• Journal of Embryo Transfer
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• v.22 no.2
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• pp.127-135
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• 2007

Components of cholesterol, glucose, minerals (selenium, Ca, p) and vitamin E in blood and milk were collected and analyzed weekly after calving from a total of 78 Holstein cows. In this study, we examined changes of chemical components, correlations among them, and factors affecting the changes. The results were summarized as follows; 1 Average levels of components in blood during 9 weeks after calving were 178.8 mg/dl, 62.3 mg/dl, 8.9 mg/dl, 7.4 mg/dl, 52.2 ng/ml, $10.3{\mu}g/ml$, 16.3 mg/dl, 15.3 ng/dl, and 15.8 mg/dl for cholesterol, glucose, Ca, P, selenium, vitamin E, BUN (blood urea nitrogen), MSe (milk selenium), and MUN (milk urea nitrogen), respectively. 2. The levels of cholesterol, glucose selenium, vitamin E and MSe tended to increase over time after calving. 3. The estimated correlations among components were negative(-) for cholesterol and p, positive for selenium, and BUN and also positive for BUN and MUN. Glucose showed positive correlations with P and MSe. 4. For factors influencing the changes of components, there were significant differences in glucose and selenium for age, in selenium and vitamin E for parity, in glucose and MSe for milk and in all components except Ca and selenium for feeding grass and hay, respectively. In urea nitrogen, age and parity only showed significant difference in MUN. In conclusion, the results were suggested that changes of blood and milk components depend on feeding during the postpartum. Especially, we could ascertain that selenium in blood and milk is the important factor for the dairy cows after parturition.

• Journal of the Korea Concrete Institute
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• v.17 no.1 s.85
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• pp.35-41
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• 2005

Recently, various fiber reinforced cementitious composites are used in order to solve problems of concrete as the brittleness breaking. Especially, in U.S.A., Europe, and Japan, ultra-high strength steel fiber reinforced cementitious composites(ultra-high strength SFRCC) with compressive strength in excess of 100 MPa were developed. However few studies have been investigated on the high-strength SFRCC in Korea. Therefore, in this paper, to make ultra-high strength SFRCC with the range of compressive strength 180MPa, it was investigated the constitute factors of ultra-high strength SFRCC influenced on the compressive strength. The experimental variables were water-binder ratio, replacement of silica fume, size and proportion of sand, type and replacement of filling powder, and using of steel fiber in ultra-high strength SFRCC. As a result, in water-binder ratio 0.20, we could make ultra-high strength SFRCC with compressive strength of 180MPa through using of silica fume, quartz sand with below 0.5mm filling powder and steel fiber.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.57
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• pp.283-303
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• 2013

When a trade conflict arises related to an officially supported export credit programme, The World Trade Organization(WTO), decides on whether the programme is a forbidden subsidy stipulated in the Agreement on Subsidies and Countervailing Measures(the ASCM Agreement). Korea was taken to the WTO panel two times for the export credit programme. One is the semiconductor case in 2002 and the other was the shipbuilding disputes in 2004. And, In 2012, the U.S. Commerce Department ruled K-SURE's export insurance for Korean refrigerator manufacturers as a forbidden subsidy even if the case was not taken to the WTO. This paper examines the significance of export credit programmes on the WTO ASCM Agreement and discusses how to operate these programmes so they would not infringe upon the Agreement by analyzing the actual cases of WTO subsidy conflicts that involved Korean enterprises in relation to export credit programmes for the purpose of determining the related issues and impacts. From this research the results were as follows: First, on whether export credit is a prohibited subsidy, the deciding factor was whether a benefit has been conferred to the beneficiary. On the presence of a benefit, the WTO panel used market benchmarks as the main criteria. Thus, official export credit agencies(ECAs) should be careful not to provide export credit support which had been granted to the beneficiary at better than market terms. Second, in the case of export credit, the special status of ECA as a public body receiving government support itself does not constitute a subsidy. However, caution must be taken not to provide export credit that may lead to WTO ASCM subsidy conflicts involving a certain exporter or industry by setting up clear and valid regulations and fair work processes in the operation of export credit programmes. Third, item (j) of Annex I cannot be interpreted reversely as this item is for interpreting the presence of a prohibited subsidy, not the presence of a benefit. Thus, an export credit program that confers a financial contribution, a benefit and specificity, could qualify as a prohibited subsidy. Fourth, ECAs not only have to maintain long-term account balance but also introduce additional measures to meet this long-term balance such as a clear and systematic premium system. Finally, export credit programmes that are not defined in item (j) of Annex I of the ASCM Agreement would not deemed as an prohibited export subsidy as long as the continued support of the programmes are not being forced.

