• 공업화학
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• 제19권3호
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• pp.326-331
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• 2008

본 연구의 목표는 고체기질 표면에 Na 이온이 포함된 비양론적 인회석 코팅을 형성하는 기술을 개발하는 것이다. 나트륨염 농도가 다른 조건에서 형성된 인회석 코팅은 Na 이온이 존재하는 경우 표면 morphology, 화학적 상태 그리고 Ca/P 비율 등에 상당한 영향을 미치는 것으로 나타났다. 이러한 성질들의 변화는 Na 이온의 농도가 0.01 mM로 증가될 때까지 지속되었다. 칼슘과 인의 비율은 2.18에서 2.03 정도까지 변화되었으며, 이는 합성된 비양론적 인회석이 칼슘이 풍부한 조성을 가지고 있음을 나타내고 있다. 모든 시료들의 구조는 저결정성 구조를 갖는 것으로 나타났다. 인회석 코팅층 내에 Na 이온이 존재하는 경우, 인간 조골세포의 세포주인 SaOS-2 세포의 부착이 상당히 증가하였다. 그러나 이 세포들의 증식은 Na 이온 농도가 증가함에 따라 감소하는 것으로 나타났다. 이러한 상반된 세포반응은 SaOS-2 세포와 인회석 표면간의 작용이 세포신호전달을 포함한 세포내 기전에 상당한 변화를 초래했기 때문일 것으로 사료된다.

• Journal of Periodontal and Implant Science
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• 제27권4호
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• pp.767-783
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• 1997

Guided tissue regeneration, bone graft procedures, and application of growth factors have been used to regenerate lost periodontal tissues. Recently, enamel matrix derivative has been introduced into periodontal regeneration procedures in expectation of promoting new bone and cementum formation. The purpose' of this study was to evaluate the effect of enamel matrix derivative in 1-wall intrabony defects in beagle dogs. For this purpose, each dog was anesthesized using intravenous anesthesia and mandibular 1st, 3rd premolars were extracted. 2 months later, the 1-wall intrabony defects(mesio-distal width: 4mm, depth: 4mm) were created on the distal side of 2nd premolars and mesial side of 4th premolars. The control group was treated with debridement alone, and experimental group was treated with debridement and enamel matrix derivative application. The healing processes were histologically and histometrically observed after 8 weeks and the results were as follows : 1. The length of junctional epithelium was $0.94{\pm}0.80mm$ in the control group, $0.57{\pm}0.42mm$ in the experimental group, with no statistically significant difference between groups. 2. The connective tissue attachment was $1.36{\pm}0.98mm$ in the control group. $0.38{\pm}0.43mm$ in the experimental group, with statistically significant difference between groups(P<0.05). 3. The new cementum formation was $2.49{\pm}1.06mm$ in the control group, $3.59{\pm}0.74mm$ in the experimental group. with statistically significant difference between groups(P<0.05). 4. The new bone formation was $1.92{\pm}0.97mm$ in the control group, $2.32{\pm}0.59mm$ in the experimental group. with no statistically significant difference between groups. Within the limitation to this study protocol, enamel matrix derivative application in 1-wall intrabony defect enhanced new cementum formation. Although there was no statistically significant difference, enamel matrix derivative also seems to be effective in inhibition of apical migration of junctional epithelium and new bone formation.

• 한국발생생물학회지:발생과생식
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• 제17권3호
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• pp.215-220
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• 2013

Recently, the RNA/DNA-binding protein FUS, Fused in sarcoma, was shown to play a role in growth, differentiation, and morphogenesis in vertebrates. Because little is known about Fus, we investigated its expression pattern in murine tooth development. In situ hybridization of mouse mandibles at specific developmental stages was performed with a DIG-labeled RNA probe. During early tooth development, Fus was detected in the dental epithelium and dental mesenchyme at 11 days postcoitum (dpc) and 12 dpc. From 14 dpc, Fus was strongly expressed in the dental papilla and the cervical loop of the dental epithelium. At postnatal day 4 (PN4), Fus expression was observed in the odontoblasts, ameloblasts, the proliferation zone of the pulp, and the cervical loop. At PN14, the expression pattern of Fus was found to be maintained in the odontoblasts and the proliferation zone of the pulp. Furthermore, Fus expression was especially strong in the Hertwig's epithelial root sheath (HERS). Therefore, this study suggests that Fus may play a role in the HERS during root development.

