• Title/Summary/Keyword: Total coliform bacteria

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Nutritional and Microbiol Quality Assessment of Chungmukimbab (충무김밥의 영양적 및 미생물적 품질 평가)

  • Choi Jong-Duck;Hwang Young-Suk;Jeoung In-Geon;Lee Da-Jeong;Park Phil-Sook;Park Mi-Yeon
    • Journal of Food Hygiene and Safety
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    • v.21 no.3
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    • pp.189-195
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    • 2006
  • This research was designed to evaluate the nutritional an microbiol quality assessment of Chungmukimbab purchased from market in Tongyeoung area. Contents of calories, calcium, iron, thiamin and riboflavin in ordinary kimbab and Chungmukimbab were lower than the recommended levels of Korean adult men. So, We suggested that a fruit, beverage and ffod stuff were supplemented to maintain nutritional balance. Total aerobic bacteria and coliform group of just prepared ordinary kimbab and Chungmukimbab samples from market were not significantly different, showing approximately $5.50{\pm}0.38 log_{10} CFU/g,\;2.10{\pm}0.47log_{10}MPN/100g$ in ordinary kimbab, $5.61{\pm}0.42log_{10}CFU/g,\;1.75{\pm}0.34 log_{10} MPN/100g$ in Chungmukimbab, respectively. Total aerobic bacteria of law ingredients of chungmukimbab sample were 3 to $4 log_{10}CFU/g$ in kimbab, seasoning squid and radish roots kimchi, 4 to $5 log_{10}CFU/g$ in boiled fish paste. The coliform groups were 1 to $2 log_{10}$ MPN/100 g in kimbab, seasoning squid and radish roots kimchi, 2 to $3 log_{10}$ MPN/100g in boiled fish paste. Detection rate of E. coli and Staphylococcus aureus counts were 10.0, 12.5% in Chungmu-kimbab, 15.0, 10.0% in seasoning squid, 0, 10.0% in radish roots kimchi respectively, not detected in boiled fish paste samples. During storage at $15^{\circ}C$ for 24 hours, total aerobic counts and coliform groups in ordinary kimbab and Chungmukimbab were increased by the 1.94, $0.97log_{10}CFU/g$, 0.60, 0.72 log10 MPN/100g respectively. Total aerobic counts of Chungmukimbab ingredients increased $0.83{\sim}l.33 log_{10}CFU/g$ at different time

Bacterial contamination levels in strawberry parts according to their cultivation methods (재배방식에 따른 딸기의 부위별 세균 오염도 분석)

  • Yu, Yong-Man;Kim, Jin-Won;Choi, In-Wook;Youn, Young-Nam;Lee, Young-Ha
    • Food Science and Preservation
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    • v.20 no.3
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    • pp.323-329
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    • 2013
  • Strawberries are among the leading ready-to-eat agricultural products that have superior taste and nutrition. Thus, consumer concerns about the safety of eating strawberries are growing. To evaluate the contamination levels of strawberries according to their cultivation methods (nutriculture, pesticide-free culture and organic farming) and parts [fruit (flesh), stalk (pedunle) and leaf (calyx)], 1,020 parts of strawberry samples were collected at 12 farms in Nonsan-si and quantitatively or qualitatively examined for the indicators of food safety and food poisoning bacteria. The total aerobic bacteria count in the whole samples was 2.3~6.8 ${\log}_{10}$ CFU/g, and coliform bacteria were detected in 14.2% of the whole samples with a contamination level range of 2.1~4.5 log CFU/g. E. coli were detected in 0.9% of the whole samples with a contamination level range of 2.1~2.8 log CFU/g. The analysis of the bacterial levels according to the cultivation methods showed that the total aerobic bacteria and coliform counts were higher in the strawberries that were grown via organic farming than in those that were grown via nutriculture and pesticide-free culture. However, the E. coli counts of the strawberries that were grown via organic farming and via pesticide-free culture were similar and differed from that of the strawberries that were grown via nutriculture. The analysis of the contamination levels according to the parts of the strawberries showed that the total aerobic bacteria, coliform and E. coli counts of the fruits, stalks and leaves of the strawberries did not significantly differ. Staphylococcus aureus was detected in two organically grown strawberries, but Salmonella spp., Listeria monocytogenes and E. coli O157:H7 were not detected in the whole samples. These results show that the bacterial contamination levels of the strawberries differed based on their cultivation methods. Thus, a suitable method of reducing the bacterial contamination levels of strawberries according to their farming methods is needed.

