• 한국자동차공학회논문집
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• 제4권4호
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• pp.190-200
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• 1996

Step-by-step time integration methods are widely used for solving structural dynamics problem. One difficult yet critical choice an analyst must make is to decide an appropriate time step size. The choice of time step size has a significant effect on solution accuracy and computational expense. The objective of this research is to derive error estimate for newly developed time integration method and develop automatic time step size control algorithm for structural dynamics. A formula for computing error tolerance is derived based on desired period resolution. An automatic time step size control strategy is proposed based on a normalized local error estimate for the generalized-α method. Numerical examples demonstrate the developed strategy satisfies general design criteria for time step size control algorithm for dynamic problem.

• Journal of Power Electronics
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• 제10권3호
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• pp.262-269
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• 2010

This paper proposes a practical design for a Cascaded H-Bridge Multilevel (CHBM) inverter based on Power Electronics Building Blocks (PEBB) and high performance control to improve current control and increase fault tolerance. It is shown that the expansion and modularization characteristics of the CHBM inverter are improved since the individual inverter modules operate more independently, when using the PEBB concept. It is also shown that the performance of current control can be improved with voltage delay compensation and the fault tolerance can be increased by using unbalance three-phase control. The proposed design and control methods are described in detail and the validity of the proposed system is verified experimentally in various industrial fields.

• Molecules and Cells
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• 제41권5호
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• pp.413-422
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• 2018

Soybean transgenic plants with ectopically expressed AtABF3 were produced by Agrobacterium-mediated transformation and investigated the effects of AtABF3 expression on drought and salt tolerance. Stable Agrobacterium-mediated soybean transformation was carried based on the half-seed method (Paz et al. 2006). The integration of the transgene was confirmed from the genomic DNA of transformed soybean plants using PCR and the copy number of transgene was determined by Southern blotting using leaf samples from $T_2$ seedlings. In addition to genomic integration, the expression of the transgenes was analyzed by RT-PCR and most of the transgenic lines expressed the transgenes introduced. The chosen two transgenic lines (line #2 and #9) for further experiment showed the substantial drought stress tolerance by surviving even at the end of the 20-day of drought treatment. And the positive relationship between the levels of AtABF3 gene expression and drought-tolerance was confirmed by qRT-PCR and drought tolerance test. The stronger drought tolerance of transgenic lines seemed to be resulted from physiological changes. Transgenic lines #2 and #9 showed ion leakage at a significantly lower level (P < 0.01) than ${\underline{n}}on-{\underline{t}}ransgenic$ (NT) control. In addition, the chlorophyll contents of the leaves of transgenic lines were significantly higher (P < 0.01). The results indicated that their enhanced drought tolerance was due to the prevention of cell membrane damage and maintenance of chlorophyll content. Water loss by transpiration also slowly proceeded in transgenic plants. In microscopic observation, higher stomata closure was confirmed in transgenic lines. Especially, line #9 had 56% of completely closed stomata whereas only 16% were completely open. In subsequent salt tolerance test, the apparently enhanced salt tolerance of transgenic lines was measured in ion leakage rate and chlorophyll contents. Finally, the agronomic characteristics of ectopically expressed AtABF3 transgenic plants ($T_2$) compared to NT plants under regular watering (every 4 days) or low rate of watering condition (every 10 days) was investigated. When watered regularly, the plant height of drought-tolerant line (#9) was shorter than NT plants. However, under the drought condition, total seed weight of line #9 was significantly higher than in NT plants (P < 0.01). Moreover, the pods of NT plants showed severe withering, and most of the pods failed to set normal seeds. All the evidences in the study clearly suggested that overexpression of the AtABF3 gene conferred drought and salt tolerance in major crop soybean, especially under the growth condition of low watering.

