Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2002.06a
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- Pages.85-85
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- 2002
Induction of Tyrosine Hydroxylase by Nurr-1 in hES Cells
- An So-Yeon ;
- Lee Yeong-Jae ;
- Kim Eun-Yeong ;
- Jo Hyeon-Jeong ;
- Choe Gyeong-Hui ;
- Park Se-Pil ;
- Im Jin-Ho
- Published : 2002.06.01
Abstract
As an effort to direct differentiation of human embryonic stem cells (hES, MB03) to dopamine-producing neuronal cells, we expressed Nurr-l in hES and examined the expression of tyrosine hydroxylase (TH) after bFGF induction. To introduce Nurr-l, hES cells were maintained in humidified chamber with 5% CO₂ and 95% air in DMEM/Fl2 supplemented with FBS (10%), penicillin (100U/㎖), and streptomycin (100㎍/㎖). (omitted)