• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.314-321
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• 2008

Acaricidal effects of materials derived from Diospyros kaki roots against Dermatophagoides farinae and D. pteronyssinus were assessed using impregnated fabric disk bioassay and compared with that of the commercial benzyl benzoate. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the chloroform extract of Diospyros kaki roots were 1.66 and $0.96{\mu}g/cm^2$ against D. farinae and D. pteronyssinus. The chloroform extract of Diospyros kaki roots was approximately 15.2 more toxic than benzyl benzoate against D. farinae, and 7.6 times more toxic against D. pteronyssinus. Purification of the biologically active constituent from D. kaki roots was done by using silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by GC-MS, $^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C$ COSY-NMR, and DEPT-NMR spectra, and identified as plumbagin. The acaricidal activity of plumbagin and its derivatives (naphthazarin, dichlon, 2,3-dibromo-1,4-naphthoquinone, and 2-bromo-1,4-naphthoquinone) was examined. On the basis of $LD_{50}$ values, the most toxic compound against D. farinae was naphthazarin $(0.011{\mu}g/cm^2)$ followed by plumbagin $(0.019{\mu}g/cm^2),$ 2-bromo-1,4-naphthoquinone $(0.079{\mu}g/cm^2)$, dichlon $(0.422{\mu}g/cm^2)$, and benzyl benzoate $(9.14{\mu}g/cm^2)$. Additionally, the skin color of the dust mites was changed from colorless-transparent to dark brown-black by the treatment of plumbagin. Similar results have been exhibited in its derivatives (naphthazarin, dichlon, and 2-bromo-1,4-naphthoquinone). In contrast, little or no discoloration was observed for benzyl benzoate. From this point of view, plumbagin and its derivatives can be very useful for the potential control agents, lead compounds, and indicator of house dust mites.

• Environmental Analysis Health and Toxicology
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• 제30권sup호
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• pp.3.1-3.11
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• 2015

Objectives This study presents the indicators and proxy variables for the quantitative assessment of green chemistry technologies and evaluates the relative importance of each assessment element by consulting experts from the fields of ecology, chemistry, safety, and public health. Methods The results collected were subjected to an analytic hierarchy process to obtain the weights of the indicators and the proxy variables. Results These weights may prove useful in avoiding having to resort to qualitative means in absence of weights between indicators when integrating the results of quantitative assessment by indicator. Conclusions This study points to the limitations of current quantitative assessment techniques for green chemistry technologies and seeks to present the future direction for quantitative assessment of green chemistry technologies.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1394-1398
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• 2007

Acaricidal activity of the active constituent derived from Pyrus ussuriensis fruits against Dermatophagoides farinae and D. pteronyssinus was examined and compared with that of the commercial benzyl benzoate. The $LD_{50}$ value of the ethyl acetate fraction obtained from the aqueous extract of P. ussuriensis fruits was 9.51 and $8.59{\mu}g/cm^3$ against D. farinae and D. pteronyssinus, respectively. The active constituent was identified as quinone by spectroscopic analyses. On the basis of $LD_{50}$ values with quinone and its congeners, the compound most toxic against D. farinae was quinone ($1.19{\mu}g/cm^3$), followed by quinaldine (1.46), benzyl benzoate (9.32), 4-quinolinol (86.55), quinine (89.16), and 2-quinolinol (91.13). Against D. pteronyssinus, these were quinone ($1.02{\mu}g/cm^3$), followed by quinaldine (1.29), benzyl benzoate (8.54), 4-quinolinol (78.63), quinine (82.33), and 2-quinolinol (86.24). These results indicate that the acaricidal activity of the aqueous extracts can be mostly attributed to quinone. Quinone was about 7.8 and 8.4 times more toxic than benzyl benzoate against D. farinae and D. pteronyssinus. Additionally, quinaldine was about 6.4 and 6.6 times more toxic than benzyl benzoate against D. farinae and D. pteronyssinus, respectively. Furthermore, the skin color of the dust mites was changed from colorless-transparent to dark brown-black by the treatment of quinone. These results indicate that quinone can be very useful as potential control agents, lead compounds, or the indicator of house dust mites.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1591-1596
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• 2006

Acaricidal effects of materials derived from Caesalpinia sappan heartwoods against Dermatophagoides farinae and D. pteronyssinus were assessed and compared with those evidenced by commercial benzyl benzoate and DEET. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the methanol extracts derived from C. sappan heartwoods were 6.13 and $5.44{\mu}g/cm^3$ against D. farinae and D. pteronyssinus, respectively. Furthermore, the ethyl acetate fraction derived from the methanol extract was approximately 8.71 more toxic than DEET against D. farinae, and 4.73 times more toxic against D. pteronyssinus. The biologically active constituent from the ethyl acetate fraction of C. sappan heartwood extract was purified via silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by $GC-MS,\;^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C\;COSY-NMR$, and DEPT-NMR spectroscopy, and identified as juglone (5-hydroxy-l,4-naphthoquinone). Based on the $LD_{50}$ values of juglone and its derivatives, the most toxic compound against D. farinae was juglone ($0.076{\mu}g/cm^3$), followed by benzyl benzoate ($9.143{\mu}g/cm^3$) and 2methyl-l,4-naphthoquinone ($40.0{\mu}g/cm^3$). These results indicate that the acaricidal activity of C. sappan heartwoods is likely to be the result of the effects of juglone. Additionally, juglone treatment was shown to effect a change in the color of the cuticles of house dust mites, from colorless-transparent to dark brownish-black. Accordingly, as a naturally occurring acaricidal agent, C. sappan heartwood-derived juglone should prove to be quite 'useful as a potential control agent, lead compound, and house dust mite indicator.

