• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 춘계학술대회
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• pp.157-159
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• 2015

대부분의 중소기업 화물 트럭운전사들은 운송 관련 정보가 담긴 종이로 된 문서를 사용하여 작업을 처리하는 경우가 많다. 그러나 이러한 문서는 경로를 계획하거나 화물 내역을 확인함에 있어 분실과 훼손 등의 문제점으로 인해 불편함을 있다. 또한 종이가 아닌 기기를 사용하여 관리하기에는 중소기업으로써는 예산이 부족할 가능성이 있다. 이러한 문제점을 해결하기 위해서 본 논문에서는 스마트폰 애플리케이션과 관리자 프로그램을 통한 화물 운송 관리시스템을 구현하였다. 본 시스템은 기본적으로 데스크 탑 애플리케이션을 통해 관리자에게 쉬운 인터페이스를 통한 운송 정보 관리와 안드로이드 애플리케이션을 통해 화물트럭 운전사가 할당받은 화물 운송 정보, 길 찾기, 전화걸기, 운송할 물품의 수량 관리 등을 제공하여 화물 운송에 효율을 높이기 위해 구현하였다.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.131-138
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• 2017

This study was conducted to investigate stimulatory effect of epidermal growth factor (EGF) on nuclear maturation and the expression level of EGF-receptor (EGFR), GM-130 (a marker of Golgi apparatus), transport protein Sec61 subunit beta ($Sec61{\beta}$), and coatomer protein complex subunit gamma 2 (COPG2) in porcine oocytes. The cumulus-oocyte complexes were collected from follicle with 3-6 mm in diameter. They were incubated in medium with/without EGF for 22 h (IVM I) and subsequently incubated hormone-free medium with/without EGF for 22 h (IVM II). Nuclear maturation state was checked by aceto-orcein stain. Protein expression of EGFR, GM-130, $Sec61{\beta}$, and COPG2 were measured by immunofluorescence. In results, nuclear maturation of oocytes in EGF non-treated oocytes were significantly lower than EGF-treated groups at IVM I or IVM II stage (P<0.05), whereas maturational rate in EGF treatment groups at both of IVM stage was higher in among the all treatment groups (P<0.05). EGFR, GM-130, $Sec61{\beta}$ and COPG2 were expressed in the cytoplasm of oocytes. Especially, GM-130 and EGFR were strongly expressed, but $Sec61{\beta}$ and COPG2 were weakly expressed in cortical area of cytoplasm. The protein level of GM-130, $Sec61{\beta}$, and COPG2 were significantly higher in the EGF-treated groups (P<0.05). However EGFR was no difference between non EGF-treated groups and control. In conclusion, EGF plays an important role in the systems for oocyte maturation with endoplasmic reticulum and Golgi apparatus. In addition, the protein levels of $Sec61{\beta}$ and COPG2 could be changed by EGF in the porcine oocytes during maturation.

• Journal of Nutrition and Health
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• 제33권6호
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• pp.660-667
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• 2000

Previous studies on the effect of age on intestinal taurine transport in animals have invariably shown a decline in the activity of the transport system with increasing age. In the present study changes in taurine transporter activity were observed during cell differentiation in the human colon carcinoma cell line HT-29 This cell line exhibits various enterocytic characteristics when differentiated and therefore has frequently been used to study the characteristcs and regulation of nutrient and drug absorption in the small intestine at the cellular level. Pre-treatment of the cells with $\beta$-alanine(10mM) reduced the taurine transport activity to 33% of the value for the control cells(p<0.05) which implies that taurine and $\beta$-alanine share a common $\beta$-amino acid transport system for their celluar uptake in the HI-29 was continued until 21 days post seeding. Kinetic studies of the taurine transporter were conducted in the HT-29 cell line with varying taurine concentration(5-60$\mu$M) in the uptake medium Both Vmax and the Michaelis-Menten constant(Km) of taurine transporter were decreased as differentiation of the HT-29 cell line was progressed ; Vmax of the taurine transporter in cells incubated for 4, 14 and 21 days post seeding was 2.79$\pm$3.4m 16.89$\pm$1.74, and 0.85$\pm$0.08 and 0.32$\pm$0.01nmol.mg protein-1 .30min-1 respectively(p<0.001) and Km was 42.3$\pm$3.4, 16.89$\pm$1.74, and 11.2$\pm$3.0$\mu$M respectively (p<0.01) These results indicate that the activity of sodium dependent active taurine transport system in the HT-29 cell line is decreased as confluent cells are differentiated. This phenomenon in cell culture system corresponds well with the earlier observation of lower intestinal taurine transport activity in suckling rats compared to that in adult animals although direct relationship of cell differentiation with in vivo aging process needs further verification.

