• Nutrition Research and Practice
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• v.11 no.6
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• pp.470-478
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• 2017

BACKGROUND/OBJECTIVE: Orostachys japonicus A. Berger (Crassulaceae) has been used in traditional herbal medicines in Korea and other Asian countries to treat various diseases, including liver disorders. In the present study, the anti-fibrotic effects of O. japonicus extract (OJE) in cellular and experimental hepatofibrotic rat models were investigated. MATERIALS/METHODS: An in vitro hepatic stellate cells (HSCs) system was used to estimate cell viability, cell cycle and apoptosis by MTT assay, flow cytometry, and Annexin V-FITC/PI staining techniques, respectively. In addition, thioacetamide (TAA)-induced liver fibrosis was established in Sprague Dawley rats. Briefly, animals were divided into five groups (n = 8): Control, TAA, OJE 10 (TAA with OJE 10 mg/kg), OJE 100 (TAA with OJE 100 mg/kg) and silymarin (TAA with Silymarin 50 mg/kg). Fibrosis was induced by treatment with TAA (200 mg/kg, i.p.) twice per week for 13 weeks, while OJE and silymarin were administered orally two times per week from week 7 to 13. The fibrotic related gene expression serum biomarkers glutathione and hydroxyproline were estimated by RT-PCR and spectrophotometry, respectively, using commercial kits. RESULTS: OJE (0.5 and 0.1 mg/ mL) and silymarin (0.05 mg/mL) treatment significantly (P < 0.01 and P < 0.001) induced apoptosis (16.95% and 27.48% for OJE and 25.87% for silymarin, respectively) in HSC-T6 cells when compared with the control group (9.09%). Further, rat primary HSCs showed changes in morphology in response to OJE 0.1 mg/mL treatment. In in vivo studies, OJE (10 and 100 mg/kg) treatment significantly ameliorated TAA-induced alterations in levels of serum biomarkers, fibrotic related gene expression, glutathione, and hydroxyproline (P < 0.05-P < 0.001) and rescued the histopathological changes. CONCLUSIONS: OJE can be developed as a potential agent for the treatment of hepatofibrosis.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.2
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• pp.153-165
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• 1997

In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%${\sim}$-3.8%. The Pearson correlation coefficient between readers was 0.79. Between the manual and sperm morphology analyzer, the same findings were reported. In this experiments the slides were stained by two different methods, PAP and Diff-Quik staining methods. The limits of agreement were 7.2%${\sim}$-5.7% and 6.0%${\sim}$-6.3%, respectively. The Pearson correlation coefficients ware 0.76 and 0.91, respectively. The limits of agreement was tighter below 20% normal forms. In the experiments of repeatability, 52 cells stained by PAP and Diff-Quik staining methods were analyzed three times in succession. Estimating pairwise agreement, the kappa statistic for the pairs were 0.76, 0.81, 0.86, and 0.75, 0.88, 0.88 respectively. In this study it was shown that there was good agreement between manual and computerized assessment of normal and abnormal cells. The repeatability and agreement per slide of computerized sperm morphology analyzer was excellent. The computer's ability to classify normal morphology per slide is promising. Based on results obtained, this system can be of clinical value both in andrology laboratories and IVF units.

• Journal of the Korea Society of Computer and Information
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• v.11 no.3
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• pp.117-124
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• 2006

In this paper, we propose the optimal migration path searching method including path adjustment and reassignment techniques for an efficient migration of mobile agent which has an autonomous ability for some task processing. In traditional agent system, if the various and large quantify of task processing were required from the users, it could be derive excessive network traffic and overload due to increasing the size of agent. And also, if an agent migrates from node to node according to routing schedules specified by the user at which the most of network traffic occurs, it could not actively cope with particular situations such as communication loss and node obstacles and required much cost for node traversal Therefore. this paper presents the migration method of agent which can try to adjust and reassign path to the destination automatically through the optimal path search using by network traffic perception in stead of the passive routing schedule by the user. An optimal migration path searching and adjustment methods ensure the migration reliability on distributed nodes and reduce the traversal task processing time of mobile agent by avoiding network traffic paths and node obstacles.

