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QTL Identification for Slow Wilting and High Moisture Contents in Soybean (Glycine max [L.]) and Arduino-Based High-Throughput Phenotyping for Drought Tolerance

  • Hakyung Kwon;Jae Ah Choi;Moon Young Kim;Suk-Ha Lee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.25-25
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    • 2022
  • Drought becomes frequent and severe because of continuous global warming, leading to a significant loss of crop yield. In soybean (Glycine max [L.]), most of quantitative trait loci (QTLs) analyses for drought tolerance have conducted by investigating yield changes under water-restricted conditions at the reproductive stages. More recently, the necessity of QTL studies to use physiological indices responding to drought at the early growth stages besides the reproductive ones has arisen due to the unpredictable and prevalent occurrence of drought throughout the soybean growing season. In this study, we thus identified QTLs conferring wilting scores and moisture contents of soybean subjected to drought stress in the early vegetative stage using an recombinant inbred line (RIL) population derived from a cross between Taekwang (drought-sensitive) and SS2-2 (drought-tolerant). For the two traits, the same major QTL was located on chromosome 10, accounting for up to 11.5% of phenotypic variance explained with LOD score of 12.5. This QTL overlaps with a reported QTL for the limited transpiration trait in soybean and harbors an ortholog of the Arabidopsis ABA and drought-induced RING-D UF1117 gene. Meanwhile, one of important features of plant drought tolerance is their ability to limit transpiration rates under high vapor pressure deficiency in response to mitigate water loss. However, monitoring their transpiration rates is time-consuming and laborious. Therefore, only a few population-level studies regarding transpiration rates under the drought condition have been reported so far. Via employing an Arduino-based platform, for the reasons addressed, we are measuring and recording total pot weights of soybean plants every hour from the 1st day after water restriction to the days when the half of the RILs exhibited permanent tissue damage in at least one trifoliate. Gradual decrease in moisture of soil in pots as time passes refers increase in the severity of drought stress. By tracking changes in the total pot weights of soybean plants, we will infer transpiration rates of the mapping parents and their RILs according to different levels of VPD and drought stress. The profile of transpiration rates from different levels of severity in the stresses facilitates a better understanding of relationship between transpiration-related features, such as limited maximum transpiration rates, to water saving performances, as well as those to other drought-responsive phenotypes. Our findings will provide primary insights on drought tolerance mechanisms in soybean and useful resources for improvement of soybean varieties tolerant to drought stress.

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Utilization of Weather, Satellite and Drone Data to Detect Rice Blast Disease and Track its Propagation (벼 도열병 발생 탐지 및 확산 모니터링을 위한 기상자료, 위성영상, 드론영상의 공동 활용)

  • Jae-Hyun Ryu;Hoyong Ahn;Kyung-Do Lee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.245-257
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    • 2023
  • The representative crop in the Republic of Korea, rice, is cultivated over extensive areas every year, which resulting in reduced resistance to pests and diseases. One of the major rice diseases, rice blast disease, can lead to a significant decrease in yields when it occurs on a large scale, necessitating early detection and effective control of rice blast disease. Drone-based crop monitoring techniques are valuable for detecting abnormal growth, but frequent image capture for potential rice blast disease occurrences can consume significant labor and resources. The purpose of this study is to early detect rice blast disease using remote sensing data, such as drone and satellite images, along with weather data. Satellite images was helpful in identifying rice cultivation fields. Effective detection of paddy fields was achieved by utilizing vegetation and water indices. Subsequently, air temperature, relative humidity, and number of rainy days were used to calculate the risk of rice blast disease occurrence. An increase in the risk of disease occurrence implies a higher likelihood of disease development, and drone measurements perform at this time. Spectral reflectance changes in the red and near-infrared wavelength regions were observed at the locations where rice blast disease occurred. Clusters with low vegetation index values were observed at locations where rice blast disease occurred, and the time series data for drone images allowed for tracking the spread of the disease from these points. Finally, drone images captured before harvesting was used to generate spatial information on the incidence of rice blast disease in each field.

