• 제목/요약/키워드: Trnasfection

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Induction of Tyrosine Hydroxylase by Nurr-1 in hES Cells

  • An So-Yeon;Lee Yeong-Jae;Kim Eun-Yeong;Jo Hyeon-Jeong;Choe Gyeong-Hui;Park Se-Pil;Im Jin-Ho
    • 한국동물번식학회:학술대회논문집
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    • 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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    • pp.85-85
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    • 2002
  • As an effort to direct differentiation of human embryonic stem cells (hES, MB03) to dopamine-producing neuronal cells, we expressed Nurr-l in hES and examined the expression of tyrosine hydroxylase (TH) after bFGF induction. To introduce Nurr-l, hES cells were maintained in humidified chamber with 5% CO₂ and 95% air in DMEM/Fl2 supplemented with FBS (10%), penicillin (100U/㎖), and streptomycin (100㎍/㎖). (omitted)

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