• Environmental Analysis Health and Toxicology
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• 제22권3호
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• pp.227-233
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• 2007

It has been reported that hepatic glutathione (GSH) levels are decreased in diabetic patients, and glucagon increases hepatic efflux of GSH into blood. The signaling pathways responsible for mediating the glucagon effects on GSH efflux, however, are unknown. The signaling pathways involved in the regulation of GSH efflux in response to glucagon and insulin were examined in primary cultured rat hepatocytes. The GSH concentrations in the culture medium were markedly increased by the addition of glucagon, although cellular GSH levels are significantly decreased by glucagon. Insulin was also increased the GSH concentrations in the culture medium, but which is reflected in elevations of both cellular GSH and protein. Treatment of cells with 8-bromo-cAMP or dibutyryl-cAMP also resulted in elevation of the GSH concentrations in the culture medium. Pretreatment with H89, a selective inhibitor of protein kinase A, before glucagon addition markedly attenuated the glucagon effect. These results suggest that glucagon changes GSH homeostasis via elevation of GSH efflux, which may be responsible for decrease in hepatic GSH levels observed in diabetic condition. Furthermore, the present study implicates cAMP and protein kinase A in mediating the effect of glucagon on GSH efflux in primary cultured rat hepatocytes.

• 한국ITS학회 논문지
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• 제7권4호
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• pp.107-117
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• 2008

본 논문에서는 국내 ETC(Electronic Toll Collection)인 한국도로공사의 하이패스 시스템의 보급에 힘입어 2008년 현재까지 약 125만대가 보급되어 있는 IR(Infrared)/RF(Radio Frequency)-DSRC(Dedicated Short Range Communication)를 BIS(Bus Information System) 혹은 UTIS(Urban Traffic Information System)와 같은 보다 향상되고, 많은 차량 단말(OBE; On Board Equipment)을 제어해야 하는 시스템에 사용하기 위한 척도로써 IR/RF-DSRC의 전송 효율에 관한 성능을 시뮬레이션을 통해 평가하고, 각 시스템의 장단점을 찾아보고자 한다. 특히 DSRC는 짝은 통신 영역에서 높은 이동도를 갖는 개체에게 고속의 데이터를 전송하여야 한다는 점에서 일반적인 통신 시스템에 비해 MAC(Media Access Control)의 속성이 가장 중하다고 할 수 있다. 따라서 본 논문에서는 이러한 매체 접근성의 관점에서 IR/RF-DSRC, 두 시스템의 성능을 분석하고자 한다.

• Journal of Ginseng Research
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• 제7권2호
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• pp.102-107
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• 1983