Development of a Method for Tracking Sandbar Formation by Weir-Gate Opening Using Multispectral Satellite Imagery in the Geumgang River, South Korea (금강에서 다분광 위성영상을 이용한 보 운영에 따른 모래톱 형성 추적 방법의 개발)

  • Cheolho Lee;Kang-Hyun Cho
    • Ecology and Resilient Infrastructure
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    • v.10 no.4
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    • pp.135-142
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    • 2023
  • A various technology of remote sensing and image analysis are applied to study landscape changes and their influencing factors in stream corridors. We developed a method to detect landscape changes over time by calculating the optical index using multispectral images taken from satellites at various time points, calculating the threshold to delineate the boundaries of water bodies, and creating binarized maps into land and water areas. This method was applied to the upstream reach of the weirs in the Geumgang River to track changes in the sandbar formed by the opening of the weir gate. First, we collected multispectral images with a resolution of 10 m × 10 m taken from the Sentinel-2 satellite at various times before and after the opening of the dam in the Geumgang River. The normalized difference water index (NDWI) was calculated using the green light and near-infrared bands from the collected images. The Otsu's threshold of NDWI calculated to delineate the boundary of the water body ranged from -0.0573 to 0.1367. The boundary of the water area determined by remote sensing matched the boundary in the actual image. A map binarized into water and land areas was created using NDWI and the Otsu's threshold. According to these results of the developed method, it was estimated that a total of 379.7 ha of new sandbar was formed by opening the three weir floodgates from 2017 to 2021 in the longitudinal range from Baekje Weir to Daecheong Dam on the Geumgang River. The landscape detection method developed in this study is evaluated as a useful method that can obtain objective results with few resources over a wide spatial and temporal range.

A Model for Constructing Learner Data in AI-based Mathematical Digital Textbooks for Individual Customized Learning (개별 맞춤형 학습을 위한 인공지능(AI) 기반 수학 디지털교과서의 학습자 데이터 구축 모델)

  • Lee, Hwayoung
    • Education of Primary School Mathematics
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    • v.26 no.4
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    • pp.333-348
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    • 2023
  • Clear analysis and diagnosis of various characteristic factors of individual students is the most important in order to realize individual customized teaching and learning, which is considered the most essential function of math artificial intelligence-based digital textbooks. In this study, analysis factors and tools for individual customized learning diagnosis and construction models for data collection and analysis were derived from mathematical AI digital textbooks. To this end, according to the Ministry of Education's recent plan to apply AI digital textbooks, the demand for AI digital textbooks in mathematics, personalized learning and prior research on data for it, and factors for learner analysis in mathematics digital platforms were reviewed. As a result of the study, the researcher summarized the factors for learning analysis as factors for learning readiness, process and performance, achievement, weakness, and propensity analysis as factors for learning duration, problem solving time, concentration, math learning habits, and emotional analysis as factors for confidence, interest, anxiety, learning motivation, value perception, and attitude analysis as factors for learning analysis. In addition, the researcher proposed noon data on the problem, learning progress rate, screen recording data on student activities, event data, eye tracking device, and self-response questionnaires as data collection tools for these factors. Finally, a data collection model was proposed that time-series these factors before, during, and after learning.

Real-time Body Surface Motion Tracking using the Couch Based Computer-controlled Motion Phantom (CBMP) and Ultrasonic Sensor: A Feasibility Study (CBMP (Couch Based Computer-Controlled Motion Phantom)와 초음파센서에 기반한 실시간 체표면 추적 시스템 개발: 타당성 연구)

  • Lee, Suk;Yang, Dae-Sik;Park, Young-Je;Shin, Dong-Ho;Huh, Hyun-Do;Lee, Sang-Hoon;Cho, Sam-Ju;Lim, Sang-Wook;Jang, Ji-Sun;Cho, Kwang-Hwan;Shin, Hun-Joo;Kim, Chul-Yong
    • Progress in Medical Physics
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    • v.18 no.1
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    • pp.27-34
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    • 2007
  • Respiration sating radiotherapy technique developed In consideration of the movement of body surface and Internal organs during respiration, is categorized into the method of analyzing the respiratory volume for data processing and that of keeping track of fiducial landmark or dermatologic markers based on radiography. However, since these methods require high-priced equipments for treatment and are used for the specific radiotherapy. Therefore, we should develop new essential method whilst ruling out the possible problems. This study alms to obtain body surface motion by using the couch based computer-controlled motion phantom (CBMP) and US sensor, and to develop respiration gating techniques that can adjust patients' beds by using opposite values of the data obtained. The CBMP made to measure body surface motion is composed of a BS II microprocessor, sensor, host computer and stopping motor etc. And the program to control and operate It was developed. After the CBMP was adjusted by entering random movement data, and the phantom movements were acquired using the sensors, the two data were compared and analyzed. And then, after the movements by respiration were acquired by using a rabbit, the real-time respiration gating techniques were drawn by operating the phantom with the opposite values of the data. The result of analysing the acquisition-correction delay time for the data value shows that the data value coincided within 1% and that the acquistition-correction delay time was obtained real-time $(2.34{\times}10^{-4}sec)$. And the movement was the maximum movement was 6 mm In Z direction, In which the respiratory cycle was 2.9 seconds. This study successfully confirms the clinical application possibility of respiration gating techniques by using a CBWP and sensor.

