• Title/Summary/Keyword: Toxicity of heavy water

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Experimental Study on the Effect of Cadmium Containing Shellfish by the LongTerm Intake (카드뮴 (Cadmium)오염 조개 섭취가 생체장기에 미치는 연향의 실험적 연구)

  • Soh, Chin-Thack;Yoo, Il-Soo;Park, Hyun;Kim, Sook-Hyang;Kim, Jae-Jin;Min, Duk-Young
    • The Korean Journal of Malacology
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    • v.9 no.2
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    • pp.85-93
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    • 1993
  • Contamination of heavy metals in water and sediments along Mantyeong (river) has reached up to critical level, The object of the study is to dlucidate some macroinvertebrates which inhabit along the river in respect to the modulatory role in reducing the pollution. For the purpose, Scapharca subcrenata(sea shell) which is common in numbers and biomass in the area was collected, and cadmium (Cd) was subjected as a reference metal in the experiment. The seashell was dried and autoclaved, followed by pellet preparation under various concentration of Cd: 50,100,250,500 and 1,500 ug /pellet. Mice were fed for 3 months, althrough last group(5 mice), 1,500 ug/pellet, died within 4 weeksof the administration.MT-Cd in liver and kidney increased in correlation with the amont of Cd concentration in pellet, especially in kidney than in liver. Liver and kidney of the mice showed pathological changes such as cellular infiltration and focal necrosis in liver, and peri-tubular cell infiltration or tubular necrosis etc. The changes were more intensive by amount of the Cd. Overall results in the present study suggest that Cd may eventually combine with soluble-protein forming metalloprotein, then reduce the toxicity of heavy metal, How the formed Mt-Cd is discharged from the kidney and the mechanism shall be the further subject to be clarified.

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Protective Effect of Panax ginseng extract on Renal Functions Altered by Mercuric Chloride in Albino Rats

  • Saxena, Prabhu-N.;Mahour, K.;Kumar, Ashok
    • Journal of Ginseng Research
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    • v.30 no.3
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    • pp.100-105
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    • 2006
  • Liver and kidney are specific organs which play an active role in biotransformation and detoxification mechanisms. Ant adverse effect of chemicals or heavy metal can cause the delay or fade in these mechanisms. Present study was designed to find out the protective effect of Panax ginseng extract on renal functions altered by mercuric chloride (heavy metal) in albino rat. Fifty albino rats were divided into 10 groups. Five groups for acute study and five groups for sud-acute study viz. control group (Tween 20 and distilled water), mercuric chloride treated group (0.926 mg/kg body wt. for acute and 0.044 mg/kg body wt. for sub-acute group after calculated $LD_{50}$ (9.26 mg/kg body wt.) by probit analysis (Finney, 1971), Panax ginseng extract treated group (10 mg/kg body wt. for acute and sub-acute sets), mercuric chloride treated followed by Panax ginseng extract and Panax ginseng extract followed by mercuric chloride group. All doses were given orally by gavage tube. The result revealed that the serum urea and creatinine significantly increased in mercuric chloride treated group, while significantly decreased (p<0.01) in Panax ginseng extract group after acute and sub-acute treatment. The biochemical estimation is also confirmed by nephropathological aspect. However, the Panax ginseng extract treated followed by mercuric chloride group is more prominent than the mercuric chloride treated followed by Panax ginseng extract group. It can be concluded that Panax ginseng extract had a protective nature on renal functions against mercuric chloride toxicity in albino rats.

Determination of Optimal Toxic Concentration and Accumulation of Cadmium in Broiler Chicks

  • Subhan, Fazli;Khan, Ayaz;Wahid, Fazli;Shehzad, Adeeb;Jan, Amin Ullah
    • Toxicological Research
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    • v.27 no.3
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    • pp.143-147
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    • 2011
  • Cadmium is considered one of the most toxic, non biodegradable heavy metal for the human and animals. The purpose of the present study was to investigate the changes in biochemical parameters of blood and accumulation of cadmium in various tissue caused by various levels of dietary cadmium chloride ($CdCl_2$) in broiler chicks. $CdCl_2$ was administered through drinking water to broiler chicks. In spectral analysis, $CdCl_2$ treatment caused a significant increase in Glutamate pyruvate transaminase (GPT), creatinine and uric acid levels in all treated groups. Intriguingly, the GPT, creatinine, and uric acid levels were significantly higher at 75 mg/kg as compared to the groups treated with high doses (100, 125 and 150 mg/kg) of $CdCl_2$. Atomic Absorption Spectrophotometer (AAS) was used for the determination of Cd accumulation in kidney, liver and Breast muscles. AAS analysis revealed that Cd accumulation is increased in breast muscles as compared to liver and kidney at higher doses of Cd than 75 mg/kg.

