• Korean Journal of Microbiology
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• v.44 no.4
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• pp.339-345
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• 2008

This study was to detect MBL (metallo-${\beta}$-lactamase) among glucose non-fermenting Gram-negative bacilli isolated from clinical specimen and to search antimicrobial combination therapy against MBL producing Pseudomonas aeruginosa. Among fifty one isolates of Gram-negative bacilli with reduced imipenem susceptibility ($MIC{\ge}8{\mu}g/ml$), nine isolates have shown positive results in MBL detection test. They were seven Achromobacter xylosoxidans subsp. xylosoxidans and two P. aeruginosa. The results from EDTA-DDST coin-cided with those of PCR and nucleotide sequence analysis which showed the presence of $bla_{VIM-2}$. The combination of aztreonam (AZT) and piperacillin-tazobactarn (TZP) or AZT and amikacin (AN) screened by one disk synergy test showed no synergistic effect. Triple antibiotic combination therapy with AZT, TZP and AN, however, was shown to be effective and the most synergistic after 8 hrs of exposure. This result strongly suggest that the triple combination therapy of AZT, TZP, and AN could be useful for the treatment of infection caused by MBL producing Gram-negative bacilli.

• Journal of Convergence for Information Technology
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• v.11 no.9
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• pp.173-181
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• 2021

The purpose of this study is to prove the existence of the meridians by analyzing the effect of bloodletting cupping on Back-Shu Points and venesection on sabonghyeul, which are treatments for gastrointestinal and circulatory diseases with Ryodoraku. We selected the charts of 30 patients who received the treatment such as bloodletting cupping on Back-Shu Points and venesection on sabonghyeul for gastrointestinal and circulatory diseases. First, the Ryodoraku test was conducted in a stable state. After taking a rest on the bed, he performed a venesection on sabonghyeul. And bloodletting cupping on Back-Shu Points was performed with prone position. After all the procedures were completed, Ryodoraku test was performed again. And the result was compared with previous test status. By bloodletting cupping on Back-Shu Points and venesection on sabonghyeul, the deviation between the current value of the right F5 (gallbladder)· H4 (small intestine)· H6 (large intestine) (p<.05, p<.05, p<.001), left H5 (triple energizer) (p<.05) and the average current of 24 source points was significantly decreased. The difference between the left and right currents at the site of F3 (kidney) showed a significant increase (p<.005). It can be proved the existence of meridians that Bloodletting cupping on Back-Shu Points and venesection on sabonghyeul showed significant changes in the F5 (gallbladder), H4 (small intestine), H6 (large intestine), F3 (kidney), H5 (triple energizer) in digestive and circulatory symptoms by Ryodoraku test.

• Journal of Technologic Dentistry
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• v.41 no.2
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• pp.63-69
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• 2019

Purpose: The purpose of this study is to evaluate the effect of CAD/CAM system milling tool wear on the marginal and internal fit of PMMA implant interim prosthesis three-dimensional manner. Methods: A total of 20 crowns were fabricated with CAD/CAM method. Their designs were unified to first molar of the left maxilla. The Customized abutments were prepared and scanned with on optical model scanner. Five crowns were milled by the newly replaced tool (1st milling), and 15 crowns were milled by 2nd, 3rd, 4th milling tool. The marginal and internal fit of 20 interim crowns were measured using the triple-scan protocol. Results: Statistically significant difference was found between the $1^{st}$ milling group ($51.8{\pm}14.6{\mu}m$) and the $3^{rd}$, $4^{th}$ milling group ($128.6{\pm}43.8{\mu}m$, $146.2{\pm}38.1{\mu}m$, respectively) at the distal margins. In the mesial margins, There was a statistically significant difference between the $1^{st}$ milling group ($63.6{\pm}25.9{\mu}m$) and the $3^{rd}$, $4^{th}$ milling group ($137.2{\pm}25.9{\mu}m$, $186.8{\pm}70.6{\mu}m$, respectively). In the distal line angle, significant difference was found between the $1^{st}$, $2^{nd}$, $3^{rd}$ milling groups and the $4^{th}$ milling group. In the mesial axial wall, significant difference was found between the $1^{st}$ milling group ($52.2{\pm}20.3{\mu}m$) and the $3^{rd}$, $4^{th}$ milling groups ($22.8{\pm}8.8{\mu}m$, $7.8{\pm}5.7{\mu}m$). Conclusion: As a result of the experiment, decrease of the marginal and internal fit was statistically significant as the number of machining cycles increased. In order to produce clinically excellent restorations, it is recommandable to consider the condition of the milling tool wear, when designing the restoration with CAD program.

