• 동의생리병리학회지
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• 제18권4호
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• pp.1041-1054
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• 2004

Electroacupuncture (EA) has been reported to increase pain threshold, and to enhance the NK cell activity by up-regulation of IFN-γ and endogenous β-endolphin. For the purpose of understanding the molecular mechanism of EA stimulation, we analyzed the gene expression profile of rat hypothalamus, treated on Zusanli (ST36) with EA, in comparison with control group by oligonucleotide chip microarray (Affymetrix GeneChip Rat Neurobiology U34 Array) and real-time RT-PCR. Sprague-Dawley (S-D) male rats were stimulated at the Zusanli (ST36) acupoint in restriction holder. Simultaneously the control group was given only holder stress without EA stimulation. In order to prove the appropriateness of EA treatment, we measured spleen NK cell activity with standard 51Cr release assay. NK cell activity of EA group was significantly increased comparing to control group. The microarray and PCR results show that EA treatment up-regulates expression of genes associated with 1) nerve growth such as NGF induced factor A and VGF, 2) signal transduction such as 5HT3 receptor subunit, AMPA receptor binding protein and Na-dependent neurotransmitter transporter, and 3) anti-oxidation such as superoxide dismutase and glutathione S-transferase. In addition, the activity of the anti-oxidative enzyme, SOD of hypothalamus, liver and RBC was enhanced compared to that of control. The list of differentially expressed genes may implicate further insight on the mechanism of acupuncture effects.

• 한국환경과학회지
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• 제27권4호
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• pp.241-250
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• 2018

The objective of this study was to estimate air quality trends in the study area by surveying monthly and seasonal concentration trends. To do this, the mass concentration of $PM_{10}$ samples and the metals, ions, and total carbon in the $PM_{10}$ were analyzed. The mean concentration of $PM_{10}$ was $33.9{\mu}g/m^3$. The composition of $PM_{10}$ was 39.2% ionic species, 5.1% metallic species, and 26.6% carbonic species (EC and OC). Ionic species, especially sulfate, ammonium, and nitrate, were the most abundant in the $PM_{10}$ and had a high correlation coefficient with $PM_{10}$. Seasonal variation of $PM_{10}$ showed a similar pattern to those of ionic and metallic species. with high concentration during the winter and spring seasons. $PM_{10}$ showed high correlation with the ionic species $NO_3{^-}$ and $NH_4{^+}$. In addition, $NH_4{^+}$ was highly correlated with $SO{_4}^{2-}$ and $NO_3{^-}$. We obtained four factors through factor analysis and determined the pollution sources using the United States Environmental Protection Agency(U.S. EPA) pollution profile. The first factor accounted for 51.1% of $PM_{10}$ from complex sources, that is, soil, motor vehicles, and secondary particles: the second factor indicated marine sources; the third factor, industry-related sources; and the last factor, heating-related sources. However, the pollution profile used in this study may be somewhat different from the actual situation in Korea because it was from US EPA. Therefore, to more accurately estimate the pollutants present, it is necessary to create a pollution profile for Korea.

• 대한감각통합치료학회지
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• 제13권2호
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• pp.21-29
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• 2015

