• Asian-Australasian Journal of Animal Sciences
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• v.33 no.3
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• pp.446-455
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• 2020

Objective: Our recent series of laboratory- and large-scale experiments confirmed that under aerobic and anaerobic conditions, sodium metabisulfite (SMB) was effective in preserving nutrients and antioxidant capacity of highly perishable fruit and vegetable discards (FVD). Hence, the purpose of this study was to examine how partial inclusion of SMB-treated FVD in total mixed ration (TMR) influences in vitro ruminal fermentation, whole-tract digestibility, nitrogen metabolism, blood metabolites, and voluntary feed intake of sheep. Methods: The FVD were mixed thoroughly with 6 g SMB/kg wet biomass and kept outdoors under aerobic conditions for 7 days. Four TMRs including four levels of SMB-treated FVD (as-fed basis) at 0%, 10%, 20%, and 30% (equaling to 0%, 1.9%, 3.8%, and 5.7% on dry matter basis, respectively), were prepared as replacement for corn grain. The ruminal fermentation metabolites were studied using an in vitro gas production test. Four mature male Corriedale sheep were assigned at random to the 4 diets for two separate sub-experiments; i) digestibility trial with four 21-d periods, and ii) voluntary feed intake trial with four 28-d periods. Results: Inclusion of SMB-treated FVD in the TMR tended to quadratically increase partitioning factor. No effect was seen on total-tract digestibility of organic matter, ether extract, crude protein, and acid detergent fiber, except for neutral detergent fiber digestibility that tended to linearly increase with increasing SMB-treated FVD in the TMR. The progressive increase of FVD preserved with SMB in the diet had no effect on nitrogen metabolism. Treatment had no effect on serum antioxidant capacity and blood metabolites assayed. Voluntary feed intake was not impaired by inclusion of SMB-treated FVD in the TMR. Conclusion: It appears that FVD preserved with SMB can be safely incorporated into TMR as replacement of corn grain without impairment of nutrient metabolism and feed intake.

• Journal of Biosystems Engineering
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• v.43 no.2
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• pp.119-127
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• 2018

Purpose: Fermented feedstuffs have been found to improve productivity, reduce manure odor, and increase immunity. However, because there is not a commercialized pelletizing system for fermented total mixed ration (TMR) for pig feeding in Korea, a pelletizing system using TMR fermented feed was developed. Methods: The particle size, density, and volumetric density of the TMR feeds used in the test were measured. The pellet durability index (PDI, %) value of the pelletized TMR feed based on its moisture content, and the amount of pellet production based on the rotation speed of the compression roller were measured. Results: The test materials, TMR1 and TMR2, were approximately compressed to 387 kg/m3 with 18.2% (w.b.) and 544 kg/m3 with 22.2% (w.b.), respectively. Throughout this pellet molding test, the moisture content from 15 to 20% (w.b.) of mixture feedstuffs, including fermented forage, could be used for pellet molding. Based on the results, a small-scale pellet molding system of fermented TMR was designed and manufactured for pig farms. As rotation speed increased, the throughput increased, whereas the moisture content decreased by approximately 2% (w.b.) because of pellet molding. The best yield of pellets with 94.2% PDI was of 536 kg/h at 135 rpm rotation speed. Conclusions: Although the throughput of the prototype increased as the rotation speed increased, it was difficult to operate because of the greater noise and the lower PDI (%) at the higher rotation speed of the pellet molding rotor. It was found that the best production of pellets using the prototype was 536 kg/h having a PDI of 94.2% or more at a rotation speed of 135 rpm.

• Journal of Animal Environmental Science
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• v.17 no.sup
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• pp.7-20
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• 2011

This study was conducted to analyze the mixing ratio of raw feed materials for the methane mitigation and also to identify the minimum rearing density for improving the productivity of beef calves as eco-friendly fodder. Raw materials used in this study for the formulation of feed for methane reduction were crushed corn and alfalfa along with other 21 species. In addition, to investigate the appropriate rearing density, 12 Hanwoo calves with average weight of 150 kg was selected and experiment was conducted for four months. Methane gas emission (Bo) is about 3-4 times less in TMR 4 compared to TMR 1, 2 and 3. Feed price calculated for TMR 4 ration was also affordable. In addition, all TMRs showed a normal ruminal pH. Disappearance rate was observed to be lower in TMR 4 as compared to TMR 1, 2 and 3, but methane production decreased by 24 to 37%. The result showed improved total body weight, average daily gain and feed conversion ratio in rearing low-density ($18m^2/head$), and general treatment ($9m^2/head$) compared to overcrowding treatment ($6m^2/head$). In addition, blood components (total protein, glucose, AST, ALT and GGT factors) involved in health and disease treatments and health-related nutrition metabolism are lower in the low-density and general treatment compared to the high density treatment. Postural development (development of body size) i.e., weight, height and width significantly increased in the low and general density treatment compared to high density treatment. Especially excellent improvement was observed in low-density treatment than the general treatment. Moisture content, colonic bacteria and coccidium are higher in low and high density treatments than in the general treatment. The adequacy for beef rearing density is considered to be more desirable in an area more than $6m^2/head$. In conclusion, present study suggests that possibility of methane reduction through adjusting mixed feed ration. Also, rearing density is also an important factor in the growth and development of beef calves.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.253-262
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• 2009

