Clinical and Experimental Reproductive Medicine
- Volume 24 Issue 2
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- Pages.153-165
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- 1997
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
A Study on the Morphological Analysis of Sperm
정자의 형태학적 특성 분석에 관한 연구
- Paick, Jae-Seung (Department of Urology, Seoul National University College of Medicine) ;
- Jeon, Seong-Soo (Department of Urology, Seoul National University College of Medicine) ;
- Kim, Soo-Woong (Department of Urology, Seoul National University College of Medicine) ;
- Yi, Won-Jin (Department of Biomedical Engineering, Seoul National University College of Medicine) ;
- Park, Kwang-Suk (Department of Biomedical Engineering, Seoul National University College of Medicine)
- 백재승 (서울대학교 의과대학 비뇨기과학교실) ;
- 전성수 (서울대학교 의과대학 비뇨기과학교실) ;
- 김수웅 (서울대학교 의과대학 비뇨기과학교실) ;
- 이원진 (서울대학교 의과대학 의공학교실) ;
- 박광석 (서울대학교 의과대학 의공학교실)
- Published : 1997.08.30
Abstract
In male reproducible health, fertility and IVF (in-vitro fertilization), semen analysis has been most important. Semen analysis can be divided into concentration, motional and morphological analysis of sperm. The existing method which was developed earlier to analyze semen concentrated on the sperm motility analysis. To provide more useful and precise solutions for clinical problems such as infertility, semen analysis must include sperm morphological analysis. But the traditional tools for semen analysis are subjective, imprecise, inaccurate, difficult to standardize, and difficult to reproduce. Therefore, with the help of development of microcomputers and image processing techniques, we developed a new sperm morphology analyzer to overcome these problems. In this study the agreement on percent normal morphology was studied between different observers and a computerized sperm morphology analyzer on a slide-by-slide basis using strict criteria. Slides from 30 different patients from the SNUH andrology laboratory were selected randomly. Microscopic fields and sperm cells were chosen randomly and percent normal morphology was recorded. The ability of sperm morphology analyzer to repeat the same reading for normal and abnormal cells was studied. The results showed that there was no significant bias between two experienced observers. The limits of agreement were 4.1%