• Title/Summary/Keyword: Tube-by-tube method

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Culture Tube Method for the Determination of Total Cholesterol in Egg Yolk Lipid (Culture Tube 방법에 의한 난황중의 Cholesterol 정량)

  • Ha, Yeong-Lae;Kim, Jeong-Ok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.6
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    • pp.1032-1037
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    • 1994
  • A simple, reproducible , and accurate enzymatic method using a cholesterol assay kit was developed to quantify total cholesterol content in egg yolk. Total egg yolk lipid was extracted with hexane : isopropanol(3 : 2, v/v) mixture. Samples containing various amount of the total lipid(0-3mg) in optically identifical culture tubes were reacted for 10 min in a water bath (37$^{\circ}C$) with the enzyme solution (5ml) from the cholesterol assay kit. Cholesterol content of the reaction mixturesin culture tubes was spectrophotometrically determined by two different ways : (1) using the culture tube as a curvette(designate culture tube method ; CTM) and (2) the quartz cvette containing the reaction mixture transferred from the culture tube (designate standard cvette method, SCM). CTM revealed lower cholesterol content in 0.1-1.0mg lipid sample range that SCM did, but not significant. For more than 2.0mg lipid sample, CTM gave significantly (p<0.01) lower cholesterol content relative to that by SCM, suggesting that SCM give a false positive result from the sample containing more than 2 mg lipid due to the interference of absorbance by lipid dispersed in the reaction solution . Cholesterol content of less than 1.0mg lipid sample by CTM was proportional to the amount of lipid used, but its linear relationship was not seen in more than 2mg lipid sample. Thus, to determine the appropriate lipid amounts (mg) analyzed . A constant level (41$\mu\textrm{g}$/mg) of cholesterol concentration was observed from the sample containing 0.1-1mg lipid. after which the cholesterol level was dropped to less than 41$\mu\textrm{g}$ /mg. Cholesterol concentration in egg yolk samples quantified by CTM was in accordance with that by GC method. These results suggest that CTM is an useful method for the quantification of cholesterol in egg yolk lipid and other lipids as well.

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Ventilation Load Reduction Plan Using Cool Tube System Case (Cool Tube System 사례를 활용한 환기부하 절감방안)

  • Jeong, Min Yeong;Park, Jin Chul;Yang, Young Kwon
    • Land and Housing Review
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    • v.10 no.1
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    • pp.25-32
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    • 2019
  • In this study, the case analysis data on underground temperature are presented. In addition, numerical analysis of the ventilation load reduction plan was derived according to the residence schedule change for the building with cool tube. The research scope and method are as follows. The overall system principle was examined through reviewing the theory of the Cool tube system. Case study and analysis were conducted. Numerical simulation was used to examine the change in energy usage. Also, the change of load energy in case of varying amount of ventilation was derived based on actual building room schedule. When the Cool tube system was applied to the residential buildings, the cooling load was reduced from 3,331 kW to 193 kW, which showed a reduction effect of about 90%.The heating load was reduced from 42,276kW to 32,575kW by 23%.Also, result shows that the cooling load decreased by 24% and the heating load decreased by 66% when the number of ventilation according to the occupancy schedule was applied.

Ultrasonic Measurement of Gap between Calandria Tube and Liquid Injection Nozzle in CANDU Reactor (초음파를 이용한 중수로내 칼란드리아관과 원자로 정지물질 주입관과의 간격 측정)

  • Sohn, Seok-Man;Kim, Tae-Rong;Lee, Jun-Sin;Lee, Young-Hee;Park, Chul-Hun
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.834-839
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    • 2001
  • Calandria tube wrapping each pressure tube is one of the key structural components of CANDU reactor(Calandria) which is consisted of many pressure tubes containing nuclear fuel assemblies. As the Calandria tube(made of zirconium alloy) is sagging due to its thermal and irradiation creep during the plant operation, it possibly contacts with liquid injection nozzle crossing beneath the Calandria tube, which subsequently results in difficulties on the safe operation. It is therefore necessary to check the gap for the confirmation of no contacts between the two tubes, Calandria tube and liquid injection tube, with a proper measure during the life of plant. In this study, an ultrasonic measurement method was selected among several methods investigated. The ultrasonic device being developed for the measurement of the gap was introduced and its preliminary performance test results were presented here. The gap between LIN and CT at site was measured using by this ultrasonic device at site.