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.5
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• pp.765-773
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• 1995

Effect of interfacial electrical conditions such as, the f potential of PET fiber and u-Fe203 particles, the stability parameter and potential energy of interaction on adhesion of a-Fe903 particles to PET fabric and their removal from the fabric, were investigated as functions of pH, electrolyte and ionic strength. The stability parameter, potential energy of interaction between a-Fe2O3 particles and PET fabric were calculated by using the heterocoagulation theory for a sphere-plate model The adhesion of a-Fe2O3 particles to PET fabric and their removal from PET fabric were carried out by using water bath shaker and Terg-O-Tometer under various solution conditions. The adhesion of a-Fe2O3 particles to the PET fabric and the removal of a-Fe2O3 particles from the PET fabric were biphasic and were maximum and minimum at pH 7~8, respectively. With high pH and polyanion electrolytes in solution, the adhesion of a-Fe2O3 particles to the PET fabric was low but effects of electrolytes on the removal of a-Fe2O3 particles from the PET fabric was small. The adhesion of a-Fe2O3 particles to the PET fabric and the removal of a-Fe2O3 Particles from the PET fabric were biphasic, and were lowest and highest at the ionic strength 1$\times$10-3, respectively. The adhesion of a-Fe2O3 particles to PET fabric was well related with the interfacial electrical conditions; it was negatively correlated with the f potentials of a-Fe2O3 Particles of its absolute value, the stability parameter and the maximum of total potential energy, while, the adhesion was not related with the t potentials of PET fiber itself. Therefore, the primary factor determining the adhesion of a-Fe203 particles to PET fabric may be the stability of dispersed particles caused by the electrical repulsion of particles. The removal of a-Fe203 particles from PET fabric was not related to such interfacial electrical conditions as the t potentials of PET fiber, the stability parameter and the maximum of total potential energy but removal was related to t potential of a-Fe203 particles.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.89-89
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• 2012

선행 연구에서는 m-Aramid와 p-Aramid의 Ring 방적사/Core 방적사 조건에 따른 방적사 물성에 대해서 연구하였는데, Ring 방적사의 혼용율, 스핀들 속도에 따른 물성 변화, Core 방적사의 공정특성에 따른 방적성, 피복성에 대해 연구를 하였다. 그 결과 Ring 방적사의 경우 m-Aramid의 혼용율이 증가할수록 사 강도는 증가하는 경향을 확인할 수 있었고, 스핀들 속도가 증가함에 따라서 불균제도 및 사결점이 다소 증가하였다. Core 방적사의 경우 Core에 p-Aramid를 사용했을 때 방적성과 피복성은 양호 하였지만, 강한 p-Aramid에 의해 톱 롤러코트의 마모가 되는 경향을 볼 수 있었다. 본 연구에서는 Ring 방적사/Core 방적사에 대해 좀더 구체적인 제조공정조건에 의한 물성 변화를 알아보았고, 좀더 다양한 섬유소재를 적용해서 제조하였다. Ring 방적사의 경우 p-Aramid 혼용율에 따른 실험을 하였는데, m-Aramid에 대한 p-Aramid의 혼용율을 0%, 5%, 10%, 20% 로 하여 Ne30을 제조하였으며, Core 방적사는 Core를 p-Aramid 200D로 하고 Sheath를 Cotton으로 하여 Core 공급속도비 (1.06, 1.10, 1.14), 연계수(T/M, 3.8, 4.0, 4.2), Sheath/Core 혼섬율(70/30, 60/40, 50/50)에 따라 제조하였다. 추가로 Core 방적사는 Sheath에 Cotton 대신 FR-Rayon과 선염 Cotton을 사용하여 각각 Sheath/Core 혼섬율 70/30의 비율로 방적사를 제조하여 다양한 소재에 따른 방적사의 물성을 측정하였다. 제조된 시험 원사들의 측정 물성은 번수(Ne), 균제도(U%), 사결점(IPI), 강력(cN), 신도(%), 비강도(cN/Tex) 등이며, 편직을 통해 편성물의 외관을 확인하였다. Ring 방적사의 경우 p-Aramid의 혼용율이 증가할수록 강도는 완만하게 증가한 반면, 신도는 급격하게 감소하였다. Cotton Core 방적사의 경우는 공급속도비가 높아질수록 균제도가 높아지는 것을 볼수 있었고, Core 공급속도비가 높아질수록 외관상 피복도는 높아진 것을 볼 수 있었다. 이렇게 연구된 Aramid 소재를 이용한 방적사 제조 공정조건변화에 따른 물성 및 피복도 결과는 기존 방적업체에서 Aramid를 이용한 방적사 제조시에 공정조건을 확보하는데 도움이 될 것이라 생각되며, 방적사 시제품 생산시에 발생할 수 있는 시행착오를 줄임으로써 시제품 제조를 위한 생산비용의 Loss를 절감할 수 있을 것으로 예상된다.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.11
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• pp.1649-1656
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• 2015

