• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.317-323
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• 2006

The objective of this study is to test and modify the gantry travel mechanism of an ALV(automated lifting vehicle) as a main technology of development of ALV. For the purpose of the improvement of container terminal productivity, this study performed to develop the ALV as a part of R&D. In order to verify the design data and detect the errors of detailed drawings, we performed the various limited weight test under load and unload conditions. Through the reflect of final drawing based on the this test, we could use to produce the more complete vehicle.

• Journal of Microbiology
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• v.41 no.4
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• pp.295-299
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• 2003

Adenovirus (Ad) precursor to the terminal protein (pTP) plays an essential roles in the viral DNA replication. Ad pTP serves as a primer for the synthesis of a new DNA strand during the initiation step of replication. In addition, Ad pTP forms organized spherical replication foci on the nuclear matrix (NM) and anchors the viral genome to the NM. Here we identified the interferon inducible gene product 1-8D (Inid) as a pTP binding protein by using a two-hybrid screen of a HeLa cDNA library. Of the clones obtained in this assay, nine were identical to the Inid, a 13-kDa polypeptide that shares homology with genes 1-8U and Leu-13/9-27, most of which have little known functions. The entire open reading frame (ORF) of Inid was cloned into the tetracycline inducible expression vector in order to determine the biological functions related with adenoviral infection. When Inid was introduced to the cells along with adenoviruses, fifty to sixty percent of Ad-infected cells expressing Inid had rounded morphology, which was suggestive of apoptosis. Results from the terminal deoxynucleotidyl transferase (TdT) and DNA fragmentation assays confirmed that Inid induces apoptosis in Ad-infected or in uninfected cells. The Inid binding to pTP may target the cell for apoptotic destruction as a host defense mechanism against the viral infection.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.160-170
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• 2016

The increasing spread of antibiotic-resistant pathogens has raised the interest in alternative antimicrobial treatments. In our study, the functionally active gram-negative bacterium bacteriophage CP933 endolysin was produced in Nicotiana benthamiana plants by a combination of transient expression and vacuole targeting strategies, and its antimicrobial activity was investigated. Expression of the cp933 gene in E. coli led to growth inhibition and lysis of the host cells or production of trace amounts of CP933. Cytoplasmic expression of the cp933 gene in plants using Potato virus X-based transient expression vectors (pP2C2S and pGR107) resulted in death of the apical portion of experimental plants. To protect plants against the toxic effects of the CP933 protein, the cp933 coding region was fused at its Nterminus to an N-terminal signal peptide from the potato proteinase inhibitor I to direct CP933 to the delta-type vacuoles. Plants producing the CP933 fusion protein did not exhibit the severe toxic effects seen with the unfused protein and the level of expression was 0.16 mg/g of plant tissue. Antimicrobial assays revealed that, in contrast to gram-negative bacterium E. coli (BL21(DE3)), the gram-positive plant pathogenic bacterium Clavibacter michiganensis was more susceptible to the plant-produced CP933, showing 18% growth inhibition. The results of our experiments demonstrate that the combination of transient expression and protein targeting to the delta vacuoles is a promising approach to produce functionally active proteins that exhibit toxicity when expressed in plant cells.

• Journal of Institute of Control, Robotics and Systems
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• v.15 no.11
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• pp.1150-1156
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• 2009

The spread of wireless Internet technology development and applications with location information in the form of location-based services are more varied. In particular, where you recognize the location of objects such as people and things, and to provide valuable services based on ubiquitous, location-based services (Ubiquitous Location Based Services: u-LBS) is emerging as an important service. In this paper precise location data to the car crash through the location and offers related service system. In this paper the precise location tracking proposed by the concept of the Rail, road, to extract the location Data Matching Data and the current location is obtained. System used in GPS Packet and information about the location of the vehicle collision and the collision time, the vehicle consists of NodeID is about. Using these data, a packet is to be created when the conflict between vehicles in the vehicle will be sent to Gateway. Gateway to the packets that were sent from the Server to determine whether the conflict is that in an emergency situation, Emergency Center for location information and let me know whether or not the conflict will be measured. Also, for those on the outside of an emergency such as a family related to the wireless terminal wireless (PDA, cell phone) is to let me know. Server get into the conflict that was configured to store information on the Database. Additionally, the proposed u-LBS system to verify the validity of the experiment was performed.