Studies on the Keeping Quality of Ultra-High-Temperature Sterilized Milk (초고온멸균유(超高溫滅菌乳)의 보존성(保存性)에 관한 연구(硏究))

  • Kim, Jong-U
    • Korean Journal of Agricultural Science
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    • v.9 no.2
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    • pp.556-561
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    • 1982
  • These studies were carried out to investigate the freshness and bacterial counts of ultrahigh- temperature sterilized market milk which were treated and distributed in three districts; Cheonan, Pyungtak and Suwon in Korea, and to elucidate theirs keeping qualities when stored in refregerator and at room temperature. And the results were obtained were as follows; 1. The acidities in milk from three plants after stored at $5^{\circ}C$ were retained 0.16% up to 6 weeks and 0.18% up to 10 weeks. And negative reactions were appeared for alcohol and boiling tests also up to 10 weeks. 2. After milk were stored at $25^{\circ}C$, the acidities were 0.18% up to 9 weeks and also negative reaction were appeared for both alcohol and boiling tests up to 9 weeks. 3. When milk were stored at $5^{\circ}C$, no thermoduric and coliform bacteria were detected from all samples. And the total viable number of organisms and the number of psychrophilic bacteria were $<10/m{\ell}$ for 5, 6, 7 and 10 weeks respectively. 4. Also thermoduric and coliform bacteria were not detected after milk were stored at $25^{\circ}C$ for 10 weeks. And the total viable number of organisms and the number of psychrophilic bacteria were $<10/m{\ell}$ after from 2 weeks to 10 weeks.

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Microbiological Water Quality of Water Purifiers at Elementary Schools in Gunsan Area (군산지역 초등학교 정수기 물의 미생물학적 수질)

  • Seo, Lan-Ju;Park, Suhk-Hwan;Lee, Geon-Hyoung
    • Korean Journal of Microbiology
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    • v.45 no.1
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    • pp.74-81
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    • 2009
  • In this research, we investigated the actual conditions of water purification systems at ten elementary schools located in Gunsan, Korea from July to December, 2007. The results were as follows; The population densities of heterotrophic bacteria in water purifiers ranged from 0 to $1.2{\pm}0.2{\times}10^4$ CFU/ml and those of tap water were in the range from 0 to $1.9{\pm}0.3{\times}10^4$ CFU/ml during investigation periods. Ninety percentage of purified water samples in July and September, 87.2% in October and November, and 93.7% in December turned out not to be suitable for drinking. The seasonal variation of the population densities of heterotrophic bacteria from purified waters was not notable. The total coliform, Salmonella and Shigella were not detected in purified water and tap water during investigation periods. Forty-five species of bacteria were isolated from water purifiers. The identified bacterial genera were Sphingomonas, Methylobacterium, Caulobacter, Novosphingobium, Bosea, Brevundimonas, Aminobacter, Ralstonia, Mitsuaria, Variovorax, Acidovorax, Massilia, Pseudomonas, Acinetobacter, Aeromonas, Bacillus, Staphylococcus, Brevibacillus, Microbacterium, Lapillicoccus, Micrococcus, Arthrobacter, Janibacter, Flavobacterium, Chryseobacterium, and Hymenobacter: Among the isolates, opportunistic pathogens such as Pseudomonas fluorescens, Staphylococcus epidermidis, Flavobacterium johnsoniae, and Acinetobacter johnsonii were also found.

Microbiological and Sensory Characteristics of Electron Beam Irradiated Squid Jeotkal and Its Ingredients (전자선 조사 양념 오징어 젓갈 및 부재료의 미생물학적 및 관능적 특성)

  • Kim, Bin-Na;Jung, Samooel;Choe, Jun-Ho;Liu, Xian De;Jo, Cheo-Run
    • Korean Journal of Agricultural Science
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    • v.35 no.2
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    • pp.155-165
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    • 2008
  • A seasoned squid Jeotkal, Korean traditional fermented seafood, and its ingredients for manufacturing, including red hot pepper powder, ground garlic, sesame seeds, were irradiated with 0, 0.5, 1, 2, and 5 kGy by electron beam and stored at $4^{\circ}C$ for 4 weeks to determine the changes in microbiological and sensory characteristics. The initial contamination of squid Jeotkal such as total aerobic bacteria, yeast & mold, and coliform bacteria were at the levels of 2.88, 3.04, and 4.20 log CFU/g, respectively. However, electron beam irradiation with does at 5 kGy reduced the total aerobic bacteria to about 1 log CFU/g. Yeast & mold and coliform bacteria were reduced to 1-2 log CFU/g after 2 kGy of irradiation and reached to undetected level when the sample was irradiated at 5 kGy and following storage at $4^{\circ}C$ for 4 weeks. Sensory characteristics showed that electron beam irradiation of up to 5 kGy did not adversely affect overall acceptability of squid Jeotkal and its ingredients during cold storage. Therefore, electron beam irradiation is one of the possible means to improve storage stability of seasoned squid jeotkal, which has limited alternative sterilization methods due to the temperature characteristics of the products.