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A Study on People Counting in Public Metro Service using Hybrid CNN-LSTM Algorithm (Hybrid CNN-LSTM 알고리즘을 활용한 도시철도 내 피플 카운팅 연구)

  • Choi, Ji-Hye;Kim, Min-Seung;Lee, Chan-Ho;Choi, Jung-Hwan;Lee, Jeong-Hee;Sung, Tae-Eung
    • Journal of Intelligence and Information Systems
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    • v.26 no.2
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    • pp.131-145
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    • 2020
  • In line with the trend of industrial innovation, IoT technology utilized in a variety of fields is emerging as a key element in creation of new business models and the provision of user-friendly services through the combination of big data. The accumulated data from devices with the Internet-of-Things (IoT) is being used in many ways to build a convenience-based smart system as it can provide customized intelligent systems through user environment and pattern analysis. Recently, it has been applied to innovation in the public domain and has been using it for smart city and smart transportation, such as solving traffic and crime problems using CCTV. In particular, it is necessary to comprehensively consider the easiness of securing real-time service data and the stability of security when planning underground services or establishing movement amount control information system to enhance citizens' or commuters' convenience in circumstances with the congestion of public transportation such as subways, urban railways, etc. However, previous studies that utilize image data have limitations in reducing the performance of object detection under private issue and abnormal conditions. The IoT device-based sensor data used in this study is free from private issue because it does not require identification for individuals, and can be effectively utilized to build intelligent public services for unspecified people. Especially, sensor data stored by the IoT device need not be identified to an individual, and can be effectively utilized for constructing intelligent public services for many and unspecified people as data free form private issue. We utilize the IoT-based infrared sensor devices for an intelligent pedestrian tracking system in metro service which many people use on a daily basis and temperature data measured by sensors are therein transmitted in real time. The experimental environment for collecting data detected in real time from sensors was established for the equally-spaced midpoints of 4×4 upper parts in the ceiling of subway entrances where the actual movement amount of passengers is high, and it measured the temperature change for objects entering and leaving the detection spots. The measured data have gone through a preprocessing in which the reference values for 16 different areas are set and the difference values between the temperatures in 16 distinct areas and their reference values per unit of time are calculated. This corresponds to the methodology that maximizes movement within the detection area. In addition, the size of the data was increased by 10 times in order to more sensitively reflect the difference in temperature by area. For example, if the temperature data collected from the sensor at a given time were 28.5℃, the data analysis was conducted by changing the value to 285. As above, the data collected from sensors have the characteristics of time series data and image data with 4×4 resolution. Reflecting the characteristics of the measured, preprocessed data, we finally propose a hybrid algorithm that combines CNN in superior performance for image classification and LSTM, especially suitable for analyzing time series data, as referred to CNN-LSTM (Convolutional Neural Network-Long Short Term Memory). In the study, the CNN-LSTM algorithm is used to predict the number of passing persons in one of 4×4 detection areas. We verified the validation of the proposed model by taking performance comparison with other artificial intelligence algorithms such as Multi-Layer Perceptron (MLP), Long Short Term Memory (LSTM) and RNN-LSTM (Recurrent Neural Network-Long Short Term Memory). As a result of the experiment, proposed CNN-LSTM hybrid model compared to MLP, LSTM and RNN-LSTM has the best predictive performance. By utilizing the proposed devices and models, it is expected various metro services will be provided with no illegal issue about the personal information such as real-time monitoring of public transport facilities and emergency situation response services on the basis of congestion. However, the data have been collected by selecting one side of the entrances as the subject of analysis, and the data collected for a short period of time have been applied to the prediction. There exists the limitation that the verification of application in other environments needs to be carried out. In the future, it is expected that more reliability will be provided for the proposed model if experimental data is sufficiently collected in various environments or if learning data is further configured by measuring data in other sensors.

