• Title/Summary/Keyword: Total Phenolic Contents

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Study on the Antioxidant Effect and Total Phenolics Content in Rosaceae Plant Stem (장미과 식물 줄기의 항산화 효과와 총 페놀류 함량에 관한 연구)

  • Lee, Jun-Young;You, Ju-Han;Kim, Sang-Wook
    • Journal of Environmental Science International
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    • v.23 no.12
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    • pp.2129-2134
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    • 2014
  • The antioxidant activities and total phenolics of four Rosaceae species Pourthiaea villosa (Thunb.) Decne, Sorbus commixta Hedlund, Sorbaria sorbifolia var. stellipila Maxim and Pyrus pyrifolia (Burm.f.) Nakai were determined. Phenolic content (polyphenol and flavonoid), radical scavenging activities [2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylnezthiazoline-6-sulfoic acid) (ABTS)] and ferrous ion chelating effect were evaluated. Total polyphenol and flavonoid contents were highest in Pourthiaea villosa (Thunb.) Decne and lowest in Pyrus pyrifolia (Burm.f.) Nakai. Phenolic contents of Pourthiaea villosa (Thunb.) Decne was $331.45{\pm}7.78$ and $90.4{\pm}3.5mg{\cdot}g^{-1}$. DPPH and ABTS radical scavenging activities were found to be lowest in Sorbus commixta Hedlund whereas Sorbaria sorbifolia var. stellipila Maxim and Pourthiaea villosa (Thunb.) Decne showed relatively good DPPH and ABTS radical scavenging activities. Ferrous ion chelating effect was highest in Pyrus pyrifolia (Burm.f.) Nakai ($1.05{\pm}0.04mg{\cdot}ml^{-1}$) and lowest in Sorbus commixta Hundlund ($4.22{\pm}0.71mg{\cdot}ml^{-1}$).

Quality Characteristics and Antioxidant Activity of Cookies with Stevia Powder (스테비아 분말을 첨가한 쿠키의 품질특성과 항산화 활성)

  • Yoo, Seung-Seok;Hong, Yeo-Joo
    • Korean journal of food and cookery science
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    • v.28 no.6
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    • pp.665-673
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    • 2012
  • The purpose of this study was to propose optimal mixing proportion by adding stevia in powder form, which are low caloric and high intensity sweetener to cookies, and to determine the characteristic of product. It reduced the average sugar content of cookies to 50%, processed stevia powder, add them in 2, 4, 6, and 8% to make cookies and measured various product characteristics, including anti-oxidant activity. The antioxidant activity was highly correlated with the total phenolic composition of stevia cookies (r=0.967). Total phenolic contents and DPPH free radical scavenging activity of cookies significantly increased with increasing stevia powder. The bulk density of the dough, spread factor and the hardness, total polyphenol contents and DPPH free radical scavenging activity of cookies significantly increased with increasing stevia powder, while the pH of the dough, and L values of the cookies decreased with increasing stevia powder content (p<0.001). Additionally, the consumer acceptability scores for the 4% stevia cookie groups ranked significantly (p<0.001) higher than those of the other groups in color, taste, flavor, sweetness and overall preference. All the take together, the results of this study suggest that stevia powder is a good ingredient for increasing the consumer acceptability and the functionality of cookies.

Evaluation of Antioxidant Activity of the Extracts from the Aerial parts of Cnidium officinale Makino (천궁(Cnidium officinale Makino) 지상부 추출물의 항산화 활성 평가)

  • Oh, Yeong-Ji;Seo, Ha-Rim;Choi, Yoo-Mi;Jung, Dong-Sun
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.6
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    • pp.373-378
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    • 2010
  • In order to obtain basic data for utilization of the aerial parts of Cnidium officinale Makino (APCO), the antioxidant properties of the aerial parts and rhizomes of C. officinale were measured using DPPH and ABTS radicals, and nitrite scavenging assays. The ethyl acetate (EA) fraction prepared from the aerial parts of APCO showed the strongest antioxidant activities, and contained high level of total phenolic compounds (325.81 mgTE/g) and flavonoids (259.16 mgRE/g). The concentrations for 50% reductions ($RC_{50}$) values of the DPPH and ABTS radicals, and nitrite by the EA fraction of APCO were $11.27\;{\mu}g/m{\ell}$, $14.34\;{\mu}g/m{\ell}$, and $10.26\;{\mu}g/m{\ell}$, respectively. APCO exhibited approximately 3-9 times higher antioxidant activity than rhizomes of C. officinale. The antioxidant capacities of APCO were positively correlated with its total phenolic contents. Therefore, it was concluded that the aerial parts of C. officinale can be a useful and cost-effective source of natural antioxidant for food or cosmetics.

