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A Case Study of Display Design of Space 'O'sulloc Teahouse' from the Point of Sequence Narrative (시퀀스 내러티브 관점에 따른 '오설록 티하우스' 공간의 제품 전시 디자인 사례 연구)

  • Yang, Hyeon-Jeong;Lee, Hyunsoo
    • Korean Institute of Interior Design Journal
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    • v.23 no.1
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    • pp.61-68
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    • 2014
  • Recently, there have been increasing attempts to pursue and express feelings such as sensibilities, emotions, and impressiveness in commercial spaces. One of such methods is to apply 'storytelling' to space designs. Applicability of storytelling to a space suggests that the contents of a space can be expressed through various mediums. Portraying events and situations through a single time continuity of a story is referred to as 'narrative'. The movement of users and sequence of contents are determined by a narrative. It provides different storytelling and a sense of place to each space through various roles, such as wide association, engraving, and image formation. A narrative can lead users to engage in different perceptions and behaviors even in spaces with the same content. Thus, this study is intended to examine the impact of space marketing in line with design narratives, assuming that narratives of commercial space designs will influence the formation of brand identity. The research methods are as follows. First, the definition of narratives in space design was established by examining narrative architectures. Second, design analysis tools for commercial spaces were established from the perspective of narratives through preceding studies. Third, the design narratives of different shops under the same brand were comparatively analyzed through a case study. To carry out a case study, a commercial space of 'O'sulloc' was selected, and its brand identity was studied from the narrative standpoint. The case study involved interior designs of 7 road shops of 'O'sulloc.' Among the 7 road shops, two of them with the biggest difference in design narratives were selected, and an observation survey was done on the users as a second analysis. Through the observation survey, actual design narrative experience was analyzed in 4 steps of introduction, development, turn, and conclusion. The findings are as follows. The design method of each shop varied, and different design elements were emphasized. Among various elements, the ones that reflect the brand identity of 'O'sulloc' the best were logo, product, and shape. During the process of narratives, the characteristics of each shop and user recognition and behavior varied depending on the degree of emphasis on a particular element. It suggests that space design narratives can influence the formation of brand identity. This study provides ideal directions of developing space designs necessary for forming brand identity from the standpoint of Korean traditional culture modernization. Future studies could discuss the economic feasibility of such designs.

Production of Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid by Pseudomonas sp. HJ (Pseudomonas sp. HJ에 의한 Poly(Hydroxybutyric-Co-Hydroxyvaleric) Acid의 생산)

  • 손홍주;민관필이상준
    • KSBB Journal
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    • v.10 no.4
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    • pp.349-356
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    • 1995
  • To produce PHA(polyhydroxyalkanoic acid) from microbr, dozens of microorganism have been screened from sewage sludge. Selected a strain HJ out of 50 strains of PHA producing bacteria has a capability of accumulating large amounts of PHB/HV copolymer when grown in batch culture with a single carbon source (glucose) that was not generally considered as precursor of hydroxyvalerate monomer unit. The strain HJ was identified as the genus Pseudomonas with respect to morphological, cultural, and biochemical characteristics. The optimal temperature and pH for cell growth were $37^{\circ}C$ and 7.0. The optimal medium compositions for cell growth were glucose 1% as a carbon source, (NH4) 2SO4 0.2% as a nitrogen source, K2HPO4 0.3%, and KH2PO4 0.45%. TO investigate she optimal condition for PHA production two-step cultivation method was employed. PHA production was inducted by deficiency of NH4+, SO4-2, Mg+2. Besides carbon source, deficiency of all nutrients stimulated PHA productivity but deficiency of NH4+ stimulated the most HV monomer content. The highest PHA production was C/N molar ratio 95.2. Pseudomonas sp. HJ was also able to pyoduc PHB/HV copolymer when cultivated on alkane, alkanoate, alcohol as carbon sources. The contents of PHA and she proportions of hydroxyvalerate monomer units varied depending on the carbon sources. Especially Pseudomonas sp. HJ was able to incorporate hydroxyvalerate into PHB/HV to level as high as from 49 to 74 mol% when grown in a medium containing hexadecane and propionate. The purified PHA was identified PHB/HV copolymer by HNMR analysis.