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Potential Pathogen Monitoring of Powdered Infant Formula Milk and Related Products in Korea (국내산 조제유류에서의 위해 미생물 모니터링)

  • Kim, Young-Jo;Moon, Jin-San;Park, Hyun-Jung;Heo, Eun-Jeong;Kim, Ji-Ho;Lee, Hee-Soo;Wee, Sung-Hwan
    • Journal of Food Hygiene and Safety
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    • v.25 no.4
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    • pp.341-345
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    • 2010
  • Three-hundred samples of powdered infant formula milk and related products from four different manufacturers in 2010 were collected and surveyed their contaminations for aerobic bacteria, coliform, Enterobacter(Cronobacter) sakazakii, and food-borne pathogens. Fifteen samples of sterilized infant formula milk were all negative on these microorganisms. In all collected products of un sterilized infant formulas and follow-on infant formulas, aerobic bacteria were detected at 239 (83.9%) among 285 samples, and they all were found below $10^3$ cfu/g. Coliform bacteria were also detected at four among 285 samples. Salmonella spp. and Ent. sakazakii, weren't detected at the all samples. Bacillus cereus was detected at 24 (8.4%) among 285 samples. The level of B. cereus was below 100 cfu/g but it was suitable for the range of specification of B. cereus in infant formulas. Clostridium perjringens, Escherichia coli O157:H7, Staphylococcus aureus and Listeria monocytogenes weren't also detected. In consequence, it was suitable for total viable count, coliform and potential pathogen to the specification of infant formulas and related products.

하계 경기만의 수질오염과 생산력에 관한 연구 1

  • 이민재;홍순우;하영칠
    • Korean Journal of Microbiology
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    • v.10 no.3
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    • pp.97-104
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    • 1972
  • The extent of water pollution was investigated at 4 stations in Kyonggi Bay during the summer seasons in 1970 and 1971. The concentrations of dissolved oxygen, total hardness, ammonia, nitrate, nitrite, phosphate, chemical oxygen demand, salinity, biochemical oxygen demand, coliform bacteria and facel coli were examined together with the measurement of pH and transparency. The relationship between the extent of pollution and the distance from the Inchon Bay also was examined. The concentrations of biochemical oxygen demand, chemical oxygen demand, ammonia, nitrate, nitrite, phosphate, coliform bacteria nad fecal coli were all highest at station 1, and lowest at station 4. Values were somewhat higher at low tide level in general. On the contrary, dissolved oxygen concentration was highest at station 4 and lowest at station 1. The highest and lowest values of biochemical oxygen demand were 10.88 ppm at station 1 and 0.27 ppm at station 4. The chemical oxygen demand concentrations at station 1 and 4 were 1.90 ppm and 0.63 ppm. Ammonia concnetration at station 1 was 0.43 pp, and was nearly 5 times as much as that at station 4. The values were $2.45{\times}10^{-4}$ ppm at station 1, and $6{\times}10^{-4}$ ppm at station 4. Nitrite concnetration at station 1 was $3{\times}10^{-4}$ppm and was the highest, while the lowest was $9.45{\times}10^{-5}$ ppm at station 4. Phosphate value at station 1 too was the highest and was about 4 times as much as that at station 4. Coliform bacteria were most abundant at station 1, and were counted to be 1.$1.7{\times}10^{-5}$cells/ml. At station 4, the number greatly reduced to 8 * 10$^{2}$ cells/ml. The number of fecal coli at station 1 was $2{\times}10^{-4}$ cells/ml. But at station 4, no fecal coli was detected at high tide level. At low tide level, 3 cells/ml were counted at station 4. In all of these, the highest data were obtained at low tide level, while most of the lowest value, at high tide level. Generally, values at statio 1 were 3-5 times as much as those at station 4. Concentration of dissolved oxygen at station 1 was 0.366 mg-atoms/1 and was the lowest. The highest value was 0.420 mg-atoms/1 and was recorded at station 4. The highest at station 4, which certainly were believed to be the result of the dilution by the fresh water of the Han river flowing into the Inchon Bay. As we can see from the data above, the extent of pollution was highest at station 1, the nearest from Inchon harbor, and lowest at station 4, where is the farthest, Station 1 and 2 were proved to be much polluted, but station 3 and 4, not.