Gene expression profiling has offered new insights into postmortem molecular changes associated with meat quality. To acquire reliable transcript quantification, high quality RNA is required. The objective of this study was to analyze integrity of RNA isolated from chicken skeletal muscle (pectoralis major) and its capability of serving as the template in quantitative real-time polymerase chain reaction (qPCR) as a function of postmortem intervals representing the end-points of evisceration, carcass chilling and aging stages in chicken abattoirs. Chicken breast muscle was dissected from the carcasses (n = 6) immediately after evisceration, and one-third of each sample was instantly snap-frozen and labeled as 20 min postmortem. The remaining muscle was stored on ice until the next rounds of sample collection (1.5 h and 6 h postmortem). The delayed postmortem duration did not significantly affect $A_{260}/A_{280}$ and $A_{260}/A_{230}$ ($p{\geq}0.05$), suggesting no altered purity of total RNA. Apart from a slight decrease in the 28s:18s ribosomal RNA ratio in 1.5 h samples (p<0.05), the value was not statistically different between 20 min and 6 h samples ($p{\geq}0.05$), indicating intact total RNA up to 6 h. Abundance of reference genes encoding beta-actin (ACTB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine-guanine phosphoribosyltransferase (HPRT), peptidylprolylisomerase A (PPIA) and TATA box-binding protein (TBP) as well as meat-quality associated genes (insulin-like growth factor 1 (IGF1), pyruvate dehydrogenase kinase isozyme 4 (PDK4), and peroxisome proliferator-activated receptor delta (PPARD) were investigated using qPCR. Transcript abundances of ACTB, GAPDH, HPRT, and PPIA were significantly different among all postmortem time points (p<0.05). Transcript levels of PDK4 and PPARD were significantly reduced in the 6 h samples (p<0.05). The findings suggest an adverse effect of a prolonged postmortem duration on reliability of transcript quantification in chicken skeletal muscle. For the best RNA quality, chicken skeletal muscle should be immediately collected after evisceration or within 20 min postmortem, and rapidly preserved by deep freezing.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.7
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• pp.490-499
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• 2017

