• BMB Reports
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• v.49 no.8
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• pp.431-436
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• 2016

Human papillomavirus (HPV) is the major cause of cervical cancer, a deadly threat to millions of females. The early oncogene product (E7) of the high-risk HPV16 is the primary agent associated with HPV-related cervical cancers. In order to understand how E7 contributes to the transforming activity, we investigated the structural features of the flexible N-terminal region (46 residues) of E7 by carrying out N-15 heteronuclear NMR experiments and replica exchange molecular dynamics simulations. Several NMR parameters as well as simulation ensemble structures indicate that this intrinsically disordered region of E7 contains two transient (10-20% populated) helical pre-structured motifs that overlap with important target binding moieties such as an E2F-mimic motif and a pRb-binding LXCXE segment. Presence of such target-binding motifs in HPV16 E7 provides a reasonable explanation for its promiscuous target-binding behavior associated with its transforming activity.

• Journal of Environmental Health Sciences
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• v.19 no.3
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• pp.17-21
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• 1993

Characteristics of a downflow anaerobic packed bed reactor with raschig ring ceramics as a packing were measured and discussed for the basic evaluation of the process. A synthesized glucose substrate wastewater were used as a feed and process characteristics such as pH, biogas production, composition of produced gas, COD removal and VSS were measured with the hydraulic retention time (HRT) changing from 0.25 to 2 days. As a result, this type of reactor was applicable in continuous operation within the given HRT range and the transient period approaching the steady state was about 20 days. The content of methane in produced gas increase with HRT was always high above 50% enough to use as energy source. The COD removal efficiency increased gradually as HRT increased. The axial profile of VSS concentration in the reactor usually showed the maximum at the lower region and the minimum at the middle. The VSS concentration at the upper region and the exit appeared similarly. However, at 0.25 day of HRT, the VSS concentration of effluent became higher than that of the upper region. Therefore the optimum HRT of this reactor occurred about 0.5 day, at which the production of methane began to be just stabilized and loss of VSS and COD removal were resonable.

• Journal of Life Science
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• v.26 no.1
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• pp.42-49
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• 2016

The retinoic acid (RA) plays an important role in the growth and development of many cells, and bioactive RA concentration is regulated by several enzymes, including CYP26A1. The expression of the CYP26A1 gene is regulated by RA, and the CYP26A1 gene is one of the candidates for RA-responsive genes. Although CYP26A1 genes are cloned from several animals, cloning of the CYP26A1 gene from cows has not been reported yet. The promoter region of CYP26A1 from cows was cloned by PCR and analyzed by sequence alignment with human and mouse CYP26A1. The RA-responsive element (RARE), DR-5 (ttggg), was located in this region and was perfectly conserved. The promoter region of bovine CYP26A1, which contains DR-5, was ligated to the luciferase reporter gene on transient transfection assays. The expression of CYP26A1-Luc promoter was activated by ATRA treatment in lung-derived mtCC cells. Co-transfection with RAR-α or -β with ATRA significantly activates the expression of CYP26A1-Luc promoter; however, it was less effective with either RAR-γ or RXR-γ. In addition, the endogenous gene expressions measured by Q-RT-PCR in mtCC cells were not significantly affected by ATRA treatment for 2 days; however, the expression of the endogenous CYP26A1 gene was diminished sharply at day 3 with ATRA treatment. In conclusion, the promoter region of bovine CYP26A1 contains conserved DR-5 RARE, which functions as a binding site for RAR-α or -β, and it is involved in the regulation of CYP26A1 gene expression and the control of RA signaling in mtCC cells.

• Journal of Mechanical Science and Technology
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• v.17 no.6
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• pp.925-934
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• 2003

An experimental study is performed to investigate the characteristics of near wake flow behind a circular cylinder with serrated fins using a constant temperature anemometer and flow visualization. Various vortex shedding modes are observed. Fin height and pitch are closely related to the vortex shedding frequency after a certain transient Reynolds number. The through velocity across the fins decreases with increasing fin height and decreasing fin pitch. Vortex shedding is affected strongly by the velocity distribution just on top of the finned tube. The weaker gradient of velocity distribution is shown as increasing the freestream velocity and the fin height, while decreasing the fin pitch. The weaker velocity gradient delays the entrainment flow and weakens its strength. As a result of this phenomenon, vortex shedding is decreased. The effective diameter is defined as a virtual circular cylinder diameter taking into account the volume of fins, while the hydraulic diameter is proposed to cover the effect of friction by the fin surfaces. The Strouhal number based upon the effective diameters seems to correlate well with that of a circular cylinder without fins. After a certain transient Reynolds number, the trend of the Strouhal number can be estimated by checking the ratio of effective diameter to inner diameter. The normalized velocity and turbulent intensity distributions with the hydraulic diameter exhibit the best correlation with the circular cylinder's data.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.1
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• pp.1-8
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• 2003

