• Journal of radiological science and technology
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• v.41 no.5
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• pp.427-435
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• 2018

There was a shortage of research reports on sterilization criterion and contamination of ultrasonic probes. Therefore, in this study, we were going to provide a basic study to measure the level of microbial contamination in ultrasonic probes and to investigate the radiographer's awareness of infection. After the scan, samples were collected from the rubber part of the probe by opening a sterile swab (Transport Medium AM608-1S) for medical bacteria collection with the remaining gel removed with a paper towel. Also, the collected samples of bacteria were grown for seven days and then the laboratory was analyzed. Among the total 29 types of microorganisms, Micrococcus luteus 21(26%), Moraxella species 16(20%), Coagulase negative staphylococcus 8(10%), Bacillus species 5(7%), Bicillus circulans 3(5%), Acinetobacter lwoffii 2(2%), and 1 other Candida parapsilosis (1%) a number of bacteria and fungus, was detected. In a disinfectant experiment using LuciPac Pen on the Lumitester PD-30s, we cultured the rubber part of the probe two to three times to measure the bacteria. Bacteria decreased to 97% with Aquanax (alkaline reduced water 100%), 99% with Klarion wash (0.01% sodium hydroxide), 94% with Klarion disinfection (0.01% nitrous acid water), Sterilization was best with Klarion wash (0.01% sodium hydroxide). Therefore, guidelines for cleaning and disinfection of ultrasonic probes was required, and further development of probe-only disinfectants is required.

• Advances in nano research
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• v.5 no.3
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• pp.245-251
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• 2017

Multiwalled carbon-nanotubes (MWCNT) and micro-structured carbon, such as biochar or activated carbon (AC), have been seen to significantly increase the growth indices of certain plant species such as maize (Zea mays L.). Seed imbibition is the stage where environmental factors that affect water transport across the seed coat barrier, make a large impact. This work explores the effect on water imbibition by maize seeds when the aqueous environment surrounding the seed is diluted by small concentrations (10 and 20 mg/l) of pristine MWCNT (p-MWCNT), carboxylate functionalized MWCNT (COO-MWCNT) and AC. The degree of sensitivity of the process to (i) large structural changes is seen by utilizing the nano (the MWCNT) and the micro (the AC) allotropic forms of carbon; (ii) to small changes in the purity and morphology of the p-MWCNT by utilizing 95% pure and 99% pure p-MWCNTs of slightly differing morphologies; and (iii) to MWCNT functionalization by using highly pure (97%) COO-MWCNT. Water imbibition was monitored over a 15 hour period by Near Infrared Thermography (NIRT) and also by seed weighing. Seed surface topography was seen by SEM imaging. Analysis of the NIRT images suggests rapid seed surface topological changes with the quantity of water imbibed. While further work is necessary to arrive at a conclusive answer, this work shows that the imbibition phase of the maize seed is sensitive to the presence of MWCNT even to small differences in the purity of the p-MWCNT and to small differences in the physicochemical properties of the medium caused by the hydrophilic COO-MWCNT.

• Journal of the Korean Geographical Society
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• v.28 no.3
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• pp.200-212
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• 1993

Wholesaling is important industry that is remarkable by the function and characteristics of city. This paper aims at examing the recent changes in hierarchy of cities and city classifi-cation by wholesale activities in Korea. In order to grasp the stydy purpose, this paper is to analuze the changes of categories by wholesale industry, and to grasp the change in the city classification with city hierachy of wholesale sales in 1968, 1979, and 1991. The data were obtained from the statistics in the Census of Wholesale and Retail Trade published by the National Statistical Office, in 1969, 1981, and 1992. As the result of examination, the following finding were obtained: 1. Wholesaling has developed form that of production and consumption goods wholesale, especially 'Wholesaling of Farm Products, Foods and Beverages' to that of investment goods that is 'Wholesaling of Machinery and Equipment' and 'Wholesaling of Transport Equipment and Parts'. 2. Wholesaling has developed in the medium and small cities in 1970's, and in the larger cities in 1980's. And the concentration ratio of six larger cities were lower than another cities in terms of wholesale sales, especially Seoul and Pusan. 3. Recently with income increment and a purchasing power, city classification was changed by the increasing number of wholesale establi-shments of 'Automotive Parts and Tyres' in 1970's and of 'Office, Computing and Accounting Machines and Motor Vehicles' in 1980's.

