• 제목/요약/키워드: Triple Life

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Glut1 promotes cell proliferation, migration and invasion by regulating epidermal growth factor receptor and integrin signaling in triple-negative breast cancer cells

  • Oh, Sunhwa;Kim, Hyungjoo;Nam, KeeSoo;Shin, Incheol
    • BMB Reports
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    • 제50권3호
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    • pp.132-137
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    • 2017
  • Elevated glucose levels in cancer cells can be attributed to increased levels of glucose transporter (GLUT) proteins. Glut1 expression is increased in human malignant cells. To investigate alternative roles of Glut1 in breast cancer, we silenced Glut1 in triple-negative breast-cancer cell lines using a short hairpin RNA (shRNA) system. Glut1 silencing was verified by Western blotting and qRT-PCR. Knockdown of Glut1 resulted in decreased cell proliferation, glucose uptake, migration, and invasion through modulation of the EGFR/MAPK signaling pathway and integrin ${\beta}1$/Src/FAK signaling pathways. These results suggest that Glut1 not only plays a role as a glucose transporter, but also acts as a regulator of signaling cascades in the tumorigenesis of breast cancer.

3중 점용접재의 귤열단 열림각(CTOA)을 이용한 피로균열거동 (Fatigue Crack Behavior of Triple Piece Spot by Crack Tip Opening Angle of Welded Specimen)

  • 송삼홍;주동호;양윤진
    • 한국정밀공학회지
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    • 제18권5호
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    • pp.74-83
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    • 2001
  • In this study, internal fatigue crack initiation and propagation behavior were investigated by triple piece spot welded specimen. To estimate fatigue life of the specimen varied with shape and thickness, Crack tip opening angle(CTOA) correlated with stress intensity factor was used as the stiffness parameter. The relation between fatigue life and CTOA can be arranged by the quantitative equation for each specimen by experiment. In addition, the variation of stress distribution was solved and the effect on fatigue crack behavior was examined by finite element method(FEM).

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Living Labs as boundary-spanners between Triple Helix actors

  • van Geenhuizen, Marina
    • Journal of Contemporary Eastern Asia
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    • 제15권1호
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    • pp.78-97
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    • 2016
  • Living labs are an increasingly popular methodology to enhance innovation. Living labs aim to span boundaries between different organizations, among others Triple helix actors, by acting as a network organization typically in a real-life environment to foster co-creation by user-groups. This paper presents critical factors of Living labs in boundary-spanning between Triple Helix actors. Derived from a mixed-method approach and applications in the healthcare sector, the three main critical factors turn out to be 1) an adequate user-group selection and involvement, specifically a rich interaction and absorption of its results, 2) a balanced involvement of all relevant actors, and 3) a sufficient (early) attention for values, both values of user-groups and values of the management. People-oriented Living labs tend to differ from institution-oriented Living labs regarding these critical factors. Further, universities tend to take on diverse roles and strength of involvement, while the business sector tends to be actively involved only if this has been set as an explicit aim at start. The paper closes with a summary and future research paths.

BLT2, a leukotriene B4 receptor 2, as a novel prognostic biomarker of triple-negative breast cancer

  • Park, JaeIn;Jang, Jae-Hyun;Park, Geun-Soo;Chung, Yunro;You, Hye Jin;Kim, Jae-Hong
    • BMB Reports
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    • 제51권8호
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    • pp.373-377
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    • 2018
  • Triple-negative breast cancer (TNBC) is considered to be a notorious type of cancer due to its aggressive metastatic potential and poor prognosis. Recent evidence suggests that BLT2, a low-affinity $LTB_4$ receptor is critically associated with the phenotypes of TNBC cells, including invasion, metastasis, and survival. Furthermore, in a group of 545 breast cancer patients with metastasis, we observed that the high-BLT2 subgroup had a lower disease-free-survival rate than the low-BLT2 subgroup. Thus, we theorized that anti-BLT2 strategies could facilitate the development of new therapies used for TNBC. This review focuses on recent discoveries regarding BLT2 and its roles in as a novel prognostic biomarker in TNBC.

