• Molecules and Cells
• /
• 제27권4호
• /
• pp.449-458
• /
• 2009

The OsAsr1 cDNA clone was isolated from a cDNA library prepared from developing seed coats of rice (Oryza sativa L.). Low-temperature stress increased mRNA levels of OsAsr1 in both vegetative and reproductive organs. In situ analysis showed that OsAsr1 transcript was preferentially accumulated in the leaf mesophyll tissues and parenchyma cells of the palea and lemma. For transgenic rice plants that over-expressed full-length OsAsr1 cDNA in the sense orientation, the Fv/Fm values for photosynthetic efficiency were about 2-fold higher than those of wild type-segregating plants after a 24-h cold treatment. Seedlings exposed to prolonged low temperatures were more tolerant of cold stress, as demonstrated during wilting and regrowth tests. Interestingly, OsAsr1 was highly expressed in transgenic rice plants expressing the C-repeat/dehyhdration responsive element binding factor 1 (CBF1), suggesting the regulation of OsAsr1 by CBF1. Taken together, we suggest that OsAsr1 gene play an important role during temperature stress, and that this gene can be used for generating plants with enhanced cold tolerance.

• BMB Reports
• /
• 제42권11호
• /
• pp.752-757
• /
• 2009

Copper is essential but toxic in excess for aerobic organisms. Yeast transcription factor ACE1 functions as a sensor for copper and an inducer for the transcription of CUP1. In addition, ACE1 can activate the transcription of superoxide dismutase gene (sod1) in response to copper. In this study, we introduced the yeast ACE1 into Arabidopsis and analyzed its function in plant. Under high copper stress, the transgenic plants over-expressing ACE1 showed higher survival rate than the wild-type. We also found that over-expression of ACE1 in Arabidopsis increased the activities of SOD and POD, which were beneficial to the cell in copper buffering. Excess copper would suppress the expression of chlorophyll biosynthetic genes in Arabidopsis, RT-PCR analysis revealed that over-expression of ACE1 decrease the suppression. Together, our results indicate that ACE1 may play an important role in response to copper stress in Arabidopsis.

• Genes and Genomics
• /
• 제40권11호
• /
• pp.1199-1211
• /
• 2018

Soil acidification is one of major problems limiting crop growth and especially becoming increasingly serious in China owing to excessive use of nitrogen fertilizer. Only the STOP1 of Arabidopsis was identified clearly sensitive to proton rhizotoxicity and the molecular mechanism for proton toxicity tolerance of plants is still poorly understood. The main objective of this study was to investigate the transcriptomic change in plants under the low pH stress. The low pH as a single factor was employed to induce the response of the wheat seedling roots. Wheat cDNA microarray was used to identify differentially expressed genes (DEGs). A total of 1057 DEGs were identified, of which 761 genes were up-regulated and 296 were down-regulated. The greater percentage of up-regulated genes involved in developmental processes, immune system processes, multi-organism processes, positive regulation of biological processes and metabolic processes of the biological processes. The more proportion of down-regulation genes belong to the molecular function category including transporter activity, antioxidant activity and molecular transducer activity and to the extracellular region of the cellular components category. Moreover, most genes among 41 genes involved in ion binding, 17 WAKY transcription factor genes and 17 genes related to transport activity were up-regulated. KEGG analysis showed that the jasmonate signal transduction and flavonoid biosynthesis might play important roles in response to the low pH stress in wheat seedling roots. Based on the data, it is can be deduced that WRKY transcription factors might play a critical role in the transcriptional regulation, and the alkalifying of the rhizosphere might be the earliest response process to low pH stress in wheat seedling roots. These results provide a basis to reveal the molecular mechanism of proton toxicity tolerance in plants.

