• Title/Summary/Keyword: Tube-by-tube method

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Comparison among the Sperm Preparation Methods on the Human Spermatozoa (인간정자에 있어서 정자처리법의 비교)

  • Pang, Myung-Geol;Chung, Ku-Min;Shin, Chang-Jae;Kim, Jung-Gu;Moon, Shin-Yong;Chang, Yoon-Seok;Lee, Jin-Yong;Lee, Sang-Hoon;Chung, Yung-Chai;Kim, Chang-Keun
    • Clinical and Experimental Reproductive Medicine
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    • v.20 no.2
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    • pp.107-115
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    • 1993
  • Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.

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Image Based Quality Assurance of Range Compensator for Proton Beam Therapy (양성자치료용 보상체의 영상기반 정도 관리 기반 프로그램 개발)

  • Kim, Jin-Sung;Yoon, Myong-Geun;Kim, Dong-Wook;Lim, Young-Kyung;Kwak, Jung-Won;Park, So-Ah;Shin, Dong-Ho;Shin, Jung-Wook;Lee, Se-Byeong;Park, Sung-Yong;Cho, Kwan-Ho
    • Progress in Medical Physics
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    • v.19 no.1
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    • pp.35-41
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    • 2008
  • The main benefit of proton therapy over photon beam radiotherapy is the absence of exit dose, which offers the opportunity for highly conformal dose distributions to target volume while simultaneously irradiating less normal tissue. For proton beam therapy two patient specific beam modifying devices are used. The aperture is used to shape the transverse extension of the proton beam to the shape of the tumor target and a patient-specific compensator attached to the block aperture when required and used to modify the beam range as required by the treatment plan for the patient. A block of range shifting material, shaped on one face in such a way that the distal end of the proton field in the patient takes the shape of the distal end of the target volume. The mechanical quality assurance of range compensator is an essential procedure to confirm the 3 dimensional patient-specific dose distributions. We proposed a new quality assurance method for range compensator based on image processing using X-ray tube of proton therapy treatment room. The depth information, boundaries of each depth of plan compensatorfile and x-ray image of compensator were analyzed and presented over 80% matching results with proposed QA program.

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Structural Reinforcement Methods and Structural Safety Analysis for the Elevated Eaves Height 1-2W Type Plastic Greenhouse (측고를 높인 1-2W형 비닐하우스의 구조안전성 분석 및 구조보강 방법)

  • Ryu, Hee-Ryong;Yu, In-Ho;Cho, Myeong-Whan;Um, Yeong-Cheol
    • Journal of Bio-Environment Control
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    • v.18 no.3
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    • pp.192-199
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    • 2009
  • This study was conducted to find proper structural reinforcement methods for the 4.5m-high (eaves height) 1-2W type plastic greenhouse. 3D finite element analysis was used to analyze the steel-tube structure. The 4.5m-high 1-2W type plastic greenhouse was modified by welding 1.5m-long steel-pipes into a 3.0m-tall columns of the standard 1-2W type plastic greenhouse. This remodeling method is widely used in Korea with farmer's discretion to increase the production when they grow paprika. But it is not based on the quantitative structural analysis. The proposed reinforcement methods were proved to stand against the design wind velocity of $40m{\cdot}s^{-1}$ and snow depth of 40cm. It strongly implies that the cross beam between side columns and wind resistance walls, and the lattice type cross beam should be good reinforcements to improve the structural safety of the elevated eaves height plastic greenhouse.

Quantitative Analysis of Bacillus amyloliquefaciens GR4-5 in Soil (Bacillus amyloliquefaciens GR4-5 균주의 토양 내 정량 분석)

  • Kim, Dayeon;Kim, Byung-Yong;Ahn, Jae-Hyung;Weon, Hang-Yeon;Kim, Sung-Il;Kim, Wan-Gyu;Song, Jaekyeong
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.847-858
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    • 2015
  • Bacillus amyloliquefaciens GR4-5 was isolated from the rhizosphere soil of Korean ginseng and displayed broad-spectrum suppression of ginseng root rot pathogens. The survivability of B. amyloliquefaciens GR4-5 in soil was investigated under three different conditions; indoor, outdoor - of which soil was put in 14 mL tube after treatment - and field environments. Soil samples were collected over a four-week period from three experimental designs, and assessed for 16S rRNA gene copy number by quantitative polymerase chain reaction (qPCR). In outdoor condition, the 16S rRNA gene copy number of Bacillus spp. was 8.35 log copies g $soil^{-1}$ immediately after the GR4-5 treatment. Two weeks later, the 16S rRNA gene copy number of Bacillus spp. (6.70 log copies g $soil^{-1}$) was similar to that of the control (6.38 log copies g $soil^{-1}$). In indoor condition, the 16S rRNA gene copy number of Bacillus spp. maintained in a certain level for a longer period than those in outdoor and field. The 16S rRNA gene copy number of Bacillus spp. in field experiment was reduced faster than that of outdoor condition. Our results show that B. amyloliquefaciens GR4-5 can survive in bulk soil for 1 week, indicating its potential use as a biocontrol agent following 7 day application intervals. This study presents that outdoor microcosm system design could be a useful method to assess easily the survivability of beneficial microorganisms.

