• Journal of Soil and Groundwater Environment
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• v.21 no.5
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• pp.8-15
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• 2016

Bioremediation, which uses microbes to degrade most organic pollutants in soil and groundwater, can be used in solving environmental issues in various polluted sites. In this research, a wind-driven bioventing system is built to degrade about 20,000 mg/kg of high concentration diesel pollutants in soil-pollution mode. The wind-driven bioventing test was proceeded by the bioaugmentation method, and the indigenous microbes used were Bacillus cereus, Achromobacter xylosoxidans, and Pseudomonas putida. The phenomenon of two-stage diesel degradation of different rates was noted in the test. In order to interpret the results of the mode test, three microbes were used to degrade diesel pollutants of same high concentration in separated aerated batch-mixing vessels. The data derived thereof was input into the Haldane equation and calculated by non-linear regression analysis and trial-and-error methods to establish the kinetic parameters of these three microbes in bioventing diesel degradation. The results show that in the derivation of μ_m (maximum specific growth rate) in biodegradation kinetics parameters, K_s (half-saturation constant) for diesel substance affinity, and K_i (inhibition coefficient) for the adaptability of high concentration diesel degradation. The K_s is the lowest in the trend of the first stage degradation of Bacillus cereus in a high diesel concentration, whereas K_i is the highest, denoting that Bacillus cereus has the best adaptability in a high diesel concentration and is the most efficient in diesel substance affinity. All three microbes have a degradation rate of over 50% with regards to Pristane and Phytane, which are branched alkanes and the most important biological markers.

• The Korean Journal of Physiology and Pharmacology
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• v.9 no.1
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• pp.63-68
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• 2005

Hydrogen peroxide ($H_2O_2$) causes oxidative stress and is considered as an inducer of cell death in various tissues. Two-pore domain $K^+$ ($K_{2p}$) channels may mediate $K^+$ efflux during apoptotic volume decreases (AVD) in zygotes and in mouse embryos. In the present study, we sought to elucidate linkage between $K_{2p}$ channels and cell death by $H_2O_2$. Thus $K_{2p}$ channels (TASK-1, TASK-3, TREK-1, TREK-2) were stably transfected in HEK-293 cells, and cytotoxicity assay was preformed using cell counting kit-8 (CCK-8). Cell survival rates were calculated using the cytotoxicity assay data and dose-response curve was fitted to the $H_2O_2$ concentration. Ionic currents were recorded in cell-attached mode. The bath solution was the normal Ringer solution and the pipette solution was high $K^+$ solution. In HEK-293 cells expressing TREK-1, TREK-2, TASK-3, $H_2O_2$ induced cell death did not change in comparison to non-transfected HEK-293. In HEK-293 cells expressing TASK-1, however, dose-response curve was significantly shifted to the left. It means that $H_2O_2$ induced cell death was increased. In cell attached-mode recording, application of $H_2O_2$ (300μM) increased activity of all $K_{2p}$ channels. However, a low concentration of $H_2O_2$ ($50{\mu}M$) increased only TASK-1 channel activity. These results indicate that TASK-1 might participate in $K^+$ efflux by $H_2O_2$ at low concentration, thereby inducing AVD.

• Nuclear Engineering and Technology
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• v.24 no.1
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• pp.30-39
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• 1992

In this study, an integrated boration and dilution (INBAD) model is proposed to predict the required makeup flowrate for RCS boron concentration change and to analyze the boron concentration behavior at each subsystem within the RCS including CVCS during boration and dilution operation. The INBAD model is constructed by integrating an existing neutronic code and a boration and dilution model. The boration and dilution model has been developed for our specific purpose using the one-cell model and multi-cell model. In addition, in order to assess the boron concentration behavior more realistically, two important features such as variable pressurizer heater output and optional makeup mode (either direct or indirect injection) are implemented in this model. In order to demonstrate the usefulness of this model, the boron concentration behavior analysis at each subsystem were performed for both direct and indirect injection mode using YGN 3 and 4 design data. Also, the effect of pressurizer heater output on the primary loop boron concentration was investigated. The results showed that the boron concentration changes can be predicted accurately at each subsystem during boration and dilution operation.