The effects of saponin, one of major components (Panax ginseng C.A. Meyer), on the growth of E. coli K-12 and the formation of siderphore was observed The following results were obtained. 1. When E. coli was grown on medium containing 1${\times}$10-5%-11${\times}$10-1% of the saponin, the rate of growth was stimulated at 10-1% of the saponin significantly compared to that of control. 2. When E. coli K-12 was grown on medium containing 1${\times}$10-1% of the saponin, the amount of siderphore was two times as much as the control. 3. The growth of E. coli was observed to be dependent on the concentration of siderophore when siderophore was added to medium. 4. The effect of saponin on the formation of siderophore in vitro was observed to reach maximum at 1${\times}$10-3% of the saponin. Such results suggest that the growth rate of E. coli K-12 could be enhanced by ginseng saponin fraction through stimulation of siderphore formation. We have described the fast growth of E. coli, K-12 and B. subtilis, rapid uptake of 14C-glucose, and high level of other metabolites such as lipids and proteins of E. coli, and B. subtilis in medium containing saponing fraction compared to that of microorganisms without saponin fraction.1∼3Such differences were claimed to be due to rapid uptake of 14C-glucose by widened periplasmic region throught unknown mechanism in the prescence of saponin fraction in medium3 and have raised a question whether there is another possible factor, siderophore4(Greek for iron bears), since microorganisms must secure a sufficient amount of iron for normal growth. These are known to be synthesized by the cells under iron-deficient condition and in most case, excreted into the medium5, where they can complex and solubilize any iron present there. It is generally believed that these complexes are then taken into the cells presumably by specific transport systems, thus providing iron for cell metabolism. Within the group of enteric bacteria, only three species (E. coli, S. typhimurium, and A. aerogense) have, so far, been studied in a ny detail. The main iron-binding compound produced by these species is enterochelin, and its role in iron transport is now well established. And biosynthesis of enterochelin from 2, 3- dihydroxybenzoate and serine in the prescence of magnesium ions and ATP was reported6. 2, 3-dihydroxybenzoate was also shown to involve isochorismate and 2, 3-dihydro-2, 3-dihydroxybenzoate as intermediate.7∼11 The present paper deals with the effect of ginseng saponin fraction on growth, the level of enterochelin formation in vivo and the conversion of 2, 3-dihydroxybenzoate and serine into entrochelin in vitro, and entrochelin obtained on the growth in relation to possible explanation of ginseng saponin fraction on the rapid growth of E. coli, K-12.

• The Korean Journal of Physiology
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• 제29권1호
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• pp.69-80
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• 1995

Renal proximal tubular hypertrophy and hyperfunction are known to be early manifestations of experimental and human diabetes. As the hypertrophy and hyperfunction have been suggested to be central components in the progression to renal failure, an understanding of their underlying causes is potentially important for the development of therapy. A primary rabbit kidney proximal tubule cell culture system was utilized to evaluate the possibility that the renal proximal tubular hypertrophy and hyperfunction observed in vivo in diabetes mellitus, can be attributed to effects of elevated glucose levels on membrane transport systems. Primary cultures of rabbit proximal tubules, which achieved confluence at 10 days, exhibited brush-border characteristics typical of proximal tubular cells. Northern analysis indicated $2.2{\sim}2.3$ and 2.0 kb Na/glucose cotransporter RNA species appeared in fresh and cultured proximal tubule cells after confluence, repectively. The cultured cells showed reduced Na/glucose cotransporter activity compared to fresh proximal tubules. Primary cultured proximal tubule cells incubated in medium containing 20 mM glucose have reduced ${\alpha}-MG$ transport compared to cells grown in 5 mM glucose. In the proximal tubule cultures incubated in medium containing 5 mM or 20 mM glucose, phlorizin at 0.5 mM inhibited 0.5 mM ${\alpha}-MG$ uptake by 84.35% or 91.85%, respectively. The uptake of 0.5 mM ${\alpha}-MG$ was similarly inhibited by 0.1 mM ouabain (41.97% or 48.03% inhibition was observed, respectively). In addition, ${\alpha}-MG$ uptake was inhibited to a greater extent when $Na^{+}$ was omitted from the uptake buffer (81.86% or 86.73% inhibition was observed, respectively). In cell homogenates derived from the primary cells grown in 5 mM glucose medium, the specific activity of the Na/K-ATPase $(6.17{\pm}1.27\;{\mu}mole\;Pi/mg\;protein/hr)$ was 1.56 fold lower than the values in cell homogenates treated with 360 mg/dl D-glucose, 20 mM $(9.67{\pm}1.22\;{\mu}mole\;Pi/mg\;protein/hr)$. Total $Rb^{+}$ uptake occurred at a significantly higher rate (1.60 fold increase) in primary cultured rabbit kidney proximal tubule cell monolayers incubated in 20 mM glucose medium $(10.48{\pm}2.45\;nM/mg\;protein/min)$ as compared with parallel cultures in 5 mM glucose medium. $Rb^{+}$ uptake rate in 5 mM glucose medium was reduced by 28% when the cultures were incubated with 1 mM ouabain. The increase of the $Rb^{+}$ uptake by rabbit kidney proximal tubule cells in 20 mM glucose could be attributed primarily to an increase in the rate of ouabain-sensitive $Rb^{+}$ uptake $(5\;mM\;to\;20\;mM;\;4.68{\pm}0.85\;to\;8.38{\pm}1.37\;nM/mg\;protein/min)$. In conclusion, the activity of the renal proximal tubular Na,K-ATPase is elevated in high glucose concentration. In contrast, the activity of the Nafglucose cotransport system is inhibited.