• Journal of Periodontal and Implant Science
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• 제25권3호
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• pp.518-528
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• 1995

The immunohistochemical study has been performed on the distribution of receptors for various growth factors in the newly forming granulation tissues following the guided tissue regeneration procedures. Two specimens from 2 different patients were collected from the newly forming granulation tissues at 2 weeks following GTR procedures using Gore-tex menbrane and rubber dam, respectively. For immunohistochemical localization of each recptor, anti-platelet-derived growth factor $receptor-{\alpha}$, anti-platelet-derived growth factor $receptor-{\beta}$. anti-insulin-like growth factor receptor, anti-basic fibroblast growth factor receptor, anti-transforming growth $factor-{\beta}$ receptor and anti-fibronectin receptor were incubated onto the specimens as primary antibodies. After the reaction, FITC-conjugated second antibodies have been applied. When the total numbers of immunoreactive cells and the true positive cells were counted, there were high variability among receptors tested in the present study. The mean number of immunoreactive cells were highest in the case for anti-IFG-1 receptor. However the number of true positive cells were highest in the case for $TGF-{\beta}$ receptor. The present investigation indicated that the receptor for $TGF-{\beta}$ were stongly expressed in the newly forming granulation tissues following the guided tissue regeneration therapy.

• 식물조직배양학회지
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• 제21권2호
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• pp.65-68
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• 1994

한방에서 항암제로 사용되고 있는 약용식물들로부터 이 차대사산물을 생산하기 위한 시도로서 바위솔의 조직배양을 시도하였다. 바위솔의 부위별 조직, 즉 잎, 줄기 및 화주 조직을 이용하여 캘러스 유기를 시도하였다. 줄기조직과 화주조직에서는 캘러스가 유기되었으나 잎 조직으로부터는 캘러스가 유기되지 많았다. 따라서 캘러스 유기가 가장 왕성하게 일어나는 줄기조직으로부터 유기된 캘러스를 이용하여 식물체 재분화를 시도하였다. 캘러스 유기에는 MS배지에 4 mg/L 2,4-D와 2 mg/L BAP를 첨가하였을 때 가장 효과적이었으며, 잎 조직으로부터는 캘러스형성이 되지 않았다. 식물체 재분화에 있어서 줄기분화는 MS배지 0.5 mg/L와 BAP 2 mg/L를 첨가한 배지에서 가장 높은 분화율을 나타내었으며, 뿌리형성은 2,4-D 1 mg/L와 kinetin 1∼3 mg/L를 첨가한 배지에서만 5%의 분화율을 나타내었다. 뿌리형성에 대한 2,4-D의 적정 농도에 대한 연구 및 뿌리 발생시 생성되는 적색물질의 규명에 대한 연구가 진행되어야 할 것이다. 완전한 식물체로 재분화가 일어나는데는 약 14 주의 기간이 소요되었다.

• 대한치과의사협회지
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• 제54권12호
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• pp.985-995
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• 2016

Titanium (Ti) has been widely used for dental implant due to great biocompatibility and bonding ability against natural alveolar bone. A lot of titanium surface modification has been introduced in dentistry and, among them, methods to introduce micro/nano-roughened surface were considered as clinically approved strategy for accelerating osseointegration of Ti dental implant. To have synergetic effect with topography oriented favors in cell attachment, chair-side surface treatment with reproducibility of micro/nano-topography is introduced as next strategy to further enhance cellular functionalities. Extensive research has been investigated to study the potential of micro/nano-topography preserved chair-side surface treatment for Ti dental implant. This review will discuss ultraviolet, low level of laser therapy and non-thermal atmospheric pressure plasma on Ti dental implant with micro/nano-topography as next generation of surface treatment due to its abilities to induce super-hydrophilicity or biofunctionality without change of topographical cues.

