• Journal of Korean Society of Environmental Engineers
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• v.22 no.11
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• pp.2047-2058
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• 2000

The effects of operating parameters on performance of upflow anaerobic sludge blanket(UASB). anaerobic filter(AF), and two-stage anaerobic sludge bed filter (ASBF) bioreactors in treating swine wastewater were evaluated by operating the lab-scale bioreactors upto hydraulic retention time(HRT) of 1 day and organic loading rate (OLR) of $5.1kg-COD/m^3{\cdot}d$ for 200 days. Swine wastewaters of which characteristics were affected by types of hog raising and seasons contained high concentrations of COD, SS, and ammonia. Inoculation of the bioreactors with waste sludge from anaerobic treatment facility of local municipal wastewater treatment plant was effective in developing biomass in the bioreactors. Acclimation period of the bioreactors with swine wastewaters required approximately 40 days, but that for AF and two-stage ASBF, which were filled with media, was faster than VASB. The bioreactors showed high and stable COD removal efficiency of 77~91% at influent T-N concentrations of 370~800mg/L but low and unstable COD removal efficiency of 24~94% at influent T-N concentrations of 760~1,310mg/L. It is essential to remove ammonia prior to anaerobic treatment since the concentrations of ammonia in swine wastewaters showed toxic effects to methanogenic bacteria. The bioreactors were effective in treating swine wastewaters with COD removal efficiency of 78.9~81.5% and biogas generation rate of $0.39{\sim}0.59m^3/kg-COD_r$ at OLR of $1.1{\sim}2.2kg-COD/m^3{\cdot}d$: however, an increase of OLR by reducing HRT and increasing influent COD caused decrease of COD removal efficiency. The extent of decrease in COD removal efficiency was higher in UASB than AF and two-stage ASBF. AF and two-stage ASBF anaerobic bioreactors were effective in treating varing characteristics of swine wastewaters since they showed high and stable COD removal efficiency at high OLR due to effective retention of biomass by media and staging.

• Environmental Analysis Health and Toxicology
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• v.15 no.4
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• pp.131-137
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• 2000

The release of hazardous waste materials into the environment poses serious risks in humans and ecosystems. The risk assessment of environmental pollutants including hazardous chemicals requires a comprehensive measurement of hazard and exposure of the chemicals that can be achieved by toxicity evaluation using a biological system such as biomarkers. In this report we have tried to develop a biomarker used to elucidate a molecular basis of, and to monitor abnormal behaviors caused by diazinon in Japanese medaka (Oryzias latipes) as a model organism. First, an attempt was made to clone tyrosine hydroxylase gene from Japanese medaka that would be a candidate for a biomarker for neuronal modulations and behaviors. For monitoring experiments at behavioral and molecular biological levels, the fish were treated under different sublethal conditions of diazinon and their behavioral responses were observed . In this study we have successfully cloned a partial TH gene from the medaka fish through PCR screening of an ovary cDNA library. DNA sequencing analysis revealed that the amplified fragment was 327 bp encoding 109 amino acids. Comparing the DNA sequence of medaka TH with other species, TH gene revealed the DNA sequence was completely identical to that of rat TH. In the RT-PCR, 330 Up of mRNA was consistently amplified in all the treated samples including control There were no significant differences in the TH expression level regardless of treating concentrations (1∼5,000 ppb) and time (0∼48 hr) The reason appeared to be that RT-PCR was not performed using through a quantitative analysis normalized against an actin gene expression. Organ or tissue - specific detection of TH activity and mRNA as biomarkers will be a useful monitoring tool for neurobehavioral changes in fish influenced by toxic chemicals. Furthermore, quantitative analysis of locomotive patterns and its correlation with the neurochemical and molecular data would be highly useful in measuring toxicity and hazard ofvarious environmental pollutants.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.9
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• pp.1035-1043
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• 2007

A leachate containing an elevated concentration of organic and inorganic compounds has the potential to contaminate adjacent soils and groundwater as well as downgradient areas of the watershed. Moreover high-strength ammonium concentrations in leachate can be toxic to aquatic ecological systems as well as consuming dissolved oxygen, due to ammonium oxidation, and thereby causing eutrophication of the watershed. In response to these concerns landfill stabilization and leachate treatment are required to reduce contaminant loading sand minimize effects on the environment. Compared with other treatment technologies, leachate recirculation technology is most effective for the pre-treatment of leachate and the acceleration of waste stabilization processes in a landfill. However, leachate recirculation that accelerates the decomposition of readily degradable organic matter might also be generating high-strength ammonium in the leachate. Since most landfill leachate having high concentrations of nitrogen also contain insufficient quantities of the organic carbon required for complete denitrification, we combined a shortcut biological nitrogen removal (SBNR) technology in order to solve the problem associated with the inability to denitrify the oxidized ammonium due to the lack of carbon sources. The accumulation of nitrite was successfully achieved at a 0.8 ratio of $NO_2^{-}-N/NO_x-N$ in an on-site reactor of the sequencing batch reactor (SBR) type that had operated for six hours in an aeration phase. The $NO_x$-N ratio in leachate produced following SBR treatment was reduced in the landfill and the denitrification mechanism is implied sulfur-based autotrophic denitrification and/or heterotrophic denitrification. The combined leachate recirculation with SBNR proved an effective technology for landfill stabilization and nitrogen removal in leachate.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2000.11a
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• pp.44-63
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• 2000

