• 대한기계학회논문집A
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• 제41권5호
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• pp.427-433
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• 2017

고강도 재료나 열전도성이 낮은 재료의 밀링 가공시 가공 부위에서 발생하는 높은 마찰열로 인해 공구 파손 등이 심각하게 발생할 수 있으므로 절삭유 공급이 매우 중요하다. 기존의 절삭유 공급 방식은 수동식 관절 구조를 활용한 방법으로 과도한 양을 분사함에도 불구하고 효과적인 결과를 얻기 힘들었다. 또한, 작업 중 비산으로 인해 작업장 환경에도 큰 악영향을 미쳤다. 이에 본 연구에서는 자동으로 분사 위치 조절이 가능한 장치에 기반하여 절삭유 분사위치에 따른 STS316L 의 밀링가공 특성을 분석하였다. 그 결과, 절삭유 공급 위치 변화에 따라 공구 마모와 표면 거칠기 변화가 관찰되었으며 이를 통해 제작된 장치의 효용성을 보였다.

• Ocean and Polar Research
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• 제33권2호
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• pp.127-133
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• 2011

The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide ($H_2O_2$); strong correlation between $H_2O_2$ concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 ${\mu}g/L$) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 춘계학술대회 논문집 전력기술부문
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• pp.278-282
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• 2001

The purpose of this study is to introduce a new spacer damper for the bundle transmission lines network. It has the special design, the main characteristics and advantage of this new kind of spacer damper. An Existing spacer damper with bolted clamps, although widely used, is a method of connection with certain disadvantage both as regards assembly on the conductor and in the course of time. Even if tightening torque is correctly applied by using bolt with share head or torque wrench during working time, the aeolian vibration could involve untightening during life time, so the cable can move into the Jaws and wire's breakage appear. To salve this problems, France, Japan and other countries had developed a spacer damper with an automatic system through many years. This new spacer damper is an original automatic clamping device (beltless) which does not require special tool for its installation. This device prevents clamp unlocking problems, ensures a simple installation and ensures a reliable-tightening during life time. Therefore, it is necessary to localize this boltless spacer damper with automate clamping device.

• 한국재료학회지
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• 제21권11호
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• pp.623-627
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• 2011

This is a study on the distribution of acoustic emission parameters during a burst test for a type-II CNG vehicle fuel tank. A resonant AE sensor with a central frequency of 150 kHz was attached to the composite materials in the center of the fuel tank. The pressure was increased from 30 to 100% of the expected burst pressure and was maintained for 10 minutes at each level. Damage at 70% of expected burst pressure occurred by various damage mechanisms including fiber breakage and delamination, while that of below 60% only occurred by matrix crack initiation and growth. The count, duration and rise time of the AE signal at 60% of the expected burst pressure are distributed below 500, 5000 ${\mu}s$ and 300 ${\mu}s$, respectively. Then, at above 70% they increased with pressure by superimposing of individual AE signal generated at a nearby place. These results confirmed that the analysis of the distribution of AE parameters is an effective tool for estimating damage of a CNG fuel tank.

• Design & Manufacturing
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• 제14권1호
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• pp.42-48
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• 2020

Titanium alloy has been in the spotlight as a core material in high-tech industries that require high strength and light weight because it has excellent strength and corrosion resistance and strength is higher than that of steel. Therefore, in various industries, existing steel products are intended to be replaced with titanium alloys. Titanium alloys can cause cutting tool breakage during cutting, and heat generated during cutting does not dissipate, accumulates in tools and workpieces, resulting in large wear and tear on thin workpieces. In addition, since titanium alloy is a metal with high chemical activity, the wear of the tool becomes more severe when the cutting speed is high, so machining of titanium bolt through cutting is very disadvantageous in terms of productivity. Therefore, the production of bolts using titanium alloys is being produced through a forging process to improve productivity and product quality. In this paper, hot forging molding analysis was performed on bolts used for fastening automobile parts using Ti-6Al-4V alloy, which is the most commonly used titanium alloy.

• 공업화학
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• 제31권2호
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• pp.220-225
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• 2020

모발은 여러 가지 아미노산들을 포함하는 단백질로 이루어져 있다. 자외선(UV)은 태양광선중에서 모발손상에 가장 큰 영향을 미치며 모발 노화에 주된 역할을 한다. 본 연구의 목적은 전자현미경(SEM), 공초점현미경(CLSM) 및 적외선 현미경분광법(IR micro spectroscopy)을 이용하여 정상모발에 UVB를 조사한 후 특징적인 형태학적 및 화학적 구조변화를 알아보는 것이다. 에너지 분산형 X선 분광기가 부착된 전자현미경은 자외선 조사모발의 표면이 정상모발과 비교했을 때 거칠고 높은 산소원소의 함량을 보였다. 형광 및 3차원 위상 이미지를 CLSM으로 분석한 결과 정상모발의 초록색 형광방출이 UVB 조사모발에 비해 매우 높았다. 또한 fluorescamine 형광 염색법을 통해 UVB 조사모발은 정상모발에 비해 펩타이드 결합의 파괴로 생성된 자유 아미노기가 많음을 확인할 수 있었다. UVB 조사모발의 강한 푸른색 형광은 아미노기의 함량이 높다는 것을 의미하며, 이는 CLSM에서도 관찰되었다. 따라서 fluorescamine은 UVB 조사모발에서 펩타이드 결합의 파괴를 관찰하는데 유용한 도구가 될 수 있다. 정상모발과 UVB 조사모발의 단면을 IR micro-spectroscopy를 통해 이미지 맵핑(mapping)한 결과, UVB 조사모발은 정상 모발에 비해 모발의 표면은 물론 내부에 걸쳐 디설파이드 결합(disulfide bond)의 산화가 일어나고 있음을 확인할 수 있었다. 이러한 분광학적 방법은 단독 또는 다른 분석법과 함께 모발화장품의 개발에 응용될 수 있을 것이다.