• Biomolecules & Therapeutics
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• v.30 no.6
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• pp.616-624
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• 2022

Mebendazole (MBZ), a microtubule depolymerizing drug commonly used for the treatment of helminthic infections, has been suggested as a repositioning candidate for the treatment of brain tumors. However, the efficacy of MBZ needs further study to improve the beneficial effect on the survival of those patients. In this study, we explored a novel strategy to improve MBZ efficacy using a drug combination. When glioblastoma cells were treated with MBZ, cell proliferation was dose-dependently inhibited with an IC₅₀ of less than 1 µM. MBZ treatment also inhibited glioblastoma cell migration with an IC₅₀ of less than 3 µM in the Boyden chamber migration assay. MBZ induced G2-M cell cycle arrest in U87 and U373 cells within 24 h. Then, at 72 h of treatment, it mainly caused cell death in U87 cells with an increased sub-G1 fraction, whereas polyploidy was seen in U373 cells. However, MBZ treatment did not affect ERK1/2 activation stimulated by growth factors. The marked induction of autophagy by MBZ was observed, without any increased expression of autophagy-related genes ATG5/7 and Beclin 1. Co-treatment with MBZ and the autophagy inhibitor chloroquine (CQ) markedly enhanced the anti-proliferative effects of MBZ in the cells. Triple combination treatment with temozolomide (TMZ) (another autophagy inducer) further enhanced the anti-proliferative effect of MBZ and CQ. The combination of MBZ and CQ also showed an enhanced effect in TMZ-resistant glioblastoma cells. Therefore, we suggest that the modulation of protective autophagy could be an efficient strategy for enhancing the anti-tumor efficacy of MBZ in glioblastoma cells.

• Journal of Korean Society of Steel Construction
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• v.10 no.4 s.37
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• pp.589-600
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• 1998

This study was planned to verify the usefulness of Latin square design method in fatigue tests of cables and to see the axial fatigue behavior of wire ropes being used as hangers in suspension bridges. Three parameters : mean stress, stress range. and specimen length, were adopted for verification. The effects of these parameters are in argument except for stress range. Three classes in each parameter were used. Triple replication was performed in each cell to increase the number of replication (or degree of freedoms). The major cause of fatigue failure was fretting fatigue at trellis contact point. Three chosen parameters were proved to be significant. It was verified that the effect of stress range was in agreement with expectation, but the effect of specimen length was contrary to the expectation. It was also observed that the effect of mean stress depended upon the chosen level. Therefore Latin square design method is effective for verifying the parameters that affect fatigue behaviour under orthogonality conditions.

• KSBB Journal
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• v.16 no.6
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• pp.579-591
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• 2001

The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.

• Mass Spectrometry Letters
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• v.12 no.2
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• pp.53-58
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• 2021

Previous in vitro studies have demonstrated that ginsenoside Rc inhibits UGT1A9, but there are no available data to indicate that ginsenoside Rc inhibits UGT1A9 in vivo. The effect of single and repeated intravenous injection of ginsenoside Rc was evaluated on the pharmacokinetics of mycophenolic acid. After injection of ginsenoside Rc (5 mg/kg for one day or 3 mg/kg for five days), 2-mg mycophenolic acid was intravenously injected, and the pharmacokinetics of mycophenolic acid and mycophenolic acid-β-glucuronide were determined. Concentrations of mycophenolic acid and its metabolite from rat plasma were analyzed using a liquid chromatography-triple quadrupole mass spectrometry. Single or repeated pretreatment with ginsenoside Rc had no significant effects on the pharmacokinetics of mycophenolic acid (P > 0.05): The mean difference in maximum plasma concentration (C_max) and area under the concentration-time curve (AUC_inf) were within 0.83- and 0.62-fold, respectively, compared with those in the absence of the ginsenoside Rc. These results indicate that ginsenoside Rc has a negligible effect on the disposition of mycophenolic acid in vivo despite in vitro findings indicating that ginsenoside Rc is a selective UGT1A9 inhibitor. As a result, ginsenoside Rc has little possibility of interacting with drugs that are metabolized by UGT1A9, including mycophenolic acid.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.303-307
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• 2007

This clinical experience reports the effect of wood and fire acupoints therapy on a hypertensive patient. Wood acupoint indicates Jing point in yin channel or Shu point in yang channel. Fire acupoint is Jing in yin channel or Jing in yang channel among the five acupoints of Jing, Xing, Shu, Jing and He. The patient was treated with indicated acupoints of 7 channels: lung, large intestine, spleen, pericardium, triple warmer, heart and liver, for 5 weeks. In case of necessity, another acupoints were added with wood and fire acupoints. After 5 weeks treatment, the blood pressure improved by 125/80 which is in normal level. This result suggests that wood and fire acupoints therapy is effective in the treatment of hypertension.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.3
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• pp.47-52
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• 2011

Directional couplers based on plasmonic waveguides with nano-scale three channels are designed by utilizing mode coupling effect as well as rib-guiding structure. Longitudinal modal transmission-line theory(L-MTLT) is used for simulating the light propagation and optimizing the design parameters. The designed plasmonic coupler operating as power splitter has nano-scale size of about 200~250 nm width. In order to achieve the desired power splitting ratio, the refractive index of guiding modes is evaluated along the width variation of center channel. Finally, a power splitter based on triple rib directional coupler, which ensures maximum power transfer from one outermost guide to the other outermost guide, is designed.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.14 no.4
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• pp.213-217
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• 2014

In this paper, we designed monopole microstrip antenna for WLAN/WiMAX system. The monopole antenna is designed by FR-4 substrate with size is $30mm{\times}40mm$. The proposed antenna is based on a planar monopole design which cover WLAN and WiMAX frequency bands. To obtain the optimized parameters, we used the simulator, CST's Microwave Studio Program and found the parameters that greatly effect antenna characteristics. Using the obtained parameters, the antenna is designed. Thus the proposed antenna satisfied the -10 dB impedance bandwidth requirement while simultaneously covering the WLAN and WiMAX bands. And characteristics of gain and radiation patterns are obtained for WLAN/WiMAX frequency bands.