Antioxidant Activity of the Various Extracts from Different Parts of Lotus (Nelumbo nucifera Gaertner)

  • Choi, Hae-Yeon;Jung, Kyung-Hee;Shin, Han-Seung
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.1051-1054
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    • 2009
  • This study was conducted to investigate the antioxidant activity of the extracts of lotus (Nelumbo nucifera Gaertner). The total phenolic contents in leaf, stem, and root were 165, 74, and 30 tannic acid equivalent mg/g of dried extract or fraction respectively. The butanol and ethylacetate fractions of lotus parts showed higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than other fractions. 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity also showed the similar result as the DPPH radical scavenging activity. The antioxidative capacity of the ethylacetate fraction was the highest among fractions and its fraction showed higher contents of total polyphenol.

Phenolic Acid Contents and ROS Scavenging Activity of Dandelion(Taraxacum officinale) (민들레(Taraxacum officinale) 잎과 뿌리의 페놀산 조성 및 활성산소 소거활성)

  • Kim, Young-Chan;Rho, Jeong-Hae;Kim, Kyung-Tack;Cho, Chang-Won;Rhee, Young-Kyung;Choi, Ung-Kyu
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.325-331
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    • 2008
  • The propose of this study was to investigate the antioxidant activity of 80% ethanol extracts and various solvent fractions of dandelion (Taraxacmn officinale) leaves and roots, Total phenolics and phenolic acid contents were also examined. The total phenol content of leaves and roots were $7.9{\pm}0.4%$ and $9.4{\pm}0.3%$ respectively. Eight phenolic acids were separated by GC, among which caffeic acid (113.7 mg%)and m-coumaric acid (152.6 mg) were the dominant phenolic acids in leaves and roots, respectively. Amongst solvent functions of leaves and roots, the ethyl acetate fraction showed the strongest radical scavenging activity. A strong correlation was found between total phenol content and electron-donating ability, and ABTS radical scavenging activity showed a similar trend as electron-donating ability. Hydroxyl-radical-scavenging activity and lipid peroxidation were significantly higher in the ethyl acetate fraction than other factions. In particular, the SOD-like activity was highest (43.6%) in the ethyl acetate fraction of dandelion leaves, and was higher than that of trolox. Thus, the ethyl acetate fraction of dandelion leaves exhibited significant phenol content, antioxidant activity, and free-radical-scavenging effects.

Composition and Contents of Free Amino Acids and Phenolic Acid and Flavonoids of Imperata cylindrica Beauvois var. koenigii Root extracts (백모근 추출물의 유리아미노산 및 Phenolic acid와 Flavonoids 조성 및 함량)

  • Lee, Soon-suk;Lee, Keun-Kwang
    • Journal of Digital Convergence
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    • v.18 no.7
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    • pp.397-403
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    • 2020
  • This study was carried out to investigate the free amino acid and derivatives and phenolic acid and flavonoid compositions and contents of hot water and 95% ethanol extracts of Imperata cylindrica Beauvois var. koenigii root. The purpose of this study is to obtain basic data and to confirm their applicability as functional cosmetic and functional food ingredients on extracts. 15 kinds and 9 kinds of free amino acids were detected in both extracts, respectively. 5 kinds and 4 kinds of protein free-amino acid derivatives were detected in both extracts, respectively. Total phenol contents were 116.50 ± 0.06 and 140.10 ± 0.04 mg% in both extracts, respectively. The total flavonoid contents were 31.80 ± 0.03 and 43.90 ± 0.05 mg% in both extracts, respectively. 5 kinds and 6 kinds of phenolic acids were detected in both extracts, respectively. In the case of flavonoid analysis, no flavonoid compound was detected in the hot water extract while taxifolin was identified in the 95% ethanol extract. Based on the above results, the hot water and 95% ethanol extracts of I. cylindrica Beauvois var. koenigii root are rich in free amino acids, amino acid derivatives, phenolic acids and flavonoids which confirm their potential for applications in cosmeceuticals, nutricosmetics formulations and functional foods.

Enhancement of Antioxidant Activity, Total Phenolic and Flavonoid Content of Saccharina japonica by Submerged Fermentation with Aspergillus oryzae (Aspergillus oryzae 심부배양에 의한 다시마의 항산화능, 총페놀 및 플라보노이드 함량의 증대)

  • Rafiquzzaman, S.M.;Kong, In-Soo;Kim, Jin-Man
    • KSBB Journal
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    • v.30 no.1
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    • pp.27-32
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    • 2015
  • The current investigation was carried out to explore the possibility of submerged fermentation of Saccharina japonica as sole substrate using Aspergillus oryzae. In this study we used 2% S. japonica powder as fermentation media for A. oryzae. Fermentation period was optimized by monitoring the fermented sample at regular intervals for a period of 7 days. Results found that a fermentation period of 5 days was effective with maximum desirable characteristics such as total sugar, total phenolic and flavonoid contents. Under optimum fermentation period, fermented extracts showed enhanced antioxidant activity as determined by different assays such DPPH radical scavenging, ABTS scavenging and phosphomolydenum assay. This study provides the information for the enhancement of bioactive molecules in an eco-friendly manner and also paves way towards the development of wide range of seaweed-based functional foods.