• Fisheries and Aquatic Sciences
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• 제13권3호
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• pp.236-243
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• 2010

Fecal sterols, nonylphenolic compounds (NPs), and polycyclic aromatic hydrocarbons (PAHs) were determined in surface water from Masan Bay and its adjacent rivers in February 2005. Concentrations of coporstanol (Cop), an indicator of fecal pollution, in surface water ranged from <10 to 13,853 ng/L, and concentrations of nonylphenol, the most toxic of the NPs, ranged from 10.2 to 481 ng/L, and concentrations of PAHs ranged from 8.61 to 223 ng/L. The concentrations of the compounds measured in this study were lower than or comparable to those at other locations in Korea and other countries. The contamination of Cop and PAHs in surface water was associated with the discharge from rivers passing through cities and/or industrial complexes. The NP contamination was associated with wastewater treatment plant (WWTP) effluents through outfalls as well as riverine discharge. Compared to ecotoxicological values, the concentrations of NPs from rivers, the mouths of rivers, and WWTP outfall areas exceeded guidelines, suggesting that hot spot areas may pose a potential risk to sensitive species.

• 환경생물
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• 제42권1호
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• pp.54-61
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• 2024

생태계는 생물에게 매우 다양한 환경조건의 서식처를 제공하며 이 서식처 내에 적응한 생물만이 생존, 성장, 번식하며 해당 서식처에 살아가게 된다. 또한 서식처의 환경조건들은 서식처로 유입한 화학물질의 생물유효도(bioavailability)에 크게 영향을 미쳐 생물들이 받는 부정적 영향이 서로 다를 수 있다. 본 연구에서는 생태독성평가에 적합한 ISO guideline 11276의 독성평가 표준종 중 토양 생태계에 다양한 서식처 조건 중 수변 생태계에서 적응하여 살아가는 Yuukianura szeptyckii의 중금속에 대한 평가 민감성을 확인하고 수변 서식처 중금속 생태독성평가종으로서의 가능성을 확인하는 데 있다. 결과는 카드뮴과 구리 모두 농도에 따른 유의한 독성영향을 보였다. 카드뮴의 경우 LC₅₀은 280 mg kg^-1, EC₅₀은 66 mg kg^-1, NOEC와 LOEC는 각각 25, 50 mg kg^-1으로 나타났으며 구리의 경우에는 LC₅₀은 911 mg kg^-1, EC₅₀은 151 mg kg^-1, LOEC는 50 mg kg^-1으로 나타났다. 기존의 국제표준종인 Folsomia candida와 국내표준종인 Allonychiurus kimi의 이전 결과와 비교하면 더욱 민감한 독성값을 보였으며, 이러한 생존과 번식에 미치는 부정적 영향은 체내의 중금속의 유입 농도와 밀접한 관련을 보였다. Y. szeptyckii는 수분 함량이 높은 토양에서 중금속 생태독성평가에 민감한 종으로 나타났으며, 상대적으로 높은 습도를 보일 가능성이 높은 수변생태계에 적합한 생태독성평가종으로 사용이 가능할 것으로 생각된다.

• 대한수의학회지
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• 제36권4호
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• pp.873-886
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• 1996

For better understanding the interrelationship of hemorrhage and aggregation mechanism, cyclopiazonic acid(CPA) known as promoting the aggregation of platelet, aflatoxin $B_1(AFB_1)$ inhibiting platelet aggregation were used as toxic mycotoxins in these studies. In order to investigate the potential role of prostaglandin metabolism on the platelet aggregation, a variety of prostaglandin metabolites such as $PGF_{2{\alpha}}$, $PGE_2$ and $TXB_2$ were measured in homogenized rabbit platelets by TLC and LSC. And the role of $Ca^{{+}{+}}$ on the platelet aggregation was investigated by flow cytometer. Finally, the morphological effects of mycotoxins on platelet were determined by transmission electron microscope. The results and conclusions obtained from these studies are: 1) CPA induced no changes but $AFB_1$ increased $PGE_2$ and $TXB_2$. 2) CPA promoted ADP, collagen, thrombin, A.A., and PAF-induced $Ca^{{+}{+}}$ release. $AFB_1$, however, decreased $Ca^{{+}{+}}$ level except collagen-induced $Ca^{{+}{+}}$ release. When the calcium blocker, verapamil, was used, CPA decreased thrombin-induced $Ca^{{+}{+}}$ release and increased collagen, ADP, PAF and A.A.-induced $Ca^{{+}{+}}$ release. $AFB_1$ in contrast decreased the all factors induced $Ca^{{+}{+}}$ release. 3) $AFB_1$ did not induce any ultrastructural changes except large vacuole formation in a few platelets. And CPA also did not induce any changes except moderate shape change, indicator of platelet activation. In conclusion, CPA promoted platelet aggregation by the increases of $Ca^{{+}{+}}$ release but had no changes in A.A. metabolites. Antiaggregating effects of $AFB_1$ may be due to decreases of $Ca^{{+}{+}}$ release and increases of $PGE_2$ and $PGF_{2{\alpha}}$ formation. These data provide the basis for the future study of mobilization and function of $Ca^{{+}{+}}$ in platelet aggregation.