• The KIPS Transactions:PartB
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• v.14B no.6
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• pp.401-406
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• 2007

This study discusses the development of a u-Health care system that can detect quickly and cope actively with emergent situations of chronic disease patients who lead everyday life. If a patient's emergent situation is detected by personal health care host(PHCH), which is composed of acceleration and vibration sensors, GPS, and CDMA communication module, a text message on the patient's current location is transmitted to the hospital and the guardian's mobile terminal so that they can cope with the situation immediately. Especially, the system analyzes data from sensors by using neural network and determines emergent situations such as syncope and convulsion promptly. The exact location of patients can also be found in the electronic map by using GPS information. The experiments show that this system is very effective to find emergencies promptly for chronic disease patients who cannot take care of themselves and it is expected to save many lives.

• 한국ITS학회:학술대회논문집
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• 2008.11a
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• pp.570-574
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• 2008

The 'gate non-stop system utilizing RFID (900MHz)', which is the core of this research, is the system which connects RFID to recognize the vehicle information without stopping when a vehicle (trailer) passes a gate to carry in or out containers in a terminal and automatically provides the container information provided by the terminal for the RFLDU device installed in the vehicle.In order to design and implement this, the RFID technical section uses a UHF band (900MHz) RFID tag and a reader and implements a RFID middleware and an application program for smooth data collection and execution (operation). In addition, the system stability was verified through experiments and operations of the system implemented in this research at real harbors/quays, and based on the verified result, the maximization of vehicle (trailer) and terminal productivity and the reduction of distribution cost are expected.

• Animal cells and systems
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• v.1 no.2
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• pp.323-328
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• 1997

We have previously shown that chick muscle extracts contained at least 10 different ubiquitin C-terminal hydrolases (UCHs). In the present studies, one of the enzymes, called UCH-9, was purified by conventional chromatographic procedures using $^{125}l$-labeled ubiquitin-${\alpha}$NH-MHISPPEPESEEEEE HYC (Ub-PESTc) as a substrate. The purified enzyme behaved as a 27-kDa protein under both denaturing and nondenaturing conditions, suggesting that it consists of a single polypeptide chain. It was maximally active at pHs between 7 and 8.5, but showed little or no activity at pH below 6 and above 10. Lice other UCHs, its activity was strongly inhibited by sulfhydryl blocking reagents, such as iodoacetamide, and by Ub-aldehyde. In addition to Ub-PESTc, UCH-9 hydrolyzed Ub-aNH-protein extensions, including Ub-${\alpha}NH$-carboxyl extension protein of 80 amino acids and Ubo-${\alpha}NH$-dihydrofolate reductase. However, this enzyme was not capable of generating free Ub from mono-Ub-${\varepsilon}NH$-protein conjugates and from branched poly-Ub chains that are ligated to proteins through ${\varepsilon}NH$-isopeptide bonds. This enzyme neither could hydrolyze poly-His-tagged di-Ub. These results suggest that UCH-9 may play an important role in production of free Ub and ribosomal proteins from their conjugates.

