• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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• pp.183-186
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• 2008

유비쿼터스 환경에서 GIS 시스템은 모바일 단말기를 통해 지리정보를 수정, 활용하는 것을 특징으로 하고 있다. 모바일 단말기에 저장된 지리정보는 서버와의 동기화를 통해 최신성을 유지할 수 있어야 한다. 그러나 모바일 환경에서는 제한적인 대역폭으로 인하여 동기화서비스에 한계가 존재한다. 모바일 환경을 고려하여 개발된 ActMAP 시스템에서는 좁은 대역폭 문제를 해결하기 위해서 동기화를 위한 부분 갱신 프로토콜을 제안하고 있으나, 서버에서 모바일 단말기로의 단방향 동기 화만을 지원하기 때문에, 모바일 단말기에서 수집된 지리정보를 서버와 동기화 할 수 없는 문제가 있다. 이 논문에서는 모바일 단말기와 서버간의 양방향 동기화 프로토콜을 제안함으로서 모바일 단말기를 이용한 지리정보의 수집 및 활용을 지원하고자 한다.

• 대한전자공학회논문지SD
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• 제38권11호
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• pp.828-835
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• 2001

고정도 전류-모드 신호 처리를 위한 새로운 바이폴라 트랜스레지스턴스 증폭기(TRA)와 이것의 오프셋 보상된 TRA를 제안하였다. 두 TRA는 전류 입력을 위한 두 개의 전류 폴로워, 전류차를 얻기 위한 전류 가산기, 전류를 전압으로 변환시키기 위한 저항, 그리고 전압 출력을 위한 전압 폴로워로 구성되었다. 오프셋 보상된 TRA는 TRA의 오프셋 전압을 감소시키기 위한 다이오드 결선된 npn과 pnp 트랜지스터를 채용하였다. 시뮬레이션 결과, TRA근 입-출력 단자에서 0.5 Ω의 임피던스와 40 mV의 오프셋 전압을 갖고 있다는 것이 확인되었다. 오프셋 보상된 TRA는 1.1 mV의 오프셋 전압과 0.25 Ω의 임피던스를 갖고 있다. 두 개의 TRA를 단위-이득의 트랜스레지스턴스를 갖는 전류-전압 변환기로 이용할 때 3-dB 차단 주파수는 40 MHz이다. 제안한 두 TRA의 전력 소비는 11.25 mW이다.

• KSBB Journal
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• 제19권5호
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• pp.363-367
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• 2004

Leuconostoc mesenteroides NRRL B-1149 produces various glucoseyltransferases for the synthesis of dextran, levan and glucose-1-phosphate using sucrose as a substrate. A sucrose phosphorylase (1149SPase) was purified from L. mesenteroides NRRL B-1149 culture by using hollow fiber filtration (30 kDa cut off), Toyopearl DEAE 650 M column chromatography and following two times of DEAE-Sepharose column chromatographies. The specific activity of the purified 1149SPase was 25.7 (U/mg) with $16\%$ yield. The 1149SPase showed a molecular size of 56 kDa on denatured $10\%$ SDS-PAGE. The N-terminal amino acid sequence of the enzyme was MEIQNKAM. The optimum pH and temperature of this enzyme were 6.2~6.5 and 37^{circ}C, respectively. It had an apparent K_{m} of 6.0 mM and K_{cat} of 1.62/s for sucrose. 1149SPase crystal was formed by hanging drop diffusion technique using 20 mM calcium chloride dihydrate, 100 mM sodium acetate trihydrate pH 4.6 and $30\%$ 2-methyl-2,4-pentanediol as vaporizing and reservation solution. The 1149SPase catalyzes transferring of glucose from isomaltose or sucrose to salicin and salicyl alcohol by disproportionation reaction or acceptor reaction and synthesized two acceptor products, respectively.

• Biomolecules & Therapeutics
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• 제17권3호
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• pp.276-281
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• 2009

Lactic acid bacteria (LAB) are considered as probiotics with immunostimulatory property. In this study, we investigated the molecular mechanism of its immunostimulating potency on macrophages using combined preparation of LAB (cpLAB). cpLAB is able to strongly stimulate nitric oxide (NO) production as well as inducible NO synthase (iNOS) expression from macrophage-like RAW264.7 cells. The cpLAB-induced NO release seemed to be mediated by extracellular signal-regulated kinase (ERK) but not p38 and C-Jun N-terminal kinase (JNK), since U0126, an ERK inhibitor, clearly suppressed NO production. cpLAB significantly diminished the binding of toll like receptor (TLR)-2 antibody up to 25%, implying that cpLAB-mediated activation of macrophages may be required for the functional activation of TLR-2, but not TLR-4. Therefore, our data suggest that cpLAB may directly allow macrophages to immunostimulating potency via activation of TLR-2 and ERK.

• 한국정보기술응용학회:학술대회논문집
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• 한국정보기술응용학회 2005년도 6th 2005 International Conference on Computers, Communications and System
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• pp.55-60
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• 2005

Ubiquitous computing environment means the computing environment that has taken its position so closely with the ordinary living so much like air or water. In building up the U-Korea, one of the important issues is the social issue from the drastic increase of senior population. The contemporary society has its distinct trend in increase of senior household following the nuclear family orientation, increase of working parents with the advancement of women in society, unable to support seniors for long distance or short distance of business trip and other reasons that the need of senior welfare has been ever more felt. Accordingly, the Ministry of Government Affairs and Home Administration has developed the wireless paging system to make prompt response system for 119 Rescue when the single senior is encountered with emergency situation that is has been widely provided for the socially neglected people such as single senior, the disabled persons and others. Currently, the wireless paging system is operated as the sub-system for emergency rescue information system, but due to the lack of reliability of product, problems of terminal portable transmitter, receptor and others, rejection of beneficiary and lack of knowledge in use, insufficient management and supervision of managing officers, the efficiency has been declined that there is a need of development for the system. Therefore, this study proposes the context aware information structure of the subject of ubiquitous wireless paging system required for the development of the wireless paging system model of ubiquitous environment that improved the problems of currently operated wireless paging system.