목적 : 뇌성마비아동 중 양하지마비 아동과 일반아동을 대상으로 감각처리 능력을 비교하고자 하였다. 또한 아동을 학령전기와 학령기로 나누어 연령에 따른 감각처리 특성을 알아보고자 하였다. 연구방법 : 경직형 양하지마비 아동과 일반아동의 감각처리 능력을 알아보기 위해 단축형 감각프로파일(Short Sensory Profile: SSP)을 사용하였다. 연구대상은 뇌성마비 중 양하지마비 유형으로 진단받은 만 3~10세 아동과 일반아동을 대상으로 보호자가 직접 설문지 작성을 하도록 하였다. 2013년 11월부터 2014년 2월까지 설문지 배부 및 수거가 이루어 졌으며 경직형 양하지마비 아동 40명, 일반아동 40명의 자료가 분석에 사용되었다. 연구 분석은 집단별 비교를 위해 독립표본 t-test, 연령별 비교를 위해 Mann Whitney U-test를 사용하였다. 연구결과 : 단축형 감각프로파일 총점과 모든 영역에서 경직형 양하지마비 아동이 일반아동보다 점수가 낮게 나타났으며 촉각 민감성과 맛/냄새 민감성을 제외한 5가지 영역과 감각처리 총점에서 두 그룹 간 유의미한 차이가 있는 것으로 나타났다. 학령전기 아동은 총점과 움직임 민감성, 과소반응/특정자극 찾기, 청각 여과하기, 활력이 부족하고 허약함, 시각/청각 민감성에서 일반아동과 유의미한 차이를 보였으며 학령기 아동은 움직임 민감성, 활력이 부족하고 허약함, 시각/청각 민감성에서 유의미한 차이를 보였다. 결론 : 본 연구 결과는 경직형 양하지마비 아동의 감각처리 평가 시 기초자료로 사용될 수 있으리라 생각된다. 앞으로 뇌성마비의 다른 유형을 대상으로 하는 감각처리 능력에 대한 연구가 이루어져야 할 것이다.

• 한국환경과학회지
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• 제28권4호
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• pp.413-422
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• 2019

The objective of this study was to estimate the trends of air quality in the study area by analyzing monthly and seasonal concentration trends obtained from sampled data. To this aim, the mass concentrations of $PM_{2.5}$ in the air were analyzed, as well as those of metals, ions, and total carbon within the $PM_{2.5}$. The mean concentration of $PM_{2.5}$ was $22.7{\mu}g/m^3$. The mass composition of $PM_{2.5}$ was as follows: 31.1% of ionic species, 2.2% of metallic species, and 26.7% of carbonic species (EC and OC). Ionic species, especially sulfate, ammonium, and nitrate, were the most abundant in the $PM_{2.5}$ and exhibited a high correlation coefficient with the mass concentration of $PM_{2.5}$. Seasonal variations of $PM_{2.5}$ showed a similar pattern to those of ionic and metallic species, with high concentrations during winter and spring. $PM_{2.5}$ also had a high correlation with the ionic species $NO_3{^-}$ and $NH_4{^+}$. In addition, $NH_4{^+}$ was highly correlated with $NO_3{^-}$. Through factor analysis, we identified four controlling factors, and determined the pollution sources using the United States Environmental Protection Agency(U.S. EPA) pollution profile. The first factor, accounting for 19.1% of $PM_{2.5}$ was attributed to motor vehicles and heating-related sources: the second factor indicated industry-related sources and secondary particles, and the other factors indicated soil, industry-related and marine sources. However, the pollution profile used in this study may be somewhat different from the actual situation in Korea, since it was obtained from US EPA. Therefore, to more accurately estimate the pollutants present in the air, a pollution profile for Korea should be produced.

• 한국미생물·생명공학회지
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• 제11권1호
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• pp.59-66
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• 1983