GPR Development for Landmine Detection (지뢰탐지를 위한 GPR 시스템의 개발)

  • Sato, Motoyuki;Fujiwara, Jun;Feng, Xuan;Zhou, Zheng-Shu;Kobayashi, Takao
    • Geophysics and Geophysical Exploration
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    • v.8 no.4
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    • pp.270-279
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    • 2005
  • Under the research project supported by Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT), we have conducted the development of GPR systems for landmine detection. Until 2005, we have finished development of two prototype GPR systems, namely ALIS (Advanced Landmine Imaging System) and SAR-GPR (Synthetic Aperture Radar-Ground Penetrating Radar). ALIS is a novel landmine detection sensor system combined with a metal detector and GPR. This is a hand-held equipment, which has a sensor position tracking system, and can visualize the sensor output in real time. In order to achieve the sensor tracking system, ALIS needs only one CCD camera attached on the sensor handle. The CCD image is superimposed with the GPR and metal detector signal, and the detection and identification of buried targets is quite easy and reliable. Field evaluation test of ALIS was conducted in December 2004 in Afghanistan, and we demonstrated that it can detect buried antipersonnel landmines, and can also discriminate metal fragments from landmines. SAR-GPR (Synthetic Aperture Radar-Ground Penetrating Radar) is a machine mounted sensor system composed of B GPR and a metal detector. The GPR employs an array antenna for advanced signal processing for better subsurface imaging. SAR-GPR combined with synthetic aperture radar algorithm, can suppress clutter and can image buried objects in strongly inhomogeneous material. SAR-GPR is a stepped frequency radar system, whose RF component is a newly developed compact vector network analyzers. The size of the system is 30cm x 30cm x 30 cm, composed from six Vivaldi antennas and three vector network analyzers. The weight of the system is 17 kg, and it can be mounted on a robotic arm on a small unmanned vehicle. The field test of this system was carried out in March 2005 in Japan.

Issue tracking and voting rate prediction for 19th Korean president election candidates (댓글 분석을 통한 19대 한국 대선 후보 이슈 파악 및 득표율 예측)

  • Seo, Dae-Ho;Kim, Ji-Ho;Kim, Chang-Ki
    • Journal of Intelligence and Information Systems
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    • v.24 no.3
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    • pp.199-219
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    • 2018
  • With the everyday use of the Internet and the spread of various smart devices, users have been able to communicate in real time and the existing communication style has changed. Due to the change of the information subject by the Internet, data became more massive and caused the very large information called big data. These Big Data are seen as a new opportunity to understand social issues. In particular, text mining explores patterns using unstructured text data to find meaningful information. Since text data exists in various places such as newspaper, book, and web, the amount of data is very diverse and large, so it is suitable for understanding social reality. In recent years, there has been an increasing number of attempts to analyze texts from web such as SNS and blogs where the public can communicate freely. It is recognized as a useful method to grasp public opinion immediately so it can be used for political, social and cultural issue research. Text mining has received much attention in order to investigate the public's reputation for candidates, and to predict the voting rate instead of the polling. This is because many people question the credibility of the survey. Also, People tend to refuse or reveal their real intention when they are asked to respond to the poll. This study collected comments from the largest Internet portal site in Korea and conducted research on the 19th Korean presidential election in 2017. We collected 226,447 comments from April 29, 2017 to May 7, 2017, which includes the prohibition period of public opinion polls just prior to the presidential election day. We analyzed frequencies, associative emotional words, topic emotions, and candidate voting rates. By frequency analysis, we identified the words that are the most important issues per day. Particularly, according to the result of the presidential debate, it was seen that the candidate who became an issue was located at the top of the frequency analysis. By the analysis of associative emotional words, we were able to identify issues most relevant to each candidate. The topic emotion analysis was used to identify each candidate's topic and to express the emotions of the public on the topics. Finally, we estimated the voting rate by combining the volume of comments and sentiment score. By doing above, we explored the issues for each candidate and predicted the voting rate. The analysis showed that news comments is an effective tool for tracking the issue of presidential candidates and for predicting the voting rate. Particularly, this study showed issues per day and quantitative index for sentiment. Also it predicted voting rate for each candidate and precisely matched the ranking of the top five candidates. Each candidate will be able to objectively grasp public opinion and reflect it to the election strategy. Candidates can use positive issues more actively on election strategies, and try to correct negative issues. Particularly, candidates should be aware that they can get severe damage to their reputation if they face a moral problem. Voters can objectively look at issues and public opinion about each candidate and make more informed decisions when voting. If they refer to the results of this study before voting, they will be able to see the opinions of the public from the Big Data, and vote for a candidate with a more objective perspective. If the candidates have a campaign with reference to Big Data Analysis, the public will be more active on the web, recognizing that their wants are being reflected. The way of expressing their political views can be done in various web places. This can contribute to the act of political participation by the people.

