• Journal of the Institute of Electronics Engineers of Korea SD
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• v.38 no.10
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• pp.754-764
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• 2001

Class A bipolar second-generation current conveyor (CCII) with low current-input impedance and its offset-compensated CCII for high-accuracy current-mode signal processing are proposed. The CCIIs consist of a regulated current-cell for current input, a emitter follower for voltage input, and a cascode current mirror lot current output. In these architecture, the two input stages are coupled by current mirror to reduce the current input impedance. Experiments show that the CCII has impedance of 8.4 Ω and offset voltage of 40 mV at current input terminal. To reduce this offset, the offset-compensated CCII adopts diode-connected npn and pnp transistor in the proposed CCII. Experiments show that the offset-compensated CCII has current input impedance of 2.1 Ω and offset voltage of 0.05 mV. The 3-dB cutoff frequency of the CCIIs when used as a voltage follower extends beyond 30 MHz. The power dissipation is 7.0 mW

• Journal of Korean Traditional Oncology
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• v.13 no.1
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• pp.1-11
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• 2008

Objectives: To grasp the traditional stream of cancer treatment inherited from the previous doctors. Methods: The author's research has been performed cancer treatment based on JIJU(積聚) taking the original text as a reference. Results & Conclusion: The general three outlines and five detailed rules of cancer treatment have been obtained as follows. The first outline of cancer treatment is that reinforced vital function makes cancer reduce naturally. the second is that Harmless cancer can coexist in human beings and aging with them. and the third is that Elimination and reinforcing therapies should be executed in appropriate era and those therapies should utilize appropriate methods. The first detailed rule of cancer treatment is when using reinforcing therapy, it must applicate mildly and when using elimination therapy, it must applicate calmly. The second detailed rule is that the methods of cancer treatment are different from each cancer stage. The concentration should be made on reinforcing therapy at early stage while reinforcing and elimination therapies must be conducted together at middle stage. At terminal stage reinforcing therapy is the sole method to be taken. The third detailed rule is that the basis property of cancer drug is warm nature and extremely biased property should be avoided and when complication arises (eg. inflamatory disease, cancer fever, etc), cold or cool nature can be applied. The fourth detailed rule is that Cancer drug must have the effect eliminating the blood stasis, phlegm and excessive fluid, all together. The fifth detailed rule is that Physicians have to control patient's stress or stress related symptom and teach patients about right way of taking care of themselves and patients should take hygienic rules with their free will by themselves (eg diet, exercise, stress, etc)

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.183-188
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• 2008

A fibrinolytic serine protease was purified from the fruiting bodies of an edible mushroom, Albatrellus confluens. The enzyme had a molecular mass of 30086.41 Da, as measured by MALDI-TOF mass spectrometry. The N-terminal amino acid sequence of the enzyme was Glu-Thr-Val-Thr-Glu-Thr-Thr-Ala -Pro-Trp-Gly-Leu-Ser-Arg-Ile. It displayed optimal activity at $50^{\circ}C$ and within a pH range of $8.0{\sim}10.0$, suggesting that the enzyme is an alkaline protease. The enzyme was stable up to $30^{\circ}C$. The enzyme displayed a strong substrate specificity for the synthetic peptide, N-Suc-Ala-Ala-Pro-Phe pNA. The enzyme activity was completely inhibited by addition of PMSF, indicating that the enzyme is a serine protease. No inhibition was observed following addition of E-64, pepstatin, or EDTA. The activity of the purified enzyme was decreased in the presence $Fe^{2+}$ or $Co^{2+}$, and the enzyme was completely inhibited by addition of $Hg^{2+}$. From these results, we propose that Albatrellus confluens could be used for biofunctional foods development and has potential therapeutic value for the treatment of vascular diseases.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.160-166
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• 2008

A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoding 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with $endo-{\beta}-1,4-D-mannanase$ from Bacillus circulans strain K-1 of the glycoside hydrolase family 5 (GH5). The sequence encoding the mature protein was cloned into the pET-22b(+) vector and expressed in Escherichia coli as a recombinant fusion protein containing an N-terminal hexahistidine sequence. The fusion protein was purified by $Ni^{2+}$ affinity chromatography and its hexahistidine tag cleaved to yield a 31-kDa ${\beta}$-mannanase having a specific activity of 481.55U/mg. The optimal activity of the purified protein, MANB48, was at $58^{\circ}C$ and pH 7.6. The hydrolysis product on substrate locust bean gum included a monosaccharide and mainly oligosaccharides. The recombinant MANB48 may be of potential use in the feed industry.

