• Title/Summary/Keyword: Tracheal secretion

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Effects of Seonbangpaedoktang on secretion of airway mucin and contractility of tracheal smooth muscle (선방패독탕(仙方敗毒湯)이 호흡기 뮤신 분비 및 기관 평활근 긴장도에 미치는 영향)

  • Han, Jae-Kyung;Kim, Yun-Hee;Song, Hyun-Jee
    • The Journal of Pediatrics of Korean Medicine
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    • v.21 no.1
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    • pp.139-154
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    • 2007
  • Objectives : The author intended to investigate Seonbangpaedoktang (SBPT) significantly affect in vivo and in vitro mucin secretion from airway epithelial cells. Methods : In vivo experiment, the author induced hypersecretion of airway mucin, hyperplasia of tracheal goblet cells and the increase in intraepithelial mucosubstances. Effects of orally-administered SBPT during 1 week on in vivo mucin secretion and hyperplasia of tracheal goblet cells were assessed. For in vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled and chased in the presence of SBPT to assess the effect of the agent on 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analysed. Possible cytotoxicity of the agent was assessed by measuring LDH release. Also, the effect of SBPT on contractility of isolated tracheal smooth muscle was investigated. Results : SBPT inhibited hypersecretion of in vivo mucin and inhibited the increase of number of goblet cells ; SBPT did not affect in vitro mucin secretion and the secretion of the other releasable glycoproteins with less molecular weight than mucin from cultured HTSE cells, without significant effect on LDH release; SBPT did not affect Ach-induced contraction of isolated tracheal smooth muscle. Conclusions : SBPT can inihibit hypersecretion of in vivo mucin and the author suggest that the effect SBPT with their components should investigate further.

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The Effect of Chest Physiotherapy on the Amount of Tracheal Secretion and $PaO_2$ (흉부물리요법이 기관 분비물량과 동맥혈 산소분압에 미치는 영향)

  • Jun Seong-Sook;Moon Mi-Jin
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.7 no.3
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    • pp.355-365
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    • 2000
  • The purpose of this research was to find out the effect of chest physiotherapy on the amount of tracheal secretion and $PaO_2$. After changing position of the neurosurgical patients who had tracheostomy cannula, experimental treatments were applied as bellows, manual chest percussion on groupI, chest percussion and manual chest vibration on groupII, chest percussion and mechanical chest vibration on groupIII were done. After these trials, we have analyzed the efficacy of each procedures comparing the group differences in the quantity of tracheal secretion and $PaO_2$. Target samples were sixty patients aged between 20 to 60 who have tracheostomy state and decreased consciousness status that were admitted in NICU of a university hospital from June 1 to August 31, 1999. They assigned randomly into three experimental groups. To compare the effect of each interventions, tracheal secretion quantify was measured and $PaO_2$ was analyzed via arterial blood gas analyzer. The data were analyzed by ANCOVA of 5% significance level using SPSS P/C program. The results were as bellows. 1) The first hypothesis 'There is a difference In the quantify of the secretion among GroupI, GroupII and GroupIII' was accepted.(F=29.27, p=0.00) 2) The second hypothesis 'There is a difference in $PaO_2$ among GroupI, GroupII and GroupIII' was rejected.(F=1.71, p=0.19) From this study results, positional change and manual chest vibration including chest percussion were the most effective treatment to get maximum amount of tracheal secretion and it was confirmed that mechanical chest vibration also made much better effect than sole chest percussion method. Therefore, we concluded that the mechanical or manual chest vibration with chest percussion is more effective respiratory care method than the sole chest percussion.

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Effect of Gamitonggyu-tang on Secretion of Airway Mucin and Contractility of Tracheal Smooth Muscle (가미통규탕(加味通竅湯)이 호흡기 뮤신 분비 및 기관 평활근 긴장도에 미치는 영향)