Exposure Characteristics of 1,3-Butadiene Exposed Workers (1,3-부타디엔 제조 및 취급 근로자의 노출특성에 관한 연구)

  • Choi, Ho Chun;An, Sun Hee;Lee, Hyun Seok;Park, Young Wook;Kim, Kyung Soon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.19 no.4
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    • pp.321-327
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    • 2009
  • 1,3-butadiene is classified as suspected human carcinogen, group A2(American Conference of Governmental Industrial Hygienists, ACGIH). In Korea, 1,3-butadiene has been used as a raw material; monomer, homopolymer, polybutadiene latex, acrylonitrile-butadiene-styrene(ABS) and styrene-butadiene rubber(SBR), in the petrochemistry and precision chemistry industry. As petrochemistry industry in Korea has been developed, the potential exposure possibility of 1,3-butadiene to workers can be increased. Therefore the purpose of this study is to evaluate airborne 1,3-butadiene concentration and workers' exposure levels in the workplace using 1,3-butadiene. Air samples were collected with 4-tert-butyl catechol(TBC) charcoal tube(100 mg/50 mg) and were analyzed by gas chromatograph/flame ionization detector(GC/FID) according to the Choi's method(2002). Geometric mean (GM) and arithmetic mean (AM) of total 59 workers' exposure concentrations to airborne 1.3-butadiene were 0.042 ppm and 1.51 ppm, respectively. Although most samples were lower than 1ppm, 2 samples(21.5ppm and 33.1ppm as 8hr-TWA) were exceeded the Korean standard(2ppm) over 10 times at the repair process in synthetic rubber and resin manufacture industry. 14 samples(41%) of total 34 short-term air samples were exceeded the Korean standard(10ppm as STEL) of Ministry Labor. 1,3-butadiene concentration(GM) in the synthetic rubber and resin manufacture industry(7.87ppm) was significantly higher than that in the monomer manufacure industry (0.35ppm)(p<0.05). Also in the sampling and repair process, each GM(range) was 1.39ppm(N.D.-469.6ppm) and 7.85ppm(N.D.-410.2ppm). In conclusion, it depends on the industry and process, 1,3-butadiene can be exposed to workers as high concentration for short-term.

Suggestion on Strength Formula of Square Hollow Section Tubluar Column-to-BeamPinned Connections (각형강관 기둥-보 핀접합부의 내력식 제안)

  • Choi, Sung Mo;Lee, Seong Hui;Lee, Kwang Ho
    • Journal of Korean Society of Steel Construction
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    • v.18 no.5
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    • pp.525-534
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    • 2006
  • Column-to-beam pinned connections can cause local moment to the web of a steel tube due to the distance of eccentricity between the row of bolts and the column flange, which possibility deteriorates the load capacity of column. In this study, a square hollow section tubular used finite element analysis of a square hollow section tubular column was carried out, and the column width and thickness, existence and non-existence of internal reinforcement, and existence and non-existence of compressive force were taken as variables to examine the load capacity deterioration of a square column caused by moment. To guarantee the reliability of the finite element results, some specimens were fabricated and tested. The yield line method was applied to suggest the strength formulas of the square tubular column to the beam pinned connections. Based on the study results, the column strength the moment of the square hollow section tubular column to the beam pined connections improved with the increase in the w to strength limitations, a no-reinforcement type of square hollow section tubular column was proposed, and if the limitation values were not satisfied, the reinforcement of the internal column was made mandatory. Therefore, the horizontal -reinforcement type considered the strength increase, and the fabrication of the square hollow section tubular column was ar column that considered its load capacity with the moment for the no-reinforcement and the horizontal-reinforcement types.

Design of Sensor Network for Estimation of the Shape of Flexible Endoscope (연성 대장내시경의 형상추정을 위한 센서네트워크의 설계)

  • Lee, Jae-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.2
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    • pp.299-306
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    • 2016
  • In this paper, a method of shape prediction of an endoscope handling robot that can imitate a surgeon's behavior using a sensor network is suggested. Unit sensors, which are composed of a 3-axis magnetometer and 3-axis accelerometer pair comprise the network through CAN bus communication. Each unit of the sensor is used to detect the angle of the points in the longitudinal direction of the robot, which is made from a flexible tube. The signals received from the sensor network were filtered using a low pass Butterworth filter. Here, a Butterworth filter was designed for noise removal. Finally, the Euler angles were extracted from the signals, in which the noise was filtered by the low path Butterworth filter. Using this Euler angle, the position of each sensor on the sensor network is estimated. The robot body was assumed to consist of links and joints. The position of each sensor can be assumed to be attached to the center of each link. The position of each link was determined using the Euler angle and kinematics equation. The interpolation was carried out between the positions of the sensors to be able to connect each point smoothly and obtain the final posture of the endoscope in operation. The experimental results showed that the shape of the colonoscope can be visualized using the Euler angles evaluated from the sensor network suggested and the shape of serial link estimated from the kinematics chain model.