KPNA3 promotes epithelial-mesenchymal transition by regulating TGF-β and AKT signaling pathways in MDA-MB-231, a triple-negative breast cancer cell line

  • Jaesung Choi;Jee-Hye Choi;Ho Woon Lee;Dongbeom Seo;Gavaachimed Lkhagvasuren;Jung-Woong Kim;Sang-Beom Seo;Kangseok Lee;Kwang-Ho Lee
    • BMB Reports
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    • 제56권2호
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    • pp.120-125
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    • 2023
  • Karyopherin-α3 (KPNA3), a karyopherin-α isoform, is intimately associated with metastatic progression via epithelial-mesenchymal transition (EMT). However, the molecular mechanism underlying how KPNA3 acts as an EMT inducer remains to be elucidated. In this report, we identified that KPNA3 was significantly upregulated in cancer cells, particularly in triple-negative breast cancer, and its knockdown resulted in the suppression of cell proliferation and metastasis. The comprehensive transcriptome analysis from KPNA3 knockdown cells indicated that KPNA3 is involved in the regulation of numerous EMT-related genes, including the downregulation of GATA3 and E-cadherin and the up-regulation of HAS2. Moreover, it was found that KPNA3 EMT-mediated metastasis can be achieved by TGF-β or AKT signaling pathways; this suggests that the novel independent signaling pathways KPNA3-TGF-β-GATA3-HAS2/E-cadherin and KPNA3-AKT-HAS2/E-cadherin are involved in the EMT-mediated progress of TNBC MDA-MB-231 cells. These findings provide new insights into the divergent EMT inducibility of KPNA3 according to cell and cancer type.

Outcome of Inhaler Withdrawal in Patients Receiving Triple Therapy for COPD

  • Kim, Sae Ahm;Lee, Ji-Hyun;Kim, Eun-Kyung;Kim, Tae-Hyung;Kim, Woo Jin;Lee, Jin Hwa;Yoon, Ho Il;Baek, Seunghee;Lee, Jae Seung;Oh, Yeon-Mok;Lee, Sang-Do
    • Tuberculosis and Respiratory Diseases
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    • 제79권1호
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    • pp.22-30
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    • 2016
  • Background: The purpose of this study was to document outcomes following withdrawal of a single inhaler (step-down) in chronic obstructive pulmonary disease (COPD) patients on triple therapy (long-acting muscarinic antagonist and a combination of long-acting ${\beta}2$-agonists and inhaled corticosteroid), which a common treatment strategy in clinical practice. Methods: Through a retrospective observational study, COPD patients receiving triple therapy over 2 years (triple group; n=109) were compared with those who had undergone triple therapy for at least 1 year and subsequently, over 9 months, initiated inhaler withdrawal (step-down group, n=39). The index time was defined as the time of withdrawal in the step-down group and as 1 year after the start of triple therapy in the triple group. Results: Lung function at the index time was superior and the previous exacerbation frequency was lower in the step-down group than in the triple group. Step-down resulted in aggravating disease symptoms, a reduced overall quality of life, decreasing exercise performance, and accelerated forced expiratory volume in 1 second ($FEV_1$) decline ($54.7{\pm}15.7mL/yr$ vs. $10.7{\pm}7.1mL/yr$, p=0.007), but there was no observed increase in the frequency of exacerbations. Conclusion: Withdrawal of a single inhaler during triple therapy in COPD patients should be conducted with caution as it may impair the exercise capacity and quality of life while accelerating $FEV_1$ decline.

소아의 Triple A 증후군의 변형 1례 (A Case of Triple A Syndrome)

  • 한재혁;유지형;이창한;정기섭
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • 제3권2호
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    • pp.188-194
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    • 2000
  • 반복되는 구토로 인한 폐렴 증세로 내원하여 분문무이완증, 무루증, 신경인성방광 등의 소견을 보여 Triple A 증후군으로 진단받고, 식도하부 괄약근의 반복 확장술(pneumatic dilatation)로 증상이 소실된 환아 1례를 경험하였기에 이에 보고하는 바이다.

