• Title/Summary/Keyword: Transformation system

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Mannose-Based Selection with Phosphomannose-Isomerase (PMI) Gene as a Positive Selectable Marker for Rice Genetic Transformation

  • Penna, Suprasanna;Ramaswamy, Manjunatha Benakanare;Anant., Bapat Vishvas.
    • Journal of Crop Science and Biotechnology
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    • v.11 no.4
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    • pp.233-236
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    • 2008
  • A positive selectable marker system was adapted for transformation of mature embryo-derived calli of Indica rice (Oryza sativa L.) utilizing the PMI gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. The transformed cells grew on medium supplemented with 3% mannose as carbon source and calli were selected on media containing various concentrations of mannose. Molecular analyses showed that the transformed plants contained the PMI gene. The results indicate that the mannose selection system can be used for Agrobacterium-mediated transformation of mature embryo in rice to substitute the use of conventional selectable markers in genetic transformation.

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Detection of transgene in early developmental stage by GFP monitoring enhances the efficiency of genetic transformation of pepper

  • Jung, Min;Shin, Sun-Hee;Park, Jeong-Mi;Lee, Sung-Nam;Lee, Mi-Yeon;Ryu, Ki-Hyun;Paek, Kee-Yoeup;Harn, Chee-Hark
    • Plant Biotechnology Reports
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    • v.5 no.2
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    • pp.157-167
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    • 2011
  • In order to establish a reliable and highly efficient method for genetic transformation of pepper, a monitoring system featuring GFP (green fluorescent protein) as a report marker was applied to Agrobacteriummediated transformation. A callus-induced transformation (CIT) system was used to transform the GFP gene. GFP expression was observed in all tissues of $T_0$, $T_1$ and $T_2$ peppers, constituting the first instance in which the whole pepper plant has exhibited GFP fluorescence. A total of 38 T0 peppers were obtained from 4,200 explants. The transformation rate ranged from 0.47 to 1.83% depending on the genotype, which was higher than that obtained by CIT without the GFP monitoring system. This technique could enhance selection power by monitoring GFP expression at the early stage of callus in vitro. The detection of GFP expression in the callus led to successful identification of the shoot that contained the transgene. Thus, this technique saved lots of time and money for conducting the genetic transformation process of pepper. In addition, a co-transformation technique was applied to the target transgene, CaCS (encoding capsaicinoid synthetase of Capsicum) along with GFP. Paprika varieties were transformed by the CaCS::GFP construct, and GFP expression in callus tissues of paprika was monitored to select the right transformant.

Comparison Between DCM and Quaternion Transformation in Lever Arm Compensation of Reference System for Flight Performance Evaluation of DGPS/INS

  • Park, Ji-Hee;Shin, Dong-Ho
    • Journal of Positioning, Navigation, and Timing
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    • v.1 no.1
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    • pp.45-49
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    • 2012
  • The flight performance evaluation of navigation system is very significant because the reliability of navigation data directly affect the safety of aircraft. Especially, the high-level navigation system such as DGPS/INS, need more precise flight performance evaluation method. The performance analysis is evaluated by comparing between the navigation system in aircraft and reference trajectory which is more precise than navigation system in aircraft. In order to verify DGPS/INS performance of m-level, the GPS receiver, which is capable post-processed Carrier-phase Differential GPS(CDGPS) method of cm-level, have to be used as reference system. The DGPS/INS is estimated the Center of Gravity (CG) point of aircraft to offer precise performance while the reference system is output the position of GPS antenna which is mounted on the outside of aircraft. Therefore, in order to more precise performance evaluation, it needs to compensate the lever arm and coordinates transformation. This paper use quaternion and Direct Cosine Matrix(DCM) methods as coordinate transformation matrix in lever arm compensation of CDGPS reference trajectory. And it compares NED errors of DCM and quaternion transformation in lever arm of reference trajectory via DGPS/INS result.

Design of control systems by a linear fractional transformation (선형분수변환을 이용한 제어계설계)

  • ;古田 勝久
    • Journal of Advanced Marine Engineering and Technology
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    • v.13 no.2
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    • pp.78-88
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    • 1989
  • The fundamental objective of this paper has been to develop a means for incoporating the concept of the linear fractional transformation more generally and easily into multivariable feedback design procedure. When we design a continuous system, generally, we are constrained by design methods which arise specifically for the system. Also, in the design of descrete systems, it is the same concept. But the approach developed in this paper is very flexible in the view that in spite of being the continuous or discrete, the design can be done using a well known design method in both cases. That is, when we design a contnuous system or discrete system, the design can be done by a standard design method of continuous systmes or discrete ones, depending on the choice of the linear fractional transformation. Therefore, it is noted that this concept has broken the unflexibility of the conventional design rules for multivariable control system. In essence, the concept shows that if a given system is controllable, some desirable design, for examples, pole assignment within prespecified region, optimal controllers with poles within prespecified region etc., could be done easily by transforming a desirable region into a standard region, such as the complex left-half plane or the unit disk, by the chosen linear fractional transformation, and then by designing the transformed system using the well known standard results.

