac{c+p}{z^{c}}\int_{o}^{z}t^{c-1}f(t)dt.$ c>-p $F\left(z\right)=\frac{c+p}{z^{c}}\int_{0}^{z} t^{c-1}f\left(t \right)dt. \qquad c>-p$ for the class $Q_{n+p-1}$ ($\alpha$). Conversely when $F(z){\in}Q_{n+p-1}(\alpha)$, radius of p-valence of f(z) has been determined.

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ON A CLASS OF MULTIVALENT FUNCTIONS WITH NEGATIVE COEFFICIENTS

  • Shukla, S.L.;Chaudhary, A.M.;Owa, S.
    • Kyungpook Mathematical Journal
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    • 제28권2호
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    • pp.129-139
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    • 1988
  • Let $T^{\alpha}_{\lambda}$(p, A, B) denote the class of functions $$f(z)=z^p-{\sum\limits^{\infty}_{k=1}}{\mid}a_{p+k}{\mid}z^{p+k}$$ which are regular and p valent in the unit disc U = {z: |z| <1} and satisfying the condition $\left|{\frac{{e^{ia}}\{{\frac{f^{\prime}(z)}{z^{p-1}}-p}\}}{(A-B){\lambda}p{\cos}{\alpha}-Be^{i{\alpha}}\{\frac{f^{\prime}(z)}{z^{p-1}}-p\}}}\right|$<1, $z{\in}U$, where 0<${\lambda}{\leq}1$, $-\frac{\pi}{2}$<${\alpha}$<$\frac{\pi}{2}$, $-1{\leq}A$<$B{\leq}1$, 0<$B{\leq}1$ and $p{\in}N=\{1,2,3,{\cdots}\}$. In this paper, we obtain sharp results concerning coefficient estimates, distortion theorem and radius of convexity for the class $T^{\alpha}_{\lambda}$(p, A, B). It is further shown that the class $T^{\alpha}_{\lambda}$(p, A, B) is closed under "arithmetic mean" and "convex linear combinations". We also obtain class preserving integral operators of the form $F(z)=\frac{p+c}{z^c}{\int^z_0t^{c-1}}f(t)dt$, c>-p, for the class $T^{\alpha}_{\lambda}$(p, A, B). Conversely when $F(z){\in}T^{\alpha}_{\lambda}$(p, A, B), radius of p valence of f(z) has also determined.

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The effect of reinforcing methods on fracture strength of composite inlay bridge (강화재의 사용 방법이 복합 레진 인레이 브릿지의 파괴 강도에 미치는 영향)

  • Byun, Chang-Won;Park, Sang-Hyuk;Sang-Jin, Park;Choi, Kyoung-Kyu
    • Restorative Dentistry and Endodontics
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    • 제32권2호
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    • pp.111-120
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    • 2007
  • The purpose of this study is to evaluate the effects of surface treatment and composition of reinforcement material on fracture strength of fiber reinforced composite inlay bridges. The materials used for this study were I-beam, U-beam TESCERA ATL system and ONE STEP(Bisco, IL, USA). Two kinds of surface treatments were used; the silane and the sandblast. The specimens were divided into 11 groups through the composition of reinforcing materials and the surface treatments. On the dentiform, supposing the missing of Maxillary second pre-molar and indirect composite inlay bridge cavities on adjacent first pre-molar disto-occlusal cavity, first molar mesio-occlusal cavity was prepared with conventional high-speed inlay bur. The reinforcing materials were placed on the proximal box space and build up the composite inlay bridge consequently. After the curing, specimen was set on the testing die with ZPC. Flexural force was applied with universal testing machine (EZ-tester; Shimadzu, Japan). at a cross-head speed of 1 mm/min until initial crack occurred. The data was analyzed using one-way ANOVA/Scheffes post-hoc test at 95% significance level. Groups using I-beam showed the highest fracture strengths (p<0.05) and there were no significant differences between each surface treatment (p>0.05) Most of the specimens in groups that used reinforcing material showed delamination. 1. The use of I-beam represented highest fracture strengths (p<0.05) 2. In groups only using silane as a surface treatment showed highest fracture strength, but there were no significant differences between other surface treatments (p>0.05). 3. The reinforcing materials affect the fracture strength and pattern of composites inlay bridge. 4 The holes at the U-beam did not increase the fracture strength of composites inlay bridge.