• Korean Journal of Remote Sensing
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• v.37 no.5_1
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• pp.1083-1094
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• 2021

In order to monitor nuclear activity in inaccessible areas, it is necessary to establish a methodology to analyze changesin nuclear activity-related objects using high-resolution satellite images. However, traditional object detection and change detection techniques using satellite images have difficulties in applying detection results to various fields because effects of seasons and weather at the time of image acquisition. Therefore, in this paper, an object of interest was detected in a satellite image using a deep learning model, and object changes in the satellite image were analyzed based on object detection results. An initial training of the deep learning model was performed using an open dataset for object detection, and additional training dataset for the region of interest were generated and applied to transfer learning. After detecting objects by multitemporal and multisensory satellite images, we tried to detect changes in objects in the images by using them. In the experiments, it was confirmed that the object detection results of various satellite images can be directly used for change detection for nuclear activity-related monitoring in inaccessible areas.

• Journal of Conservation Science
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• v.36 no.6
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• pp.562-577
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• 2020

In this study, to conserve the clay seated Vairocana Buddha triad in Seonunsa temple, Gochang (Treasure 1752), the primary materials employed for creating the clay Buddha statues were scientifically analyzed. By analyzing the soil layer constituting the Buddha statues, it is observed that the said layer comprises sand having particle size greater than that of medium sand and soil having particle size smaller than that of fine sand, which were used in a ratio of 7:3, along with the bast fibers of paper mulberry (Broussonetia kazinoki). Hence, the aforementioned soil layer is composed of a mixture of sand and weathered soil, along with bast fibers to prevent scattering. By analyzing the tree species, it is found that the wooden materials constituting the bottom board and the interior of Buddha's sleeves of the Amitabha Buddha statue, Vairocana Buddha statue, and Medicine Buddha statue are hard pines (Pinus spp.). Additionally, three layers are found in the cross section of the gold layer. Furthermore, each of the hair sections of the Buddha statues is composed of earthy materials such as quartz, albite, microcline, mica, and magnetite, and the hair surface was painted by incorporating black materials containing magnetite.

• Korean Journal of Heritage: History & Science
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• v.46 no.1
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• pp.140-159
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• 2013

The wooden stupa of Hwangyongsa temple - which was designed and constructed by master craftsman Abigi of the Baekje Dynasty during the Silla Dynasty between the 12th year of Queen Seondeok (AD 643) and AD 646 - can be regarded as a typical Korean wooden stupa. However, it was destroyed by fire during an invasion by the Mongolians in AD 1238 during the Koryo Dynasty. In spite of progress in research for the restoration of the wooden stupa, there are some remains to be re-considered in regard to ruins, relics and literature previously discovered. In particular, research conducted on frame construction and structure can be considered an important basis for restoration. However, previous studies seem to have not attempted to establish structural and formative characteristics of the wooden stupa based on the indigenous techniques and styles of traditional Korea, but general characteristics of the wooden stupa biased toward cultural interchange with neighbouring countries. As such, in this study the frame construction and structure of the wooden stupa of Hwangyongsa temple were analysed and considered in detail based on the re-interpretation of literature which can be a clue about the structure of the stupa, related ruins of the 7th century period, and previous studies. As a result, this paper supposed and presented a model picture for the plane arrangement of pillars, structural methods for Dwibburi (뒤뿌리) of Haangjae (하앙재), Shimju (심주), and other key areas. The author hopes that this study contributes substantially to research on the wooden stupa of ancient Korea and to the establishment of construction and structural methods.

• Korean Journal of Heritage: History & Science
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• v.44 no.3
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• pp.4-17
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• 2011

The Baekje's cotton fabrics were excavated from 'Neungsan-ri temple site in Buyeo' (September 1999-April 2000) and stored at Buyeo National Museum after conservation treatment. In this study, it carred out non-destructive, chemical and morphological analysis for fiber identification, also considered on influx, features and difference between the 'Baek-cheop-po(Three kingdom period's cotton fiber)' and 'Mok-myeon(imported by Munikjeom, late Korea dynasty)'. As a result, the fiber proved cellulose fiber through analytical researches like color reaction, FT-IR(chemical analysis). It was also confirmed lumens, typical dimensional structure(morphological analysis) as an features of cotton fiber. The fiber was the first evidence in ancient Korea's cotton. But it can not prove that whether weaving were made in Baekje's area. However there were documentation that people in Beakje make cloth to silk fabric from 'Mahan period'. We can suppose that they have had an old weaving techniques. This study has a great historical, academic values as the only evidence for the hypothesis of a weaving technique of the Baekje's cotton. Through comparison to each region's ancient cottons, we can investigate the species of Baekje's cotton and ancient Korea cotton's influx.