Light dependent arsenic uptake and growth in Lactuca sativa L.

  • Hyun-Gi Min;Eunjee Kim;Min-Suk Kim;Jeong-Gyu Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.4
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    • pp.697-705
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    • 2023
  • Along with other heavy metals, arsenic (As) is one among the substances most harmful to living organisms including humans. Owing to its morphological similarity to phosphorus, the uptake of As is influenced by photosynthesis and the phosphorus uptake pathway. In this study, we varied arsenic exposure and light intensity during nutrient solution cultivation of lettuce (Lactuca sativa L.) to determine the effect of these two factors on arsenic uptake, lettuce growth, and electron transfer in photosystem II. In the treatment exposed to 30 μmol L-1 of arsenic, the shoot arsenic concentration increased from 4.73 mg kg-1 to 18.97 mg kg-1 as the light intensity increased from 22 to 122 μmol m-2 s-1. The water content and ET2o/RC of the shoots were not affected by arsenic at low light intensity; however, at optimal light intensity, they decreased progressively with arsenic exposure. Increased light intensity stimulated the growth of plant roots and shoots; contrarily, the difference in growth decreased as the concentration of As exposure increased. The results of this study suggest that the effect of As on plant growth is dependent on light intensity; in particular, an increase in light intensity can increase the uptake of As, thereby affecting plant growth and As toxicity.

Production of Lightweight Aggregates Using Power Plant Reclaimed Ash (발전소 매립회를 이용한 인공경량골재의 제조)

  • Kim, Kang-Duk;Kim, Jung-Hwan;Kim, Yoo-Taek;Kang, Seung-Gu;Lee, Ki-Gang
    • Journal of the Korean Ceramic Society
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    • v.47 no.6
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    • pp.583-589
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    • 2010
  • In this paper, the properties of reclaimed ash(RA) from various domestic thermal power plants(S, D and H) were analyzed, and the possibility of fabricating the artificial lightweight aggregate(ALA) using RA was studied. The chemical compositions of RA are similar to the clay, but it had higher concentrations of alkali earth metal oxides(CaO, MgO) and unburned-carbon. The TCLP(Toxicity Characteristic Leaching Procedure) results showed that the dissolution concentrations of heavy metal ions of RA were below the limitation defined by the enforcement regulation of wastes management law in Korea. The results of IC analysis showed that leaching concentration of $Cl^-$ ion was 124 ppm for RA of HN and ${SO_4}^{2-}$ ion was leached a few hundreds ppm for all RA in this study. The ALAs with various mass ratio of clay to reclaimed ash(RA:Clay = 7:3, 6:4, 5:5, 4:6, 3:7) were sintered with a electric muffle furnace at the temperature of $1050{\sim}1200^{\circ}C$. The specific gravity and water absorption(%) of the sintered ALAs were 1.1~1.8 and 10~30% respectively. The ALA sintered in the rotary kiln at $1125^{\circ}C$ showed a bulk density of 1.7 and water absorption of 15.2%.