유포자 유산균인 Lactobacillus sporogenes 가 특이하게 균체외 $\beta$-galactosidase를 다량 생산하는 것을 발견하여 효소생산을 위한 영양요구성과 배양조건을 조사하였다. 영양요구성으로는 탄소원으로서 lactose 1%, 유기질소원으로서 peptone 1.5%, 무기질소원으로서 ammonium sulfate 0.2%, phosphorus 원으로 ammonium phosphate, dibasic을 0.8%. mineral은 potassium chloride 0.05%, ferric chloride 0.001% 첨가했을 때 최대의 효소생산을 나타내었다. 배지의 최적 initial pH는 7.0, 최적배양온도는 37$^{\circ}C$, aeration효과는 500$m\ell$용 진탕 flask에 배지량을 50~200$m\ell$ 주입, 140 strokes/min (진폭 7cm)으로 진탕 배양하였을 때 최고의 효소생산을 나타냈다. 상기의 최적조건에서 균체증식은 24시간 배양에 최고에 달하는 반면 $\beta$-galactosidase 생산은 균체내 효소생산의 경우 균체증식 속도와 비례적으로 증가하여 배양 24시간 전후에서 30U/$m\ell$로 최고치를 나타내고 균체외 효소는 배양 40시간 전후 즉, 균증식의 후기 정지기에서 38U/$m\ell$로 가장 높은 효소생산량을 보였다. 또한 fermentor 실험 역시 flask배양과 거의 비슷한 배양 양상을 보였으며 균체외 효소역가는 45U/$m\ell$로 진탕 flask 배양 결과보다 다소 양호하였다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제28권2호
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• pp.149-156
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• 2001

정보의 가치와 사용자의 정보획득 요구가 증대됨에 따라 특정 개인 위주의 서비스를 제공하는 정보검색 시스템의 필요성이 증대되고 있다. 그러나 현재의 정보검색 시스템들은 사용자의 선호도를 반영하고 편의성을 제공하는 면에서 매우 미흡한 점들이 많다. 따라서 본 논문에서는 적합성 정도에 따라 최적의 문서를 제공하기 위하여 사용자 위주의 문서순위결정 기법을 제안한다. 특정 개인의 선호도(preference)를 반영하기 위하여 사용자 프로파일(User Profile)을 구성 및 갱신하고, LSA(Latent Semantic Analysis)를 적용하여 적합율에 따라 문서의 순위를 결정한다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.739-740
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• 2001

37종의 주요 대사관련 유전자를 triplicate로 사용하여 DNA microarray를 제작하여 라이신 생산균주의 포도당과 원당을 탄소원으로 하여 배양시기에 따른 대사특성을 분석하였다. 포도당과 원당 사용시 C3, C4 대사산물의 변환에 관련된 anaplerosis에 관여하는 유전자의 발현변화가 매우 중요함을 파악할 수 있었다. 또한 배양시기에 따라 매우 특이적인 유선자 발현 양상을 보임을 학인할 수 있었다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 춘계학술대회 논문집
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• pp.98-101
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• 2001

In the present study, several design parameters of the flat-die extrusion process are investigated using the rigid-plastic finite element method. The effect of loaction of extrusion profile, arrangement of multiple extrusion profiles, and design of various die land has been investigated through the analysis. Several numerical examples of flat-die extrusion, such as C-section, multiple U- shape, and window guide extrusion, are analyzed. From the comparative study, the effect of design parameters is investigated. In each example, comparing the velocity distribution with that of the original design, it has been shown that the design modification affords much more uniform distribution of axial velocity

• 소성∙가공
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• 제10권7호
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• pp.551-557
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• 2001

In the present study, several design parameters of the flat-die extrusion process are investigated using the finite element method. The effects of the location of an extrusion profile, arrangement of multiple extrusion profiles, and the design of various die land have been investigated through the analysis. Several numerical examples of flat-die extrusion of such as C-section, multiple U-shape, and a window guide section, are analyzed. From the comparative study, the effect of design parameters is investigated. In each example, comparing the velocity distribution with that of the original design, it has been shown that the design modification affords more uniform distribution.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제51권4호
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• pp.165-173
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• 2002

The goal of this paper is optimal switching angle of switched reluctance motor drive system fur maximum energy ratio. A new magnetizing method with a low-frequency increasing the energy conversion ratio that is related to the efficiency of motor is proposed. As the results, it improved the efficiency about 2[%]. And a torque ripple is also sufficiently reduced compared with that of the conventional approach. In order tn start softly regardless of large ripple torque, the profile of phase current is predicted by the ANFIS, and current control mode was adapted when it is operated under the starting speed. Variable implementations en the fields will guarantee the more practical drive system.