The heat transfer and flow measurements on a cylindrical pedestal mounted on a flat surface with a turbulent impinging jet were made. The experiments were made for the jet Reynolds number of Re = 23,000, the dimensionless nozzle-to-surface distance of L/d = 2~10, the dimensionless pedestal height of H/D = 0~1.5. Measurements of the surface temperature and the Nusselt number distributions on the plate surface were made using liquid crystal and shroud-transient technique. Flow measurements involve smoke flow visualization and the wall pressure coefficient. The results show that the wall pressure coefficient sharply decreases along the upper surface of the pedestal. However, the pressure increases when the fluid escapes from the pedestal and then collides on the plate surface. The secondary maxima in the Nusselt numbers occur in the region of 1.0 $\leq$ r/d $\leq$ 1.9. Their values for the case of H/D = 0.5 are maximum 80% higher than those for other cases. The formation of the secondary maxima may be attributed to the reattachment of flow on the plate surface which was separated at the edge of the pedestal.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.4
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• pp.395-405
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• 2004

The filling pattern and an adaptive grid refinement based on the finite element method and Eulerian mesh advancement approach have been developed to analyze incompressible transient viscous flow with free surfaces. The governing equation for flow analysis is Navier-Stokes equation including inertia and gravity effects. The mixed FE formulation and predictor-corrector method are used effectively for unsteady numerical simulation. The flow front surface and the volume inflow rate are calculated using the filling pattern technique to select an adequate pattern among four filling patterns at each triangular control volume. By adaptive grid refinement, the new flow field that renders better prediction in flow surface shape is generated and the velocity field at the flow front part is calculated more exactly. In this domain the elements in the surface region are made finer than those in the remaining regions for more efficient computation. Using the proposed numerical technique, the collapse of a water dam has been analyzed to predict flow phenomenon of fluid and the predicted front positions with respect to time have been compared with the reported experimental results.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.14 no.3
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• pp.96-102
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• 2005

Acoustic Emission(AE) technique has been applied to not only material characterization evaluation but also on-line monitoring of the structural integrity. The AE source location technique is very important to identify the source, such as crack, leak detection. Since the AE waveforms obtained from sensors are very difficult to distinguish the defect signals, therefore, it is necessary to consider the signal analysis of the transient wave-form. In this study, we have divided the region of interest into a set finite elements, and calculated the arrival time differences between sensors by using the velocities at every degree from 0 to 90. A new technique for the source location of acoustic emission in fiberboard plates has been studied by introducing Wavelet Transform(WT) do-noising technique. WT is a powerful tool for processing transient signals with temporally varying spectra. If the WT de-noising was employed, we could successfully filter out the errors of source location in fiberboard plates by arrival time difference method. The accuracy of source location appeared to be significantly improved.

• Clinical and Experimental Pediatrics
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• v.54 no.4
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• pp.179-182
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• 2011

Transient neonatal diabetes mellitus (TNDM) is a rare form of diabetes mellitus that presents within the first 6 months of life with remission in infancy or early childhood. TNDM is mainly caused by anomalies in the imprinted region on chromosome 6q24; however, recently, mutations in the ABCC8 gene, which encodes sulfonylurea receptor 1 (SUR1), have also been implicated in TNDM. Herein, we present the case of a male child with TNDM whose mutational analysis revealed a heterozygous c.3547C>T substitution in the ABCC8 gene, leading to an Arg1183Trp mutation in the SUR1 protein. The parents were clinically unaffected and did not show a mutation in the ABCC8 gene. This is the first case of a de novo ABCC8 gene mutation in a Korean patient with TNDM. The patient was initially treated with insulin and successfully switched to sulfonylurea therapy at 14 months of age. Remission of diabetes had occurred at the age of 16 months. Currently, the patient is 21 months old and is euglycemic without any insulin or oral hypoglycemic agents. His growth and physical development are normal, and there are no delays in achieving neurological and developmental milestones.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.40 no.1
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• pp.12-22
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• 2012

Performance variation of autorotating rotor was investigated. The shaft angle of the rotor is reduced while the flight velocity is increased. The BO-105 helicopter rotor blade was replaced by untwisted NACA 0012 airfoil and the rotor was simulated by using Transient Simulation Method(TSM) to judge the autorotation region for the variables. To simulate the compressibility effect at high speed flight, two-dimensional aerodynamic data was analyzed by compressible Navier-Stokes solver and Pitt/Peters inflow theory was adopted to simulate the induced velocity field. Thrust and lift coefficients, lift to drag ratio variations were investigated, also the lift and power were compared to those of BO-105 helicopter. Sharing lift and power between the autorotating rotor and wing was considered when the compound aircraft concept is introduced.

• Journal of Powder Materials
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• v.9 no.3
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• pp.189-198
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• 2002

In this work, transient liquid phase (TLP) bonding of Ni-Cr heat resisted cast alloy (HP) was investigated. And also the behaviors of the solid particles distributed in the interlayer during TLP bonding were investigated. The MBF-60 and solid particles (Ni, Fe, and $Al_2O_3$ powders respectively) added MBF-60 which will be a liquid phase coexisting with solid particles at the bonding temperature were used as insert metal. The effective and sound bonding was possible by spark plasma sinter-bonding due to the differences of electric resistance between base metal and liquid insert layer which creates high temperature region. During the isothermal solidification, $Al_2O_3$ particles and solid particles of liquid phase sintered insert metal have shown no growth, while Ni and Fe particles grow rapidly. In this TLP bonding using the MBF-60 and distributed Fe, Ni particles as insert materials, the whole isothermal solidification process was dominated by the growth rate of the solid particles distributed in the interlayer.