• Title/Summary/Keyword: Two-cell

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Performance Predictions of the Planar-type Solid Oxide Fuel Cell with Computational Flow Analysis (I) - Isothermal Model - (유동 해석을 이용한 평판형 고체 산화물 연료전지의 성능 특성 분석 (I) - 등온 모델 -)

  • Hyun, Hee-Chul;Sohn, Jeong L.;Lee, Joon-Sik;Ro, Sung-Tack
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.27 no.5
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    • pp.635-643
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    • 2003
  • Parametric study for the analysis of performance characteristics of a planar -type solid oxide fuel cell(SOFC) using computational flow analysis is conducted. A planar -type SOFC, which is composed by two gas channels (fuel and ai.) and one set of anode-electrolyte-cathode assembly, is modeled as a two -dimensional isothermal case. Results of computational analysis of flow field including distributions of mass fractions in gas channels are used to the performance analysis of the fuel cell. Flow analysis makes it possible to consider current density distributions along the length of the cell in the process of performance analysis of the SOFC. As results of parametric study, it is found that the mole fraction of fuel at the inlet of fuel channel, operating pressure and temperature are closely related to the performance characteristics of SOFC.

Flow Cytometric Analysis of Human Lysozyme Production in Recombinant Saccharomyces cerevisiae

  • Peterson Marvin S.;Kim Myoung-Dong;Han Ki-Cheol;Kim Ji-Hyun;Seo Jin-Ho
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.1
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    • pp.52-55
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    • 2002
  • Flow cytometric techniques were used to investigate cell size, protein content and cell cycle behavior of recombinant Saccharomyces cerevisiae strains producing human lysozyme (HLZ). Two different signal sequences, the native yeast $MF\alpha1$ signal sequence and the rat $\alpha-amylase$ signal sequence, were used for secretion of HLZ. The strain containing the rat $\alpha-amylase$ signal sequence showed a higher level of internal lysozyme and lower specific growth rates. Flow cytometric analysis of the total protein content and cell size showed the strain harboring the native yeast signal sequence had a higher total protein content than the strain containing the rat $\alpha-amylase$ signal sequence. Cell cycle analysis indicated that the two lysozyme producing recombinant strains had an increased number of cells in the $G_2+M$ phase of the yeast cell cycle compared with the host strain SEY2102.

Mouse Tumor Necrosis Using Photodynamic Therapy (광역학적 치료법을 이용한 쥐의 악성종양 괴사)

  • 임현수;변상현
    • Journal of Biomedical Engineering Research
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    • v.25 no.1
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    • pp.49-55
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    • 2004
  • In this paper, we investigated the effects of the photodynamic therapy(PDT) for the tumor mass in mice. In the experimental method, we divided the mice into two control and test group which HepG2 and HeLa cell line induced cancer mass in mice. Photofrin was administered to the tumor-bearing mouse, followed 30 hours later by 630nm and 650nm laser light exposure. After photodynamic therapy we analyzed the two mice group for the tumor mass size, tumor growth, tumor cell necrosis, pathological anatomy change. According to the results, tumor cell necrosis was shown in the tissues which the reduce size of tumor and tumor cell necrotic change according to the irradiation time and light dose amount. The considerable difference, however, between the 630nm and 650nm wavelength was not found for the tumor cell necrotic change and other damage of normal tissue was not found.

A novel method for cell counting of Microcystis colonies in water resources using a digital imaging flow cytometer and microscope

  • Park, Jungsu;Kim, Yongje;Kim, Minjae;Lee, Woo Hyoung
    • Environmental Engineering Research
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    • v.24 no.3
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    • pp.397-403
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    • 2019
  • Microcystis sp. is one of the most common harmful cyanobacteria that release toxic substances. Counting algal cells is often used for effective control of harmful algal blooms. However, Microcystis sp. is commonly observed as a colony, so counting individual cells is challenging, as it requires significant time and labor. It is urgent to develop an accurate, simple, and rapid method for counting algal cells for regulatory purposes, estimating the status of blooms, and practicing proper management of water resources. The flow cytometer and microscope (FlowCAM), which is a dynamic imaging particle analyzer, can provide a promising alternative for rapid and simple cell counting. However, there is no accurate method for counting individual cells within a Microcystis colony. Furthermore, cell counting based on two-dimensional images may yield inaccurate results and underestimate the number of algal cells in a colony. In this study, a three-dimensional cell counting approach using a novel model algorithm was developed for counting individual cells in a Microcystis colony using a FlowCAM. The developed model algorithm showed satisfactory performance for Microcystis sp. cell counting in water samples collected from two rivers, and can be used for algal management in fresh water systems.

Basal cell adenoma of parotid gland: two case reports and literature review

  • Sungyeon Yoon;Yesol Kim;Suk-Ho Moon
    • Archives of Craniofacial Surgery
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    • v.24 no.4
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    • pp.179-184
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    • 2023
  • Most of salivary tumors are benign in nature and are typically diagnosed and classified based on their histopathological presentation. Basal cell adenoma of the salivary glands is a rare, benign disease accounting for 1% to 3% of salivary gland tumors. Despite its low incidence, basal cell adenoma is the third most common benign tumor of the salivary gland after pleomorphic adenoma and Warthin's tumor. It usually appears as a firm and slow-growing mass. Due to the prognosis, differential diagnosis with basal cell adenocarcinoma, adenoid cystic carcinoma and basaloid squamous cell carcinoma is required. In this report, we present two cases; a 62-year-old woman who presented with an asymptomatic, and slow-growing mass and a 64-year-old woman with a static-sized mass in the parotid gland. In both cases, the mass was completely excised, postoperative pathology reports confirmed the diagnosis of basal cell adenoma. We also review the literature and discuss this rare entity.