• Journal of Periodontal and Implant Science
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• 제34권3호
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• pp.683-698
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• 2004

One of the bone substitutes now in routine use, deproteinized bovine bone mineral(DBBM), is regarded as resorbable and osteoconductive, but some studies refute this. The present study was performed to evaluate the effects of DBBM on guided bone regeneration using titanium membrane on the calvaria of rabbit. At 2 weeks, 4 weeks, 8 weeks, and 12 weeks after surgery, the animal was scrificed. Non-decalcified specimens were produced for histologic analysis. The results of this study were as follows : 1. Titanium membrane was biocompatible and capable of space-maintaining, but there was ingrowth of soft tissue through the pore of titanium membrane. 2. There was no resorption or reduction of DBBM with time. 3. Some of the DBBM particles were combined with newly formed bone. But, apart from host bone, a great part of the particles were surrounded by connective tissue. 4. The bone formation was slight vertically and restricted to superficial area of host bone. Whithin the above results, DBBM dose not appear to contribute to bone formation. DBBM may disturb the migration and proliferation of mesenchymal cell derived from host bone and increase the growth of connective tissue. Therefore, careful caution is needed on selection of bone graft material and surgical protocol at guided bone regeneration for implant placement.

• Journal of Periodontal and Implant Science
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• 제36권2호
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• pp.427-434
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• 2006

The form of furcation influence both the pathogenesis of periodontal destruction and therapeutic results. The present study was performed to evaluate the effect of root trunk length on clinical outcomes of guided tissue regeneration. Total 30 mandibular first molars were evaluated in this study. Probing pocket depth, clinical attachment level, vertical defect depth and horizontal defect depth were measured at baseline and 6 month after GTR. Correlation coefficients between root trunk length and other clinical measurement were analyzed. The results of this study were as follows 1. The mean root trunk length in lower 1st molar was 2.15 mm. 2. Probing pocket depth, clinical attachment level, vertical defect depth and horizontal defect depth were significantly reduced at 6 month postoperatively compared to values of baseline 3. Correlation coefficient between root trunk length and vertical defect depth at baseline was 0.406 showing the positive correlation 4. Correlation coefficient between root trunk length and horizontal defect depth at baseline was -0.463 showing the negative correlation. 5. Correlation coefficient between root trunk length and decrease of horizontal defect depth after GTR was 0.654 showing the positive correlation. In conclusion, the root trunk length maybe effector for clinical outcome after guided tissue regeneration.

• The Korean Journal of Physiology and Pharmacology
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• 제11권1호
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• pp.31-36
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• 2007

To elucidate a potential molecular link between diabetes and atherosclerosis, we investigated the role of Janus tyrosine kinase(JAK) for NAD(P)H oxidase-derived superoxide generation in the enhanced proliferative capacity of vascular smooth muscle cells(VSMC) of Otsuka Long-Evans Tokushima Fatty(OLETF) rat, an animal model of type 2 diabetes. An enhanced proliferative response to 10% fetal bovine serum(FBS) and superoxide generation with an increased NAD(P)H oxidase activity were observed in diabetic(OLETF) VSMC. Both the enhanced proliferation and superoxide generation in diabetic VSMC were significantly attenuated by AG490, JAK2 inhibitor, and PP2, Src kinase inhibitor. Tyrosine phosphorylation of proteins in diabetic VSMC, especially JAK2, was increased compared to control VSMC. Furthermore, the enhanced NAD(P)H oxidase activity in diabetic VSMC was significantly attenuated by AG490 in a dose-dependent manner. Together, these results indicate that the signal pathway which leads to diabetes-associated activation of Src kinase/JAK is critically involved in the diabetic VSMC proliferation through NAD(P)H oxidase activation and superoxide generation.

• 한국작물학회지
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• 제37권5호
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• pp.419-424
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• 1992

콩에서의 조직배양 가능성을 알아보고자 콩 유묘를 기내에서 배양시킨 뒤, 여러 부위를 채취하여 각기 다른 배지 치상한 결과는 다음과 같다. 1. 줄기정단조직 배양에서는 대부분 shoot가 분화되어 완전한 식물체를 얻을 수 있었다. 2. 자엽절 조직배양에서는 multiple shoots가 나타나 대량증식에 유용했으나 배지에 따른 영향이 컸다. 3. 배축 및 상배축 조직배양에서는 callus만 분화되엇다. 4. 엽조직배양에서는 callus 분화 후 계대배양했을 때 shoot가 분화되었으나 완전한 식물체를 얻기는 힘들었다.