• Journal of Positioning, Navigation, and Timing
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• v.6 no.4
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• pp.211-221
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• 2017

Ellipsodially referenced survey (ERS) is considered as one of the challenging issues in the hydrographic surveys due to the fact that the bathymetric data collected by this technique can be readily transformed either to the geodetic or the chart datum by application of some geoscientific models. Global Navigation Satellite Systems (GNSS) is a preferred technique to determine the ellipsoidal height of a vessel reference point (RP) because it provides cost-effective and unprecedentedly accurate positioning solutions. Especially, the GNSS-derived heights include heave and dynamic draft of a vessel, so as for the reduced bathymetric solutions to be potentially free from these corrections. Although over the last few decades, differential GNSS (DGNSS) has been widely adopted in the bathymetric surveys, it only provides limited accuracy of the vertical component. This technical barrier can be effectively overcome by adopting the so-called GNSS carrier phase (CPH) based techniques, enhancing accuracy of the height solution up to few centimeters. From the positioning algorithm standpoint, the CPH-based techniques are categorized under absolute and relative positioning in post-processing mode; the former is precise point positioning (PPP) correcting errors by the global or regional models, the latter is post-processed kinematic positioning (PPK) that uses the differencing technique to common error sources between two receivers. This study has focused on assessment of achievable accuracy of the ellipsoidal heights obtained from these CPH-based techniques with a view to their applications to hydrographic surveys where project area is, especially, few tens to hundreds kilometers away from the shore. Some field trials have been designed and performed so as to collect GNSS observables on static and kinematic mode. In this paper, details of these tests and processed results are presented and discussed.

• Steel and Composite Structures
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• v.16 no.1
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• pp.21-44
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• 2014

The aim of this study is to determine the dynamic characteristics of long reinforced concrete highway bridges with post-tension tendons using analytical and experimental methods. It is known that the deck length and height of bridges are affected the dynamic characteristics considerably. For this purpose, Berta Bridge constructed in deep valley, in Artvin, Turkey, is selected as an application. The Bridge has two piers with height of 109.245 m and 85.193 m, and the total length of deck is 340.0 m. Analytical and experimental studies are carried out on Berta Bridge which was built in accordance with the balanced cantilever method. Finite Element Method (FEM) and Operational Modal Analysis (OMA) which considers ambient vibration data were used in analytical and experimental studies, respectively. Finite element model of the bridge is created by using SAP2000 program to obtain analytical dynamic characteristics such as the natural frequencies and mode shapes. The ambient vibration tests are performed using Operational Modal Analysis under wind and human loads. Enhanced Frequency Domain Decomposition (EFDD) and Stochastic Subspace Identification (SSI) methods are used to obtain experimental dynamic characteristics like natural frequencies, mode shapes and damping ratios. At the end of the study, analytical and experimental dynamic characteristic are compared with each other and the finite element model of the bridge was updated considering the material properties and boundary conditions. It is emphasized that Operational Modal Analysis method based on the ambient vibrations can be used safely to determine the dynamic characteristics, to update the finite element models, and to monitor the structural health of long reinforced concrete highway bridges constructed with the balanced cantilever method.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.7
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• pp.712-718
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• 2008

This paper deals with a development of flight control law switching system which can be used for flight test of the research control law by switching control law during flight. Through this research program, fader logic and integrator stabilization design has been introduced to minimize the transient response of aircraft caused by flight control law switching and to prevent the divergence of the integrator included in the control law in standby mode. MIL-STD-1553B communication was applied to transfer the data between the two control laws. This paper introduce the control law switching system architecture and major design concept and include the system verification and validation result performed on the flying quality simulator of the advanced trainer.

• BMB Reports
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• v.31 no.2
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• pp.149-155
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• 1998