In recent years, the introduction of complex systems analysis based on various variables has become more active in order to identify and analyze complex problems of Modern Society. Prediction of patients' spatial perception and usability according to the spatial arrangement of the outpatient department is a very important factor for providing high quality hospital service. For objective analysis, the standard program procedure and analysis index for the diseases of the elderly were prepared and the uniformity of the atmospheric space was evaluated through heat map analysis and quantitative analysis. In this study, 73 cells were installed and simulated to analyze the uniformity of the four alternatives according to the change of the arrangement of the medical care space, receiving space, and consultation space using the complex system analysis method for the nursing hospitals. The resulting density was derived. The results are as follows. 1)The layout of the reception space has the greatest influence on the total spatial density of the waiting space. 2) The uniformity of the waiting space can be increased by separating the examination space and the examination space. 3)The closer the location of the receiving space is from the entrance, the greater the density of the waiting space. Finally, this study applied discrete event simulation to the evaluation of uniformity of atmosphere space, and proved that the actor - based model can be utilized for utilization and evaluation as spatial analysis methodology.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.999-1006
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• 2015

The endo-polygalacturonase gene (endo-pgaA) was cloned from DNA of Aspergillus niger SC323 using the cDNA synthesized by overlapping PCR, and successfully expressed in Saccharomyces cerevisiae EBY100 through fusing the α-factor signal peptide of yeast. The fulllength cDNA consists of 1,113 bp and encodes a protein of 370 amino acids with a calculated molecular mass of 38.8 kDa. After induction by galactose for 48 h, the activity of recombinant endo-PgaA in the culture supernatant can reach up to 1,448.48 U/mg. The recombinant protein was purified to homogeneity by ammonium sulfate precipitation and gel filtration column chromatography and subsequently characterized. The optimal pH and temperature of the purified recombinant enzyme were 5.0 and 50℃, respectively. The Michaelis-Menten constant (K_m) and maximal velocity (V_max) of the enzyme for pectin were 88.54 μmol/ml and 175.44 μmol/mg/min, respectively. The enzyme activity was enhanced by Ca²⁺, Cu²⁺, and Na⁺, and strongly inhibited by Pb²⁺ and Mn²⁺. The pectin hydrolysates were mainly galacturonic acid and other oligo-galacturonates. Therefore, these characteristics suggest that the recombinant endo-PgaA may be of potential use in the food and feed industries.

• The Journal of the Korea Contents Association
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• v.14 no.7
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• pp.253-270
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• 2014

Public awareness campaign is the core factor of the national suicide prevention strategies. Korea's current campaign needs to be more systematically developed. This study analyzed the national-level campaigns for suicide prevention of New Zealand, USA, Ireland, Scotland, and Australia whose campaigns were known to be successful, and compared them with those of Korea. With the analysis framework based on the 'Guideline for Effective Health Communication Campaigns' by the U.S. Department of Health and Human Services, we examined the campaigns from the dimensions of (1) clarity of goals, (2) appropriacy of targeting strategies, (3) suitability of messaging strategies, and (4) efficiency of performance. The results show that effective campaigns for suicide prevention have the following common factors: (1) campaign appellations which include clear goals, (2) targeting at risk groups considering social contexts, (3) slogans which contains specific action guidelines, and (4) close relationships between public and private sectors. On this basis, future directions for more effective suicide prevention media campaigns in Korea are discussed.

• Journal of Korean Society for Atmospheric Environment
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• v.28 no.2
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• pp.211-221
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• 2012

The Ministry of Environment will enforce air quality standards for $PM_{2.5}$ in 2015 because it affects human health as well as climate change and brings about other adverse effects. Until recently, even though a number of researches have reported $PM_{2.5}$ emissions according to sources, they have not precisely considered the emission factors correspondent to each source for emission estimation. For the sake of establishing $PM_{2.5}$ emission inventories, this study was undertaken using activity data of each source taken from CAPSS (Clean Air Policy Support System) multiplied by each emission factor of U.S. EPA Speciate or EEA CORINAIR. The 2008's total annual $PM_{2.5}$ emission (73.971 ton/yr) can be apportioned into five sources, such as fuel combustion facilities (62.2%), mobiles (33.8%), production processes (3.2%), fires (0.4%), and waste treatments (0.3%). The results show that fuel combustion facilities and mobiles are the predominant sources of $PM_{2.5}$, and they should be taken into great account in establishing $PM_{2.5}$ standards. In addition, it is necessary and urgent to develop effective measures for reduction of $PM_{2.5}$ emissions from those two main sources as well.