• 한국정보통신학회논문지
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• 제14권11호
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• pp.2491-2496
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• 2010

국내 Smart Phone 수요가 급속도로 증가함에 따라 멀티미디어 서비스의 활용도 다양해졌다. Smart Phone 사용자들은 Jail Breaking과 Rooting 등 해킹을 하여 멀티미디어 저작권 콘텐츠를 불법으로 이용하고 있다. 또한 한 미 FTA 체결에 따른 법적 문제제기와 이동통신 단말로서 범죄와 관련성이 높아서, 생성, 저장된 디지털 증거는 증거의 활용도가 높아 모바일 포렌식 연구가 필요하다. 본 논문은 Smart Phone 저작권 위반을 가정한 경우 적법적인 압수 수색의 방법과 주의 할 점을 연구하였다. Smart Phone 저작권 침해 현황과 관련 위반사항들을 방송, 영화, 음악, e-book 등으로 항목별로 조사하였고, 포렌식에 기술을 적용하여 법정에 보고서를 제출하는 방법에 대해 연구하였다. 본 연구 결과는 Smart Phone 범죄 증거 자료 제공과 모바일 포렌식 기술 발전에 기여 할 수 있을 것이다.

• 대한한의학회지
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• 제29권1호
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• pp.95-105
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• 2008

Objectives : Although herbal medicines containing flavonoids have been reported to exert anti-tumor activities, it has not been explored whether Hwang-Jeong (Polygonati sibirici Rhizoma, PsR) exerts anti-tumor activity in human glioma. The present study was therefore undertaken to examine the effect of PsR on cell viability and to determine its underlying mechanism in A172 human glioma cells. Methods : Cell viability was estimated by MTT assay. Reactive oxygen species generation and mitochondrial membrane potential were measured by the fluorescence dyes. The phosphorylation of kinases was evaluated by western blot analysis and caspase activity was estimated using colorimetric assay kit. Results : PsR resulted in loss of cell viability in a dose- and time-dependent manner. PsR did not increase reactive oxygen species (ROS) generation and the PsR-induced cell death was also not affected by antioxidants, suggesting that ROS generation is not involved in loss of cell viability. Western blot analysis showed that PsR treatment caused rapid reduction in phosphorylation of extracellular signal-regulated kinase (ERK) without changes in p38 and Jun-NH2-terminal kinase (JNK). U0126, an inhibitor of ERK, increased the PsR-induced cell death, but inhibitors of p38 and JNK did not affect the cell death. PsR induced depolarization of mitochondrial membrane potential. Caspase activity was not stimulated by PsR and caspase inhibitors did not prevent the PsR-induced cell death. Conclusion : Taken together, these findings suggest that PsR results in human glioma cell death through caspaseindependent mechanisms involving down-regulation of ERK.

• BMB Reports
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• 제38권1호
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• pp.17-23
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• 2005

During the screening of xylanolytic enzymes from locally isolated fungi, one strain BCC14405, exhibited high enzyme activity with thermostability. This fugal strain was identified as Aspergillus cf. niger based on its morphological characteristics and internal transcribed spacer (ITS) sequences. An enzyme with xylanolytic activity from BCC14405 was later purified and characterized. It was found to have a molecular mass of ca. 21 kDa, an optimal pH of 5.0, and an optimal temperature of $55^{\circ}C$. When tested using xylan from birchwood, it showed $K_m$ and $V_{max}$ values of 8.9 mg/ml and 11,100 U/mg, respectively. The enzyme was inhibited by $CuSO_4$, EDTA, and by $FeSO_4$. The homology of the 20-residue N-terminal protein sequence showed that the enzyme was an endo-1,4-$\beta$-xylanase. The full-length gene encoding endo-1,4-$\beta$-xylanase from BCC14405 was obtained by PCR amplification of its cDNA. The gene contained an open reading frame of 678 bp, encoding a 225 amino acid protein, which was identical to the endo-1,4-$\^{a}$-xylanase B previously identified in A. niger.

• The Plant Pathology Journal
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• 제36권5호
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• pp.468-475
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• 2020

Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCV-SX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCV-SX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for "A" and "U" at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1189-1196
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• 2005

The cyclohexanol dehydrogenase (ChnA), produced by Rhodococcus sp. TK6, which is capable of growth on cyclohexanol as the sole carbon source, has been previously purified and characterized. However, the current study cloned the complete gene (chnA) for ChnA and its flanking regions using a combination of a polymerase chain reaction (PCR) based on the N-terminal amino acid sequence of the purified ChnA and plaque hybridization from a phage library of Rhodococcus sp. TK6. A sequence analysis of the 5,965-bp DNA fragment revealed five potential open reading frames (ORFs) designated as partial pte (phosphotriesterase), acs (acyl-CoA synthetase), scd (short chain dehydrogenase), stp (sugar transporter), and chnA (cyclohexanol dehydrogenase), respectively. The deduced amino acid sequence of the chnA gene exhibited a similarity of up to $53\%$ with members of the short-chain dehydrogenase/reductase (SDR) family. The chnA gene was expressed using the pET21 a(+) system in Escherichia coli. The activity of the expressed ChnA was then confirmed (13.6 U/mg of protein) and its properties investigated.