• Transactions of the Korean Society of Mechanical Engineers A
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• v.41 no.5
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• pp.427-433
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• 2017

In the case of high-strength or low thermal conductivity material milling, tool breakage occurs easily because of the high friction temperature. Therefore, the effectiveness of the coolant supply is very important for proper tool cooling. As the manually adjustable joint mechanism nozzle is generally used for coolant supply, the cooling efficiency is very low. It also has a bad influence on the workspace environment because of coolant scattering. In this study, the milling characteristics of STS316L were investigated according to the coolant spray position based on the automatic adjustable system. Tool wear and surface roughness were measured according to the coolant spray position. Through these experiments, the effectiveness of the fabricated system was explained.

• Ocean and Polar Research
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• v.33 no.2
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• pp.127-133
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• 2011

The comet assay, also called single-cell electrophoresis (SCGE) assay, is a potential sensitive monitoring tool for DNA damage in cells. The primary objective of this study was to use comet assay to ascertain if the blood cells of flounder (Pleuronichthys olivaceus) and muscle cells of clam (Saxidomus purpurata) are suitable for genotoxicity screening. This was achieved by initially exposing blood and muscle cells under in vitro conditions to the reference genotoxin hydrogen peroxide ($H_2O_2$); strong correlation between $H_2O_2$ concentration and comet values were found. Subsequently, the identification of DNA damage in isolated cells from flounder and clam was performed under in vivo exposure to benzo(a)pyrene (BaP) and tributyltin (TBT). Flounder and clam were exposed to different concentrations (1, 10, 50, 100 ${\mu}g/L$) of BaP or TBT for 4 days. Regardless of treated chemicals, blood cells of flounder were more prone to DNA breakage compared to muscle cells of clam. In conclusion, in vivo genotoxicity of BaP and TBT can be biomonitored using the comet assay. This study suggests that flounder and clam do show potential as mediums for monitoring genotoxic damage by comet assay.

• Proceedings of the KIEE Conference
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• 2001.05a
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• pp.278-282
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• 2001

The purpose of this study is to introduce a new spacer damper for the bundle transmission lines network. It has the special design, the main characteristics and advantage of this new kind of spacer damper. An Existing spacer damper with bolted clamps, although widely used, is a method of connection with certain disadvantage both as regards assembly on the conductor and in the course of time. Even if tightening torque is correctly applied by using bolt with share head or torque wrench during working time, the aeolian vibration could involve untightening during life time, so the cable can move into the Jaws and wire's breakage appear. To salve this problems, France, Japan and other countries had developed a spacer damper with an automatic system through many years. This new spacer damper is an original automatic clamping device (beltless) which does not require special tool for its installation. This device prevents clamp unlocking problems, ensures a simple installation and ensures a reliable-tightening during life time. Therefore, it is necessary to localize this boltless spacer damper with automate clamping device.

• Korean Journal of Materials Research
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• v.21 no.11
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• pp.623-627
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• 2011

This is a study on the distribution of acoustic emission parameters during a burst test for a type-II CNG vehicle fuel tank. A resonant AE sensor with a central frequency of 150 kHz was attached to the composite materials in the center of the fuel tank. The pressure was increased from 30 to 100% of the expected burst pressure and was maintained for 10 minutes at each level. Damage at 70% of expected burst pressure occurred by various damage mechanisms including fiber breakage and delamination, while that of below 60% only occurred by matrix crack initiation and growth. The count, duration and rise time of the AE signal at 60% of the expected burst pressure are distributed below 500, 5000 ${\mu}s$ and 300 ${\mu}s$, respectively. Then, at above 70% they increased with pressure by superimposing of individual AE signal generated at a nearby place. These results confirmed that the analysis of the distribution of AE parameters is an effective tool for estimating damage of a CNG fuel tank.

• Design & Manufacturing
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• v.14 no.1
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• pp.42-48
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• 2020

Titanium alloy has been in the spotlight as a core material in high-tech industries that require high strength and light weight because it has excellent strength and corrosion resistance and strength is higher than that of steel. Therefore, in various industries, existing steel products are intended to be replaced with titanium alloys. Titanium alloys can cause cutting tool breakage during cutting, and heat generated during cutting does not dissipate, accumulates in tools and workpieces, resulting in large wear and tear on thin workpieces. In addition, since titanium alloy is a metal with high chemical activity, the wear of the tool becomes more severe when the cutting speed is high, so machining of titanium bolt through cutting is very disadvantageous in terms of productivity. Therefore, the production of bolts using titanium alloys is being produced through a forging process to improve productivity and product quality. In this paper, hot forging molding analysis was performed on bolts used for fastening automobile parts using Ti-6Al-4V alloy, which is the most commonly used titanium alloy.