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Recovery of Intracellular Enzyme Using Phage Lysogen and Expanded Bed Adsorption (Phage Lysogen과 Expanded Bed Adsorption을 이용한 세포내 효소의 회수)

  • 장우진;윤현식구윤모
    • KSBB Journal
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    • v.11 no.6
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    • pp.663-668
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    • 1996
  • In the production of recombinant protein using E. coli, phage lysogen system can be usefully applied for simultaneously achieving protein production at high cell concentration and recovery by cell disruption in the same bioreactor. A major drawback of this system is that the intracellular product and complex broth components are mixed together in culture broth and hence purification efficiency is reduced. With the E. coli double-lysogen system, the expanded bed adsorption is very useful because the pretreatment processes in a routine bioseparation process can be done in a single column operation, and therefore may contribute towards lowering the operating cost of overall recovery/purification process. In the operation of EBA, it has been observed that the change in broth feed volume does not influence much the protein recovery in a tested range. The amount of protein adsorption per mL of resin was increased from $3.44{\times}106unit to 5.28{\times}106unit$ by doubling the column length. By two-fold increase of the column diameter, the ratio of protein concentration in eluent to that in feed was increased from 0.8 to 2.1. It is concluded from the present investigation that the increase of column length and diameter is necessary to enhance the protein adsorption amount per volume of resin and protein concentration in the eluent. The development of resins with various physical properties will be necessary for more extensive application of EBA.

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Fungicidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient against Trichophyton mentagrophytes, Candida albicans and Aspergillus niger (Ortho-phenylphenol을 주성분을 하는 훈증소독제의 Trichophyton mentagrophytes, Candida albicans 그리고 Aspergillus niger에 대한 살진균 효과)

  • Park, Eun-Kee;Lee, Soo-Ung;Cho, Ki-Yung;Kim, Yongpal;Yoo, Chang-Yeol;Kim, Suk;Lee, Hu-Jang
    • Journal of Environmental Health Sciences
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    • v.40 no.3
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    • pp.255-263
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    • 2014
  • Objectives: This study evaluated the fungicidal efficacy of a fumigant containing 20% ortho-phenylphenol against Trichophytone mentagrophytes (T. mentagrophytes), Candida albicans (C. albicans) and Aspergillus niger (A. niger). Methods: Five replicates of each carrier were contaminated by depositing 0.05 mL of each fungal suspension. After drying, two carriers without exposure to the fumigant and three carriers with exposure to the fumigant were left in a sealed room ($25m^3$) at $21{\pm}0.5^{\circ}C$ and $60{\pm}10%$ relative humidity for 15 hours. Immediately after removal from the test room, each carrier was transferred into recovery diluent and suspended, diluted and inoculated. After incubation, the numbers of each colony were counted, and the parameter values (N, T, d) were calculated. Results: The working culture suspension number (N value) of T. mentagrophytes, C. albicans and A. niger were $1.0{\times}10^8$, $1.2{\times}10^8$ and $5.7{\times}10^7CFU/mL$, respectively. All the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of fungal test suspensions by pour plate method), 0.5N2 (the number of fungal test suspensions by filter membrane method) and 0.5N1, respectively. In addition, all mean numbers of test strains recovered on the control-carriers (T value) were over $10^6CFU/mL$. For the fungicidal effect of the fumigant, all numbers of fungal reductions after exposure of the fumigant (d value) were 4 logCFU/mL. Conclusions: The present study showed that fumigant containing 20% ortho-phenylphenol has effective fungicidal activity against T. mentagrophytes, C. albicans and A. niger.