• 한국작물학회지
• /
• 제36권5호
• /
• pp.429-444
• /
• 1991

수도품종의 내냉성 관련인자로서, 생체막 구성물질로서 중요한 역활을하는 phospholipid의 지방산조성과 지방산 불포화비율, 생체의 가장 중요한 대사과정의 하나인 호흡에 관련된 특성인 mitochondria의 호흡활성, 광합성된 엽록소 형광강도 등 여러가지 생리적 인자들과 내냉성과의 관계를 검토하고 내냉성 품종선발과 냉해를 경감시킬 수 있는 방법을 모색하고자 관련자료를 종합정리하였다. 1. 지방산의 불포화비율은 저온처리를 함으로서 높아지는 경향을 보였으며, 내냉성정도와 비교하여 볼 때 내냉성이 강한 품종에서 불포화비율이 높고 약한 품종에서 낮아 내냉성 정도와 일치하는 경향이었다. 2. 경화처리에 의하여 인지질의 지방산조성중 palmitic acid는 감소하고 linolenic acid의 함량은 증가되었으며 지방산 불포화비율이 대조구에 비하여 19～24% 증가되어 내냉성을 증가시킨 것으로 생각되었다. 3. 수도 백화묘($25^{\circ}C$ 암상태 2주간 생육) 및 야외에서 생육시킨 유묘에서 분리한 mitochondria의 호흡활성 전이온도와 내냉성 정도와의 관계를 보면 내냉성이 강한 품종에서 전이온도가 낮았고, 약한 품종에서는 높은 경향이었다. 4. 생육시기별 mitochondria의 호흡활성 전이온도는 상풍벼 11.5～12.5$^{\circ}C$, 풍산벼 15.0～l6.$0^{\circ}C$, 샛별벼 17.0～l8.$0^{\circ}C$로서 큰 차이가 없었으나 내냉성 강, 중, 약 정도간에는 뚜렷한 차이를 보였다. 5. 엽록소 형광강도와 내냉성 정도와의 관계는 내냉성이 강한 품종에서 형광강도가 높고 약한 품종에서 낮은 경향을 보였으며 높은 산관관계를 보였다. 6. Mn을 엽면시용하므로서 호흡활성 전이온도를 0～2$^{\circ}C$ 낮추었으며 토양처리를 하였을 때 내냉성이 약한 품종에서 효과가 켰다 7 내냉성 정도와 인지질 지방산불포화도, 호흡활성 전이온도 및 엽록소 형광과의 관계에서 대체적으로 이들 세 인자와는 높은 상관을 보였으나 그중에는 독특한 반응을 보인 품종들도 있었으며, 호흡활성 전이온도는 지방용 불포화비율과는 부의 관계를, 엽록소형광과 지방산 불포화비율과는 정의 상관관계를 보였다.

• 한국응급구조학회지
• /
• 제21권3호
• /
• pp.49-58
• /
• 2017

Purpose: The purpose of this study was to validate the Moral Intelligence Checklist(MIC) of Borba (2001) modified by Kim et al. (2010) for college students majoring in health. Methods: The MIC survey was performed with 348 college students in Gongju, Chungnam. Initial MIC modified by Kim et al. (2010) consists of 7 factors (empathy, self-control, conscience, kindness, fairness, respect and tolerance) and 42 items. Data were analyzed using confirmatory factor analysis. Results: We finally analyzed with 37 items, because 5 items with estimates level below 0.5 were deleted. The Cronbach's ${\alpha}$ of 7 factors were ranged from .780 to .851. Finally, the model fit of GFI(.805), CFI(.864), RMSEA(.033) was satisfied with confirmatory factor analysis using structural equation model. Conclusion: This MIC model is suggested to be used in development of moral intelligence educational programs.

• Computers and Concrete
• /
• 제2권5호
• /
• pp.389-409
• /
• 2005

The Finite Element Analysis (FEA) is evidently a powerful tool for the analysis of structural concrete having nonlinearity and brittle failure properties. However, the result of FEA of structural concrete is sensitive to two modeling factors: the shear transfer coefficient (STC) for an open concrete crack and force convergence tolerance value (CONVTOL). Very limited work has been done to find the optimal FE Modeling (FEM) methodologies for structural concrete members strengthened with externally bonded FRP sheets. A total of 22 experimental deep beams with or without FRP flexure or/and shear strengthening systems are analyzed by nonlinear FEA using ANAYS program. For each experimental beams, an FE model with a total of 16 cases of modeling factor combinations are developed and analyzed to find the optimal FEM methodology. Two elements the SHELL63 and SOLID46 representing the material properties of FRP laminate are investigated and compared. The results of this research suggest that the optimal combination of modeling factor is STC of 0.25 and CONVTOL of 0.2. A SOLID 46 element representing the FRP strengthening system leads to better results than a SHELL 63 element does.