Partial Characterization and Induction of Ferulic Acid Esterase and Xylanase from Pseudomonas sp. LG2 (Pseudomonas sp. LG2의 Ferulic acid esterase 및 Xylanase 유도와 부분적 특성)

  • Kim, Yong-Gyun;Lee, Sang-Mong;Park, Hyun-Chul;Kim, Keun-Ki;Son, Hong-Joo
    • Journal of Life Science
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    • 제17권4호
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    • pp.568-574
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    • 2007
  • Lignin degrading bacterium Pseudomonas sp. LG2 was able to degrade lignin substrate to a lot of APPL compound. APPL compound was detected in culture supernatants from Pseudomonas sp. LG2 grown with BSC(brewer's spent grain). FAE(ferulic acid esterase) and xylanase are induced from Pseudomonas sp. LG2 in the presence of carbon sources such as oat spelt xylan, HBSG I, II(hydrolyzed brewer's spent grain I, II) and AFBSG(autoclaved fraction from brewer's spent grain). However, xylanase and FAE are not induced by growth of Pseudomonas sp. LG2 on xylose and arabinose. Pseudomonas sp. LG2 is grown on medium containing oat spelt xylan, HBSG I, II and AFBSG and the induction of FAE and xylanase activities of extracellular proteins determined during 14 days. Maximum level of xylanase activity(5.3 U/mg) found at 6 days in culture contained oat spelt xylan as carbon source, whereas maximum level of FAE activity(15.4 mU/mg) was found at 8 days in culture contained AFBSG as carbon source. Most ferulic acid was released in culture supernatants when Pseudomonas sp. LG2 grown on oat spelt xylan, HBSG I, II and AFBSG. FAE of extracellular enzymes was also specific activity on methyl ferulic acid, methyl caffeic acid and methyl p-coumaric acid respectively, but not methyl sinapinic acid, methyl vanillic acid and methyl gallic acid.

CYCLES IN T-GRAPHS

  • SREENIVASULU REDDY L;MAHESH KUMAR T;KUMARASWAMY ACHARI B
    • Journal of applied mathematics & informatics
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    • 제42권5호
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    • pp.1137-1144
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    • 2024
  • We are concerned with the T-Graphs, denoted by GT, which are graphs defined based on the Topological structure of the given set. Precisely, for a given topology T on a set X, a T-Graph G = (V,E) is an undirected graph. In this graph, the vertex set V is P(X) and the edge set E consists of all unordered pairs of nodes u and v in V, denoted by (u, v). The pair of nodes satisfies the condition that u ∈ T and uc ∩ v ∈ T, where uc is the complement of the subset u of X. The primary objective of this paper is threefold: (i) Find that the number of triangles (ii) we intend to establish that the number of rectangles and (iii) the number of Hamiltonian cycles and Eulerian closed trials in a graph GT.

A Highly Active Alpha Amylase from Bacillus licheniformis: Directed Evolution, Enzyme Characterization and Structural Analysis

  • Liu, Yihan;Fan, Shuai;Liu, Xiaoguang;Zhang, Zhimeng;Wang, Jianling;Wang, Zhengxiang;Lu, Fuping
    • Journal of Microbiology and Biotechnology
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    • 제24권7호
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    • pp.898-904
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    • 2014
  • The stability of Bacillus licheniformis alpha-amylase (BLA) under acid condition was enhanced through direct evolution using the error-prone polymerase chain reaction. One beneficial mutation site, H281I, was obtained in BLA. The specific activity of H281I was 161/352 U/mg, which was 62.6/27.5% higher than that of the wild-type (WT) (99/276 U/mg) at pH 4.5/6.5 and $95^{\circ}C$. The pH optimum for H281I was decreased about 1 unit, whereas no significant changes of optimum temperature and thermostability were observed compared with the wild type (WT). The $k_{cat}/K_m$ value of H281I was 1.7-/1.4-fold higher at pH 4.5/6.5, respectively, than that of WT. The structure model analysis indicated that the H281I mutation altered the predicted interaction between the amino acid residues at 281 and 273, thus creating a conducive local environment for substrate binding, as reflected by its decreased $K_m$, and consequently increased the specific activity.