• Korean Journal of Pharmacognosy
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• v.36 no.2 s.141
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• pp.88-92
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• 2005

Apoptosis plays an important role in autoimmune chronic (Hashimoto's) thyroiditis, a disorder that often results in hypothyroidism. The goal of this study was to induce apoptosis by the combination of inflammatory cytokines, interferon $(IFN)-{\gamma}$ and tumor necrosis factor $(TNF)-{\alpha}$, and to investigate a potential role of medicinal plants in the thyroid follicular cells (FRTL) in vitro. The apoptosis was evaluated by cellular viability, DNA fragmentation, and terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) assay. Extract of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix, and Cnidii Rhizoma) $(0.3{\sim}9.0mg/ml)$ was shown to maintain the viability of cells treated with $IFN-{\gamma}(100U/ml)$ and $TNF-{\alpha}$ (0.5 ng/ml). FRTL cells were found to undergo DNA fragmentation with the inflammatory cytokines. The extract of GGT inhibited DNA fragmentation in dose-dependent manner. The cells with TUNEL-positive nuclei were detected with $IFN-{\gamma}$ and $TNF-{\alpha}$ treatment. The number of TUNEL-positive cells decreased with the treatment of extract of GGT. These results indicate that medicinal plants inhibit the occurrence of apoptosis in thyroid follicular cells, therefore, may have therapeutic potential in the treatment of autoimmune chronic thyroiditis.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.12A
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• pp.1852-1861
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• 1999

In this paper, CDMA Reservation ALOHA scheme which can reduce multiple access interference and packet collision is proposed to support multi-traffic such as voice and random data with and without priority. In this scheme, the time slot is divided into two stage, access and transmission stage. Only packets with spreading codes assigned from base station in access stage can transmit their packets in transmission stage, so MAI can be reduced. To reduce packer collision in access stage, the code reservation and access permission probability are used. Code reservation is allowed for voice traffic and continuous traffic with priority using piggyback and access permission probability based on the estimation of the number of contending users in the steady-state is adaptively applied to each traffic terminal. Also, we analyzed and simulated the numerical performances required for each traffic using Markov chain modeling in a single cell environment.

• Journal of KIISE:Computing Practices and Letters
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• v.5 no.4
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• pp.498-506
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• 1999

본 논문에서는 ATM 통신프로토콜을 위한 자동시험시스템인 ACTS(ATM Conformance Test System)의 개발과 적용을 소개한다. ACTS는 ITU-T 또는 ATM Forum의 ATM 사용자-망 접속표준을 준용하는 ATM 단말과 망장비의 프로토콜적합성을 확인하는데 사용된다. 본 논문에서는 ACTS 개발에 사용된 방법을 소개한 뒤, ACTS를 실제 ATM 단말과 장비에 적용한 사례들을 소개한다. ACTS의 적용을 통하여 시험대상 장비들이 지닌 프로토콜구현상의 문제점을 파악하였고, 문제에 대한 원인분석을 수행하였다. 또한 이러한 문제점들이 상호운용에 미치는 장애를 예측함으로써, ACTS를 상호운용하는 ATM 장비를 확보하기 위한 유용한 도구로 사용할 수 있음을 보인다.Abstract This paper presents development and application of ACTS(ATM Conformance Test System), an automated test system for ATM protocols. ACTS is a test system that checks conformance of ATM terminal and network equipment implementing either ITU-T or ATM Forum user-network interface. This paper, after presenting the methodology and process used for developing ACTS, conducts case studies of its applications to real ATM equipment. By applying ACTS, we were able to detect numerous problems in protocol implementations of ATM equipment and analyse causes of the problems, thereby demonstrating the efficacy of ACTS as an efficient automated testing tool. Furthermore, by predicting the potential effects of the problems on interoperability, we show how ACTS can be used as a useful tool for ensuring interoperable ATM equipment.