Comparison of the Contents of Phenolic Compounds of Sea Buckthorn(Hippophae rhamnoides) Cultivated in Korea and Mongolia (비타민 나무(사극)의 페놀성 성분 분석)

  • Lee, Sun-A;Jo, Hee-Kyung;Cho, Soon-Hyun;Ko, Sung-Kwon
    • Korean Journal of Pharmacognosy
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    • v.41 no.4
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    • pp.308-312
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    • 2010
  • The purpose of this research is to provide basic informations to discriminate between sea buckthorn (Hippophae rhamnoides) cultivated in the Republic of Korea and Mongolia. The phenolic compounds of sea buckthorn, were measured by the HPLC analysis. Catechin, rutin, quercetin, isorhamnetin were found in methanol extracts of sea buckthorn. Total phenolic compound of Korean sea buckthorn leaves (1.852%) was about five times higher than those of Mongolian sea buckthorn berries (0.338%). As a result, the order of the total phenolic compound and Catechin content was 1) sea buckthorn leaves, 2) sea buckthorn stems, 3) sea buckthorn roots, and 4) sea buckthorn berries. Statistically no big differences in levels of phenolic compounds were consistently found in sea buckthorn cultivated in the Republic of Korea and Mongolia investigated in this work.

The Comparison of Phenolic Compounds by Various Sections of Acanthopanacis Caulis (오갈피나무속 식물 줄기의 부위별 페놀성 성분 비교)

  • Lee, Jae Bum;Yun, Gee Young;Yook, Chang Soo;Ko, Sung Kwon
    • Korean Journal of Pharmacognosy
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    • v.49 no.4
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    • pp.341-348
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    • 2018
  • This study was carried out to obtain the basic informations for phenolic compounds by various sections of Acanthopanacis Caulis. The phenolic compounds of Korean Acanthopanacis bark and lignum (Acanthopanax divaricatus var. albeofructus, Acanthopanax koreanum) were measured by the HPLC analysis. The content of total phenolic components of Acanthopanax koreanum bark (1.532%) was about 9.9 times higher than that of Acanthopanax koreanum lignum (0.155%). And also, The content of total phenolic components of Acanthopanax divaricatus var. albeofructus bark (0.420%) was about 2.8 times higher than that of Acanthopanax divaricatus var. albeofructus lignum (0.149%). Eleutheroside E, a functional ingredient of Acanthopanacis Caulis, showed 3.6 times higher contents of Acanthopanax koreanum bark (0.144%) than Acanthopanax koreanum lignum (0.040%). In the case of Acanthopanax divaricatus var. albeofructus bark (0.129%), the content of the eleutheroside E was 1.7 times higher than that of the Acanthopanax divaricatus var. albeofructus lignum (0.074%).

Properties and Antioxidative Activities of Phenolic Acid Concentrates of Rice Bran (미강 페놀산 농축물의 특성 및 항산화 활성)

  • Jung, Eun-Hee;Hwang, In-Kyeong;Ha, Tae-Youl
    • Korean Journal of Food Science and Technology
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    • v.42 no.5
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    • pp.593-597
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    • 2010
  • This study investigated the properties and antioxidative activities of phenolic acid concentrates of rice bran. Rice bran contains bioactive substances such as phenolic compounds, which can provide health benefits as natural antioxidants. This study examined how levels of phenolic acids can be obtained efficiently through various extraction methods. The extractions of defatted rice bran were followed by using ethylacetate (RBE-I), ethylacetate after alkaline hydrolysis (RBE-II), and 80% methanol (RBE-III). For all extracts, yields (%), total polyphenol contents (TPC), various phenolic acids and antioxidative activities were estimated. RBE-II had the highest total polyphenol contents (526.72 mg/100 g rice bran) and showed high antioxidative activity (74.7%). To concentrate the phenolic acids, RBE-II was passed through Sep-pak $C_{18}$ Vac cartridge and F1-RBE-II was collected by the elution of 50% methanol. The total phenolic content of F1-RBE-II (736.8 mg/100 g rice bran) was higher than that of RBE-II (367.1 mg/100 g rice bran), and the ratios of ferulic acid (73%) and sinapic acid (14%) increased. As RBE-II was analysed by HPLC, 6 different phenolic acids were found via chromatography, whereas F1-RBE-II showed 5 different peaks and the major phenolic acid was identified as ferulic acid. The ABTS radical scavenging activity of F1-RBE-II was the highest among the rice bran extracts. In a ${\beta}$-carotene-linoleic acid model system, linoleic acid oxidation was reduced by F1-RBE-II (73%) and RBE-II (35%).