• 대한임상검사과학회지
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• 제36권2호
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• pp.239-244
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• 2004

Recently automatic equipment has been well popularized for the erythrocyte sedimentation rate, a widely used test, but no standard quality control has been established yet. Thus, we are going to report a case that established and applied a quality control method using the TEST 1 automatic analyzer. For internal quality control, we adopted the repeatability test through comparison with daily mean check, with a manual method using patients' specimens and with the results of a test on the previous day. In order to set the tolerance standard for each quality control method, we compared the results of 50 specimens that showed a wide range of ESR results and examined correlation and differences according to result. After setting the tolerance standard, we applied the standard at tertiary university hospitals for 120 days and investigated positive rates and re-examination rates. If the tolerance standard was exceeded, the cause was also identified. We selected a specimen of below 25 mm/hr (T1), one between 26-50 mm/hr (T2) and one of over 51 mm/hr (T3) at random. The correlation between the manual method and the automatic method was quite high (r=0.98), and it is found appropriate to set based on differences in result values below 25 mm/hr, the upper limit of the reference value, and based on differences in the percentage of result values above 25 mm/hr. Accordingly, we set the criteria for rejection above 10 mm/hr and above 20%. When the criteria were applied in the laboratory, 1.7% of specimens in the range of T1, 8.3% of those in the range of T2 and 7.5% of those in the range of T3 were rejected. Because all the rejected ones fell within the tolerance limit in re-examination calibration verification was not carried out. With the wide popularization of erythrocyte sedimentation rate analyzers that can carry out a lot of tests quickly using automatic methods, it is necessary to improve the reliability of test results by establishing internal quality control policies. We expect that an agreed standard quality control method may be established based on the method proposed in this study.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 추계학술발표회
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• pp.14-14
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• 2010

Vitamin C (ascorbic acid) is an essential component for collagen biosynthesis and also for the proper functioning of the cardiovascular system in humans. Unlike most of the animals, humans lack the ability to synthesize ascorbic acid on their own due to a mutation in the gene encoding the last enzyme of ascorbate biosynthesis. As a result, vitamin C must be obtained from dietary sources like plants. In this study, we have developed two different kinds of transgenic potato plants (Solanumtuberosum L. cv. Taedong Valley) overexpressing strawberry GalUR and mouse GLoase gene under the control of CaMV 35S promoter with increased ascorbic acid levels. Integration of the these genes in the plant genome was confirmed by PCR and Southern blotting. Ascorbic acid(AsA) levels in transgenic tubers were determined by high-performance liquid chromatography(HPLC). The over-expression of these genes resulted in 2-4 folds increase in AsA intransgenic potato and the levels of AsA were positively correlated with increased geneactivity. The transgenic lines with enhanced vitamin C content showed enhanced tolerance to abiotic stresses induced by methyl viologen(MV), NaCl or mannitol as compared to untransformed control plants. The leaf disc senescence assay showed better tolerance in transgenic lines by retaining higher chlorophyll as compared to the untransformed control plants. Present study demonstrated that the over-expression of these gene enhanced the level of AsA in potato tubers and these transgenics performed better under different abiotic stresses as compared to untransformed control. We have also investigated the mechanism of the abiotic stress tolerance upon enhancing the level of the ascorbate in transgenic potato. The transgenic potato plants overexpressing GalUR gene with enhanced accumulation of ascorbate were investigated to analyze the antioxidants activity of enzymes involved in the ascorbate-glutathione cycle and their tolerance mechanism against different abiotic stresses under invitro conditions. Transformed potato tubers subjected to various abiotic stresses induced by methyl viologen, sodium chloride and zinc chloride showed significant increase in the activities of superoxide dismutase(SOD, EC 1.15.1.1), catalase, enzymes of ascorbate-glutathione cycle enzymes such as ascorbate peroxidase(APX, EC 1.11.1.11), dehydroascorbate reductase(DHAR, EC 1.8.5.1), and glutathione reductase(GR, EC 1.8.1.7) as well as the levels of ascorbate, GSH and proline when compared to the untransformed tubers. The increased enzyme activities correlated with their mRNA transcript accumulation in the stressed transgenic tubers. Pronounced differences in redox status were also observed in stressed transgenic potato tubers that showed more tolerance to abiotic stresses when compared to untransformed tubers. From the present study, it is evident that improved to lerance against abiotic stresses in transgenic tubers is due to the increased activity of enzymes involved in the antioxidant system together with enhanced ascorbate accumulated in transformed tubers when compared to untransformed tubers. At moment we also investigating the role of enhanced reduced glutathione level for the maintenance of the methylglyoxal level as it is evident that methylglyoxal is a potent cytotoxic compound produced under the abiotic stress and the maintenance of the methylglyoxal level is important to survive the plant under stress conditions.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1989년도 한국자동제어학술회의논문집; Seoul, Korea; 27-28 Oct. 1989
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• pp.317-321
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• 1989

The FIC(Flat Integrated Circuits)is widely used for good productivity but very difficult for visual identification. The required position tolerance is 0.05mm and orientation tolerance is 0.25 degree for assembly. The research was performed to detect FIC defects and to estimate the placement of FIC within the tolerances. In this study an automatic visual system is developed, which can successfully perform a fine assembly operation using the cartesian robot.