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Oxidative Transformation of Triclosan by Laccase (Laccase를 이용한 Triclosan의 처리)

  • Kim, Young-Jin
    • Journal of Environmental Health Sciences
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    • v.31 no.1
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    • pp.61-65
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    • 2005
  • The oxidative transformation of triclosan with laccase from Trametes versicolor was conducted in a closed, temperature controlled system containing phosphate buffer for pH control. The optimum pH for triclosan transformation showed about 5. Despite the observation that elevated temperatures tended to inactivate the enzyme, increased transformation of triclosan was observed up to $50^{\circ}C$. Of the mediators studied, ABTS was most successful at enhancing triclosan transformation. About 80% of the toxicity of the initial mixture was reduced after the enzymatic treatment. In the presence of 1.0 mM of anions such as sulfite, sulfide, and cyanide, triclosan transformation was greatly inhibited. Chloride and fluoride ions exhibited inhibition of triclosan transformation at 25 mM. Ferric ion substantially inhibited triclosan transformation at 1.0 mM.

Development of Query Transformation Method by Cost Optimization

  • Altayeva, Aigerim Bakatkaliyevna;Yoon, Youngmi;Cho, Young Im
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.16 no.1
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    • pp.36-43
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    • 2016
  • The transformation time among queries in the database management system (DBMS) is responsible for the execution time of users' queries, because a conventional DBMS does not consider the transformation cost when queries are transformed for execution. To reduce the transformation time (cost reduction) during execution, we propose an optimal query transformation method by exploring queries from a cost-based point of view. This cost-based point of view means considering the cost whenever queries are transformed for execution. Toward that end, we explore and compare set off heuristic, linear, and exhaustive cost-based transformations. Further, we describe practical methods of cost-based transformation integration and some query transformation problems. Our results show that, some cost-based transformations significantly improve query execution time. For instance, linear and heuristic transformed queries work 43% and 74% better than exhaustive queries.

Transformation of Bacillus Subtilis by Streptomyces bobili R-Plasmid DNA (Streptomyces bobili의 R-Plasmid. DNA에 의한 Bacillus subtilis의 Transformation)

  • 김상달;도재호
    • Microbiology and Biotechnology Letters
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    • v.11 no.3
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    • pp.163-168
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    • 1983
  • The penicillin resistant plasmid DNA was prepared from Streptomyces bobili YS-40, producing penicillinase, by the phenol extraction method and introduced into Bocillus subtilis IAM 12118 by the transformation procedure of Mahler method. The optimal pH and temperature on the transformation was 7.0, 3$0^{\circ}C$ respectively. Above 20 minutes contact of plasmid DNA and recipient cell was shown the high transformation frequency. The transformant of penicillin resistance was proportionally increased as increase of the DNA concentration. The addition of lysine in transformation system increased the transformation frequency about 6-fold and the addition of the chloramphenicol did not affect the transformation frequency.

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A Systematic Formulation for Dynamics of Flexible Multibody Systems (탄성 다물체계의 체계적인 동역학적 해석)

  • 이병훈;유완석
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.17 no.10
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    • pp.2483-2490
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    • 1993
  • This paper presents a systematic formulation for the kinematic and dynamic analysis of flexible multibody systems. The system equations of motion are derived in terms of relative and elastic coordinates using velocity transformation technique. The position transformation equations that relate the relative and elastic coordinates to the Cartesian coordinates for the two contiguous flexible bodies are derived. The velocity transformation matrix is derived systematically corresponding to the type of kinematic joints connecting the bodies and system path matrix. This matrix is employed to represent the equations of motion in relative coordinate space. Two examples are taken to test the method developed here.

An acceleration transformation algorithm for strapdown inertial navigation system (스트랩다운 관성항법시스템의 가속도 변환 알고리즘)

  • 김광진;김정환;백양식
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.1671-1674
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    • 1997
  • In tihs paper, an acceleratiion transformation algorithm which compensates the sculling error is proposed for strapdown inertial navigation system. The algorithm utilize the corss-product of the acceleromenter outputs and gyro outputs to keep the accuracy of the vehicle velocity under high frequency dynamic motion. From the error analysis of the algorithm, it is shown that the magnitude of estimation error is reduced by four orders.

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