Determination of the Optimal Concentration of Fetal Bovine Serum for the Growth of Two Insect Cell and Viruses (두 가지 곤충 세포주에 대한 배양 및 바이러스 증식을 위한 최적 FBS 농도 결정)

  • Lee, Jae-Kyung;Koo, Hyun-Na;Woo, Soo-Dong
    • Korean journal of applied entomology
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    • v.46 no.2
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    • pp.319-324
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    • 2007
  • To determine the optimal concentration of fetal bovine serum (FBS) on the growth of insect cells and the multiplicity of viruses, the growth of cells (Sf21 and Bm5) and viruses were examined on the various concentrations of FBS. In view of the viability, growth speed, proliferation of cells and the amount of FBS, the most proper concentration for the cell culture were 7% and 5% for Sf21 and Bm5, respectively. The multiplicity of viruses at the various concentrations of FBS was similar in both cell lines at 5 days post-infection (p.i.). However, it differed significantly at 2 and 3 days p.i. The proper concentration of FBS were 10% and 3% for Sf21 at 2 and 3 days p.i., respectively, and 5% for Bm5 at both 2 and 3 days p.i. These results suggested that the optimal concentration of FBS should be determined according to the used cell lines and viruses for their optimum production.

Genealogy of the Rainbow Stripe in Ethnic Costumes in East Asia (동부아시아 민족복식 색동계보)

  • Cho, Woo-Hyun;Kim, Mi-Jin
    • Journal of the Korean Society of Costume
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    • v.60 no.4
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    • pp.113-125
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    • 2010
  • This study is purpose to trace a genealogy of Rainbow stripe in 30 ethnic costumes in East Asia. And with through comparative views between Korean and the other minority that is shown a bilateral relation of rainbow stripe in their costume, we make sure the unique character of rainbow stripe in Korean traditional costume. The stripe in the costumes was generally used on sleeves with 3~6 patches. There were 10 types of methods for making the stripe including sewing. Weaving and sewing with trimming was also frequently used either and the stripe by weaving with multicolored yarns were mostly found in the southern region of china. Black, blue and red were frequently used in the costume as a main color that was contrasted with rainbow stripe and especially, black was mostly used. Korean preferred bright colors as a main color. Contents of the genealogy of the multicolored stripe in ethnic costume in East Asia are followed. The 28 ethnic groups who used the stripe in their dress except Korean, the Mans, Mongo people and Tibetan were located in the southern region of East Asia. And the other ethnic groups distributed in the northwest and northeast region of East Asia. The distribution of the rainbow stripe in the costume could be grouped into two sections: the southern region people and Korean-the Mongol people-the Tus- the Zangs group. And the latter group was shown strong relation with the culture of Korean's rainbow stripe costume. 11 ethnic peoples including Korean, the Vis, the Miaos, the Tus, the Mongol people, the Chaoxians, the Zangs, the Lahus, the Jinuos, the Hanis, the Luobas and the Dulongs, were saliently used the stripe in their costume. The stripe in Japanese costume was judged that was not a kind of the rainbow stripe was shown the other ethnic groups, was a color arrangement by layered dress or geometrical pattern. From above, we could recap a particular characteristic of the rainbow stripe in Korean traditional costume. Many colors were used in the stripe and bodies than the other ethnic people and the color was bright. In many cases, a color of patch at the point of armhole was red and Black color was not used in the stripe. The width of patch was a relatively narrow and regular. It has shown that the rainbow stripe in Korean traditional costume was organized independently.

Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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Inflammatory Mediators Modulate NK Cell-stimulating Activity of Dendritic Cells by Inducing Development of Polarized Effector Function

  • Kim, Kwang-Dong;Choi, Seung-Chul;Lee, Eun-Sil;Kim, Ae-Yung;Lim, Jong-Seok
    • IMMUNE NETWORK
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    • v.7 no.3
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    • pp.133-140
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    • 2007
  • Background: It is well established that cross talk between natural killer (NK) cells and myeloid dendritic cells (DC) leads to NK cell activation and DC maturation. In the present study, we investigated whether type 1-polarized DC (DC1) matured in the presence of IFN-${\gamma}$ and type 2-polarized DC (DC2) matured in the presence of PGE2 can differentially activate NK cells. Methods: In order to generate DC, plastic adherent monocytes were cultured in RPMI 1640 containing GM-CSF and IL-4. At day 6, maturation was induced by culturing the cells for 2 days with cytokines or PGE2 in the presence or absence of LPS. Each population of DC was cocultured with NK cells for 24 h. The antigen expression on DC was analyzed by flow cytometry and cytokine production in culture supernatant was measured by ELISA or a bioassay for TNF-${\alpha}$ determination. NK cell-mediated lysis was determined using a standard 4h chromium release assay. Results: DC2, unlike DC1, had weak, if any, ability to induce NK cell activation as measured by IFN-${\gamma}$ production and cytolytic activity. DC2 were weakly stimulated by activated NK cells compared to DC1. In addition, IFN-${\gamma}$-primed mature DC appeared to be most resistant to active NK cell-mediated lysis even at a high NK cell/DC ratio. On the other hand, PGE2-primed DC were less resistant to feedback regulation by NK cells than IFN-${\gamma}$-primed mature DC. Finally, we showed that the differential effect of two types of DC population on NK cell activity is not due to differences in their ability to form conjugates with NK cells. Conclusion: These results suggest that different combinations of inflammatory mediators differentially affect the effector function of DC and, as a result, the function of NK cells, eventually leading to distinct levels of activation in adaptive immunity.