• Applied Chemistry for Engineering
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• v.31 no.2
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• pp.220-225
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• 2020

Hair is made of proteins containing various amino acids. Ultraviolet (UV) radiation is believed to be responsible for the most damaging effects of sunlight, and also plays an important role in hair aging. The purpose of this study was to investigate the changes in morphological and chemical structures after ultraviolet B (UVB) irradiation of human hair. The UVB-irradiated hair showed characteristic morphological and structural changes, compared to those of the normal hair. The result from a scanning electron microscope (SEM) equipped with an energy dispersive X-ray diffractometer (EDX) showed that the scale of UV-irradiated hair appeared to be rough and the amount of oxygen element was higher than that of the normal hair. Fluorescence and three dimensional (3D) topographical images were obtained by a confocal laser scanning microscope (CLSM). In 3D images, the green emission intensity of normal hair was much higher than that of fluorescing UVB-irradiated hair. The intensity of green emission reflects the intrinsic fluorescence of hair protein. Also, a fluorescent imaging method using fluorescamine reagent was used to identify the free amino groups resulting from a peptide bond breakage in UVB-irradiated hair. Strong blue fluorescence of UVB-irradiated hair, which indicates a very high level of amino groups, was observed by CLSM. Therefore, the fluorescamine as an extrinsic fluorescence could provide a useful tool to identify the peptide bond breakage in UVB-irradiated hair. Infrared image mapping was also employed to assess the cross-sections of normal and UVB-irradiated specimens to examine the oxidation of disulfide bonds. The degree of peak areas with strong absorbance for the disulfide mono-oxide was spread from the outside to the inside of hair. The spectroscopic techniques used alone, or in combination, launch new possibilities in the field of hair cosmetics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.4
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• pp.1871-1878
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• 2014

PMMA (Polymethyle-methacrylate) optical components have recently been increasingly used as one of the important part of the high precision equipments. This research presents comparatively the surface preparation of light incident plane, LIP (Light Incident Plane) of LGP (Light Guide Panel) by end milling, high speed shaping, and vibration assisted high speed shaping. From several experiments, the results show that the surface quality was improved in high speed shaping and the vibration assisted HSS show not only decreasing waviness and breakage also raising the straightness property. For applying high speed shaping and vibration assisted HSS, an additional tool post was developed and experimentally used.

• Journal of the Korean Society for Precision Engineering
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• v.29 no.12
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• pp.1272-1278
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• 2012

The micro barrier rip array structures have been applied in a variety of areas including as privacy films, micro heat sinks, touch panel and optical waveguide. The increased aspect ratio (AR) of barrier rip array structures is required in order to increase the efficiency and performance of these products. There are several problems such as burr, defect of surface roughness and deformation and breakage of barrier rip structure with machining high-aspect ratio micro barrier rip array structure using orthogonal cutting method. It is essential to develop technological methods to solve these problems. The optimum machining conditions for machining micro barrier rip array structures having high-aspect ratio were determined according to lengths ($200{\mu}m$ and $600{\mu}m$) and shape angles ($2.89^{\circ}$ and $0^{\circ}$) of diamond tool, overlapped cutting depths ($5{\mu}m$ and $10{\mu}m$), feed rates (100 mm/s) and three machining processes. Based on the optimum machining conditions, micro barrier rib array structures having aspect ratio 30 was machined in this study.

• IE interfaces
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• v.21 no.4
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• pp.411-417
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• 2008

An impeller is a type of high-speed rotor that is used to compress or transfer fluid under high-speed and pressure at high temperatures. The impeller is composed of an axial hub and several blades attached along the hub. The weight and shape of an impeller must be balanced, because their imbalances can cause noise and vibration, which can lead to the breakage of the impeller blades during operation. Thus, the hub and blades of an impeller are commonly machined in a 5-axis NC machine to obtain qualified surfaces. The impeller machining strategy or process plan can not be easily obtained due to the complex, overlapped and twisted shapes of impeller blades. Skillful machining process planners may generate appropriate machining strategies based on their experiences and floor data. However, in practice most shop floor data for the impeller machining is not well-structured such that it does not effectively provide a process planner with information for machining strategies and/or process plans. This paper reports the development of a case-based machining strategy support system (CBMS) that employs case-based reasoning to obtain the machining strategy of an impeller by using the existing machining strategies of the shop floor. The CBMS generates impeller machining strategies through a stepwise reasoning process considering the similarity features between the blade shapes and machining regions. A case study is provided to demonstrate that CBMS can generate useful machining strategies facilitating process planners. The developed system can simulate the tool paths of impeller machining and runs on the web.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 1997.12a
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• pp.101-101
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• 1997

Comet assay, single cell gel electrophoresis has been known as useful, rapid, simple, visual, and sensitive technique for measuring the DNA breakage in mammalian ce1ls. For evaluation of DNA damage using comet assay, early studies reported a change in comet length and intensity with DNA damage using simple visual technique, such as fluorescence microscopy with eyespiece. In recent, some workers are observing and analyzing nucleotide of comets using quantitative fluorescence image analysis system to estimate 'tail moment', which is defined as the product of the tail length and the fraction of total DNA in tail. Our laboratory also adopted the image analysis software for qualification. In addition, many of the practical features of comet assay render it potentially attractive as useful tool for molecular toxicology and carcinogenesis, because the system is already showing considerable promise as rapid predictor in both in vitro and in vivo experimental designs. Recently, the comet assay becomes a attractive technique to study of apoptosis, because apoptotic fragmentation of nuclear DNA into nucleosomal sizes can be evaluated by the comet assay. So, we attempted to apply the comet assay to studying the effect of various stress on the apoptosis-sensitive cell lines. Particularly, focusing on the hyperthermic apoptosis, we could find that heat shock(44˚C for 60 minutes) was sufficient to induced apoptosis in these cell lines. But using the highly sensitive comet assay, we could not detect DNA breaks immediately after heat shock.