Extreme spirallike products

  • Lee, Suk-Young;David Oates
    • Communications of the Korean Mathematical Society
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    • 제10권4호
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    • pp.875-880
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    • 1995
  • Let $S_p(\alpha)$ denote the class of the Spirallike functions of order $\alpha, 0 < $\mid$\alpha$\mid$ < \frac{\pi}{2}$ Let $\Pi_N$ denote the subset of $S_p(\alpha)$ consisting of all products $z\Pi^N_{j=1}(1-u_j z)^{-mt_j}$ where $m = 1 + e^{-2i\alpha},$\mid$u_j$\mid$ = 1, t_j > 0$ for $j = 1, \cdots, N$ and $\sum^{N}_{j=1}{t_j = 1}$. In this paper we prove that extreme points of $S_p(\alpha)$ may be found which lie in $\Pi_N$ for some $N \geq 2$. We are let to conjecture that all exreme points of $S_p(\alpha)$ lie in $\Pi_N$ for somer $N \geq 1$ and that every such function is an extreme point.

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Characterization of NAD(P)H-nitroreductase Purified from the TNT-degrading Bacterium, Stenotrophomonas sp. OK-5 (폭약 TNT 분해세균 Stenotrophomonas sp. OK-5에서 분리된 NAD(P)H-nitroreductase의 정제 및 특성 연구)

  • Ho, Eun-Mi;Cheon, Jae-U;Gang, Hyeong-Il;O, Gye-Heon
    • Korean Journal of Microbiology
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    • 제39권4호
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    • pp.223-229
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    • 2003
  • The purpose of this work was to perform the characterization of NAD(P)H-nitroreductase isolated from Stenotrophomonas sp. OK-5 capable of degrading 2,4,6-trinitrotoluene (TNT). Initially, NADP(H)-nitroreductase by a series of purification processes including ammonium sulfate precipitation, DEAE-sepharose, andQ-sepharose was prepared. From samples harvested from fraction collector, three different fractions (I, II & III)having the enzyme activity of NAD(P)H-itroreductase were detected. Specific activities of three fractions I, II,and III of NAD(P)H-nitroreductase were determined to approximately 5.06 unit/mg, 4.95 unit/mg and 4.86 unit/mg, and concentrated to 10.5, 9.8, and 8.9-fold compared to crude extract, respectively. Among these three fractions,the fraction I of NAD(P)H-nitroreductase demonstrated the highest specific activity in this experiment. Several factors affecting on the enzyme activity of NAD(P)H-nitroreductase (fractions I, II & III) were investigated.The optimum temperature of all NAD(P)H-nitroreductase (fractions I, II & III) was 30oC, and the optimal pH was approximately 7.5. Metal ions such as Ag+, Cu2+, Hg2+ inhibited approximately 80% enzyme activity of all NAD(P)H-nitroreductase, and the enzyme activities were decreased about 30-40% inhibition in the presence of Mn2+ or Ca2+. However, Fe3+ showed stimulatory effect on the enzyme activity. The molecular weights of NAD(P)H-nitroreductase (fractions I, II & III) were measured about 27 kDa on the SDS-PAGE.

제올라이트에 의한 농약의 흡착

  • 감상규;김길성;안병준;이민규
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 한국지하수토양환경학회 2001년도 총회 및 춘계학술발표회
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    • pp.7-10
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    • 2001
  • Adsorption of the pesticides (phosphamidon, fenitrothion, triadimefon and diniconazole) in natural zeolite (CL $I_{N}$) and several synthetic zeolites was incestigated. The pesticides were not adsorbed on zeolites (Na-Pl, SOD, ANA, JBW and CAN) synthesized from Jeju scoria. The distribution coefficient ( $K_{D}$) and the Freundlich constant ( $K_{F}$) decreased in the following sequences. FC $C_{W}$ (waste catalytic cracking catalyst)>FA $U_{F}$ (FAU Synthesized from coal fly ash)>(FAU+Na-Pl)$_{SF}$ (the mixture of FAU and Na-Pl synthesized from the ratio of Jeju scoria 6 to coal fly ash 4 by weight)>CL $I_{N}$ among the zeolites; diniconazole>fenitrothion> triadimefon>phosphamidon. As the temperature was increased, the amount of pesticide adsorbed per unit mass of zeolite increased for FC $C_{W}$, FA $U_{F}$ and (FAU+Na-Pl)$_{SF}$ but it decreased for CL $I_{N}$, for all the pesticides used in this study. It was independent of pH for phosphamidon, fenitrothion and triadimefon, but decresed as pH was increased for all the zeolites used in this study.y.udy.y.y.y.y.y.y.

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