S-Nitrosoglutathione (GSNO) Alleviates Lead Toxicity in Soybean by Modulating ROS, Antioxidants and Metal Related Transcripts

  • Methela Nusrat Jahan;Islam Mohammad Shafiqul;Da-Sol Lee;Youn-Ji Woo;Bong-Gyu Mun;Byung-Wook Yun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2023.04a
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    • pp.105-105
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    • 2023
  • Heavy metals, including lead (Pb) toxicity, are increasing in soil and are considered toxic in small amounts. Pb contamination is mainly caused by industrialization - smelting, mining. Agricultural practices - sewage sludge, pests and urban practices - lead paint. It can seriously damage and threaten crop growth. Pb can adversely affect plant growth and development by affecting the photosystem, cell membrane integrity, and excessive production of reactive oxygen species (ROS) such as hydrogen peroxide (H2O2)andsuperoxide(O2.-). NO is produced via enzymatic and non-enzymatic antioxidants to scavenge ROS and lipid peroxidation substrates in terms of protecting cells from oxidative damage. Thus, NO improves ion homeostasis and confers resistance to metal stress. Our results here suggest that exogenous NO may aid in better growth under lead stress. These enhancements may be aided by NO's ability in sensing, signaling and stress tolerance in plants under heavy metal stress in combination with lead stress. Our results show that GSNO has a positive effect on soybean seedling growth in response to axillary pressure and that NO supplementation helps to reduce chlorophyll maturation and relative water content in leaves and roots following strong burst under lead stress. GSNO supplementation (200 µM and 100 µM) reduced compaction and approximated oxidative damage of MDA, proline and H2O2. Under plant tension, a distorted appearance was found in the relief of oxidative damage by ROS scavenging by GSNO application. In summary, modulation of these NO, PCS and prolongation of metal past reversing GSNO application confirms the detoxification of ROS induced by toxic metal rates in soybean. In summary, these NO, PCS and metal traditionally sustained rates of reverse GSNO application confirm the detoxification of ROS induced by toxic metal rates in soybean.

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Proteome analysis of sorghum leaf and root in response to heavy metal stress

  • Roy, Swapan Kumar;Cho, Seong-Woo;Kwon, Soo Jeong;Kamal, Abu Hena Mostafa;Lee, Dong-Gi;Sarker, Kabita;Lee, Moon-Soon;Xin, Zhanguo;Woo, Sun-Hee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.24-24
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    • 2017
  • Heavy metals at toxic levels have the capability to interact with several vital cellular biomolecules such as nuclear proteins and DNA, leading to oxidative stress in plants. The present study was performed to explore the metal tolerance mechanism in Sorghum seedling. Morpho-physiological and metal ions uptake changes were observed prominently in the seedlings when the plants were subjected to different concentrations of $CuSO_4$ and $CdCl_2$. The observed morphological changes revealed that the plants treated with Cu and Cd displayed dramatically altered shoot lengths, fresh weights, and relative water content. In addition, the concentration of Cu and Cd was markedly increased by treatment with Cu and Cd, and the amount of interacting ions taken up by the shoots and roots was significantly and directly correlated with the applied level of Cu and Cd. Using the 2-DE method, a total of 24 and 21 differentially expressed protein spots from sorghum leaves and roots respectively, 33 protein spots from sorghum leaves under Cd stress were analyzed using MALDI-TOF/TOF MS. However, the over-expression of GAPDH plays a significant role in assisting Sorghum bicolor to attenuate the adverse effects of oxidative stress caused by Cu, and the proteins involved in resistance to stress helped the sorghum plants to tolerate high levels of Cu. Significant changes were absorbed in the levels of proteins known to be involved in carbohydrate metabolism, transcriptional regulation, translation and stress responses. In addition, the up-regulation of glutathione S-transferase and cytochrome P450 may play a significant role in Cd-related toxicity and stress responses. The results obtained from the present study may provide insights into the tolerance mechanism of seedling leaves and roots in Sorghum under heavy metal stress.

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Rice Growth Response and Soil Quality by Domestic Wastewater Irrigation on Rice Paddy Field - Lysimeter experiment - (하수종말처리장 방류수를 논 관개용수로 처리시 벼 생육 및 토양환경 영향 조사 - 라이시미터 재배실험 -)