Situated close to Heathrow Airport, and adjacent to the M4 and M25 Motorways, the site at Axis Park is considered a prime location for business in the UK. In consequnce two of the UK's major property development companies, MEPC and Redrew Homes sought the expertise of Intergeo to remediate the contaminated former industrial site prior to its development. Industrial use of the twenty-six hectare site, started in 1936, when Hawker Aircraft commence aircraft manufacture. In 1963 the Firestone Tyre and Rubber Company purchased part of the site. Ford commenced vehicle production at the site in the mid-1970's and production was continued by Iveco Ford from 1986 to the plant's decommissioning in 1997. Geologically the site is underlain by sand and gravel, deposited in prehistory by the River Thames, with London Clay at around 6m depth. The level of groundwater fluctuates seasonally at around 2.5m depth, moving slowly southwest towards local streams and watercourses. A phased investigation of the site was undertaken, which culminated in the extensive site investigation undertaken by Intergeo in 1998. In total 50 boreholes, 90 probeholes and 60 trial pits were used to investigate the site and around 4000 solid and 1300 liquid samples were tested in the laboratory for chemical substances. The investigations identified total petroleum hydrocarbons in the soil up to 25, 000mg/kg. Diesel oil, with some lubricating oil were the main components. Volatile organic compounds were identified in the groundwater in excess of 10mg/l. Specific substances included trichloromethane, trichloromethane and tetrachloroethene. Both the oil and volatile compounds were widely spread across the site, The specific substances identified could be traced back to industrial processes used at one or other dates in the sites history Slightly elevated levels of toxic metals and polycyclic aromatic hydrocarbons were also identified locally. Prior to remediation of the site and throughout its progress, extensive liaison with the regulatory authorities and the client's professional representatives was required. In addition to meetings, numerous technical documents detailing methods and health and safety issues were required in order to comply with UK environmental and safety legislation. After initially considering a range of options to undertake remediation, the following three main techniques were selected: ex-situ bioremediation of hydrocarbon contaminated soils, skimming of free floating hydrocarbon product from the water surface at wells and excavations and air stripping of volatile organic compounds from groundwater recovered from wells. The achievements were as follows: 1) 350, 000m3 of soil was excavated and 112, 000m3 of sand and gravel was processed to remove gravel and cobble sized particles; 2) 53, 000m3 of hydrocarbon contaminated soil was bioremediated in windrows ; 3) 7000m3 of groundwater was processed by skimming to remove free floating Product; 4) 196, 000m3 of groundwater was Processed by air stripping to remove volatile organic compounds. Only 1000m3 of soil left the site for disposal in licensed waste facilities Given the costs of disposal in the UK, the selected methods represented a considerable cost saving to the Clients. All other soil was engineered back into the ground to a precise geotechnical specification. The following objective levels were achieved across the site 1) By a Risk Based Corrective Action (RBCA) methodology it was demonstrated that soil with less that 1000mg/kg total petroleum hydrocarbons did not pose a hazard to health or water resources and therefore, could remain insitu; 2) Soils destined for the residential areas of the site were remediated to 250mg/kg total petroleum hydrocarbons; in the industrial areas 500mg/kg was proven acceptable. 3) Hydrocarbons in groundwater were remediated to below the Dutch Intervegtion Level of 0.6mg/1; 4) Volatile organic compounds/BTEX group substances were reduced to below the Dutch Intervention Levels; 5) Polycyclic aromatic hydrocarbons and metals were below Inter-departmental Committee for the Redevelopment of Contaminated Land guideline levels for intended enduse. In order to verify the qualify of the work 1500 chemical test results were submitted for the purpose of validation. Quality assurance checks were undertaken by independent consultants and at an independent laboratory selected by Intergeo. Long term monitoring of water quality was undertaken for a period of one year after remediation work had been completed. Both the regulatory authorities and Clients representatives endorsed the quality of remediation now completed at the site. Subsequent to completion of the remediation work Redrew Homes constructed a prestige housing development. The properties at "Belvedere Place" retailed at premium prices. On the MEPC site the Post Office, amongst others, has located a major sorting office for the London area. Exceptionally high standards of remediation, control and documentation were a requirement for the work undertaken here.aken here.

• Journal of Soil and Groundwater Environment
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• v.7 no.4
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• pp.31-39
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• 2002

Pollutants from industry, mining, agriculture, and other sources have contaminated sediments in many surface water bodies. Sediment contamination poses a severe threat to human health and environment because many toxic contaminants that are barely detectable in the water column can accumulate in sediments at much higher levels. The purpose of this study was to make optimal treatment and disposal plan o( sediment for water quality improvement in small-scale resevoir based on an evaluation of degree of contamination. The degree of contamination were investigated for 23 samples of 9 site at different depth of sediment in small-scale J river. Results for analysis of contaminated sediments were observed that copper concentration of 4 samples were higher than the regulation of hazardous waste (3 mg/L) and that of all samples were exceeded soil pollution warning levels for agricultural areas. Lead and mercury concentration of all samples were detected below both regulations. Necessary of sediment dredge was evaluated for organic matter and nutrient through standard levels of Paldang lake and the lower Han river in Korea and Tokyo bay and Yokohama bay in Japan. The degree of contamination for organic matter and nutrient was not serious. Compared standard levels of Japan, America, and Canada for heavy metal, contaminated sediment was concluded as lowest effect level or limit of tolerance level because standard levels of America and Canada was established worst effect of benthic organisms. The optimal treatment method of sediment contained heavy metal was cement-based solidification/stabilization to prevent heavy metal leaching.