  • Lee, Nam-Yeol;Han, Jae-Kyung;Kim, Yun-Hee
    • The Journal of Pediatrics of Korean Medicine
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    • v.21 no.3
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    • pp.109-124
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    • 2007
  • Objectives In the present study, the author intended to investigate whether Gamitonggyu-tang (GTT) significantly affects (since the subject is GTT, you need an 's') in vivo and in vitro mucin secretion from airway epithelial cells. Methods In vivo experiment, mice's mucin which is on a hypersecretion of an airway, mice's tracheal goblet cells in hyperplasia and mice's intraepithelial mucosubstances were exposed with SO2 for 3 weeks. Effects of orally-administered GTT for 1 week on in vivo mucin secretion and hyperplasia of tracheal goblet cells were assessed by using enzyme-linked immunosorbent assay (ELISA) and staining goblet cells with alcian blue. In vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of GTT to figure out the effectiveness of 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed.Possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase (LDH) release. Also, the effect of GTT on contractility of isolated tracheal smooth muscle was investigated. Results (1) GTT inhibited hypersecretion of in vivo mucin. However, it did not affect the increase the number of goblet cells (2) GTT significantly increased mucin release from cultured HTSE cells, without significant cytotoxicity (3) GTT chiefly affected the 'mucin' secretion and did not affect the secretion of the other releasable glycoproteins with less molecular weight than mucin (4) GTT did not affect Ach-induced contraction of isolated tracheal smooth muscle.Conclusions This result suggests that GTT can increase mucin secretion during short-term treatment (in vitro) whereas it can inihibit hypersecretion of mucin during long-term treatment (in vivo). The author suggests that the effect GTT with their components should be further investigated and it is valuable to find from oriental medical prescriptions, novel agents which might regulate mucin secretion from airway epithelial cells.

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Effect of Mahwangyunpye-tang on Secretion of Airway Mucin and Tracheal Smooth Muscle (마황윤폐탕(麻黃潤肺湯)이 호흡기 뮤신 분비 및 기관지 평활근에 미치는 영향)

  • Hwang, Ji-Ho;Yang, Su-Young;Byun, Jun-Seop;Park, Yang-Chun
    • The Journal of Internal Korean Medicine
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    • v.28 no.4
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    • pp.797-807
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    • 2007
  • Objectives : The purpose of this study was to investigate whether Mahwangyunpye-tang(MYT) significantly affects mucin secretion from airway epithelial cells. Methods : Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of MYT to assess the effect of the agent on 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed. Effect of MYT on contractility of isolated tracheal smooth muscle was investigated; also investigated was effect of the agent on MUC5AC gene expression in cultured NCI-H292 cells. Possible cytotoxicities of the agent were assessed both by measuring lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results : MYT significantly increased mucin secretion from cultured HTSE cells, without significant cytotoxicity. MYT chiefly affected the 'mucin' secretion. MYT inhibited Acetylcholine-induced contraction of isolated tracheal smooth muscle. MYT did not significantly affect the expression levels of MUC 5AC gene in cultured NCI-H292 cells. Conclusions : Based on the above results, it is suggested that MYT increased mucin secretion from cultured HTSE cells without significant cytotoxicity and inhibited contraction of isolated tracheal smooth muscle.

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Effect of Naenghyo-hwan on Secretion of Airway Mucin and Contractility of Tracheal Smooth Muscle (냉효환(冷哮丸)이 호흡기 뮤신 분비와 기관지 평활근에 미치는 영향)

  • Yoon, Jong-Man;Lee, Yong-Koo;Park, Yang-Chun
    • The Journal of Korean Medicine
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    • v.28 no.2 s.70
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    • pp.54-65
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    • 2007
  • Objectives : In the present study, the author investigated whether Naenghyo-hwan(NHH) significantly affect mucin secretion from airway epithelial cells. Methods : Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of NHH to assess the effect of the agent on 3H-mucin secretion. Total elutionprofiles of control spent media and treatment sample through Sepharose CL-4B column were analysed. Effect of NHH on contractility of isolated tracheal smooth muscle was investigated. Also, effect of the agent on MUC5AC gene expression in cultured NCI-H292cells was investigated. Possible cytotoxicities of the agent were assessed by measuring both lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results : NHH significantly increased mucin secretion from cultured HTSE cells, with significant cytotoxicity. NHH chiefly affected the 'mucin' secretion. NHH inhibited ACh-induced contraction of isolated tracheal smooth muscle. NHH disturbed both the extraction of total RNA from NCI-H292 cells and polymerase chain reaction, nonspecifically. Therefore, in this experiment, theeffect of NHH on the expression levels of MUC 5AC gene in cultured NCI-H292 cells could not be elucidated. Conclusions : The author suggests that the effect of NHH with their components should be further investigated and it is valuable to find, from oriental medical prescriptions, novel agents which might regulate mucin secretion from airway epithelial cells.