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Study of the Periodic Ludwieg Tube Flow with Heat Addition (가열을 수반하는 Ludwieg Tube 유동에 대한 연구)

  • Baek, S.C.;Kwon, S.B.;Kim, H.D.
    • Proceedings of the KSME Conference
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    • 2001.11b
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    • pp.450-455
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    • 2001
  • The time-dependent behavior of nonequilibrium condensation of moist air through the Ludwieg tube is investigated with a computational fluid dynamics(CFD) method. The two-dimensional, compressible, Navier-Stokes equations, fully coupled with the condensate droplet growth equations, are numerically solved by a third-order MUSCL type TVD finite-difference scheme, with a second-order fractional time step. Baldwin-Lomax turbulence model is employed to close the governing equations. The computational results are compared with the previous experiments using the Ludwieg tube with a downstream diaphragm. The results clearly show that for an initial relative humidity below 30% there is no periodic oscillation of the condensation shock wave, but for an initial relative humidity over 40% the periodic excursions of the condensation shock occurs in the Ludwieg tube, and the frequency increases with the initial relative humidity. It is also found that total pressure loss due to nonequilibrium condensation in the Ludwieg tube should not be ignored even for a very low initial relative humidity, and the periodic excursions of the condensation shock wave are responsible for the total pressure loss.

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A Study on the Heat Transfer Improvement in the Exhaust-Section of Industrial Furnace (산업용로 배기부에서의 열전달 향상에 관한 연구)

  • Pak, Hi-Yong;Cha, Sang-Myung;Han, Chul-Hee
    • Solar Energy
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    • v.12 no.3
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    • pp.47-59
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    • 1992
  • In the concentric tube type recuperator, which is the most typical type of radiation recuperator, installed on the exhaust-secion of industrial furnace, air flows between the adiabatic outer tube and the inner tube in which exhausted gas flows with high temperature. The waste heat of the exhausted gas is transferred to the inner tube, and transferred from the inner tube to the flowing air. The heat transfer by radiation In the concentric tube type recuperator is modeled using spherical harmonics approximation, namely, P-N method and numerically analyzed considering the effect of dynamic flow field. The results are compared with the existing empirical data. In addition, a theoretical method is presented for the analysis of the heat transfer characteristics of a recuperator with a reradiator installed in the inner tube, which causes re-radiant in the inner tube, and the characteristics of the recuperator is analyszed and defined.

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MATERIAL RELIABILITY OF Ni ALLOY ELECTRODEPOSITION FOR STEAM GENERATOR TUBE REPAIR

  • Kim, Dong-Jin;Kim, Myong-Jin;Kim, Joung-Soo;Kim, Hong-Pyo
    • Nuclear Engineering and Technology
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    • v.39 no.3
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    • pp.231-236
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    • 2007
  • Due to the occasional occurrences of stress corrosion cracking(SCC) in steam generator tubing(Alloy 600), degraded tubes are removed from service by plugging or are repaired for re-use. Since electrodeposition inside a tube does not entail parent tube deformation, residual stress in the tube can be minimized. In this work, tube restoration via electrodeposition inside a steam generator tubing was performed after developing the following: an anode probe to be installed inside a tube, a degreasing condition to remove dirt and grease, an activation condition for surface oxide elimination, a tightly adhered strike layer forming condition between the electro forming layer and the Alloy 600 tube, and the condition for an electroforming layer. The reliability of the electrodeposited material, with a variation of material properties, was evaluated as a function of the electrodeposit position in the vertical direction of a tube using the developed anode. It has been noted that the variation of the material properties along the electrodeposit length was acceptable in a process margin. To improve the reliability of a material property, the causes of the variation occurrence were presumed, and an attempt to minimize the variation has been made. A Ni alloy electrodeposition process is suggested as a primary water stress corrosion cracking(PWSCC) mitigation method for various components, including steam generator tubes. The Ni alloy electrodeposit formed inside a tube by using the installed assembly shows proper material properties as well as an excellent SCC resistance.

Field Application and Performance of Continuously Reinforced Concrete Pavement via Mechanical Tube-feeding Method (기계식 연속철근콘크리트포장의 현장 적용성 및 거동 분석 연구)

  • Choi, hooseok
    • International Journal of Highway Engineering
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    • v.18 no.2
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    • pp.43-49
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    • 2016
  • PURPOSES : The field application and performance of continuously reinforced concrete pavement (CRCP), constructed by using the mechanical tube-feeding method, are evaluated in this study. METHODS: The location of the rebar was evaluated by using the MIRA system. The early-age CRCP performance was evaluated via visual survey, in which the crack spacing and crack width were examined. RESULTS: The location of longitudinal reinforcing bars was evaluated via MIRA testing and the results showed that the longitudinal rebars all lie within a given tolerance limit (${\pm}2.5cm$) of the target elevation. In addition, owing to the low temperature when the concrete was pured, the crack spacing in the Dae-Gu direction is slightly wider than that of the Gwang-Ju direction. Almost all of the crack spacings lay within the range of 1.0 m~3.0 m. A crack width of <0.3 mm was measured at the pavement surface. However, as revealed by the field survey, the crack spacing was not correlated with the crack width. CONCLUSIONS : In CRCP constructed by using the mechanical tube-feeding method, almost all of the longitudinal reinforcing bars lay within the tolerance limit (2.5 cm) of the target elevation. The concrete-placing temperature affects the crack spacing, owing to variations in the zero-stress temperature. Crack survey results show that there is no correlation between the crack spacing and crack width in CRCP.