A Study on the Analysis of Cause and Effect Relationship between Korea Image Attributes and Korea Image Making in Uzbekistan (우즈베키스탄에서의 한국 이미지 속성과 한국 이미지 메이킹의 인과관계 분석)

  • Ryu, Ki-Hwan;Park, Myung-Chan
    • International Commerce and Information Review
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    • v.15 no.1
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    • pp.161-183
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    • 2013
  • The essential purpose of this study is to exemplify empirically some relations of between Korea image attributes and Korea image making in Uzbekistan. This study is for providing basically empirical analysis for intensifying Korea's economic capacity based on Korean image in Uzbekistan and for offering any kinds of implications for the entry and management activities of Korean enterprises in world-wide business. This study is organized in two stages. First, the study model is designed by reappraising relevant theories, previous studies and the current investment qualifications concerning the country image attributes and image making. Second, the survey of Uzbekistan people who are living in South Korea is accomplished by collecting questionnaires. SPSS 15.0 for windows is being activated for correlation, reliability, validity and finally the statistical method of structural equation modeling is utilized to testing the hypothesis by AMOS 7.0 for windows. In conclusion partially, Korea image attributes: economic capability, popular culture, national traits and enterprise competence are proved to influence positively to Korea image making: recognition, attractiveness and confidence which is considered generally by Uzbekistan people.

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Comparative Study on Seasonal Festival and Food Culture among the Korea, China and Japan (한.중.일 세시풍속과 세시음식(歲時飮食)에 대한 비교)

  • Shin, Mee-Kyung;Chung, Hee-Chung
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.3
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    • pp.277-293
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    • 2008
  • We conducted a consensual, expansive, and successive study to compare cultural differences and similarities between Korean, Chinese and Japanese's Seasonal Festivals and Foods documentarily. It showed interesting results that the three countries had celebrated with similar meanings, and shared similar events and special foods. Seasonal Festivals happen throughout the year sequentially: the New Year Day, January $15^{th}$(first full moon festival), March $3^{rd}$(double three day), May $5^{th}$(double five day), July $7^{th}$(double seven day) and finally the Year Farewell Festival. While, the festival of a royal birthday of Buddha happens on April $8^{th}$ in both countries: Korea and Japan. There is also one of the big festivals called the harvest moon festival on August $15^{th}$, and this event celebrates with special foods both in Korea and China. On December $23^{th}$, it is a special day for both Chinese and Japanese, but they celebrate the day with different meanings to it. Three countries have a special seasonal event celebrated each other. Koreans have a special event called Sambok to overcome summer with special food during a middle of June to the beginning of July. Chinese have a special event for overcoming winter with soup on December $8^{th}$. Japanese have a special event with rice cake to safety on January $11^{th}$. On these seasonal festivals, it is different to note that two different kinds of calendars are used. The lunar calendar is used by Koreans and Chinese whereas the solar calendar is used by Japanese. Because of the similarity in Buddhism, and agricultural industry, and especially sharing Chinese Characters in words, these three countries have in common in many ways to celebrate Seasonal Festival, and it is very unique custom in the world. Nowadays; however, these traditional events and special foods are changing in more simplified version and almost disappearing in all three countries. Therefore, we suggest that the Seasonal Festivals and Foods should be more emphasized in flourishing and exchanging between Korea, China and Japan.

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