A Study on the RFID's Application Environment and Application Measure for Security (RFID의 보안업무 적용환경과 적용방안에 관한 연구)

  • Chung, Tae-Hwang
    • Korean Security Journal
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    • no.21
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    • pp.155-175
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    • 2009
  • RFID that provide automatic identification by reading a tag attached to material through radio frequency without direct touch has some specification, such as rapid identification, long distance identification and penetration, so it is being used for distribution, transportation and safety by using the frequency of 125KHz, 134KHz, 13.56MHz, 433.92MHz, 900MHz, and 2.45GHz. Also it is one of main part of Ubiquitous that means connecting to net-work any time and any place they want. RFID is expected to be new growth industry worldwide, so Korean government think it as prospective field and promote research project and exhibition business program to linked with industry effectively. RFID could be used for access control of person and vehicle according to section and for personal certify with password. RFID can provide more confident security than magnetic card, so it could be used to prevent forgery of register card, passport and the others. Active RFID could be used for protecting operation service using it's long distance date transmission by application with positioning system. And RFID's identification and tracking function can provide effective visitor management through visitor's register, personal identification, position check and can control visitor's movement in the secure area without their approval. Also RFID can make possible of the efficient management and prevention of loss of carrying equipments and others. RFID could be applied to copying machine to manager and control it's user, copying quantity and It could provide some function such as observation of copy content, access control of user. RFID tag adhered to small storage device prevent carrying out of item using the position tracking function and control carrying-in and carrying-out of material efficiently. magnetic card and smart card have been doing good job in identification and control of person, but RFID can do above functions. RFID is very useful device but we should consider the prevention of privacy during its application.

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Binder Stiffness Effect on Permanent Deformation and Tensile Strength of Asphalt Concretes (바인더 강성이 아스팔트 콘크리트의 인장강도와 소성변형 특성에 미치는 영향 분석)

  • Kim, Hyun-Hwan;Yoo, Min-Yong;Kim, Jin-Chul;Kim, Kwang-Woo
    • International Journal of Highway Engineering
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    • v.12 no.2
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    • pp.17-23
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    • 2010
  • Since the relatively stiff binder shows a higher tensile strength as well as higher rutting resistance, it is believed that the binder stiffness is an important factor for rutting and tensile strength of asphalt mixtures. The typical tensile property is measured by indirect tensile strength (ITS) test at $25^{\circ}C$ and the rutting resistance is most widely measured by wheel tracking (WT) test at $60^{\circ}C$. The deformation strength ($S_D$) is newly developed property to estimate rut resistance of asphalt concretes at $60^{\circ}C$. The ITS and $S_D$ are very simple to measure by static test techniques, but the WT is measured by repeated loading procedure which requires relatively longer time and more efforts. Since these three properties are highly dependent upon the binder stiffness, it may be possible to estimate one property from another. Therefore, this study investigate the possibility of estimating the rutting characteristics (measured by WT test) by ITS or $S_D$ test, and the ITS by $S_D$. Because of binder stiffness effect, in the WT estimation by ITS, a tendency was observed for the higher ITS mixture to have the lower rut depth, giving $R^2{\fallingdotseq}$0.6, on the average. The ITS estimation by $S_D$ showed $R^2{\fallingdotseq}$0.64, and the WT estimation by SD showed $R^2{\fallingdotseq}$0.84, which is highest correlation among the three. Therefore, it was concluded that there is relatively good possibility of estimating WT result by $S_D$, and even though $R^2$ is somewhat low, there is some correlation between WT and ITS.