Analytical Study on Ultimate Design Method of Tube Flange Joints with the Rib Plate Using the High Strength Bolt (리브 붙은 고력볼트 강관플랜지 이음의 극한 설계방법에 관한 해석적 연구)

  • Kim, Seong Eun
    • Journal of Korean Society of Steel Construction
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    • v.12 no.5 s.48
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    • pp.537-547
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    • 2000
  • The tube flange joint often used in the field is a kind of tensional joint method using the high strength bolts. Transferring stress is conducted by high axial pressure between each part of material that is produced by twisting the high strength bolts. And historical characteristics of the flange joint have not been studied sufficiently and it is difficult to say that the design method is established definitely. Therefore new method using ultimate strength is need to be suggested to solve there problems in using flange joint. The purposes in this study are to gain the data base for establishing design method of joint in the form like figure1 and survey whether the joint of tube flange with non-equal diameter can be designed or not in the form like rib or ring.

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Non-Destructive Detection of Hydride Blister in PHWR Pressure Tube Using an Ultrasonic Velocity Ratio Method

  • Cheong Yong-Moo;Lee Dong-Hoon;Kim Sang-Jae;Kim Young-Suk
    • Nuclear Engineering and Technology
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    • v.35 no.5
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    • pp.369-377
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    • 2003
  • Since Zr-2.5Nb pressure tubes have a high risk for the formation of blisters during their operation in pressurized heavy water reactors, there has been a strong incentive to develop a method for the non-destructive detection of blisters grown on the tube surfaces. However, because there is little mismatch in acoustic impedance between the hydride blisters and zirconium matrix, it is not easy to distinguish the boundary between the blister and zirconium matrix with conventional ultrasonic methods. This study has focused on the development of a special ultrasonic method, so called ultrasonic velocity ratio method for a reliable detection of blisters formed on Zr-2.5Nb pressure tubes. Hydride blisters were grown on the outer surface of the Zr-2.5Nb pressure tube using a cold finger attached to a steady state thermal diffusion equipment. To maximize a difference in the ultrasonic velocity in hydride blisters and the zirconium matrix, the ultrasonic velocity ratio of longitudinal wave to shear wave, $V_L/V_S$, has been determined based on the flight time of the longitudinal echo and reflected shear echo from the outer surface of the tubes. The feasibility of the ultrasonic velocity ratio method is confirmed by comparing the contour plots reproduced by this method with those of the blisters grown on the Zr-2.5Nb pressure tubes.

A Study on Tube Feeding Practices of Adult In-patients (병원 성인 환자의 경관급식에 관한 연구)

  • 한경희
    • Journal of Nutrition and Health
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    • v.25 no.7
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    • pp.668-683
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    • 1992
  • To evaluate the current practices of the tube feeding and the status of tube feeding patient 76 adult in-patients at 6 hospitals located in Seoul and Chung-buk province were examined through reviewing patient charts observing patients and interviewing patients nurses dietitians patients' family or care-givers. The results were as follows : 1) An average age of the patients was 54.5 years with 41% over 60 years old. Patients with decreased mental status dysphagia esophageal obstruction and respiratory problem were fed by tubes. 2) The range of duration of tube feeding is between 4 days and 6 years. Most patients were received formula through nasogastric tube(89.5%) while 7.9% of gastrostomy and 2.6% of jejunostomy. Administration method for formula were bolus feeding regardless of the route of formula delivery. 3) Mean total calories received for men were 1590 kcal and 1450 kcal for female. Mean volume per meal was 282m, l and mean frequency of feeding was 5.68 while mean feeding interval 3$\frac{1}{4}$ hours and mean rate of infusion 68.4ml/min. All patients received hospital-blenderi-zed formula as the major source of nutrition. Home-blenderized formula and commercial formula as a supplement were used 35%, 13.2% respectively. 4) Thirty-eight percent of patients was hypoalbuminemia and 61% was at the moderate level of deficiency in hemoglobin. 5) Complications associated with tube feeding were diarrhea (22.4%) constipation(21.1%) vomiting(11.8%) and so on. 6) Serum albumin levels of patients who have complications associated with tube feeding were significantly lower than those of patients without complications In planning a tube feeding regimen the type of a formula must be integrated with both a delivery system and a protocol for administering the tube feeding. the multidisciplinary effort required to deliver enteral therapy is essential to improve current practices used at hospitals.

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