The N199H variant of the EcoRI endonuclease has about twice the catalytic activity of the wild-type. A comparison of their biochemical characteristics, using synthetic oligonucleotides 5'-dAAAACTTAAGAAAAAAAAAAA-3' (KA) and 5'-dTTTTTGAATTCTTTTTTTTTT-3' (KT), helps to define the cleavage reaction pathway of these enzymes. Both EcoRI and EcoRI variant N199H were found to cleave singlestranded KA or KT about three times faster than the double-stranded forms, although the KT oligonucleotide was more susceptible. Using the ssDNA substrate in kinetic analyses, lower $K_m$ values were obtained for the N199H variant than for the wild-type at low (50 mM), as well as high (200 mM), sodium chloride concentrations. This difference between the endonucleases is attributed to a grealter accessibility for tbe substrate by the variant, and also a higher affinity for the DNA backbone. It also appears that the relative activities of the two enzymes, particularly at high ionic strength, are proportional to their populations in the monomeric enzyme form. That is, according to gel filtration data, half of the N199H molecules exist as monomers in 200 mM NaCl, whereas those of the wild-type are mainly dimeric. Consequently, the Asp199 residue of the EcoRI endonuclease may be implicated in the protein-protein interaction leading to dimerization, as well as in coupling to DNA substrates. In summary, it is proposed that active monomeric endonuclease molecules, derived from the dimeric enzyme, recognize and form a complex with a single stranded form of the DNA substrate, which then undergoes nucleophilic substitution and cleavage.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.5 no.2
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• pp.131-140
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• 2000

Heavy metals (Fe, Mn, Cr, Co, Cu, Ni, Zn, Pb) were determined in ninety-one surface sediments collected from Kwangyang Bay, south coast of Korea. The data show that the Fe, Cr, Ni, and Zn distribution in the bay can be described by metal vs. grain size relationship. However, the distribution pattern of Mn, Co, Cu and Pb were found to be changed because they are anthropogenically enriched to sediment. Correlation matrix and R-mode factor analyses revealed that two important factors controlling the distribution of metals in the bay are grain size and anthropogenic input.

• Journal of Hydrogen and New Energy
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• v.19 no.5
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• pp.403-409
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• 2008

Polymer electrolyte membrane fuel cell (PEMFC) is very interesting power source due to high power density, simple construction and operation at low temperature. But it has problems such as high cost, improvement of performance, effect of temperature and initial start at low temperature. These problems can be approached to be solved by using experiment and mathematical method which are general principles for analysis and optimization of control system for heat and hydrogen detecting management. In this paper, insulation vessel and control system for stable operation of fuel cell at low temperature were developed for experiment. The constant temperature capability and the heating time at sub-zero temperatures with insulation control system were studied by using a heating bar of 60W class. PEMFC stack which was made by 4 cells with $50\;mc^2$ active area in each cell is a thermal source. Times which take to reach constant temperature by the state of insulation vacuum were measured at variable environment temperatures. The test was performed at two conditions: heating mode and cooling mode. Constant temperature capability was better at lower environment temperature and vacuum pressure. The results of this experiment could be used as basis data about stable operation of fuel cell stack in low temperature zone.

• The Journal of Advanced Prosthodontics
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• v.4 no.3
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• pp.139-145
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• 2012

PURPOSE. To evaluate the shear bond strength of resin luting agent to dentin surfaces cleansed with different agents like pumice, ultrasonic scaler with chlorhexidine gluconate, EDTA and the influence of these cleansing methods on wetting properties of the dentin by Axisymmetric drop Shape Analysis - Contact Diameter technique (ADSA-CD). MATERIALS AND METHODS. Forty coronal portions of human third molar were prepared until dentin was exposed. Specimens were divided into two groups: Group A and Group B. Provisional restorations made with autopolymerizing resin were luted to dentin surface with zinc oxide eugenol in Group A and with freegenol cement in Group B. All specimens were stored in distilled water at room temperature for 24 hrs and provisional cements were mechanically removed with explorer and rinsed with water and cleansed using various methods (Control-air-water spray, Pumice prophylaxis, Ultrasonic scaler with 0.2% Chlorhexidine gluconate, 17% EDTA). Contact angle measurements were performed to assess wettability of various cleansing agents using the ADSA-CD technique. Bond strength of a resin luting agent bonded to the cleansed surface was assessed using Instron testing machine and the mode of failure noted. SEM was done to assess the surface cleanliness. Data were statistically analyzed by one-way analysis of variance with Tukey HSD tests (${\alpha}$=.05). RESULTS. Specimens treated with EDTA showed the highest shear bond strength and the lowest contact angle for both groups. SEM showed that EDTA was the most effective solution to remove the smear layer. Also, mode of failure seen was predominantly cohesive for both EDTA and pumice prophylaxis. CONCLUSION. EDTA was the most effective dentin cleansing agent among the compared groups.