  • Cho, Jae-Young;Park, Seung-Woo;Son, Jae-Gwon;Park, Bong-Ju;Li, Long-Gen
    • Journal of Korean Society of Rural Planning
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    • v.12 no.2 s.31
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    • pp.49-56
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    • 2006
  • The application of domestic wastewater on rice paddies results in the accumulation of sodium(Na$^+$) to the soil. Excessive concentration of sodium may cause the deterioration of the physical characteristics of the soil, change in the osmosis of the soil, destruction of soil aggregates as well as ion toxicity due to sodium accumulation. Using domestic wastewater as irrigation water should be preceded by measures to prevent or control the soil salinization caused by sodium. Agricultural reuse of domestic wastewater were found not to cause serious problems with food safety due to heavy metals. However, pre-treatment using ultraviolet or ozone is recommended to reduce the number of bacteria and gem and for public health reasons. Using domestic wastewater has shown that reducing the standard application of chemical fertilizers by as much as 50% reduced the harvesting index by only 10%. This study has shown that it is feasible to reuse domestic wastewater on rice paddies. In order to facilitate the application, it is deemed necessary to establish wastewater treatment technologies in the future, to review criteria for recycling domestic wastewater for agricultural purposes such as conditions of soil and cropping system and to resolve conflicts with farmers and public health issues.

Analyses on Thermal Stability and Structural Integrity of the Improved Disposal Systems for Spent Nuclear Fuels in Korea

  • Lee, Jongyoul;Kim, Hyeona;Kim, Inyoung;Choi, Heuijoo;Cho, Dongkeun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.18 no.spc
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    • pp.21-36
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    • 2020
  • With respect to spent nuclear fuels, disposal containers and bentonite buffer blocks in deep geological disposal systems are the primary engineered barrier elements that are required to isolate radioactive toxicity for a long period of time and delay the leakage of radio nuclides such that they do not affect human and natural environments. Therefore, the thermal stability of the bentonite buffer and structural integrity of the disposal container are essential factors for maintaining the safety of a deep geological disposal system. The most important requirement in the design of such a system involves ensuring that the temperature of the buffer does not exceed 100℃ because of the decay heat emitted from high-level wastes loaded in the disposal container. In addition, the disposal containers should maintain structural integrity under loads, such as hydraulic pressure, at an underground depth of 500 m and swelling pressure of the bentonite buffer. In this study, we analyzed the thermal stability and structural integrity in a deep geological disposal environment of the improved deep geological disposal systems for domestic light-water and heavy-water reactor types of spent nuclear fuels, which were considered to be subject to direct disposal. The results of the thermal stability and structural integrity assessments indicated that the improved disposal systems for each type of spent nuclear fuel satisfied the temperature limit requirement (< 100℃) of the disposal system, and the disposal containers were observed to maintain their integrity with a safety ratio of 2.0 or higher in the environment of deep disposal.

Efficacy of Three Different Plant Species for Arsenic Phytoextraction from Hydroponic System

  • Tiwari, Sarita;Sarangi, Bijaya Ketan;Pandey, Ram Avatar
    • Environmental Engineering Research
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    • v.19 no.2
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    • pp.145-149
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    • 2014
  • Arsenic (As) is one of the heavy metals which causes acute bio-toxicity even at low concentration and has disastrous effect on environment. In some countries, As contamination has become alarming and increasing day by day as consequences of unsustainable management practices. Many existing physical, chemical and biological processes for As removal from water system are not feasible due to techno-economic limitations. The present study highlights the scope of biological strategy for As removal through phytoextraction. Arsenic uptake and accumulation in the biomass of three plant species and their As tolerance abilities have been investigated to develop an efficient phytoextraction system in combination of these plant species. Three non-crop plant species, Pteris vittata; Mimosa pudica, and Eichhornia crassipus were treated with 0-200 mg/L As in liquid nutrient solution for 14 days. P. vittata accumulated total 9,082.2 mg (8,223 mg in fronds) As/kg biomass and Eichhornia total 6,969 mg (4,517 mg in fronds)/kg biomass at 200 mg/L As concentration, respectively. Bioaccumulation factor (BF) and translocation factor (TF) were estimated to differentiate between excluders, accumulators and accumulation in above ground biomass. Pteris and Eichhornia have highest BF (67 and 17) and TF (64 and 3), respectively. In contrast, Mimosa accumulated up to 174 mg As/kg plant biomass which is low in comparison with other two plants, and both BF and TF were ${\leq}1$. This study reveals that Pteris and Eichhornia are As hyperaccumulator, and potential candidates for As removal from water system.