• Journal of Electrochemical Science and Technology
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• v.11 no.3
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• pp.273-281
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• 2020

Constructing and making highly active and stable nanostructured Pt-based catalysts with ultralow Pt loading are still electrifying for electrochemical applications such as water electrolysis and fuel cells. In this study, MoO₃ ribbons (RBs) of few micrometer in length is successfully synthesized via hydrothermal synthesis. Subsequently, 3-dimentional (3D)-silicate layer for about 10 to 15 nm is introduced via chemical deposition onto the pre-formed MoO₃ RBs; to setup the platform for Pt metaldots (MDs) deposition. In comparison with the bare MoO₃ RBs, the MoO₃-Si has served as a efficient solid-support for stabilizing and accommodating the uniform deposition of sub-2 nm Pt MDs. Such a structural design would effectively assist in improving the electronic conductivity of a fabricated MoO₃-Si-Pt catalyst towards MOR; the interfaced, porous and 3D silicate layer has assisted in an efficient mass transport and quenching the poisonous CO_ads species leading to a significant electrocatalytic performance for MOR in alkaline medium. Uniformly decorated, sub-2 nm sized Pt MDs has synergistically oxidized the MeOH in association with the MoO₃-Si solid-support hence, synergistic catalytic activity has been achieved. Present facile approach can be extended for fabricating variety of highly efficient Metal Oxide-Metal Nanocomposite for energy harvesting applications.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.6B
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• pp.527-533
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• 2006

In this paper, we analyze the performance of MAC (Media Access Control) layer in DSRC (Dedicated Short Range Communication). It will be widely applied for ITS (Intelligent Transportation System) services; for example ETC (Electric Toll Control), BIS (Bus Information System) etc., needed to small packet size. But If ITS service is evolving to advance ITS, ADIS (Advanced Driver Information Systems) and AVHS (Advanced Vehicle Highway System) etc, be needed larger packet size. In the future, it may offer more various services such as traffic information, collection, and multimedia information. There are two kind of physical media, IR(Infrared) and RF(Radio frequency). And each system has their own protocol that is adaptive in special characteristics of physical medium for using efficiently limited radio resources. In this paper, we analyze the special characteristics of each system. And we study practical use of some related services expected to be used in the near future, by analyzing the transmission efficiency in each DSRC system.

• Proceedings of the KSRS Conference
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• 2008.10a
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• pp.340-343
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• 2008

The Soya Warm Current (SWC) is a coastal boundary current, which flows along the coast of Hokkaido in the Sea of Okhotsk. Seasonal and subinertial variations in the SWC are investigated using data obtained by high-frequency (HF) ocean radars, coastal tide gauges, and a bottom-mounted acoustic Doppler current profiler (ADCP). The HF radars clearly capture the seasonal variations in the surface current fields of the SWC. The velocity of the SWC reaches its maximum, approximately 1 m/s, in the summer, and becomes weaker in the winter. The velocity core is located 20 to 30 km from the coast, and its width is approximately 50 km. The almost same seasonal cycle was repeated in the period from August 2003 to March 2007. In addition to the annual variation, the SWC exhibits subinertial variations with a period from 10-15 days. The surface transport by the SWC shows a significant correlation with the sea level difference between the Sea of Japan and Sea of Okhotsk for both of the seasonal and subinertial variations, indicating that the SWC is driven by the sea level difference between the two seas. Generation mechanism of the subinertial variation is discussed using wind data from the European Centre for Medium-range Weather Forecasts (ECMWF) analyses. The subinertial variations in the SWC are significantly correlated with the meridional wind component over the region. The subinertial variations in the sea level difference and surface current delay from the meridional wind variations for one or two days. Continental shelf waves triggered by the meridional wind on the east coast of Sakhalin and west coast of Hokkaido are considered to be a possible generation mechanism for the subinertial variations in the SWC.