• 천문학회보
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• 제38권1호
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• pp.46.2-46.2
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• 2013

The neutral component of the interstellar medium (ISM) is segregated into the cold neutral medium (CNM) and warm neutral medium (WNM) as a result of thermal instability. It was found that the CNM--WNM evaporation interface, across which the CNM undergoes thermal expansion, is linearly unstable to corrugational disturbances, in complete analogy with the Darrieus-Landau instability (DLI) in terrestrial flames. To explore dynamical consequences of the DLI in the ISM, we perform a linear stability analysis of the DLI including the effect of thermal conduction as well as nonlinear hydrodynamic simulations. We find that the DLI is suppressed at short length scales via heat transport. The linear growth time of the fastest growing mode is proportional to the square of the evaporation flow speed of the CNM relative to the interface and is typically >10 Myr. In the nonlinear stage, perturbations grow into cusp-like structure protruding toward the WNM, and soon reach a steady state where the evaporation rate is increased by a factor of 2 compared to the initial state. We demonstrate that the amplitude of the interface distortion and enhancement in evaporation rate are determined primarily by the density ratio between the CNM and WNM. Given quite a long growth time and highly subsonic velocities at saturation, the DLI is unlikely to play an important role in the ISM dynamics.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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• pp.3268-3277
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• 2011

의왕ICD는 현대적 시설과 하역장비를 갖추어 연간 100만TEU를 처리 할 수 있는 수도권유일의 내륙컨테이너기지이다. 유리한 입지조건, 수도권 물류수송의 거점 역할, 내륙통관 기능 등 많은 이점을 가지고 있어 철도수송 활성화의 중요한 부분을 차지한다. 그러나 의왕ICD는 현재 시설 및 운영상의 문제점으로 컨테이너화물 철도수송 활성화의 한계에 있는 실정이다. 본 연구에서는 의왕ICD의 문제점을 시설부분과 운영부분으로 구분하여 분석하고, 2023년 점용허가 기간이 만료되는 시점을 기준으로 단기, 중장기로 구분하여 개선방안을 제시하였다. 그러나 의왕ICD의 철도수송 활성화는 이러한 개선방안 외에도 철도공사와 입주업체의 노력과함께, 정부, 의왕시, 철도시설공단 등 관련기관의 지원과 협력이 있어야만 가능 할 것이다. 이러한 의왕ICD의 개선은 복합일관수송체계를 구축하여 효율적인 철도물류 인프라 조성 및 철도물류 활성화에 기여하고, 또한 친환경 수송시스템 구축으로 정부의 저탄소 녹색성장 정책에도 기조 할 것으로 기대된다.

• 대한지하수환경학회지
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• 제2권1호
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• pp.14-21
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• 1995

지하수에 콜로이드가 있을 경우 지체계수 감소로인한 오염물의 가속이송이 일어난다. 세균은 콜로이드 크기이며, 비중이 물과 비슷하고. 또 특유의 표면조건으로 인하여 오염물을 가속이송시킬 수 있다. 유동 세균이 지하수에 있으면 그 system 은 액상, 고상, 그리고 입상의 3 상 (phase) 으로 해석되어야 한다. 본 연구에서는 물질수지에 기초한 수학적 모델을 개발하여 다공매질에서의 오염물의 이동을 예측했다. 세균과 오염물의 각 상사이의 분배는 평형분배 모델을 이용했다. 모델결과를 일반화하기 위하여 무차원화 기법이 사용되었다. 모델의 해는 수치해법이 사용되었고, 문헌상의 실험결과와 비교되었다. 유동 세균이 존재하므로써 오염물이 가속이송되었고, 오염물을 섭취하므로써 그 농도를 낮출 수 있었다.