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Effects of Cheongjogupye-tang(淸燥救肺湯) and Yieum-jeon(理陰煎) on Secretion of Mucin from Respiratory Epithelial Cells (청조구폐탕(淸燥救肺湯)과 이음전(理陰煎)이 호흡기 접액분비에 미치는 영향)

  • Park, Wan-Yeol;Seo, Un-Kyo
    • The Journal of Internal Korean Medicine
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    • v.29 no.2
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    • pp.318-333
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    • 2008
  • Objectives : In this study, the author tried to examine whether Cheogjogupye-tang (淸燥救肺湯, CGPT) and Yieum-jeon (理陰煎, YEJ) significantly affect in vitro and in vivo mucin secretion, MUC5AC gene expression in airway epithelial cells and contractility of isolated tracheal smooth muscle of rabbit. Materials and Methods : For in vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were chased for 30 minutes in the presence of CGPT and YEJ to assess the effects of the agents on mucin secretion by enzyme-linked immunosorbent assay (ELISA), with removal of oriental herbal medicine extract from each agent-treated sample by centrifuge microfilter. Also, the effects of the agents on TNF-alpha or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Possible cytotoxicities of the agent were assessed by examining both LDH release from HTSE cells and the rate of survival and proliferation of NCI-H292 cells. For in vivo experiment, hypersecretion of airway mucin and goblet cell hyperplasia was induced by exposure of rats to $SO_2$ over 3 weeks. Effects of CGPT and YEJ orally administered for 1 week on in vivo mucin secretion from tracheal goblet cells of rats and hyperplasia of goblet cells were assessed using ELISA and histological analysis after staining the epithelial tissue with alcian blue, respectively. Also, the effects of CGPT and YEJ on contractility of isolated tracheal smooth muscle were investigated. Results : (1) CGPT significantly inhibited in vitro mucin secretion from cultured HTSE cells. However, YEJ did not affect in vitro mucin secretion; (2) CGPT and YEJ did not affect hypersecretion of in vivo mucin and hyperplasia of tracheal goblet cells; (3) CGPT and YEJ slightly increased the expression levels of TNF-alpha or EGF-induced MUC5AC gene in NCI-H292 cells; (4) CGPT and YEJ inhibited acetylcholine-induced contraction of isolated tracheal smooth muscle of rabbit; (5) CGPT and YEJ did not affect LDH release from HTSE cells and the survival and proliferation of NCI-H292 cells. Conclusion : The results from the present study suggest that CGPT and YEJ mainly affect the expression of mucin gene rather than secretion of mucin and do not show remarkable cytotoxicity to respiratory epithelial cells.

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The Effects of Haepyoyijintang on The lnjured Tracheal Tissue lnduced $SO_{2}$ in Rats (해표이진탕이 $SO_{2}$에 의한 흰쥐의 호흡기조직 손상에 미치는 영향)

  • 박동일
    • Journal of Life Science
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    • v.7 no.2
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    • pp.88-94
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    • 1997
  • This experiment was performed in order to study the effects of haepyoyijintang on the injured tracheal tissue induced $SO_{2}$ in rats. Healthy adult male rats weighting about 250g were divided into 4 groups-the Normal group, the Control group, the group of Haepyoyijintang administration for 5days after $SO_{2}$gas exposure (Sample I), and the group of Haepyoyijintang adiministration for 10 days before and for 5days after $SO_{2}$ gas exposure (Sample II). The results were obtained as follows; 1. In the trachea Control group, the lesion of the ciliated epithelium was and the mucus secretion of the respiratory tract was increased iginificantly. 2. In the trachea of SampleIgroup, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control group. 3. In the trachea of SampleIIgroup, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control and Sample Igroup. According to the above results, Haepyoyijintang has significant effects on the injuried tracheal tissu caused by $SO_{2}$ in rats.

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Effects of Bojung-ikgitang-gamibang and Seonbang-paedoktang on Secretion of Airway Mucus and Expression of Mucin Gene (보중익기탕 가미방(補中益氣湯 加味方)과 선방패독탕(仙方敗毒湯)이 기도 점액의 분비와 뮤신 유전자발현에 미치는 영향)