• 한국전산유체공학회:학술대회논문집
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• 2008.03b
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• pp.666-669
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• 2008

Droplet in miniaturized microfluidic systems have received much focused attention recently. In this work, electrical charging phenomenon of a conducting water droplet on the electrode under the dc electric field is studied and using this phenomenon droplet actuation method for microreactor applications is experimentally demonstrated. To find effects of key factors, the effects of electric field, medium viscosity, and droplet size are investigated. A scaling law of charging for the conducting droplet is derived from the experimental results. Unlike the case of a perfect conductor, the estimated amount of electrical charge ($Q_{est}$) of a water droplet is proportional to the 1.59 power of the droplet radius (R) and the 1.33 power of the electric field strength (E). (For a spherical perfect conductor, Q is proportional to R2 and E.) It is thought that the differences are mainly due to incomplete charging of a water droplet resulted from the combined effect of electrochemical reaction at electrode and the relatively low conductivity of water. Using this phenomenon, we demonstrate the transport of the charged droplet and fusion of two oppositely-charged droplets. When electric field is subjected sequentially on the electrode, the charged droplet is transported on the electrode. For the visualization of fusion of charged droplets, the precipitation reaction is used. When subjected to a DC voltage, two droplets charged are moving and merging toward each other due to the Coulombic force and chemical reaction is simultaneously occurred by coalescence of droplets. It may be due to the interchange effect of charge. It is shown that the droplet can be used for microreactor where transporting, merging etc. of reagents constitute unit operation.

• Biomedical Science Letters
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• v.7 no.4
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• pp.161-166
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• 2001

Most mammalian cells take up glucose by passive transport proteins in the plasma membranes. The best known of these proteins is the human erythrocyte glucose transporter, GLUT1. High levels of heterologous expression far the transporter are necessary for the investigation of its three-dimensional structure by crystallization. To achieve this, the baculovirus expression system has become popular choice. However, Spodoptera frugiperda Clone 9 (Sf9) cells, which are commonly employed as the host permissive cell line to support baculovirus replication and protein synthesis, grow well on TC-100 medium that contains 0.1% D-glucose as the major carbon source, suggesting the presence of endogenous glucose transporters. Furthermore, very little is known of the endogenous transporters properties of Sf9 cells. Therefore, human GLUT1 antibodies would play an important role for characterization of the GLUT1 expressed in insect cell. However, the successful use of such antibodies for characterization of GLUT1 expression m insect cells relies upon their specificity for the human protein and lack of cross-reaction with endogenous transporters. It is therefore important to determine the potential cross-reactivity of the antibodies with the endogenous insect cell glucose transporters. In the present study, the potential cross-reactivity of the human GLUT1 antibodies with the endogenous insect cell glucose transporters was examined by Western blotting. Neither the antibodies against intact GLUT1 nor those against the C-terminus labelled any band migrating in the region expected fur a protein of M$_r$ comparable to GLUT1, whereas these antibodies specifically recognized the human GLUT1. Specificity of the human GLUT1 antibodies tested was also shown by cross-reaction with the GLUT1 expressed in insect cells. In addition, the insect cell glucose transporter was found to have very low affinity for cytochalasin B, a potent inhibitor of human erythrocyte glucose transporter.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.109-114
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• 2008

A dynamic model has been developed to simulate dynamic operation of a real double-effect absorption chiller. Dynamic behavior of working fluids in main components was modeled in first-order nonlinear differential equations based on heat and mass balances. Mass transport mechanisms among the main components were modeled by valve throttling, 'U' tube overflow and solution sub-cooling. The nonlinear dynamic equations coupled with the subroutines to calculate thermodynamic properties of working fluids were solved by a numerical method. The dynamic performance of the model was compared with the test data of a commercial medium chiller. The model showed a good agreement with the test data except for the first 5,000 seconds during which different flow rates of the weak solution caused some discrepancy. It was found that the chiller dynamics is governed by the inlet temperatures of the cooling water and the chilled water when the heat input to the chiller is relatively constant.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.161-168
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• 2004

Optimal regeneration conditions of ginseng transformants were studied. It has been known that Ferritin Light Heavy Chain (FLHC) gene remove the several heavy metal by combination, store and transport. To obtain the ginseng tolerant to heavy metal, binary vector was introduced in Agrobacterium by tri-parental mating and then Agrobacterium tumefaciens MP90/FLHC was selected on the AB media and MS media containing kanamycin. Explants were co-cultured with Agrobacterium tumefaciens MP90/FLHC, which contained NPT II as a selectable marker, tadpole ferritin heavy chain (FLHC) gene and human ferritin light chain gene and then a number of embryos were induced. The induced embryo transferred to shooting media consisting of MS medium supplemented with GA 10 mg/L. As a result of examination that induced the normal growth of transfomants, transformants showed the equivalent growth in both root and shoot on the media containing the 1/3 MS.