Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Medicinal Plants on Cytokine-induced Apoptosis in Thyroid Cells

생약혼합물이 사이토카인에 의한 갑상선세포의 Apoptosis에 미치는 영향

  • Nam, Kyung-Soo (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Son, Ok-Lye (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Kim, Mee-Kyung (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Park, In-Kyung (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Kim, Cheorl-Ho (Department of Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University) ;
  • Cho, Hyun-Gug (Department of Visual Optics, Kyungwoon University) ;
  • Jeon, Byung-Hun (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Shon, Yun-Hee (Intractable Disease Research Center and Department of Pharmacology, College of Medicine, Dongguk University)
  • 남경수 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실) ;
  • 손옥례 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실) ;
  • 김미경 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실) ;
  • 박인경 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실) ;
  • 김철호 (동국대학교 한의과대학 생화학분자생물학교실) ;
  • 조현국 (경운대학교 안경과학과) ;
  • 전병훈 (원광대학교 한의학대학 병리학교실) ;
  • 손윤희 (동국대학교 난치병한양방치료연구센터 및 의과대학 약리학교실)
  • Published : 2005.06.30
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Abstract

Apoptosis plays an important role in autoimmune chronic (Hashimoto's) thyroiditis, a disorder that often results in hypothyroidism. The goal of this study was to induce apoptosis by the combination of inflammatory cytokines, interferon $(IFN)-{\gamma}$ and tumor necrosis factor $(TNF)-{\alpha}$, and to investigate a potential role of medicinal plants in the thyroid follicular cells (FRTL) in vitro. The apoptosis was evaluated by cellular viability, DNA fragmentation, and terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) assay. Extract of Gamgung-tang (GGT, Glycyrrhizae Radix, black beans, Angelicae Radix, and Cnidii Rhizoma) $(0.3{\sim}9.0mg/ml)$ was shown to maintain the viability of cells treated with $IFN-{\gamma}(100U/ml)$ and $TNF-{\alpha}$ (0.5 ng/ml). FRTL cells were found to undergo DNA fragmentation with the inflammatory cytokines. The extract of GGT inhibited DNA fragmentation in dose-dependent manner. The cells with TUNEL-positive nuclei were detected with $IFN-{\gamma}$ and $TNF-{\alpha}$ treatment. The number of TUNEL-positive cells decreased with the treatment of extract of GGT. These results indicate that medicinal plants inhibit the occurrence of apoptosis in thyroid follicular cells, therefore, may have therapeutic potential in the treatment of autoimmune chronic thyroiditis.

Keywords

References

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