• Journal of Applied Biological Chemistry
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• v.44 no.3
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• pp.113-118
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• 2001

Xylose (glucose) isomerase was purified to homogeneity from cell-extracts of Streptomyces chibaensis J-59 via ammonium sulfate precipitation followed by chromatography on DEAE-cellulose, and gel filtration on Sephacryl S-300. The purified enzyme is a homotetramer with a native molecular mass of 180 kDa and a subunit molecular mass of 44 kDa. The amino acid N-terminal sequence of glucose isomerase from S. chibaensis J-59 was determined to be Ser-Tyr-Gln-Pro-Thr-Pro-Glu-Asp-Arg-Phe-Thr-Phe-Gly-Leu. The first 14 amino acids of the N-terminal sequence of the enzyme showed strong analogies with N-terminal sequences of glucose isomerase produced by other Streptomyces spp. The optimum pH and temperature for activity were 7.5 and 85, respectively. The purified enzyme required $Mg^{2+}$, $Co^{2+}$, and $Mn^{2+}$ for the activity, $Mg^{2+}$ being the most effective. The enzyme was not inhibited by $Ca^{2+}$, but was inhibited by $Hg^{2+}$, $Ag^+$, and $Cu^{2+}$. The $K_m$, $V_{max}$, and $k_{cat}$ values of S. chibaensis J-59 isomerase for glucose were 83 mM, 40.9 U/mg, and $1,843min^{-1}$, respectively. In the presence of $Co^{2+}$, cell-free enzymes retained 100% without loss of activities by the heat-treatment at $70^{\circ}C$ for 7 days. The enzyme retained 50% residual activity after heating at $85^{\circ}C$ for 13.5 h, at $90^{\circ}C$ for 126 min. The enzyme is more thermostable than any other glucose isomerases of Streptomyces spp.

• Journal of the Korean Geotechnical Society
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• v.23 no.7
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• pp.87-98
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• 2007

A series of dilatometer test, field vane test, and $CK_0U$ triaxial test were performed for clayey soils of Busan new port site to develop the relationships between undrained shear strength and the DMT results. Normalized undrained shear strength is turned out to be $S_{u(CKU)}/{\sigma}'_v=0.30{\sim}0.35\;for\;CK_0U$ triaxial test and ${\mu}S_{u(VST)}/{\sigma}'_v=0.20{\sim}0.22$ for vane shear test. By comparing the undrained shear strength estimated from DMT indices with the results measured by in-situ vane test or $CK_0U$ triaxial test, two methods to predict the undrained shear strength from DMT results are suggested. One is based on the relationship between $S_u/{\sigma}'_v$ and horizontal stress index (KD) while another method comes from $N_c-I_D$ and $N_c-E_D$ correlation. It was observed that the method based on $N_c-I_D\;or\;N_c-E_D$ relation shows slightly better accuracy than the one based on $K_D$ although all of the methods suggested in this study provided comparable values of predicted undrained shear strength. Since the definitions of $I_D\;and\;E_D$ contain $p_1-p_0$, in which soil condition is reflected, it is believed that the prediction method using $N_c$ is capable of taking a material type into consideration.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.4
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• pp.472-482
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• 2007

This study deals with the design and implementation results for a high-capacity vol-oxidizer that can convert Uranium Dioxide pellets to $U_3O_8$ powder for up to several tens of kg HM/batch. We developed two versions of the $1^{st}$ vol-oxidizer and the $2^{nd}$ vol-oxidizer. Through an experiment with the $1^{st}$ vol-oxidizer, we deduced some problems concerning the design considerations such as the recovery rate of $U_3O_8$, the oxidation time of the Uranium Dioxide pellets, the exothermic reaction, and the powder dispersion. From the analyses of the drawbacks of the $1^{st}$ vol-oxidizer, we devised some novel items such as a folding type mesh, vibrators, and mixing blades. Also, we used the Stokes and Density ratio Eq. to determine the most reasonable flux for preventing a powder dispersion. Compared with the results of the $1^{st}$ vol-oxidizer, we showed that both the permeability of the $U_3O_8$ powders and the oxidation rate of the Uranium Dioxide pellets of the $2^{nd}$ vol-oxidizer were remarkably increased, and the temperature of the reactor was controlled well in spite of an exothermic reaction. Also, the powder was not entirely dispersed through the outlet of the voloxidizer. The experimental results of this work can help in the design of a novel and efficient vol-oxidizer with a higher capacity.