  • Jung, Chang-Ho;Han, Jae-Kyung;Kim, Yun-Hee
    • The Journal of Pediatrics of Korean Medicine
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    • v.21 no.3
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    • pp.33-55
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    • 2007
  • Objectives In the present study, the author intended to investigate whether bojung-ikgitang-gamibang(BJGB) and seonbang-paedoktang(SBPT) significantly affect in vivo and in vitro mucin secretion from airway epithelial cells. Methods In vivo experiment, mice's mucin which is on a hypersecretion of airway mucin, mice's tracheal goblet cells in hyperplasia and mice's intraepithelial mucosubstances were exposed with SO2for3weeks. Effects of orally-administered BJGB and SBPT during 1 week on vivo mucin secretion and hyperplasia of tracheal goblet cells were assessed by using both enzyme-linked immunosorbent assay(ELISA) and staining goblet cells with alcian blue. In vitro experiment, confluent hamster tracheal surface epithelial(HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24hrs and chased for 30 min in the presence of each agent to figure out the effectiveness of 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed. The effects of each agent on contractility of isolated tracheal smooth muscle and effects of each agent on MUC5AC gene expression in cultured HTSE cells were investigated. Also, possible cytotoxicities of each agent were assessed by measuring lactate dehydrogenase(LDH) release. Additionally, effects of BJGB and SBPT on both MUC5AC gene expression in cultured HTSE cells and TNF- or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Results (1) BJGB and SBPT inhibited hypersecretion of in vivo mucin. SBPT also inhibited the increase the number of goblet cells. However, BJGB did not affect the increase of number of goblet cells; (2) BJGB significantly increased mucin secretion from cultured HTSE cells, without significant cytotoxicity, and chiefly affected the 'mucin' secretion; (3) SBPT did not affect mucin secretion from cultured HTSE cells without significant cytotoxicity, and also did not affect the secretion of the other releseable glycoproteins; (4) BJGB and SBPT did not affect Ach-induced contraction of isolated tracheal smooth muscle; (5) SBPT significantly inhibit the expression levels of MUC5AC gene and BJGB significantly increased the expression levels of MUC5AC gene in both HTSE cells and NCI-H292 cells. Conclusions BJGB and SBPT can not only affect the secretion of mucin but also affect the expression of mucin gene. The author suggests that the effects BJGB and SBPT with their components should be further investigated and it is highly desirable to find from oriental medical prescriptions, novel agents which might regulate hypersecretion of mucin from airway epithelial cells.

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The Effects of Scrophulariae Radix on the Injured Tracheal Tissue Induced $SO_2$ in Rats (현삼(玄蔘)이 $SO_2$로 손상된 흰쥐의 호흡기 조직에 미치는 영향)

  • Lee, Min-Woo;Jeon, Jong-Choul;Park, Dong-Il
    • The Journal of Internal Korean Medicine
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    • v.21 no.4
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    • pp.591-595
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    • 2000
  • Objective : This experiment was performed in order to study the effects of Scrophulariae Radix on the injured tracheal tissue induced So, in rats. Methods : Healthy adult male rats weighting about 250g were divided into 4 groups - the Normal group, the Control group, the group of Scrophulariae Radix administration for 5 days after $SO_2$, gas exposure(Sample I), and the group of Scrophulariae Radix administration for 10 days before and for 5 days after $SO_2$, gas exposure(Sample II). Results : In the trachea Control group, the lesion of the ciliated epithelium was severe and the mucus secretion of the respiratory tract was increased significantly. In the trachea of Sample I group, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control group. In the trachea of Sample II group, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control and Sample I group. conclusions : According to the above results, Scrophulariae Radix has significant effects on the injuried tracheal tissue caused by $SO_2$ in rats.

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The Effects of Gyumsuyukgunjun on the injured Tracheal tissue induced $SO_2$ in Rats (김수육군전(金水六君煎)이 $SO_2$에 의한 흰쥐의 호흡기조직손상(呼吸器組織損傷)에 미치는 영향(影響))

  • Lee, Sung-Yong;Park, Dong-Il
    • Korean Journal of Oriental Medicine
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    • v.2 no.1
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    • pp.226-235
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    • 1996
  • This experiment was performed in order to study the effects of Gyumsuyukgunjun on the injured tracheal tissue induced $SO_2$ in rats. Healthy adult male rats weighting about 250g were divided into 4 groups - the Normal group, the Control group, the group of Gyumsuyukgunjun administration for 5 days after $SO_2$ gas exposure (Sample I), and the group of Gyurnsuyukgunjun administration for 10 days before and for 5 days after $SO_2$ gas exposure (Sample II). The results were obtained as follows ; 1. In the trachea Control group, the lesion of the ciliated epithelium was severe and the mucus secretion of the respiratory tract was increased significantly. 2. In the trachea of Sample I group, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control group. 3. In the trachea of Sample II group, the lesion of the ciliated epithelium and the mucus secretion of the respiratory tract were decreased compared with Control and Sample I group. According to the above results, Gyumsuyukgnjun has significant effects on the injuried tracheal tissue caused by $SO_2$ in rats.

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