• Korean Journal of Heritage: History & Science
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• v.50 no.3
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• pp.30-43
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• 2017

The purpose of this study is to improve the usefulness of photogrammetry in the field of cultural heritage recording concentrated on laser scanning. Two measurement methods(laser scanning, photogrammetry) were compared in terms of accuracy and reality for the Rock-carved Standing Buddha in Singyeong-ri, Hongseong. With regard to accuracy, the distances of major points by both shape information and between the two shape information were compared. Only a deviation of about 1mm was found in the distance measurement of the major points by both shape information. In particular, the average distance between two shape information identified through aligning was only about 0.01mm. Also, the absolute deviation within about 2mm accounted for 70% of the total, and the absolute deviation within about 3.5mm was found to be 95.4% of the total. These values showed very high similarity between laser scanning and photogrammetry-based shape information. In respect of reality, the carved depth, texture, and patterns were compared. As a result of comparing four cross-sectional shapes, only slight differences were found in the shape information of both measurement techniques and similar shapes were identified. The overall texture of both shape information was also similar. However, the detailed shape based on the photogrammetry with decimation is realized with a smoother texture than the original and laser scanning. In particular, Photogrammetry also realistically expressed the various ornaments carved in the Rock-carved Buddha and the patterns with shallow depths were comparatively detailed.

• Korean Journal of Heritage: History & Science
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• v.47 no.1
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• pp.46-65
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• 2014

This study, under the title of Daeungbojeon Hall of Bulyeongsa Temple, Uljin and the architectural technique of the architect, aimed to make a comprehensive speculation on Daeungbojeon Hall of Bulyeongsa Temple whose accurate construction year was confirmed in 1725. While existing studies dealt with it separately between construction and Buddhist art history this study attempted to sort out it in one perspective by means of comparison with compatible objects in the area. The results are as follows. 1st, by means of comparison of wall painting of Daeungbojeon Hall of Bulyeongsa Temple and other wall paintings of Gyeongsang-do Province, it was estimated to be created before and after 1725, the founding year of the building. 2nd, the stylobate of Daeungbojeon Hall is the only and unique case that Guibu was supported by the bottom. Such stylobate was estimated to be built in the early period of Goryeo stylobate of PostLintel Construction which was more simplified than that of the traditional unified Silla period considering specific techniques. Lastly, by means of comparison of the architectural technique of Daeungbojeon Hall of Bulyeongsa Temple with other temples in Gyeongsang-do Province, the characteristics of the building were found. In particular, the same architectural technique was confirmed by direct comparison of style with Yeongsanjeon Hall of Tongdosa Temple in 1714 which was constructed by the same architect.

• Korean Journal of Heritage: History & Science
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• v.39
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• pp.191-217
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• 2006

The chief mourners do their best to carry the dead and various things related to him in the departure of a funeral procession. "Sejongsillok" and "Gukjooryeui" had already written the national funeral supplies for carriage in detail in the early of Joseon period. But they were the standard book of national ceremony. The Ilbang of Gukjangdogam made the national funeral supplies for carriage in the late of Joseon; Daeyeo, Gyeonyeo, Yoyeo, Chaeyeo, Gaja etc. "Gukjangdogamuigye" explained these in detail; size, materials, made-place etc. I used "Gukjangdogamuigye" published in the late of Joseon to explain the national funeral supplies for carriage. Because Kingdom's ceremony in Joseon was representative to the country, it couldn't depend on an individual taste. King was the symbol of a nation and the star tin point of the political power at that time. So its ceremony was to show people the representative example as a national ceremony. The national funeral supplies for carriage carried the dead of King and various things related to him. Gukjangdogam managed them carefully. People thought that the mean of these things was equal to King's authority at that time. So these weren't the mere national funeral supplies for carriage, the symbolic instruments equal to King.