Development of the Small Scale Testbed for Running Dynamic Characteristics Analysis of the Capsule Train (캡슐트레인 주행 동특성 분석을 위한 축소 시험장치의 개발)

  • Lee, Jin-Ho;You, Won-Hee;Lee, Kwansup
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.9
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    • pp.643-651
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    • 2020
  • A capsule train runs inside a sub-vacuum tube and can reach very high speed due to the low air resistance. A capsule train uses a superconducting electrodynamic suspension (SC-EDS) method for levitation, which allows for a large levitation gap and does not require gap control. However, SC-EDS has inherent characteristics such as the large gap variation and a small damping effect in the levitation force, which can degrade the running stability and ride comfort. To overcome this, a stability improvement device should be designed and applied based on dynamic analysis. In this study, a 1/10 small-scale testbed was developed to replicate the dynamic characteristics of a capsule train and investigate the performance of stability improvement devices. The testbed is composed of a 6-degree-of-freedom Stewart platform for the realization of bogie motion, a secondary suspension with a running stabilization device, and a carbody. Based on the dynamic similarity law proposed by Jaschinski, the small-scale testbed was manufactured, and a bogie motion algorithm was applied with the consideration of guideway irregularity and levitation stiffness. The experimental results from the testbed were compared with simulation results to investigate the performance of the testbed.

A Study on the Distribution of Scatter Ray in Chest Radiography of a Health Examination Bus (건강검진 차량 내 흉부 방사선검사 시 공간산란선 분포 연구)

  • Cho, Ji-Hwan;Jin, Seong-jin;Min, Byeong-In
    • Journal of radiological science and technology
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    • v.40 no.3
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    • pp.377-383
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    • 2017
  • The purpose of this study was to evaluate the distribution of spatial scatter ray on the chest radiographs of patients on health examination bus. In this paper, we propose a method for minimize unnecessary exposure by measuring the scattered dose after exposure the actual subject and comparing the body mass index (BMI) with the tube current amount mAs. The results of this study showed that the mean BMI of the subjects was $23.31{\pm}3.12$. The mean mAs value was $2.92{\pm}1.19$, which males was higher than females. The mean value of the scatter ray at position 1 in the radiography room was $771.81{\pm}151.15{\mu}Sv/hr$. The mean value of the scatter rays at the position 2 outside the entrance of the radiography room was measured as $53.86{\pm}25.66{\mu}Sv/hr$. As the BMI and mAs was increase the spatial scatter dose was increased at position 1 and position 2 in the photographing room. In order to minimize the exposure dose of scatter ray, radiation workers should shoot the radiation as low as possible within the range that does not impair the quality of the image. It will be necessary to make efforts to not wait for a waiting person near the entrance door of the photographing room.

Analysis of Photon Characteristics and Absorbed Dose with Cone Beam Computed Tomography (CBCT) using Monte Carlo Method (몬테칼로 기법을 이용한 CBCT의 광자선 특성 및 선량 분석)

  • Kim, Jong-Bo;Kim, Jung-Hoon;Park, Eun-Tae
    • Journal of the Korean Society of Radiology
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    • v.11 no.3
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    • pp.161-169
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    • 2017
  • The cone beam computed tomography(CBCT) which can acquire 3-dimensions images is widely used for confirmation of patient position before radiation therapy. In this study, through the simulation using the Monte Carlo technique, we will analyze the exposure dose by cone beam computed tomography and present the standardized data. For the experiment, MCNPX(ver. 2.5.0) was used and the photon beam spectrum was analyzed after Cone beam was simulated. As a result of analyzing the photon beam spectrum, the average energy ranged from 25.7 to 37.6 keV at the tube voltage of 80 ~ 120 kVp and the characteristic X-ray energy was 9, 60, 68 and 70 keV. As a result of using the water phantom, the percentage depth dose was measured, and the maximum dose appeared on the surface and decreased with depth. The absorbed dose also decreased as the depth increased. The absorbed dose of the whole phantom was 9.7 ~ 18.7 mGy. This is a dose which accounts for 0.2% of about 10 Gy, which is generally used for radiation therapy per week, which is not expected to have a significant effect on the treatment effect. However, it should not be overlooked even if it is small compared with prescription dose.