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Effects of Leachate during Vegetable Waste Composting using Rotary Drum Composter

  • Varma, V. Sudharsan;Kalamdhad, Ajay S.
    • Environmental Engineering Research
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    • v.19 no.1
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    • pp.67-73
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    • 2014
  • In India, disposal of vegetable market waste along with municipal solid waste in landfills or dumpsites is creating much nuisance in terms of odor nuisance, leachate production, and greenhouse gas emission into the atmosphere. Therefore, vegetable waste with high biodegradable and nutrient content is composted in a 550-L batch scale rotary drum composter to study the degradation process and its compost properties for its potential reuse as high quality compost. A total 150 kg of working volume was fixed for composting studies with two different ratios, trial A (6:3:1) of C/N 24 and trial B (8:1:1) of C/N 30, respectively. A maximum of $63.5^{\circ}C$ and $61.2^{\circ}C$ was observed in trials A and B; an average of $55^{\circ}C$ for more than 5 days, which helped in the degradation of organic matter and reduction of total and fecal coliform. The temperature dropped suddenly after the thermophilic stage in trial B, and leachate was observed due to insufficient amount of bulking agent. Mesophilic bacteria dominated during the initial stages of composting, and reduced considerably during the thermophilic stage. During the thermophilic stage, the rise in spore-forming organisms, including spore-forming bacteria, fungi, actinomycetes and streptomycetes, increased and these were predominant until the end of the composting process. By examination, it was observed that moisture and leachate production had adverse effects on the compost parameters with higher loss of micronutrients and heavy metals.

Feasibility of Reclaimed Wastewater and Waste Nutrient Solution for Crop Production in Korea

  • Choi, Bong-Su;Lee, Sang-Soo;Awad, Yasser M.;Ok, Yong-Sik
    • Korean Journal of Environmental Agriculture
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    • v.30 no.2
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    • pp.118-124
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    • 2011
  • BACKGROUND: Development of water recycle technologies is important for human health and sustainable agriculture. However, few studies have been conducted to examine the purification methods or the water quality of reclaimed wastewater in Korea. METHODS AND RESULTS: In this study, the different wastewaters including reclaimed wastewater and waste nutrient solution (NS) were evaluated. The changes of water quality in reclaimed wastewater and NS were determined using ultraviolet (UV) treatment and sand filtration with charcoal. Our results showed that one of the most critical limitations of reusing wastewater was the presence of harmful pathogens that possibly cause human health risks. CONCLUSION(s): This study suggests that the application of UV treatment or combined with sand filtration on reclaimed wastewater and waste NS effectively removes the total coliform bacteria below the harmful or acceptable level. For future studies, a long-term field monitoring after applying reclaimed wastewater or NS is needed.

Discharge of the Pollutants from Rice Paddies during the Period of Cultivation (경작기 농경배수에 의한 오염물질의 배출)

  • An, Ik-Sung;Kim, Youngchul;Lee, Dong-Ryul
    • Journal of Korean Society on Water Environment
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    • v.23 no.2
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    • pp.266-273
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    • 2007
  • In this study, discharge of the pollutants from the rice paddies during cultivation and its pattern were investigated. The pH in the returned and rainfall water from ten different paddies was between 6.9 and 7.4, which is not associated with the cultivation time and farming style of individual paddy. TN and TP concentrations were highest in the beginning of first top dressing (fertilization), which indicates that proper top dressing and returned water management is crucial to reduce their discharge loads. In particular, TN concentration was more or less constant until tillering stage. This is thought to be due to the runoff of soil-adsorbed nitrogen and compost. Average SS concentration in the returned and rainfall water was 28 mg/L, and organics was predominantly soluble form, and highest COD 25 mg/L during transplanting, lowest 11.3 mg/L and average 11.3 mg/L. Density of TC (total coliform bacteria) was from 1000/100 mL to 114,000/100 mL and FC (fecal coliform) was 5 to 16% of TC density.