The present study was conducted to develop a rapid and accurate method of evaluating chemical composition of total mixed ration (TMR) for dairy cows using near infrared reflectance spectroscopy (NIRS). A total of 253 TMR samples were collected from TMR manufacturers and dairy farms in Korea. Prior to NIR analysis, TMR samples were dried at $65^{\circ}C$ for 48 hour and then ground to 2 mm size. The samples were scanned at 2 nm interval over the wavelength range of 400-2500 nm on a FOSS-NIR Systems Model 6500. The values obtained by NIR analysis and conventional chemical methods were compared. Generally, the relationship between chemical analysis and NIR analysis was linear: $R^2$ and standard error of calibration (SEC) were 0.701 (SEC 0.407), 0.965 (SEC 0.315), 0.796 (SEC 0.406), 0.889 (SEC 0.987), 0.894 (SEC 0.311), 0.933 (SEC 0.885) and 0.889 (SEC 1.490) for moisture, crude protein, ether extract, crude fiber, crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF), respectively. In addition, the standard error of prediction (SEP) value was 0.371, 0.290, 0.321, 0.380, 0.960, 0.859 and 1.446 for moisture, crude protein, ether extract, crude fiber, crude ash, ADF and NDF, respectively. The results of the present study showed that the NIR analysis for unknown TMR samples would be relatively accurate. Use of the developed NIR calibration curve can obtain fast and reliable data on chemical composition of TMR. Collection and analysis of more TMR samples will increase accuracy and precision of NIR analysis to TMR.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.3
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• pp.197-208
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• 2007

This study was carried out to investigate effects of increasing moisture content with or without supplementing inoculant (Lactobacillus plantarum) in TMR (total mixed ration) on its feed value. In case of exposing TMR to air, the lower the moisture level of TMR was, the less its apparent condition was changed. The time of spreading of molds tended to be faster in TMR with the higher moisture level. And also the odor was influenced by moisture content and inoculant supplement that is, sour odor was smelled from 24 hour after exposing TMR containing 35% and 50% moisture to air, but TMR supplemented with inoculant had sweet odor. The inner temperature of TMR containing 35% and 50% moisture without inoculant tended to increase continually after the lapse of 6 hours when the TMR was exposed to air. The inner temperature of TMR containing 35% moisture with inoculant tended to increase dramatically after the lapse of 48 hours when exposed to air, but that of TMR containing 50% moisture with inoculant tended to increase after 6 hours. The pH of TMR containing 15% was consistent regardless of exposing time to air, but that of TMR containing 35% and 50% moisture considerably increased after 12 and 24 hours, respectively. The concentration of $NH_3-N$ of TMR supplemented with inoculant was increased from 6 hours after exposure to air, while that or TMR without inoculant increased from 12 hours. Nutrient content or TMR tended to be increased with the increase of exposing time to air and storage time under sealed condition.

• Journal of Life Science
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• v.19 no.2
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• pp.241-248
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• 2009

In order to investigate the effects of synbiotics on change of chemical composition and fermentation characteristics of total mixed ration (TMR), eight TMRs fermented by synbiotics composing the anaerobic microbes (bacteria, yeast, mold) were alloted to the experimental treatments. Treatments were composed of untreated synbiotics(US), bacterial synbiotics (BS), yeast synbiotics (YS), mold synbiotics (MS), bacterial and mold synbiotics (BMS), yeast and mold synbiotics (YMS), bacterial and yeast synbiotics (BYS), and bacterial, yeast and mold synbiotics (BYMS). After 7 days of anaerobic fermentation, fermented-TMRs were exposed to air during 1, 3, 5, 7, 14 and 21 days. One hundred forty four (8 treatments ${\times}$ 6 exposing days ${\times}$ 3 replications) fermented- TMRs were manufactured by vinyl bag sized of 43 cm by 58 cm. The results obtained were as follows. Moisture contents of the fermented TMRs anaerobically ranged from 41% to 45%, and was similar to those of basal TMRs. As results of anaerobic fermentation, the concentration of crude protein was decreased by 11.7% to 14.8% in the untreated sample, while was rather increased by 11% when the TMR was fermented with BMYS. And also BMYS treatment showed decreases by 32% for crude fiber, 15.5% for NDF and 26.1% for ADF. Internal temperature of fermented-TMRs was highest at 7 day of exposing in the air. The pH of fermented-TMR juice was significant difference betweentreatments after 7 day of exposing in air, and that of BMS was highest at 14 day after exposing in air (P<0.05). Acid buffering capacity was increased in proportion to the exposing day of TMR, and peaked at 7 or 14 days after exposing. Ammonia concentration of fermented-TMRs was highest at 5 day after exposing in the air. Individual volatile fatty acid of fermented-TMR juice was very low level in all treatments. Although BMYS treatment to TMR inclined to increase in crude protein and decrease in fibers, but there were no positive effects on the fermentation characteristics after exposing in the air by supplementation of anaerobic synbiotics to TMR.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.58-66
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• 2013