• 한국산학기술학회논문지
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• 제15권4호
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• pp.1871-1878
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• 2014

폴리메틸메타클릴레이트(PMMA)는 광 관련 부품제조에 있어 중요한 재료로 많이 사용되고 있다. 특히 TV, 모니터, 휴대폰 등에 쓰이고 있는 디스플레이의 도광판(Light Guide Panel)에 적용되고 있어 현재까지는 매우 큰 상업적인 수요가 있다. PMMA 도광판의 가공은 일반적으로 고속밀링에 의해 이루어지는데 부품에 대한 높은 정밀도 요구특성에도 불구하고 가공표면의 굴곡, 균열 등에 대한 불량이 적지 않게 발생하고 있다. 이러한 문제점을 해결하기 위하여 PMMA 진동을 부가하는 고속선삭의 적용이 시도되었으며 절삭공구에 진동을 부가하기 위한 장치와 최적 조건이 조사되었다. 도광판용 PMMA의 진동절삭은 일반적인 밀링가공법에 비해 절삭력의 현저한 감소, 열변형영역의 축소 등을 유도하였다. 가공면에 있어서는 가공방법이 엔드 밀링에서 단인공구를 사용하는 선삭으로 변경되었음에도 불구하고 진동절삭법에 의해 가공표면의 균일성이 크게 향상되어 가공면의 표준편차 $1.0{\sim}6.0{\mu}m$, 평균거칠기 $0.3{\mu}m$가 달성되었다.

• 한국정밀공학회지
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• 제29권12호
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• pp.1272-1278
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• 2012

The micro barrier rip array structures have been applied in a variety of areas including as privacy films, micro heat sinks, touch panel and optical waveguide. The increased aspect ratio (AR) of barrier rip array structures is required in order to increase the efficiency and performance of these products. There are several problems such as burr, defect of surface roughness and deformation and breakage of barrier rip structure with machining high-aspect ratio micro barrier rip array structure using orthogonal cutting method. It is essential to develop technological methods to solve these problems. The optimum machining conditions for machining micro barrier rip array structures having high-aspect ratio were determined according to lengths ($200{\mu}m$ and $600{\mu}m$) and shape angles ($2.89^{\circ}$ and $0^{\circ}$) of diamond tool, overlapped cutting depths ($5{\mu}m$ and $10{\mu}m$), feed rates (100 mm/s) and three machining processes. Based on the optimum machining conditions, micro barrier rib array structures having aspect ratio 30 was machined in this study.

• 산업공학
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• 제21권4호
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• pp.411-417
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• 2008

An impeller is a type of high-speed rotor that is used to compress or transfer fluid under high-speed and pressure at high temperatures. The impeller is composed of an axial hub and several blades attached along the hub. The weight and shape of an impeller must be balanced, because their imbalances can cause noise and vibration, which can lead to the breakage of the impeller blades during operation. Thus, the hub and blades of an impeller are commonly machined in a 5-axis NC machine to obtain qualified surfaces. The impeller machining strategy or process plan can not be easily obtained due to the complex, overlapped and twisted shapes of impeller blades. Skillful machining process planners may generate appropriate machining strategies based on their experiences and floor data. However, in practice most shop floor data for the impeller machining is not well-structured such that it does not effectively provide a process planner with information for machining strategies and/or process plans. This paper reports the development of a case-based machining strategy support system (CBMS) that employs case-based reasoning to obtain the machining strategy of an impeller by using the existing machining strategies of the shop floor. The CBMS generates impeller machining strategies through a stepwise reasoning process considering the similarity features between the blade shapes and machining regions. A case study is provided to demonstrate that CBMS can generate useful machining strategies facilitating process planners. The developed system can simulate the tool paths of impeller machining and runs on the web.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 1997년도 제20회 화학물질의 환경독성과 건강영향
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• pp.101-101
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• 1997

Comet assay, single cell gel electrophoresis has been known as useful, rapid, simple, visual, and sensitive technique for measuring the DNA breakage in mammalian ce1ls. For evaluation of DNA damage using comet assay, early studies reported a change in comet length and intensity with DNA damage using simple visual technique, such as fluorescence microscopy with eyespiece. In recent, some workers are observing and analyzing nucleotide of comets using quantitative fluorescence image analysis system to estimate 'tail moment', which is defined as the product of the tail length and the fraction of total DNA in tail. Our laboratory also adopted the image analysis software for qualification. In addition, many of the practical features of comet assay render it potentially attractive as useful tool for molecular toxicology and carcinogenesis, because the system is already showing considerable promise as rapid predictor in both in vitro and in vivo experimental designs. Recently, the comet assay becomes a attractive technique to study of apoptosis, because apoptotic fragmentation of nuclear DNA into nucleosomal sizes can be evaluated by the comet assay. So, we attempted to apply the comet assay to studying the effect of various stress on the apoptosis-sensitive cell lines. Particularly, focusing on the hyperthermic apoptosis, we could find that heat shock(44˚C for 60 minutes) was sufficient to induced apoptosis in these cell lines. But using the highly sensitive comet assay, we could not detect DNA breaks immediately after heat shock.