황환원 세균의 quorum-sensing 유사 현상

  • Park, Ji-Eun;Jang, Deok-Jin
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.545-548
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    • 2001
  • Microbiologically influenced corrosion (MIC) of metal is common in the natural environment and sulfate reducing bacteria are representative microorganisms for MIC. We found that biofilm fomlation by SRB on the metal surface might be controlled by quorum sensing, which is a cell density dependent regulation of cell metabolism. As cell free culture fluids (spent media) of Desulfovibrio vulgaris and D. desulfuricans were tested for quontrn sensing related test strains, it was found that spent media of two SRB induced increased luminescence of Vibrio harveyi BB886 (sensor 1+, sensor 2-) and BB170 (sensor 1-, sensor 2+). Quorum activities of D. vulgaris and D. desulfuricans appeared to be parallel to growth patterns, i.e., it was low in the lag phase, highly increased in the exponential phase, and reached maximum in the stationary phase. Interestingly, however, luminescence of V. harveyi BB886 and BB170 induced by a unit cell mass of the SHB showed a maximal peak in the late lag phase. Hence, it was suspected that quorum sensing of these two SHB play unknown roles in shifting cells from dormant to growth stages.

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Observation of Water Consumption in Zn-air Secondary Batteries

  • Yang, Soyoung;Kim, Ketack
    • Journal of Electrochemical Science and Technology
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    • v.10 no.4
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    • pp.381-386
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    • 2019
  • Zn-air battery uses oxygen from the air, and hence, air holes in it are kept open for cell operation. Therefore, loss of water by evaporation through the holes is inevitable. When the water is depleted, the battery ceases to operate. There are two water consumption routes in Zn-air batteries, namely, active path (electrolysis) and passive path (evaporation and corrosion). Water loss by the active path (electrolysis) is much faster than that by the passive path during the early stage of the cycles. The mass change by the active path slows after 10 h. In contrast, the passive path is largely constant, becoming the main mass loss path after 10 h. The active path contributes to two-thirds of the electrolyte consumption in 24 h of cell operation in 4.0 M KOH. Although water is an important component for the cell, water vapor does not influence the cell operation unless the water is nearly depleted. However, high oxygen concentration favors the discharge reaction at the cathode.

Improvement of Efficiency about $TiO_2$ Layer Multi-dividing Effect in Dye-sensitized Solar Cell (염료감응형 태양전지의 $TiO_2$ Layer 다분할 효과에 따른 효율 향상 연구)

  • Son, Min-Kyu;Seo, Hyun-Woong;Lee, Kyoung-Jun;Hong, Ji-Tae;Kim, Hee-Je
    • 한국신재생에너지학회:학술대회논문집
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    • 2008.05a
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    • pp.425-427
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    • 2008
  • Active area of dye-sensitized solar cell (DSSC) has an effect on the efficiency of DSSC. As the active area increases, the efficiency goes down in a general way. This is caused by the increase of internal resistance in DSSC. The internal resistances are related to various resistant elements. The charge transfer processes at Pt counter electrode and the sheet resistance of TCO are two of these resistant elements. In this study, we try to divide the active area into several small sections in a large sized cell to reduce these two internal resistant elements. As a result, we find out that the fill factor is increased and then the conversion efficiency is improved as the number of dividing active area into several small sections is increased.

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Efficient Ρ-median approach to GT cell formation (GT 셀 형성을 위한 효율적 Ρ-median 접근법)

  • Won, Youkyung
    • Proceedings of the Korean Operations and Management Science Society Conference
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    • 2000.04a
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    • pp.40-43
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    • 2000
  • This paper is concerned with development of an efficient Ρ-median approach applicable to large cell formation(CF) problems. A two-phase methodology that seeks to minimize the number of exceptional elements is proposed. In phase I, two efficient Ρ-median formulations which contain fewer binary variables than existing Ρ-median formulations are constructed. These make it possible to implement large CF problem within reasonable computer runtime with commercially available linear integer programming codes. Given the initial cell configuration found with the new p-median formulations, in phase II bottleneck machines and parts are reassigned to reduce the number of exceptional elements. This procedure has the flexibility to provide the cell designer with alternative solutions. Test results on large CF problems show a substantial efficiency of the new Ρ-median formulations.

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Ethanolic Extract from Derris scandens Benth Mediates Radiosensitzation via Two Distinct Modes of Cell Death in Human Colon Cancer HT-29 Cells

  • Hematulin, Arunee;Ingkaninan, Kornkanok;Limpeanchob, Nanteetip;Sagan, Daniel
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1871-1877
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    • 2014
  • Enhancing of radioresponsiveness of tumors by using radiosensitizers is a promising approach to increase the efficacy of radiation therapy. Recently, the ethanolic extract of the medicinal plant, Derris scandens Benth has been identified as a potent radiosensitizer of human colon cancer HT29 cells. However, cell death mechanisms underlying radiosensitization activity of D scandens extract have not been identified. Here, we show that treatment of HT-29 cells with D scandens extract in combination with gamma irradiation synergistically sensitizes HT-29 cells to cell lethality by apoptosis and mitotic catastrophe. Furthermore, the extract was found to decrease Erk1/2 activation. These findings suggest that D scandens extract mediates radiosensitization via at least two distinct modes of cell death and silences pro-survival signaling in HT-29 cells.