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Microtubule Acetylation-Specific Inhibitors Induce Cell Death and Mitotic Arrest via JNK/AP-1 Activation in Triple-Negative Breast Cancer Cells

  • Suyeon Ahn;Ahreum Kwon;Youngsoo Oh;Sangmyung Rhee;Woo Keun Song
    • Molecules and Cells
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    • 제46권6호
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    • pp.387-398
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    • 2023
  • Microtubule acetylation has been proposed as a marker of highly heterogeneous and aggressive triple-negative breast cancer (TNBC). The novel microtubule acetylation inhibitors GM-90257 and GM-90631 (GM compounds) cause TNBC cancer cell death but the underlying mechanisms are currently unknown. In this study, we demonstrated that GM compounds function as anti-TNBC agents through activation of the JNK/AP-1 pathway. RNA-seq and biochemical analyses of GM compound-treated cells revealed that c-Jun N-terminal kinase (JNK) and members of its downstream signaling pathway are potential targets for GM compounds. Mechanistically, JNK activation by GM compounds induced an increase in c-Jun phosphorylation and c-Fos protein levels, thereby activating the activator protein-1 (AP-1) transcription factor. Notably, direct suppression of JNK with a pharmacological inhibitor alleviated Bcl2 reduction and cell death caused by GM compounds. TNBC cell death and mitotic arrest were induced by GM compounds through AP-1 activation in vitro. These results were reproduced in vivo, validating the significance of microtubule acetylation/JNK/AP-1 axis activation in the anti-cancer activity of GM compounds. Moreover, GM compounds significantly attenuated tumor growth, metastasis, and cancer-related death in mice, demonstrating strong potential as therapeutic agents for TNBC.

TripleDiff: 트리플 저장소에서 RDF 문서에 대한 점진적 갱신 알고리즘 (TripleDiff: an Incremental Update Algorithm on RDF Documents in Triple Stores)

  • 이태휘;김기성;유상원;김형주
    • 한국정보과학회논문지:데이타베이스
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    • 제33권5호
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    • pp.476-485
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    • 2006
  • 시멘틱 웹(semantic web)과 함께 등장한 RDF는 웹 상의 메타데이타 및 데이타를 나타내는 표준으로 자리매김 하고 있다. 이에 따라 RDF에 대한 저장 및 질의 처리에 대한 연구가 많이 이루어졌으며, 대표적인 시스템으로 Sesame, Jena 등이 있다. 그러나 아직 갱신 방법에 대한 연구는 부족하다. RDF 데이타가 지속적으로 갱신이 이루어지는 경우에는 저장된 RDF를 갱신해야 하는 상황이 발생한다. 현존하는 RDF 저장소에서 데이타를 갱신하기 위해서는 기존의 데이타를 모두 삭제한 후 새로운 데이타를 처음부터 다시 저장해야 하는데, 이러한 상황에서는 매우 비효율적이다. 또한 한 RDF 저장소에 여러 RDF가 저장되어 있는 경우에는 갱신 문제가 더욱 복잡해진다. 이에 본 논문에서는 RDF 데이타를 점진적으로 갱신하는 기법을 제안하고자 한다. 제안한 기법은 텍스트 비교 알고리즘을 통해 얻은 결과를 보완하여 기존 RDF 데이타에서 변화된 트리플 문장만을 추출하여 갱신한다. 실제 RDF 데이터를 이용한 실험을 통해 제안한 방법을 사용하여 갱신을 효율적으로 할 수 있음을 보였다.

The Global Knowledge Linkage Structures of the Agricultural Sector Pertinent to Information Technology: A Triple Helix Perspective

  • Hossain, Md. Dulal;Moon, Junghoon;Choe, Young Chan
    • Agribusiness and Information Management
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    • 제3권1호
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    • pp.23-37
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    • 2011
  • The development of informatization impacts all sectors, including agriculture. Agricultural informatization builds the knowledge linkage structures of agricultural innovation systems globally. This study investigated the global knowledge linkage structures in agricultural innovation pertinent to information technology (IT) for agricultural research and development (R&D) investments and activities. We explored the longitudinal trend of systemness within the networked research relationships in the triple helix (TH) of the university, industry and government (UIG). We collected data from publications in the Science Citation Index (SCI), the Social Sciences Citation Index (SSCI), and the Arts and Humanities Citation Index (A&HCI) to analyze the TH network dynamics. We also performed a scientometrics analysis to quantitatively identify the knowledge and insights of global agricultural innovation structures. These results could be informative for individual countries. Our findings reveal that the global knowledge linkage structures in the agricultural sector that are pertinent to IT fluctuate widely and fail to increase the capacity of agricultural innovation research due to a neglect of the network effects of the TH dynamics of UIG.

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