• Journal of Korean Medicine Rehabilitation
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• v.21 no.2
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• pp.15-30
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• 2011

Objectives : The present study investigated the effects of Achyranthis Radix on short-term memory, apoptotic neuronal cell death in the hippocampus following transient global ischemia in gerbils. Methods : The gerbils were divided into 5 groups(n=10); Sham operation group, ischemia-induced group, ischemia-induced and 50 mg/kg Achyranthis Radix-treated group, ischemia-induced and 100 mg/kg Achyranthis Radix-treated group, ischemia-induced and 200 mg/kg Achyranthis Radix-treated group. For this study, a step-down avoidance task, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay, immunohistochemistry for caspase-3 and BrdU(5-Bromo-2'-deoxyuridine), and western blotting for bax, bcl-2 were performed. Results : The results revealed that ischemic injury impaired short-term memory and increased apoototic neuronal cell death in the hippocampal CA1(cornu ammonis area 1) region. Ischemic injury enhanced cell proliferation in the hippocampal CA1 region, the compensatory and adaptive process for excessive apoptosis. Achyranthis Radix treatment improved short-term memory by suppressing ischemia-induced apoptotic neuronal cell death in the hippocampal CA1 region. Also, Achyranthis Radix suppressed the ischemia-induced increase in cell proliferation in the hippocampal CA1 region. Conclusions : We showed that Achyranthis Radix alleviates ischemia-induced apoptotic neuronal cell death, thus facilitates the recovery of short-term memory impairment induced by ischemic cerebral injury.

• Journal of the Korea Society of Computer and Information
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• v.17 no.3
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• pp.85-93
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• 2012

In this paper, I designed the satellite system, using the MF-TDMA (Multi Frequency-Time Division Multiple Access) DAMA (Demand-Assigned Multiple Access) method, that allocates the satellite resources more effectively to prevent a large quantity data discard when Earth Terminals (ETs) request their satellite network resource to Network Controller (NC) by using their history information, such as traffic pattern or traffic class which have been receiving, and the network controller allocates the satellite network resource and send to earth terminals by using his history information that earth terminals have been requested already. The U.S. Military Warfighter Information Network-Tactical (WIN-T) community has selected the L-3 Linkabit MF-TDMA DAMA Network Centric Waveform (NCW) as the networking standard for full-mesh IP over SHF satellite communications. In the MF-TDMA DAMA satellite network, network controller allocates the satellite network resources and enables maximum 255 earth terminals to communicate each other for periodic satellite network resource requests of earth terminals.

• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.256-262
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• 2011

A 20.9-kDa metalloprotease was isolated from dried fruiting bodies of the wild basidiomycete mushroom Lepista nuda. The N-terminal amino acid sequence of the protease was seen to be ATFVLTAATNTLFTA, thus displaying no similarity with the sequences of previously reported metalloproteases. The protease was purified using a procedure that entailed ion-exchange chromatography on CM-Cellulose, Q-Sepharose, and Mono S, and FPLC-gel filtration on Superdex 75. The protease functioned at an optimum pH of 7.0 and an optimum temperature of $50^{\circ}C$. It was also noted that the protease demonstrated a proteolytic activity of 1,756 U/mg toward casein. The $K_m$ of the purified protease toward casein was 6.36 mg/ml at a pH of 7.0 and with a temperature of $37^{\circ}C$, whereas the $V_{max}$ was 9.11 ${\mu}g\;ml^{-1}\;min^{-1}$. The activity of the protease was adversely affected by EDTA-2Na, suggesting that it is a metalloprotease. PMSF, EGTA, aprotinin, and leupeptin exerted no striking inhibitory effect. The activity of the protease was enhanced by $Fe^{2+}$, but was curtailed by $Cd^{2+}$, $Cu^{2+}$, $Hg^{2+}$, $Pb^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ ions. The protease also exhibited inhibitory activity against HIV-1 reverse transcriptase with an $IC_{50}$ value of 4.00 ${\mu}M$. The $IC_{50}$ values toward hepatoma Hep G2 and leukemia L1210 cells in vitro were 4.99 ${\mu}M$ and 3.67 ${\mu}M$, respectively.