Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Determination of the Optimal Concentration of Fetal Bovine Serum for the Growth of Two Insect Cell and Viruses

두 가지 곤충 세포주에 대한 배양 및 바이러스 증식을 위한 최적 FBS 농도 결정

  • Lee, Jae-Kyung (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Koo, Hyun-Na (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Woo, Soo-Dong (School of Applied Life Science & Environment, College of Agriculture Life & Environment Science, Chungbuk National University)
  • 이재경 (충북대학교 농업생명환경대학 응용생명환경학부) ;
  • 구현나 (충북대학교 농업생명환경대학 응용생명환경학부) ;
  • 우수동 (충북대학교 농업생명환경대학 응용생명환경학부)
  • Published : 2007.08.30
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Abstract

To determine the optimal concentration of fetal bovine serum (FBS) on the growth of insect cells and the multiplicity of viruses, the growth of cells (Sf21 and Bm5) and viruses were examined on the various concentrations of FBS. In view of the viability, growth speed, proliferation of cells and the amount of FBS, the most proper concentration for the cell culture were 7% and 5% for Sf21 and Bm5, respectively. The multiplicity of viruses at the various concentrations of FBS was similar in both cell lines at 5 days post-infection (p.i.). However, it differed significantly at 2 and 3 days p.i. The proper concentration of FBS were 10% and 3% for Sf21 at 2 and 3 days p.i., respectively, and 5% for Bm5 at both 2 and 3 days p.i. These results suggested that the optimal concentration of FBS should be determined according to the used cell lines and viruses for their optimum production.

곤충 세포주 Sf21과 Bm5 세포주에 대해 세포 배양과 바이러스의 증식을 위한 최적 FBS 의 농도를 결정하기 위하여 다양한 FBS 농도에서 세포 및 바이러스의 증식 곡선을 비교하였다. 세포의 생존율, 증식속도, 증식량 그리고 FBS 함량을 모두 고려할 때 Sf21 에 대해서는 7%가, Bm5에 대해서는 5% FBS 가 최적 농도로 결정되었다. 바이러스의 증식은 감염 후 5일째에 두 세포주 모두 모든 FBS 농도에서 유사한 증식량을 보였으나, 감염 후 2 일과 3일에 있어서는 Sf21 은 각각 10%와 3%가 Bm5 에 대해서는 양일 모두 5% FBS 농도에서 가장 증식량이 높았다. 이러한 결과는 목적에 따라 세포 및 바이러스 증식을 위한 적정 FBS 농도의 결정이 필요함을 제시하는 것이다.

Keywords

References

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