• 대한간호학회지
• /
• 제39권6호
• /
• pp.818-828
• /
• 2009

Purpose: This study was done to develop a cell phone addiction scale for Korean adolescents. Methods: The process included construction of a conceptual framework, generation of initial items, verification of content validity, selection of secondary items, preliminary study, and extraction of final items. The participants were 577 adolescents in two middle schools and three high schools. Item analysis, factor analysis, criterion related validity, and internal consistency were used to analyze the data. Results: Twenty items were selected for the final scale, and categorized into 3 factors explaining 55.45% of total variance. The factors were labeled as withdrawal/tolerance (7 items), life dysfunction (6 items), and compulsion/persistence (7 items). The scores for the scale were significantly correlated with self-control, impulsiveness, and cell phone use. Cronbach's alpha coefficient for the 20 items was .92. Scale scores identified students as cell phone addicted, heavy users, or average users. Conclusion: The above findings indicate that the cell phone addiction scale has good validity and reliability when used with Korean adolescents.

• 아동학회지
• /
• 제28권6호
• /
• pp.37-52
• /
• 2007

This study was planned to develop an Internet Game Addiction Scale for 9- to 12-year-old Korean children. Based on literature review and analysis of counseling cases, the concept of the internet game addiction was defined. Items of the scale were developed and then the content validity of the scale was examined by 20 professionals. Then a preliminary survey of 188 3rd to 6th grade students was made, and the main survey of 699 3rd to 6th grade students throughout Korea. The main results are as follows; [I] Internet Game Addiction Scale consists of 3 factors with 20 items : game-oriented life(6 items), tolerance & mastery loss(7 items), and withdrawal & emotional experience(7 items); [II] appropriateness of item mean and standard deviation was proved, a 3-factor structure confirmed through factorial analysis and cross validation; [III] appropriateness of the scale confirmed through criterion validity and reliability.

• Journal of Electrical Engineering and Technology
• /
• 제13권6호
• /
• pp.2262-2267
• /
• 2018

In electric machine design, there is a large computation cost for finite element analyses (FEA) when analyzing nonlinear characteristics in the machine Therefore, for the optimal design of an electric machine, designers commonly use an optimization algorithm capable of excellent convergence performance. However, robustness consideration, as this factor can guarantee machine performances capabilities within design uncertainties such as the manufacturing tolerance or external perturbations, is essential during the machine design process. Moreover, additional FEA is required to search robust optimum. To address this issue, this paper proposes a computationally efficient robust optimization algorithm. To reduce the computational burden of the FEA, the proposed algorithm employs a useful technique which termed static analysis assisted technique (SAAT). The proposed method is verified via the effective robust optimal design of electric machine to reduce cogging torque at a reasonable computational cost.

• 대한기계학회논문집
• /
• 제18권5호
• /
• pp.1347-1354
• /
• 1994

This paper deals with the experimental research, including measurement and analysis and field survey, on the causes of occurring noise defective gear boxes in hoist production plant in order to reduce the fraction of their occurrence. In this reserch following investigations are performed : measurement and gear-boxes, examination of each machining process of production, measurement and analysis of dimensional accuracy of each part, comparative vibration test with exchanging inaccurate parts. From these investigations, it is found that the machining accuracy of pinion gear tooth thickness is the most sensitive factor of noise problem. By maintaining the tooth thickness error within 0.05 mm tolerance in the gear cutting process, the fraction of noise defective gear-boxes are greatly reduced to less than 2%, where the usual rate of it has been 20-50%.