This study was conducted in order to develop slaughterhouse rumen content (SRC) as a potential feed additive. The moisture content of SRC can reach 80%, and therefore an appropriate dewatering process is required before it can be used. In this study, the effects of heating temperature, heating time, and filler content during the dewatering process on the activity of various enzymes in SRC were investigated. The Box-Behnken experimental design was employed, involving a total of 45 experimental runs, consisting of three variables (heating time, heating temperature, and filler content) with three levels per variable (12, 30 and 48 hr; 60, 75 and $90^{\circ}C$; 12, 22.5 and 33% for heating time, heating temperature, and filler content, respectively). For enzyme activities, xylanase, cellulase, and amylase were examined, and the results were subjected to an analysis of variance. Heating time, heating temperature and filler content had significant effects on the activity of each enzyme (p<0.05). Cellulase and amylase activities decreased (p<0.05) at elevated heating temperatures, whereas xylanase was reasonably stable around $90^{\circ}C$. The activities of all enzymes decreased (p<0.05) with increased heating time. Optimum filler contents for xylanase, cellulase, and amylase activities were 22.5, 12 and 33%, respectively. However, optimum conditions for all variables that simultaneously maximize the activity of all three enzymes could not be ascertained in this study. Nevertheless, the results from the current study can be useful as basic information for the development of SRC as a feed additive enriched with improved major enzymes for livestock feed digestion.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.5
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• pp.578-583
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• 2007

The purpose of this research was to study the effects of feeding TMR (total mixed ration) dietary citrus by-products on nutritional composition and palatability of Hanwoo loin. Samples for the experiment consisted of Hanwoo loin that was not fed citrus by-products (TMR-0) and Hanwoo loin that was fed citrus by-products during fattening periods (TMR-1). The control (TMR-0) Hanwoo loin was fed by general practical feeding (roughages and concentrates were fed separately); the TMR-1 Hanwoo loin was fed identically to TMR-0 until 17 months yearling, but fed citrus by-products for 10 months after then. The levels of the moisture, crude protein, crude ash, cholesterol, Mg, K, vitamin $B_1$, vitamin $B_2$, total amino acids ($17.81{\sim}17.98%$), and total free amino acids ($139.46{\sim}149.15mg/100g$) were not significantly different between TMR-0 and TMR-1. The contents of the crude fat, Ca, and Na of TMR-1 were greater than those of TMR-0 (p<0.05). Oleic acid ($45.01{\sim}49.38%$) was the most abundant anions unsaturated fatty acid while palmitic acid ($26.52{\sim}28.39%$) was tile most abundant saturated fatty acid in both groups. In case of sensory scores, taste, flavor, and juiciness were not significantly different between TMR-0 and TMR-1. However, tenderness and palatability preference of TMR-1 were higher than those of TMR-0 (p<0.05).

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.4
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• pp.267-272
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• 2021

This experiment was conducted to the purpose of evaluating the effect of feeding on Korean Native Cattle to expand the production and utilization of whole crop rice (WCR). TMR (Total mixed ration) feed was prepared by using WCR produced in Pyeongtaek, Gyeonggi-do, and the growth capacity and meat quality characteristics of 16 Korean Native Cattle raised up to 29 months of age were investigated. The produced WCR silage for feed had a moisture content of 64.02% and a crude protein content of 7.54%, and was blended with about 45% during the growing season, and lowered to 35, 15 and 9%, respectively, in the fattening period (early, middle and late stages). The body weight of the WCR-TMR feeding group was significantly higher than that of the control in the middle and late fattening stage, and at the end (29 months of age), the control group was 631 kg/head, but the WCR-TMR feeding group was 647 kg/head, which was higher. The average daily gain was significantly higher in the WCR-TMR feeding group in the growing and early fattening period, and there was no difference in the mid- and late fattening period. In the whole period, 0.71 vs 0.75 kg/head/day, WCR-TMR feeding group was high. In terms of meat quantity, the back fat thickness of the control group (11.7 mm) was significantly thicker than that of the WCR-TMR fed group (9.3 mm) (P<0.05). There was no difference in Rib eye area, Carcass weight and Meat yield index (P>0.05). In terms of meat quality, the Marbling score was higher in the WCR-TMR feeding group (P<0.05), and there were no significant differences in Meat color, Fat color, Texture and Maturity. Considering the above results, TMR feeding mainly on whole crop rice silage for feed improved the productivity of livestock, but there was no significant difference in meat quality. Therefore, it is judged that it is necessary to produce and use the whole crop rice for feed in countries with poor forage conditions.