• 대한산업공학회지
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• 제11권2호
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• pp.189-194
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• 1985

Process Capability Index is defined as a ratio between predetermined tolerance and natural tolerance of existent process ($6{\sigma}$). And it is widely used as a performance measure for quality control activities, and a criterion for process classification. This paper developed a unity-fixed process capability table for convenience to calculate process capability and showed how to use it.

• Advances in environmental research
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• 제4권1호
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• pp.1-15
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• 2015

A pot experiment was conducted to assess the effects of red pepper (Capsicum annuum) and coriander (Coriandrum sativum) on seedling growth of wheat (Triticum aestivum). The aqueous extracts treatment of red pepper and coriander showed a significant (p < 0.05) reduction in root, shoot and seedling length, number of leaves and seedling dry weight of wheat (T. aestivum) as compared to control. The inhibitory different effect on growth of wheat (T. aestivum) was directly proportional to the increasing concentration (1, 2, 3, 4 and 5%) of aqueous extracts of red pepper and coriander as compared to control treatment (0%). The root, shoot, seedling length and number of leaves of T. aestivum significantly p < 0.05 decreased at 5% concentration of red pepper as compared to control. The root, shoot and seedling growth of T. aestivum was also significantly reduced at 1, 2, 3, 4 and 5% concentration of coriander as compared to control. The root, shoot and leaves dry weight of T. aestivum at 5% coriander extract treatment concentration decreased as compared to control. The tolerance in seedlings of T. aestivum to red pepper and coriander extract treatment was dose dependent as compared to control. The seedlings of T. aestivum showed low percentage of tolerance to pepper extract treatment than coriander extract treatment.

• Journal of Electrical Engineering and Technology
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• 제13권3호
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• pp.1265-1274
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• 2018

This paper proposes a virtual flux (VF) and positive-sequence power based control strategy to improve the performance of grid-interfaced three-phase voltage source converters against unbalanced and distorted grid conditions. By using a second-order generalized integrator (SOGI) based VF observer, the proposed strategy achieves an AC voltage sensorless and grid frequency adaptive control. Aiming to realize a balanced sinusoidal line current operation, the fundamental positive-sequence component based instantaneous power is utilized as the control variable. Moreover, the fundamental negative-sequence VF feedforward and the harmonic attenuation ability of a sequence component generator are employed to further enhance the unbalance regulation ability and the harmonic tolerance of line currents, respectively. Finally, the proposed scheme is completed by combining the foregoing two elements with a predictive direct power control (PDPC). In order to verify the feasibility and validity of the proposed SOGI-VFPDPC, the scenarios of unbalanced voltage dip, higher harmonic distortion and grid frequency deviation are investigated in simulation and experimental studies. The corresponding results demonstrate that the proposed strategy ensures a balanced sinusoidal line current operation with excellent steady-state and transient behaviors under general grid conditions.

• 한국생산제조학회지
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• 제22권5호
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• pp.852-858
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• 2013

In numerical control (NC) machining, a machining error in equipment generally occurs for a variety of reasons. If there is a change in direction in the NC code, the characteristics of the automatic acceleration or deceleration function cause an overlap of each axis of the acceleration and deceleration zones, which in turn causes a shift in the actual processing path. Many studies have been conducted for error calibration of the edge as caused by automatic acceleration or deceleration in NC machining. This paper describes a geometric interpretation of the shape and processing characteristics of the operating NC device. The paper then describes a way to determine a feedrate that achieves the desired tolerance by using linear and parabolic profiles. Experiments were conducted by the validate equations using a three-axis NC machine. The results show that the machining errors were smaller than the machine resolution. The results also clearly demonstrate that the NC machine with the developed system can successfully predict machining errors induced with a change in direction.